Endophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-04

AUTHORS

Antônio Sérgio Ferreira Filho, Maria Carolina Quecine, Andréa Cristina Bogas, Priscilla de Barros Rossetto, Andre Oliveira de Souza Lima, Paulo Teixeira Lacava, João Lúcio Azevedo, Welington Luiz Araújo

ABSTRACT

Based on the premise of symbiotic control, we genetically modified the citrus endophytic bacterium Methylobacterium extorquens, strain AR1.6/2, and evaluated its capacity to colonize a model plant and its interaction with Xylella fastidiosa, the causative agent of Citrus Variegated Chlorosis (CVC). AR1.6/2 was genetically transformed to express heterologous GFP (Green Fluorescent Protein) and an endoglucanase A (EglA), generating the strains ARGFP and AREglA, respectively. By fluorescence microscopy, it was shown that ARGFP was able to colonize xylem vessels of the Catharanthus roseus seedlings. Using scanning electron microscopy, it was observed that AREglA and X. fastidiosa may co-inhabit the C. roseus vessels. M. extorquens was observed in the xylem with the phytopathogen X. fastidiosa, and appeared to cause a decrease in biofilm formation. AREglA stimulated the production of resistance protein, catalase, in the inoculated plants. This paper reports the successful transformation of AR1.6/2 to generate two different strains with a different gene each, and also indicates that AREglA and X. fastidiosa could interact inside the host plant, suggesting a possible strategy for the symbiotic control of CVC disease. Our results provide an enhanced understanding of the M. extorquens-X. fastidiosa interaction, suggesting the application of AR1.6/2 as an agent of symbiotic control. More... »

PAGES

1475-1481

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11274-011-0949-2

DOI

http://dx.doi.org/10.1007/s11274-011-0949-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010409010

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22805929


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibiosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Catharanthus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cellulase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endophytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metabolic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methylobacterium extorquens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron, Scanning", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Seedlings", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xylella", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xylem", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universidade de Passo Fundo", 
          "id": "https://www.grid.ac/institutes/grid.412279.b", 
          "name": [
            "Department of Phytopathology, University of Passo Fundo (UPF), BR 285, PO BOX 611, 99052-900, Passo Fundo, RS, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ferreira Filho", 
        "givenName": "Ant\u00f4nio S\u00e9rgio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Department of Genetics, Escola Superior de Agricultura \u201cLuiz de Queiroz\u201d, University of S\u00e3o Paulo, 11 P\u00e1dua Dias Av., PO BOX 83, 13400-970, Piracicaba, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Quecine", 
        "givenName": "Maria Carolina", 
        "id": "sg:person.0765617335.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765617335.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Department of Genetics, Escola Superior de Agricultura \u201cLuiz de Queiroz\u201d, University of S\u00e3o Paulo, 11 P\u00e1dua Dias Av., PO BOX 83, 13400-970, Piracicaba, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bogas", 
        "givenName": "Andr\u00e9a Cristina", 
        "id": "sg:person.01116746575.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116746575.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Laboratory of Biotechnology, Centro de Energia Nuclear na Agricultura, University of S\u00e3o Paulo, 303 Centen\u00e1rio Av., PO BOX 96, 13416 000, Piracicaba, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rossetto", 
        "givenName": "Priscilla de Barros", 
        "id": "sg:person.01160632774.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160632774.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Center of Science Tecnology of Earh and Sea, University of Vale do Itaja\u00ed, 458 Uruguai Street, 14, 88302-202, Itaja\u00ed, SC, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lima", 
        "givenName": "Andre Oliveira de Souza", 
        "id": "sg:person.012103264731.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012103264731.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Alfenas", 
          "id": "https://www.grid.ac/institutes/grid.411180.d", 
          "name": [
            "Institute of Natural Science, Federal University of Alfenas, 37130-000, Alfenas, MG, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lacava", 
        "givenName": "Paulo Teixeira", 
        "id": "sg:person.01310553574.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310553574.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Department of Genetics, Escola Superior de Agricultura \u201cLuiz de Queiroz\u201d, University of S\u00e3o Paulo, 11 P\u00e1dua Dias Av., PO BOX 83, 13400-970, Piracicaba, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Azevedo", 
        "givenName": "Jo\u00e3o L\u00facio", 
        "id": "sg:person.010472231140.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010472231140.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Department of Microbiology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, Av. Prof. Lineu Prestes, 1374 - Ed. Biom\u00e9dicas II, Cidade Universit\u00e1ria, 05508-900, S\u00e3o Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ara\u00fajo", 
        "givenName": "Welington Luiz", 
        "id": "sg:person.0725467340.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725467340.10"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1046/j.1472-765x.2001.01000.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002422328"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micres.2006.08.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003551904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.2001.tb10836.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004024826"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.2001.tb10836.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004024826"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.2000.tb09423.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005958673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.2000.tb09423.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005958673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1399-3054.1998.1040217.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007779276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11274-010-0344-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008373435", 
          "https://doi.org/10.1007/s11274-010-0344-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11274-010-0344-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008373435", 
          "https://doi.org/10.1007/s11274-010-0344-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35018003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009850185", 
          "https://doi.org/10.1038/35018003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35018003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009850185", 
          "https://doi.org/10.1038/35018003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/m97-131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012451714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1998.tb13175.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013088201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.1998.tb13175.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013088201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.71.11.7245-7252.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013894388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-004-1740-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015860728", 
          "https://doi.org/10.1007/s00253-004-1740-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01576010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018869550", 
          "https://doi.org/10.1007/bf01576010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12275-008-0303-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026128208", 
          "https://doi.org/10.1007/s12275-008-0303-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12275-008-0303-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026128208", 
          "https://doi.org/10.1007/s12275-008-0303-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/w05-142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026457565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1078/0723-2020-00263", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026647782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0176-1617(11)81790-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030991316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01566883", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031136458", 
          "https://doi.org/10.1007/bf01566883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.68.10.4906-4914.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037042168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.phyto.36.1.453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048695453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0378-1097(01)00348-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054654764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/phyto-75-264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060107471"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-147-8-2065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060370781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074466857", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074466883", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082140684", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-04", 
    "datePublishedReg": "2012-04-01", 
    "description": "Based on the premise of symbiotic control, we genetically modified the citrus endophytic bacterium Methylobacterium extorquens, strain AR1.6/2, and evaluated its capacity to colonize a model plant and its interaction with Xylella fastidiosa, the causative agent of Citrus Variegated Chlorosis (CVC). AR1.6/2 was genetically transformed to express heterologous GFP (Green Fluorescent Protein) and an endoglucanase A (EglA), generating the strains ARGFP and AREglA, respectively. By fluorescence microscopy, it was shown that ARGFP was able to colonize xylem vessels of the Catharanthus roseus seedlings. Using scanning electron microscopy, it was observed that AREglA and X. fastidiosa may co-inhabit the C. roseus vessels. M. extorquens was observed in the xylem with the phytopathogen X. fastidiosa, and appeared to cause a decrease in biofilm formation. AREglA stimulated the production of resistance protein, catalase, in the inoculated plants. This paper reports the successful transformation of AR1.6/2 to generate two different strains with a different gene each, and also indicates that AREglA and X. fastidiosa could interact inside the host plant, suggesting a possible strategy for the symbiotic control of CVC disease. Our results provide an enhanced understanding of the M. extorquens-X. fastidiosa interaction, suggesting the application of AR1.6/2 as an agent of symbiotic control.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11274-011-0949-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096771", 
        "issn": [
          "0959-3993", 
          "1573-0972"
        ], 
        "name": "World Journal of Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "28"
      }
    ], 
    "name": "Endophytic Methylobacterium extorquens expresses a heterologous \u03b2-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa", 
    "pagination": "1475-1481", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3196f020b98d640c1102188e6f9d514d0c8c62e8583304fa690008e5bb0b9b96"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22805929"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9012472"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11274-011-0949-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010409010"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11274-011-0949-2", 
      "https://app.dimensions.ai/details/publication/pub.1010409010"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T17:33", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000520.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11274-011-0949-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11274-011-0949-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11274-011-0949-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11274-011-0949-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11274-011-0949-2'


 

This table displays all metadata directly associated to this object as RDF triples.

252 TRIPLES      21 PREDICATES      66 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11274-011-0949-2 schema:about N04c1d5ea143b4837b349a8afa4e269bb
2 N1d07fb5b257f4b4498f5274bec408f73
3 N2eb5df2135d642d0bcb37a19ec513c2b
4 N3539e8733fd443c1a1a0dd6abdc7f3ee
5 N56f189121bfd4b64bd5647c46265cd20
6 N9075e3a121e746bfa7755182a97a3fe4
7 Nb6d26aa6c7e54b58a80616ce0fa60528
8 Nbb929bece937490ab63bc00bb5a0f27e
9 Ne02e568e7c584f688bcb87cc359ca042
10 Nf93ae39cb8554d5cba6fc613d2ebd1df
11 Nf99442810170408dbd2b5e3799157816
12 anzsrc-for:06
13 anzsrc-for:0607
14 schema:author N2047db210dd144c69af8071772d84ff3
15 schema:citation sg:pub.10.1007/bf01566883
16 sg:pub.10.1007/bf01576010
17 sg:pub.10.1007/s00253-004-1740-1
18 sg:pub.10.1007/s11274-010-0344-4
19 sg:pub.10.1007/s12275-008-0303-z
20 sg:pub.10.1038/35018003
21 https://app.dimensions.ai/details/publication/pub.1074466857
22 https://app.dimensions.ai/details/publication/pub.1074466883
23 https://app.dimensions.ai/details/publication/pub.1082140684
24 https://doi.org/10.1016/j.micres.2006.08.009
25 https://doi.org/10.1016/s0176-1617(11)81790-6
26 https://doi.org/10.1016/s0378-1097(01)00348-2
27 https://doi.org/10.1034/j.1399-3054.1998.1040217.x
28 https://doi.org/10.1046/j.1472-765x.2001.01000.x
29 https://doi.org/10.1078/0723-2020-00263
30 https://doi.org/10.1094/phyto-75-264
31 https://doi.org/10.1099/00221287-147-8-2065
32 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
33 https://doi.org/10.1111/j.1574-6968.1998.tb13175.x
34 https://doi.org/10.1111/j.1574-6968.2000.tb09423.x
35 https://doi.org/10.1111/j.1574-6968.2001.tb10836.x
36 https://doi.org/10.1128/aem.68.10.4906-4914.2002
37 https://doi.org/10.1128/aem.71.11.7245-7252.2005
38 https://doi.org/10.1139/m97-131
39 https://doi.org/10.1139/w05-142
40 https://doi.org/10.1146/annurev.phyto.36.1.453
41 schema:datePublished 2012-04
42 schema:datePublishedReg 2012-04-01
43 schema:description Based on the premise of symbiotic control, we genetically modified the citrus endophytic bacterium Methylobacterium extorquens, strain AR1.6/2, and evaluated its capacity to colonize a model plant and its interaction with Xylella fastidiosa, the causative agent of Citrus Variegated Chlorosis (CVC). AR1.6/2 was genetically transformed to express heterologous GFP (Green Fluorescent Protein) and an endoglucanase A (EglA), generating the strains ARGFP and AREglA, respectively. By fluorescence microscopy, it was shown that ARGFP was able to colonize xylem vessels of the Catharanthus roseus seedlings. Using scanning electron microscopy, it was observed that AREglA and X. fastidiosa may co-inhabit the C. roseus vessels. M. extorquens was observed in the xylem with the phytopathogen X. fastidiosa, and appeared to cause a decrease in biofilm formation. AREglA stimulated the production of resistance protein, catalase, in the inoculated plants. This paper reports the successful transformation of AR1.6/2 to generate two different strains with a different gene each, and also indicates that AREglA and X. fastidiosa could interact inside the host plant, suggesting a possible strategy for the symbiotic control of CVC disease. Our results provide an enhanced understanding of the M. extorquens-X. fastidiosa interaction, suggesting the application of AR1.6/2 as an agent of symbiotic control.
44 schema:genre research_article
45 schema:inLanguage en
46 schema:isAccessibleForFree false
47 schema:isPartOf N0d5d0fbb3ec8404bbfe3fb24a045acc9
48 N91d17853bd9445a58467851b26bc1a61
49 sg:journal.1096771
50 schema:name Endophytic Methylobacterium extorquens expresses a heterologous β-1,4-endoglucanase A (EglA) in Catharanthus roseus seedlings, a model host plant for Xylella fastidiosa
51 schema:pagination 1475-1481
52 schema:productId N038e3a2e06f44974bb4a53cfbc2b2c31
53 N1a091e31329c456c8175e3a558860de6
54 N97f3773eca404402a32e5bf6b13f0d74
55 N9930bb1469934aa18090c4e62ef78aac
56 Nee2928390803479d8458337b888d2d96
57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010409010
58 https://doi.org/10.1007/s11274-011-0949-2
59 schema:sdDatePublished 2019-04-10T17:33
60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
61 schema:sdPublisher Ne5a3550a432c423abce61d00f3b8a1da
62 schema:url http://link.springer.com/10.1007%2Fs11274-011-0949-2
63 sgo:license sg:explorer/license/
64 sgo:sdDataset articles
65 rdf:type schema:ScholarlyArticle
66 N038e3a2e06f44974bb4a53cfbc2b2c31 schema:name nlm_unique_id
67 schema:value 9012472
68 rdf:type schema:PropertyValue
69 N04c1d5ea143b4837b349a8afa4e269bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Plant Diseases
71 rdf:type schema:DefinedTerm
72 N0d5d0fbb3ec8404bbfe3fb24a045acc9 schema:volumeNumber 28
73 rdf:type schema:PublicationVolume
74 N187d05eb934849f1b364d2aed182aa45 schema:affiliation https://www.grid.ac/institutes/grid.412279.b
75 schema:familyName Ferreira Filho
76 schema:givenName Antônio Sérgio
77 rdf:type schema:Person
78 N1a091e31329c456c8175e3a558860de6 schema:name readcube_id
79 schema:value 3196f020b98d640c1102188e6f9d514d0c8c62e8583304fa690008e5bb0b9b96
80 rdf:type schema:PropertyValue
81 N1d07fb5b257f4b4498f5274bec408f73 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Cellulase
83 rdf:type schema:DefinedTerm
84 N2047db210dd144c69af8071772d84ff3 rdf:first N187d05eb934849f1b364d2aed182aa45
85 rdf:rest N8e3e34a886ed494da4b649d8125de794
86 N2612cf833b03486eb74a23c2a9b9cfe8 schema:name Center of Science Tecnology of Earh and Sea, University of Vale do Itajaí, 458 Uruguai Street, 14, 88302-202, Itajaí, SC, Brazil
87 rdf:type schema:Organization
88 N263871fa12e943278c7504992ced64f4 rdf:first sg:person.01310553574.91
89 rdf:rest Na88a12f3f0344503840f4e79111a5320
90 N2eb5df2135d642d0bcb37a19ec513c2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Xylella
92 rdf:type schema:DefinedTerm
93 N3539e8733fd443c1a1a0dd6abdc7f3ee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Seedlings
95 rdf:type schema:DefinedTerm
96 N56f189121bfd4b64bd5647c46265cd20 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Metabolic Engineering
98 rdf:type schema:DefinedTerm
99 N88c449f85d014957a9c96887c1e4746e rdf:first sg:person.01160632774.22
100 rdf:rest Nbb73f8e658d843b7ba2c12c8729b59b5
101 N8e3e34a886ed494da4b649d8125de794 rdf:first sg:person.0765617335.92
102 rdf:rest Nadbca0598dc44beb8025d6ae613d125c
103 N9075e3a121e746bfa7755182a97a3fe4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Endophytes
105 rdf:type schema:DefinedTerm
106 N91d17853bd9445a58467851b26bc1a61 schema:issueNumber 4
107 rdf:type schema:PublicationIssue
108 N970f1afab2574832994f2a46bda62164 rdf:first sg:person.0725467340.10
109 rdf:rest rdf:nil
110 N97f3773eca404402a32e5bf6b13f0d74 schema:name dimensions_id
111 schema:value pub.1010409010
112 rdf:type schema:PropertyValue
113 N9930bb1469934aa18090c4e62ef78aac schema:name pubmed_id
114 schema:value 22805929
115 rdf:type schema:PropertyValue
116 Na88a12f3f0344503840f4e79111a5320 rdf:first sg:person.010472231140.14
117 rdf:rest N970f1afab2574832994f2a46bda62164
118 Nadbca0598dc44beb8025d6ae613d125c rdf:first sg:person.01116746575.80
119 rdf:rest N88c449f85d014957a9c96887c1e4746e
120 Nb6d26aa6c7e54b58a80616ce0fa60528 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Microscopy, Electron, Scanning
122 rdf:type schema:DefinedTerm
123 Nbb73f8e658d843b7ba2c12c8729b59b5 rdf:first sg:person.012103264731.94
124 rdf:rest N263871fa12e943278c7504992ced64f4
125 Nbb929bece937490ab63bc00bb5a0f27e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Xylem
127 rdf:type schema:DefinedTerm
128 Ne02e568e7c584f688bcb87cc359ca042 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Methylobacterium extorquens
130 rdf:type schema:DefinedTerm
131 Ne5a3550a432c423abce61d00f3b8a1da schema:name Springer Nature - SN SciGraph project
132 rdf:type schema:Organization
133 Nee2928390803479d8458337b888d2d96 schema:name doi
134 schema:value 10.1007/s11274-011-0949-2
135 rdf:type schema:PropertyValue
136 Nf93ae39cb8554d5cba6fc613d2ebd1df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Antibiosis
138 rdf:type schema:DefinedTerm
139 Nf99442810170408dbd2b5e3799157816 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Catharanthus
141 rdf:type schema:DefinedTerm
142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biological Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
146 schema:name Plant Biology
147 rdf:type schema:DefinedTerm
148 sg:journal.1096771 schema:issn 0959-3993
149 1573-0972
150 schema:name World Journal of Microbiology and Biotechnology
151 rdf:type schema:Periodical
152 sg:person.010472231140.14 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
153 schema:familyName Azevedo
154 schema:givenName João Lúcio
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010472231140.14
156 rdf:type schema:Person
157 sg:person.01116746575.80 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
158 schema:familyName Bogas
159 schema:givenName Andréa Cristina
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116746575.80
161 rdf:type schema:Person
162 sg:person.01160632774.22 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
163 schema:familyName Rossetto
164 schema:givenName Priscilla de Barros
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160632774.22
166 rdf:type schema:Person
167 sg:person.012103264731.94 schema:affiliation N2612cf833b03486eb74a23c2a9b9cfe8
168 schema:familyName Lima
169 schema:givenName Andre Oliveira de Souza
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012103264731.94
171 rdf:type schema:Person
172 sg:person.01310553574.91 schema:affiliation https://www.grid.ac/institutes/grid.411180.d
173 schema:familyName Lacava
174 schema:givenName Paulo Teixeira
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310553574.91
176 rdf:type schema:Person
177 sg:person.0725467340.10 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
178 schema:familyName Araújo
179 schema:givenName Welington Luiz
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725467340.10
181 rdf:type schema:Person
182 sg:person.0765617335.92 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
183 schema:familyName Quecine
184 schema:givenName Maria Carolina
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765617335.92
186 rdf:type schema:Person
187 sg:pub.10.1007/bf01566883 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031136458
188 https://doi.org/10.1007/bf01566883
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/bf01576010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018869550
191 https://doi.org/10.1007/bf01576010
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/s00253-004-1740-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015860728
194 https://doi.org/10.1007/s00253-004-1740-1
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/s11274-010-0344-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008373435
197 https://doi.org/10.1007/s11274-010-0344-4
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/s12275-008-0303-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1026128208
200 https://doi.org/10.1007/s12275-008-0303-z
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/35018003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009850185
203 https://doi.org/10.1038/35018003
204 rdf:type schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1074466857 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1074466883 schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1082140684 schema:CreativeWork
208 https://doi.org/10.1016/j.micres.2006.08.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003551904
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/s0176-1617(11)81790-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030991316
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/s0378-1097(01)00348-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054654764
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1034/j.1399-3054.1998.1040217.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007779276
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1046/j.1472-765x.2001.01000.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002422328
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1078/0723-2020-00263 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026647782
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1094/phyto-75-264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060107471
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1099/00221287-147-8-2065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060370781
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1111/j.1574-6968.1998.tb13175.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013088201
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1111/j.1574-6968.2000.tb09423.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005958673
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1111/j.1574-6968.2001.tb10836.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004024826
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1128/aem.68.10.4906-4914.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037042168
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1128/aem.71.11.7245-7252.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013894388
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1139/m97-131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012451714
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1139/w05-142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026457565
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1146/annurev.phyto.36.1.453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048695453
241 rdf:type schema:CreativeWork
242 https://www.grid.ac/institutes/grid.11899.38 schema:alternateName University of Sao Paulo
243 schema:name Department of Genetics, Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo, 11 Pádua Dias Av., PO BOX 83, 13400-970, Piracicaba, SP, Brazil
244 Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374 - Ed. Biomédicas II, Cidade Universitária, 05508-900, São Paulo, SP, Brazil
245 Laboratory of Biotechnology, Centro de Energia Nuclear na Agricultura, University of São Paulo, 303 Centenário Av., PO BOX 96, 13416 000, Piracicaba, SP, Brazil
246 rdf:type schema:Organization
247 https://www.grid.ac/institutes/grid.411180.d schema:alternateName Federal University of Alfenas
248 schema:name Institute of Natural Science, Federal University of Alfenas, 37130-000, Alfenas, MG, Brazil
249 rdf:type schema:Organization
250 https://www.grid.ac/institutes/grid.412279.b schema:alternateName Universidade de Passo Fundo
251 schema:name Department of Phytopathology, University of Passo Fundo (UPF), BR 285, PO BOX 611, 99052-900, Passo Fundo, RS, Brazil
252 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...