Continuous citric acid fermentation by Candida oleophila under nitrogen limitation at constant C/N ratio View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-07

AUTHORS

S. Anastassiadis, C. Wandrey, H. -J. Rehm

ABSTRACT

The central aspect of this work was to investigate the influence of nitrogen feed rate at constant C/N ratio on continuous citric acid fermentation by Candida oleophila ATCC 20177. Medium ammonia nitrogen and glucose concentrations influenced growth and production. Space-time yield (STY) meaning volumetric productivity, biomass specific productivity (BSP), product concentration, product selectivity and citrate/isocitrate ratio increased with increasing residence time (RT). BSP increased in an exponential mode lowering nitrogen feed rates. Highest BSP for citric acid of 0.13 g/(g h) was achieved at lowest NH4Cl concentration of 1.5 g/l and highest STY (1.2 g/l h) with 3 g NH4Cl/l at a RT of 25 h. Citric acid 74.2 g/l were produced at 58 h RT and 6 g NH4Cl/l. Glucose uptake rate seems to be strictly controlled by growth rate of the yeast cells. Optimum nitrogen concentration and adapted C/N ratio are essential for successful continuous citric acid fermentation. The biomass-specific nitrogen feed rate is the most important factor influencing continuous citric acid production by yeasts. Numerous chemostat experiments showed the feasibility of continuous citrate production by yeasts. More... »

PAGES

695-705

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11274-004-3850-4

DOI

http://dx.doi.org/10.1007/s11274-004-3850-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002387317


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