Ontology type: schema:ScholarlyArticle
2020-05-02
AUTHORSTakuya Nakamura, Jun Inoue, Masashi Ninomiya, Eiji Kakazu, Tomoaki Iwata, Satoshi Takai, Akitoshi Sano, Takayuki Kogure, Tooru Shimosegawa, Atsushi Masamune
ABSTRACTThe frequency of HBV genomic methylation in the liver was reported to vary among patients, but the detailed mechanism is still unknown. In this study, the effects of HBV genome methylation on HBV replication were investigated in vitro. A total of 6 plasmids containing 1.24-fold the HBV genome of different genotypes (subgenotypes A1, A2, B1, and C2) were purified after in vitro methylation with CpG methyltransferase (M.SssI) and transfected into HepG2 cells. In genotype B and C strains, methylation markedly decreased the amount of hepatitis B e antigen (HBeAg) in the culture supernatant. A reduction of hepatitis B surface antigen (HBsAg) was found in some HBV strains but the reduction was smaller than that of HBeAg. There was no significant difference in particle-associated HBV DNA in the culture supernatant. These findings suggest that HBV genomic methylation might be involved in the HBeAg decline in genotype B and C, in part, and that the reduction of HBsAg was less than that of HBeAg. In conclusion, this study showed that the effect of HBV genomic methylation differs among HBV genotypes, suggesting a potential explanation for the different clinical outcomes of genotypes A, B, and C. More... »
PAGES439-447
http://scigraph.springernature.com/pub.10.1007/s11262-020-01761-5
DOIhttp://dx.doi.org/10.1007/s11262-020-01761-5
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1127332403
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/32361814
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Methylation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA, Viral",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genotype",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hep G2 Cells",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hepatitis B e Antigens",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hepatitis B virus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hepatitis B, Chronic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Virus Replication",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Nakamura",
"givenName": "Takuya",
"id": "sg:person.01142073525.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01142073525.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Inoue",
"givenName": "Jun",
"id": "sg:person.01063334634.27",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063334634.27"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Ninomiya",
"givenName": "Masashi",
"id": "sg:person.01135311134.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135311134.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Kakazu",
"givenName": "Eiji",
"id": "sg:person.0704634134.00",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704634134.00"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Iwata",
"givenName": "Tomoaki",
"id": "sg:person.01277711417.63",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277711417.63"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Takai",
"givenName": "Satoshi",
"id": "sg:person.01073760325.48",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073760325.48"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Sano",
"givenName": "Akitoshi",
"id": "sg:person.013245505314.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013245505314.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku Medical and Pharmaceutical University Hospital, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.488554.0",
"name": [
"Division of Gastroenterology, Tohoku Medical and Pharmaceutical University Hospital, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Kogure",
"givenName": "Takayuki",
"id": "sg:person.01362125034.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362125034.62"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Gastroenterology, South Miyagi Medical Center, Ogawara, Japan",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"Department of Gastroenterology, South Miyagi Medical Center, Ogawara, Japan"
],
"type": "Organization"
},
"familyName": "Shimosegawa",
"givenName": "Tooru",
"id": "sg:person.01255535707.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255535707.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan",
"id": "http://www.grid.ac/institutes/grid.69566.3a",
"name": [
"Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai, Japan"
],
"type": "Organization"
},
"familyName": "Masamune",
"givenName": "Atsushi",
"id": "sg:person.01366123207.25",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366123207.25"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/srep10478",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039528451",
"https://doi.org/10.1038/srep10478"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature14192",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010889060",
"https://doi.org/10.1038/nature14192"
],
"type": "CreativeWork"
}
],
"datePublished": "2020-05-02",
"datePublishedReg": "2020-05-02",
"description": "The frequency of HBV genomic methylation in the liver was reported to vary among patients, but the detailed mechanism is still unknown. In this study, the effects of HBV genome methylation on HBV replication were investigated in vitro. A total of 6 plasmids containing 1.24-fold the HBV genome of different genotypes (subgenotypes A1, A2, B1, and C2) were purified after in vitro methylation with CpG methyltransferase (M.SssI) and transfected into HepG2 cells. In genotype B and C strains, methylation markedly decreased the amount of hepatitis B e antigen (HBeAg) in the culture supernatant. A reduction of hepatitis B surface antigen (HBsAg) was found in some HBV strains but the reduction was smaller than that of HBeAg. There was no significant difference in particle-associated HBV DNA in the culture supernatant. These findings suggest that HBV genomic methylation might be involved in the HBeAg decline in genotype B and C, in part, and that the reduction of HBsAg was less than that of HBeAg. In conclusion, this study showed that the effect of HBV genomic methylation differs among HBV genotypes, suggesting a potential explanation for the different clinical outcomes of genotypes A, B, and C.",
"genre": "article",
"id": "sg:pub.10.1007/s11262-020-01761-5",
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.5868261",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.8438780",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1097920",
"issn": [
"0920-8569",
"1572-994X"
],
"name": "Virus Genes",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "56"
}
],
"keywords": [
"genotype B",
"hepatitis B e antigen",
"hepatitis B surface antigen",
"reduction of HBsAg",
"B surface antigen",
"different clinical outcomes",
"hepatitis B virus proteins",
"culture supernatants",
"HBeAg decline",
"HBV replication",
"HBV DNA",
"e antigen",
"HBV strains",
"clinical outcomes",
"HBV genotypes",
"HBV genome",
"surface antigen",
"virus genotypes",
"genotype A",
"c strain",
"HBeAg",
"significant differences",
"HepG2 cells",
"genomic methylation",
"antigen",
"virus proteins",
"viral DNA methylation",
"supernatant",
"genotypes",
"HBsAg",
"different genotypes",
"patients",
"liver",
"DNA methylation",
"potential explanation",
"outcomes",
"methylation",
"total",
"detailed mechanism",
"reduction",
"effect",
"study",
"conclusion",
"cells",
"C. Effects",
"strains",
"expression",
"findings",
"CpG methyltransferase",
"differences",
"decline",
"replication",
"protein",
"genome methylation",
"plasmid",
"mechanism",
"methyltransferase",
"DNA",
"frequency",
"part",
"amount",
"genome",
"explanation"
],
"name": "Effect of viral DNA methylation on expression of hepatitis B virus proteins depends on the virus genotype",
"pagination": "439-447",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1127332403"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11262-020-01761-5"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"32361814"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11262-020-01761-5",
"https://app.dimensions.ai/details/publication/pub.1127332403"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T17:08",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_846.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s11262-020-01761-5"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11262-020-01761-5'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11262-020-01761-5'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11262-020-01761-5'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11262-020-01761-5'
This table displays all metadata directly associated to this object as RDF triples.
242 TRIPLES
21 PREDICATES
99 URIs
89 LITERALS
16 BLANK NODES