Identification of a lytic Pseudomonas aeruginosa phage depolymerase and its anti-biofilm effect and bactericidal contribution to serum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-04-01

AUTHORS

Liyuan Mi, Yannan Liu, Can Wang, Tongtong He, Shan Gao, Shaozhen Xing, Yong Huang, Hang Fan, Xianglilan Zhang, Wengong Yu, Zhiqiang Mi, Yigang Tong, Changqing Bai, Feng Han

ABSTRACT

Pseudomonas aeruginosa (P. aeruginosa) infection has imposed a great threat to patients with cystic fibrosis. With the emergence of multidrug-resistant P. aeruginosa, developing an alternative anti-microbial strategy is indispensable and more urgent than ever. In this study, a lytic P. aeruginosa phage was isolated from the sewage of a hospital, and one protein was predicted as the depolymerase-like protein by genomic sequence analysis, it includes two catalytic regions, the Pectate lyase_3 super family and Glycosyl hydrolase_28 super family. Further analysis demonstrated that recombinant depolymerase-like protein degraded P. aeruginosa exopolysaccharide and enhanced bactericidal activity mediated by serum in vitro. Additionally, this protein disrupted host bacterial biofilms. All of these results showed that the phage-derived depolymerase-like protein has the potential to be developed into an anti-microbial agent that targets P. aeruginosa. More... »

PAGES

1-12

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11262-019-01660-4

DOI

http://dx.doi.org/10.1007/s11262-019-01660-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113170827

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30937696


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