Grapevine virus T is relatively widespread in Slovakia and Czech Republic and genetically diverse View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-10

AUTHORS

Miroslav Glasa, Lukáš Predajňa, Nina Sihelská, Katarína Šoltys, Ana Belén Ruiz-García, Antonio Olmos, Thierry Wetzel, Sead Sabanadzovic

ABSTRACT

A recently described putative foveavirus, grapevine virus T (GVT), was detected in a Slovak grapevine accession (SK704) using high-throughput sequencing, prompting further studies. Full-length genome sequence of isolate GVT-SK704 was determined. Analyses revealed 86.1% nucleotide identity with the Italian GVT isolate, currently the only available nearly complete sequence of GVT in GenBank. A virus-specific RT-PCR assay was developed, which enabled a survey of GVT incidence in grapevine samples from Slovakia and Czech Republic. Unexpectedly, GVT was present in ~ 30% of tested samples. Analysis of complete CP gene sequences of 20 Slovak and Czech GVT isolates detected in the survey revealed relatively high intra-species variability (up to 11.2% nucleotide divergence), suggesting multiple introductions from different sources, possibly over an extended period of time. More... »

PAGES

737-741

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11262-018-1587-7

DOI

http://dx.doi.org/10.1007/s11262-018-1587-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105490484

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29995199


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