Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm View Full Text


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Article Info

DATE

2017-11-15

AUTHORS

Wen-bo Chen, Guo-qing Lu, Hong-mei Cheng, Chen-xi Liu, Yu-tao Xiao, Chao Xu, Zhi-cheng Shen, Mario Soberón, Alejandra Bravo, Kong-ming Wu

ABSTRACT

Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (Helicoverpa armigera) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by H. armigera remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three H. armigera strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric vip3AcAa gene and the cry1Ac gene were introduced into cotton, mortality rates of Cry1Ac resistant H. armigera larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a “pyramid” strategy for pest resistance management in China. More... »

PAGES

763-774

References to SciGraph publications

  • 2012-01-05. Co-expression and Synergism Analysis of Vip3Aa29 and Cyt2Aa3 Insecticidal Proteins from Bacillus thuringiensis in CURRENT MICROBIOLOGY
  • 2003-09. Are Bt crops safe? in NATURE BIOTECHNOLOGY
  • 2012-06-21. Fusing the vegetative insecticidal protein Vip3Aa7 and the N terminus of Cry9Ca improves toxicity against Plutella xylostella larvae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2014-08-26. Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera in SCIENTIFIC REPORTS
  • 2013-06-10. Insect resistance to Bt crops: lessons from the first billion acres in NATURE BIOTECHNOLOGY
  • 2012-06-13. Widespread adoption of Bt cotton and insecticide decrease promotes biocontrol services in NATURE
  • 2016-02-12. Effect of crop plants on fitness costs associated with resistance to Bacillus thuringiensis toxins Cry1Ac and Cry2Ab in cabbage loopers in SCIENTIFIC REPORTS
  • 2013-08-02. No effects of Bacillus thuringiensis maize on nontarget organisms in the field in southern Europe: a meta-analysis of 26 arthropod taxa in TRANSGENIC RESEARCH
  • 2015-01-14. Cross-resistance and interactions between Bt toxins Cry1Ac and Cry2Ab against the cotton bollworm in SCIENTIFIC REPORTS
  • 2016-04-20. Characterization of the resistance to Vip3Aa in Helicoverpa armigera from Australia and the role of midgut processing and receptor binding in SCIENTIFIC REPORTS
  • 2014-11-27. Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm in SCIENTIFIC REPORTS
  • 2014-12-15. Large-scale test of the natural refuge strategy for delaying insect resistance to transgenic Bt crops in NATURE BIOTECHNOLOGY
  • 2012-09-04. Identification of the bioactive core component of the insecticidal Vip3A toxin peptide of Bacillus thuringiensis in JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2015-01-19. Optimizing pyramided transgenic Bt crops for sustainable pest management in NATURE BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-017-0048-8

    DOI

    http://dx.doi.org/10.1007/s11248-017-0048-8

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29143178


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    32 schema:description Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (Helicoverpa armigera) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by H. armigera remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three H. armigera strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric vip3AcAa gene and the cry1Ac gene were introduced into cotton, mortality rates of Cry1Ac resistant H. armigera larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a “pyramid” strategy for pest resistance management in China.
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