Rapid selection of XO embryonic stem cells using Y chromosome-linked GFP transgenic mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10

AUTHORS

Satoshi Yamamoto, Yasumitsu Nagao, Kenji Kuroiwa, Yoji Hakamata, Masaru Ichida, Fumiko Saito-Ohara, Kaoru Tominaga, Hitoshi Endo

ABSTRACT

We developed a transgenic mouse line with Y chromosome-linked green fluorescent protein expressing transgenes (Y-GFP) by the conventional microinjection into the pronucleus of C57BL/6J fertilized oocytes. Embryonic stem (ES) cells derived from Y-GFP mice enabled not only sexing but also the identification of 39, XO karyotype by the lack of Y chromosome. Actually, when fluorescence activated cell sorting (FACS) was applied to Y-GFP ES cells, non-fluorescent ES cells were conveniently collected and showed the lack of Y chromosome by PCR genotyping and Southern blot analysis. FACS analysis revealed Y chromosome loss occurred at 2.9 % of 40, XY ES cells after five passages. These Y-GFP ES cells are potentially applicable to reduce the time, cost and effort needed to generate the gene-targeted mice by the production of male and female mice derived from the same ES cell clone. More... »

PAGES

757-765

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11248-014-9813-0

DOI

http://dx.doi.org/10.1007/s11248-014-9813-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000674776

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25008421


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