Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-02

AUTHORS

Matthias Buntru, Stefanie Gärtner, Lena Staib, Fritz Kreuzaler, Nikolaus Schlaich

ABSTRACT

At present, only few methods for the effective assembly of multigene constructs have been described. Here we present an improved version of the MultiRound Gateway technology, which facilitates plant multigene transformation. The system consists of two attL-flanked entry vectors, which contain an attR cassette, and a transformation-competent artificial chromosome based destination vector. By alternate use of the two entry vectors, multiple transgenes can be delivered sequentially into the Gateway-compatible destination vector. Multigene constructs that carried up to seven transgenes corresponding to more than 26 kb were assembled by seven rounds of LR recombination. The constructs were successfully transformed into tobacco plants and were stably inherited for at least two generations. Thus, our system represents a powerful, highly efficient tool for multigene plant transformation and may facilitate genetic engineering of agronomic traits or the assembly of genetic pathways for the production of biofuels, industrial or pharmaceutical compounds in plants. More... »

PAGES

153-167

References to SciGraph publications

  • 1996-08. Transgene inheritance in plants genetically engineered by microprojectile bombardment in MOLECULAR BIOTECHNOLOGY
  • 1998-11. Fas ligand—a double-edged sword in NATURE BIOTECHNOLOGY
  • 1988-11. Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens in THEORETICAL AND APPLIED GENETICS
  • 2005-10. Transformation of Plants with Multiple Cassettes Generates Simple Transgene Integration Patterns and High Expression Levels in MOLECULAR BREEDING
  • 2006-12. A Gateway-based platform for multigene plant transformation in PLANT MOLECULAR BIOLOGY
  • 2003-12. Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolution in NATURE BIOTECHNOLOGY
  • 2000-03. Agrobacterium-mediated transformation of cereals — from technique development to its application in ACTA PHYSIOLOGIAE PLANTARUM
  • 2007-05. Two NAC domain transcription factors, SND1 and NST1, function redundantly in regulation of secondary wall synthesis in fibers of Arabidopsis in PLANTA
  • 2006-12. From Gateway to MultiSite Gateway in one recombination event in BMC MOLECULAR BIOLOGY
  • 1989-09. A nuclear DNA attachment element mediates elevated and position-independent gene activity in NATURE
  • 1995. Agrobacterium-Mediated Transformation of Rapeseed (Brassica napus) in GENE TRANSFER TO PLANTS
  • 1991-10. A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium in BIO/TECHNOLOGY
  • 2005-03. MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules in NATURE GENETICS
  • 1990-02. Improved binary vectors for Agrobacterium-mediated plant transformation in PLANT MOLECULAR BIOLOGY
  • 1998-11. Expression and inheritance of multiple transgenes in rice plants in NATURE BIOTECHNOLOGY
  • 1984-04. Recombination-dependent recircularization of linearized pBR322 plasmid DNA following transformation of Escherichia coli in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-012-9640-0

    DOI

    http://dx.doi.org/10.1007/s11248-012-9640-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1047416888

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22972476


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Arabidopsis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromosomes, Artificial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tobacco", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transgenes", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Buntru", 
            "givenName": "Matthias", 
            "id": "sg:person.01153535636.87", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153535636.87"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "G\u00e4rtner", 
            "givenName": "Stefanie", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Staib", 
            "givenName": "Lena", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kreuzaler", 
            "givenName": "Fritz", 
            "id": "sg:person.01046656327.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046656327.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RWTH Aachen University", 
              "id": "https://www.grid.ac/institutes/grid.1957.a", 
              "name": [
                "Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schlaich", 
            "givenName": "Nikolaus", 
            "id": "sg:person.01170135415.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170135415.27"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1104/pp.103.027979", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000989298"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11103-006-9065-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004306851", 
              "https://doi.org/10.1007/s11103-006-9065-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jxb/erp006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007874194"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.143000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008344325"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0831166100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008360068"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/3455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008668612", 
              "https://doi.org/10.1038/3455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/3455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008668612", 
              "https://doi.org/10.1038/3455"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.107.106104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008891468"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.111.184374", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012078402"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1091-963", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012182392", 
              "https://doi.org/10.1038/nbt1091-963"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gni035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013266133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.109.152249", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014961838"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.2512204", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015943573"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2199-7-46", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018052364", 
              "https://doi.org/10.1186/1471-2199-7-46"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1505", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019355696", 
              "https://doi.org/10.1038/ng1505"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1505", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019355696", 
              "https://doi.org/10.1038/ng1505"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00018567", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019399815", 
              "https://doi.org/10.1007/bf00018567"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00018567", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019399815", 
              "https://doi.org/10.1007/bf00018567"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0809737105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020420200"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02762320", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022280170", 
              "https://doi.org/10.1007/bf02762320"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tplants.2005.01.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022436371"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-005-0239-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023530783", 
              "https://doi.org/10.1007/s11032-005-0239-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-005-0239-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023530783", 
              "https://doi.org/10.1007/s11032-005-0239-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/341343a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024584701", 
              "https://doi.org/10.1038/341343a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/3511", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026747511", 
              "https://doi.org/10.1038/3511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/3511", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026747511", 
              "https://doi.org/10.1038/3511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0931425100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026971913"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-12-461013-2.50010-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029221085"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00383519", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029239773", 
              "https://doi.org/10.1007/bf00383519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00383519", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029239773", 
              "https://doi.org/10.1007/bf00383519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-79247-2_4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029895603", 
              "https://doi.org/10.1007/978-3-642-79247-2_4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2003.01688.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030282416"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2002.01397.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030835055"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.arplant.48.1.297", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032018806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1987.tb10911.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032308909"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.96.11.6535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032627715"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.tplants.2009.09.010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034832684"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00425-007-0498-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035642322", 
              "https://doi.org/10.1007/s00425-007-0498-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00425-007-0498-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035642322", 
              "https://doi.org/10.1007/s00425-007-0498-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.69.8.2110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038510205"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.00428-07", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038998700"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.88.12.5212", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039397224"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/14.22.8905", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039409376"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-9861(83)90470-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040265310"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.86.21.8467", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040842118"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(87)90584-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041471577"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0968-0004(00)01757-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041734293"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00303524", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044303744", 
              "https://doi.org/10.1007/bf00303524"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2005.02617.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044620157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-313x.2005.02617.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044620157"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/7.6.1513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046335196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2003.10.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046406777"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0003647", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047416429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11738-000-0011-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049918101", 
              "https://doi.org/10.1007/s11738-000-0011-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050439840", 
              "https://doi.org/10.1038/nbt907"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt907", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050439840", 
              "https://doi.org/10.1038/nbt907"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-9452(02)00130-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051378962"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1051/ebr:2008018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056956712"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.287.5450.116", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062567833"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.287.5451.303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062567997"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.17660/actahortic.1998.461.46", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1068396586"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2144/000112536", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069095598"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/187220808784619720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069223707"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2144/01312st04", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074868280"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1990.tb07473.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078765430"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079497685", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2013-02", 
        "datePublishedReg": "2013-02-01", 
        "description": "At present, only few methods for the effective assembly of multigene constructs have been described. Here we present an improved version of the MultiRound Gateway technology, which facilitates plant multigene transformation. The system consists of two attL-flanked entry vectors, which contain an attR cassette, and a transformation-competent artificial chromosome based destination vector. By alternate use of the two entry vectors, multiple transgenes can be delivered sequentially into the Gateway-compatible destination vector. Multigene constructs that carried up to seven transgenes corresponding to more than 26\u00a0kb were assembled by seven rounds of LR recombination. The constructs were successfully transformed into tobacco plants and were stably inherited for at least two generations. Thus, our system represents a powerful, highly efficient tool for multigene plant transformation and may facilitate genetic engineering of agronomic traits or the assembly of genetic pathways for the production of biofuels, industrial or pharmaceutical compounds in plants.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s11248-012-9640-0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1102526", 
            "issn": [
              "0962-8819", 
              "1573-9368"
            ], 
            "name": "Transgenic Research", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "22"
          }
        ], 
        "name": "Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology", 
        "pagination": "153-167", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "e1e0f81713d0561e7c98b48d9d8712ac3e09762240950e446b6a7bba00de5627"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "22972476"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9209120"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11248-012-9640-0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1047416888"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11248-012-9640-0", 
          "https://app.dimensions.ai/details/publication/pub.1047416888"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T01:10", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000524.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs11248-012-9640-0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11248-012-9640-0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11248-012-9640-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11248-012-9640-0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11248-012-9640-0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    317 TRIPLES      21 PREDICATES      95 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11248-012-9640-0 schema:about N1a60d246753a423db19e14e8ac03f547
    2 N2224de9a6d35402a9155d683294260e2
    3 N24169a7b07de4d8fbbab5f3471368884
    4 N45dd69cf7cb649719970263c966f9a64
    5 Nbe2c706250464c128f8bc0d6d4323fba
    6 Nce5de34fd30f42cca99fdf89d8292cfb
    7 Ndeb464bfdf0c4c8494cda87380183365
    8 Ne001789427ff4b8d90d7c74852ec95a5
    9 Ne059c1a37baa41809ad8e173c0e79c61
    10 anzsrc-for:06
    11 anzsrc-for:0607
    12 schema:author N13a1d6a87d3040449d1bb84e7430cc7f
    13 schema:citation sg:pub.10.1007/978-3-642-79247-2_4
    14 sg:pub.10.1007/bf00018567
    15 sg:pub.10.1007/bf00303524
    16 sg:pub.10.1007/bf00383519
    17 sg:pub.10.1007/bf02762320
    18 sg:pub.10.1007/s00425-007-0498-y
    19 sg:pub.10.1007/s11032-005-0239-5
    20 sg:pub.10.1007/s11103-006-9065-3
    21 sg:pub.10.1007/s11738-000-0011-8
    22 sg:pub.10.1038/341343a0
    23 sg:pub.10.1038/3455
    24 sg:pub.10.1038/3511
    25 sg:pub.10.1038/nbt1091-963
    26 sg:pub.10.1038/nbt907
    27 sg:pub.10.1038/ng1505
    28 sg:pub.10.1186/1471-2199-7-46
    29 https://app.dimensions.ai/details/publication/pub.1079497685
    30 https://doi.org/10.1002/j.1460-2075.1990.tb07473.x
    31 https://doi.org/10.1016/0003-9861(83)90470-8
    32 https://doi.org/10.1016/0092-8674(87)90584-8
    33 https://doi.org/10.1016/b978-0-12-461013-2.50010-1
    34 https://doi.org/10.1016/j.jbiotec.2003.10.001
    35 https://doi.org/10.1016/j.tplants.2005.01.008
    36 https://doi.org/10.1016/j.tplants.2009.09.010
    37 https://doi.org/10.1016/s0168-9452(02)00130-9
    38 https://doi.org/10.1016/s0968-0004(00)01757-6
    39 https://doi.org/10.1046/j.1365-313x.2002.01397.x
    40 https://doi.org/10.1046/j.1365-313x.2003.01688.x
    41 https://doi.org/10.1051/ebr:2008018
    42 https://doi.org/10.1073/pnas.0809737105
    43 https://doi.org/10.1073/pnas.0831166100
    44 https://doi.org/10.1073/pnas.0931425100
    45 https://doi.org/10.1073/pnas.69.8.2110
    46 https://doi.org/10.1073/pnas.86.21.8467
    47 https://doi.org/10.1073/pnas.88.12.5212
    48 https://doi.org/10.1073/pnas.96.11.6535
    49 https://doi.org/10.1093/jxb/erp006
    50 https://doi.org/10.1093/nar/14.22.8905
    51 https://doi.org/10.1093/nar/7.6.1513
    52 https://doi.org/10.1093/nar/gni035
    53 https://doi.org/10.1101/gr.143000
    54 https://doi.org/10.1101/gr.2512204
    55 https://doi.org/10.1104/pp.103.027979
    56 https://doi.org/10.1104/pp.107.106104
    57 https://doi.org/10.1104/pp.109.152249
    58 https://doi.org/10.1104/pp.111.184374
    59 https://doi.org/10.1111/j.1365-313x.2005.02617.x
    60 https://doi.org/10.1111/j.1432-1033.1987.tb10911.x
    61 https://doi.org/10.1126/science.287.5450.116
    62 https://doi.org/10.1126/science.287.5451.303
    63 https://doi.org/10.1128/jb.00428-07
    64 https://doi.org/10.1146/annurev.arplant.48.1.297
    65 https://doi.org/10.1371/journal.pone.0003647
    66 https://doi.org/10.17660/actahortic.1998.461.46
    67 https://doi.org/10.2144/000112536
    68 https://doi.org/10.2144/01312st04
    69 https://doi.org/10.2174/187220808784619720
    70 schema:datePublished 2013-02
    71 schema:datePublishedReg 2013-02-01
    72 schema:description At present, only few methods for the effective assembly of multigene constructs have been described. Here we present an improved version of the MultiRound Gateway technology, which facilitates plant multigene transformation. The system consists of two attL-flanked entry vectors, which contain an attR cassette, and a transformation-competent artificial chromosome based destination vector. By alternate use of the two entry vectors, multiple transgenes can be delivered sequentially into the Gateway-compatible destination vector. Multigene constructs that carried up to seven transgenes corresponding to more than 26 kb were assembled by seven rounds of LR recombination. The constructs were successfully transformed into tobacco plants and were stably inherited for at least two generations. Thus, our system represents a powerful, highly efficient tool for multigene plant transformation and may facilitate genetic engineering of agronomic traits or the assembly of genetic pathways for the production of biofuels, industrial or pharmaceutical compounds in plants.
    73 schema:genre research_article
    74 schema:inLanguage en
    75 schema:isAccessibleForFree false
    76 schema:isPartOf Na757a57d9c584bbe9b60a155f2122b82
    77 Nd47e23c3fe76477aa21c84952a936d4a
    78 sg:journal.1102526
    79 schema:name Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology
    80 schema:pagination 153-167
    81 schema:productId N2e0c293a508f49db892fdf670a722e54
    82 N37ed72f01c4341cdaca3e93f26f1444f
    83 Nbfec1a83cdb846f3978ce0ec98ed60bc
    84 Nd1e6cad32b7a46c9a693b699cb70755b
    85 Ne0ec12a542c447c9afe8aeabdd7569d4
    86 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047416888
    87 https://doi.org/10.1007/s11248-012-9640-0
    88 schema:sdDatePublished 2019-04-11T01:10
    89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    90 schema:sdPublisher N9d85e39392d14be28d2aa405991139bc
    91 schema:url http://link.springer.com/10.1007%2Fs11248-012-9640-0
    92 sgo:license sg:explorer/license/
    93 sgo:sdDataset articles
    94 rdf:type schema:ScholarlyArticle
    95 N13a1d6a87d3040449d1bb84e7430cc7f rdf:first sg:person.01153535636.87
    96 rdf:rest Nd1db6d2e2c2d4e529c3aaad6e0cc174f
    97 N1a60d246753a423db19e14e8ac03f547 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Transgenes
    99 rdf:type schema:DefinedTerm
    100 N2224de9a6d35402a9155d683294260e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Genetic Engineering
    102 rdf:type schema:DefinedTerm
    103 N24169a7b07de4d8fbbab5f3471368884 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Cloning, Molecular
    105 rdf:type schema:DefinedTerm
    106 N2e0c293a508f49db892fdf670a722e54 schema:name dimensions_id
    107 schema:value pub.1047416888
    108 rdf:type schema:PropertyValue
    109 N37ed72f01c4341cdaca3e93f26f1444f schema:name doi
    110 schema:value 10.1007/s11248-012-9640-0
    111 rdf:type schema:PropertyValue
    112 N43239d866cad446d94c0d786e055b989 rdf:first Ne74fa958bbe5431b8c0023a72d0c1e2b
    113 rdf:rest N900cb4c70bfa4455979077491e842717
    114 N45dd69cf7cb649719970263c966f9a64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Plants, Genetically Modified
    116 rdf:type schema:DefinedTerm
    117 N6bbc177d6d2948ed97f8ba755c9894fc schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    118 schema:familyName Gärtner
    119 schema:givenName Stefanie
    120 rdf:type schema:Person
    121 N88c4e87d80a9444ab8a54a44c3350adf rdf:first sg:person.01170135415.27
    122 rdf:rest rdf:nil
    123 N900cb4c70bfa4455979077491e842717 rdf:first sg:person.01046656327.32
    124 rdf:rest N88c4e87d80a9444ab8a54a44c3350adf
    125 N9d85e39392d14be28d2aa405991139bc schema:name Springer Nature - SN SciGraph project
    126 rdf:type schema:Organization
    127 Na757a57d9c584bbe9b60a155f2122b82 schema:volumeNumber 22
    128 rdf:type schema:PublicationVolume
    129 Nbe2c706250464c128f8bc0d6d4323fba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Chromosomes, Artificial
    131 rdf:type schema:DefinedTerm
    132 Nbfec1a83cdb846f3978ce0ec98ed60bc schema:name nlm_unique_id
    133 schema:value 9209120
    134 rdf:type schema:PropertyValue
    135 Nce5de34fd30f42cca99fdf89d8292cfb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Gene Transfer Techniques
    137 rdf:type schema:DefinedTerm
    138 Nd1db6d2e2c2d4e529c3aaad6e0cc174f rdf:first N6bbc177d6d2948ed97f8ba755c9894fc
    139 rdf:rest N43239d866cad446d94c0d786e055b989
    140 Nd1e6cad32b7a46c9a693b699cb70755b schema:name pubmed_id
    141 schema:value 22972476
    142 rdf:type schema:PropertyValue
    143 Nd47e23c3fe76477aa21c84952a936d4a schema:issueNumber 1
    144 rdf:type schema:PublicationIssue
    145 Ndeb464bfdf0c4c8494cda87380183365 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Tobacco
    147 rdf:type schema:DefinedTerm
    148 Ne001789427ff4b8d90d7c74852ec95a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Arabidopsis
    150 rdf:type schema:DefinedTerm
    151 Ne059c1a37baa41809ad8e173c0e79c61 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Genetic Vectors
    153 rdf:type schema:DefinedTerm
    154 Ne0ec12a542c447c9afe8aeabdd7569d4 schema:name readcube_id
    155 schema:value e1e0f81713d0561e7c98b48d9d8712ac3e09762240950e446b6a7bba00de5627
    156 rdf:type schema:PropertyValue
    157 Ne74fa958bbe5431b8c0023a72d0c1e2b schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    158 schema:familyName Staib
    159 schema:givenName Lena
    160 rdf:type schema:Person
    161 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    162 schema:name Biological Sciences
    163 rdf:type schema:DefinedTerm
    164 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    165 schema:name Plant Biology
    166 rdf:type schema:DefinedTerm
    167 sg:journal.1102526 schema:issn 0962-8819
    168 1573-9368
    169 schema:name Transgenic Research
    170 rdf:type schema:Periodical
    171 sg:person.01046656327.32 schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    172 schema:familyName Kreuzaler
    173 schema:givenName Fritz
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01046656327.32
    175 rdf:type schema:Person
    176 sg:person.01153535636.87 schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    177 schema:familyName Buntru
    178 schema:givenName Matthias
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153535636.87
    180 rdf:type schema:Person
    181 sg:person.01170135415.27 schema:affiliation https://www.grid.ac/institutes/grid.1957.a
    182 schema:familyName Schlaich
    183 schema:givenName Nikolaus
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170135415.27
    185 rdf:type schema:Person
    186 sg:pub.10.1007/978-3-642-79247-2_4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029895603
    187 https://doi.org/10.1007/978-3-642-79247-2_4
    188 rdf:type schema:CreativeWork
    189 sg:pub.10.1007/bf00018567 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019399815
    190 https://doi.org/10.1007/bf00018567
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.1007/bf00303524 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044303744
    193 https://doi.org/10.1007/bf00303524
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1007/bf00383519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029239773
    196 https://doi.org/10.1007/bf00383519
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1007/bf02762320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022280170
    199 https://doi.org/10.1007/bf02762320
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1007/s00425-007-0498-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1035642322
    202 https://doi.org/10.1007/s00425-007-0498-y
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1007/s11032-005-0239-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023530783
    205 https://doi.org/10.1007/s11032-005-0239-5
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1007/s11103-006-9065-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004306851
    208 https://doi.org/10.1007/s11103-006-9065-3
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1007/s11738-000-0011-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049918101
    211 https://doi.org/10.1007/s11738-000-0011-8
    212 rdf:type schema:CreativeWork
    213 sg:pub.10.1038/341343a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024584701
    214 https://doi.org/10.1038/341343a0
    215 rdf:type schema:CreativeWork
    216 sg:pub.10.1038/3455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008668612
    217 https://doi.org/10.1038/3455
    218 rdf:type schema:CreativeWork
    219 sg:pub.10.1038/3511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026747511
    220 https://doi.org/10.1038/3511
    221 rdf:type schema:CreativeWork
    222 sg:pub.10.1038/nbt1091-963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012182392
    223 https://doi.org/10.1038/nbt1091-963
    224 rdf:type schema:CreativeWork
    225 sg:pub.10.1038/nbt907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050439840
    226 https://doi.org/10.1038/nbt907
    227 rdf:type schema:CreativeWork
    228 sg:pub.10.1038/ng1505 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019355696
    229 https://doi.org/10.1038/ng1505
    230 rdf:type schema:CreativeWork
    231 sg:pub.10.1186/1471-2199-7-46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018052364
    232 https://doi.org/10.1186/1471-2199-7-46
    233 rdf:type schema:CreativeWork
    234 https://app.dimensions.ai/details/publication/pub.1079497685 schema:CreativeWork
    235 https://doi.org/10.1002/j.1460-2075.1990.tb07473.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078765430
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/0003-9861(83)90470-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040265310
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1016/0092-8674(87)90584-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041471577
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/b978-0-12-461013-2.50010-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029221085
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1016/j.jbiotec.2003.10.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046406777
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1016/j.tplants.2005.01.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022436371
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1016/j.tplants.2009.09.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034832684
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1016/s0168-9452(02)00130-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051378962
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1016/s0968-0004(00)01757-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041734293
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1046/j.1365-313x.2002.01397.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030835055
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1046/j.1365-313x.2003.01688.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030282416
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1051/ebr:2008018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056956712
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1073/pnas.0809737105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020420200
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1073/pnas.0831166100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008360068
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1073/pnas.0931425100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026971913
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1073/pnas.69.8.2110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038510205
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1073/pnas.86.21.8467 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040842118
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1073/pnas.88.12.5212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039397224
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1073/pnas.96.11.6535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032627715
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1093/jxb/erp006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007874194
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1093/nar/14.22.8905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039409376
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1093/nar/7.6.1513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046335196
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1093/nar/gni035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013266133
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1101/gr.143000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008344325
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1101/gr.2512204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015943573
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1104/pp.103.027979 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000989298
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1104/pp.107.106104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008891468
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1104/pp.109.152249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014961838
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1104/pp.111.184374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012078402
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1111/j.1365-313x.2005.02617.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044620157
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1111/j.1432-1033.1987.tb10911.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032308909
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1126/science.287.5450.116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062567833
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1126/science.287.5451.303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062567997
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1128/jb.00428-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038998700
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1146/annurev.arplant.48.1.297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032018806
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1371/journal.pone.0003647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047416429
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.17660/actahortic.1998.461.46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068396586
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.2144/000112536 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095598
    310 rdf:type schema:CreativeWork
    311 https://doi.org/10.2144/01312st04 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074868280
    312 rdf:type schema:CreativeWork
    313 https://doi.org/10.2174/187220808784619720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069223707
    314 rdf:type schema:CreativeWork
    315 https://www.grid.ac/institutes/grid.1957.a schema:alternateName RWTH Aachen University
    316 schema:name Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany
    317 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...