Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-02

AUTHORS

Krisztián Kvell, Tamás Czömpöly, László Hiripi, Péter Balogh, József Kóbor, Lilla Bodrogi, Judit E. Pongrácz, William A. Ritchie, Zsuzsanna Bősze

ABSTRACT

Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1alpha promoter. The established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry. eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were observed. Up to the sixth generation only one newborn showed eGFP inactivation. eGFP + transgenic bone marrow cells efficiently provided long-term haemopoietic repopulation in radiation chimeras, regenerating all bone marrow-derived lineages with eGFP + cells with distinct eGFP expression profiles. The established eGFP + BALB/c mouse strain is expected to be extremely useful in various immunological experiments. More... »

PAGES

105-112

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11248-009-9288-6

DOI

http://dx.doi.org/10.1007/s11248-009-9288-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026351443

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19484500


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