Different subcellular localization and glycosylation for a functional antibody expressed in Nicotiana tabacum plants and suspension cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-06

AUTHORS

Benoit De Muynck, Catherine Navarre, Yannick Nizet, Johannes Stadlmann, Marc Boutry

ABSTRACT

Genes encoding the heavy and light chains of LO-BM2, a therapeutic IgG antibody, were assembled in the tandem or inverted convergent orientation and expressed in Nicotiana tabacum plants and BY-2 suspension cells. The tandem construct allowed higher expression in both expression systems. A similar degradation pattern was observed for the secreted antibody recovered from the leaf intercellular fluid and BY-2 culture medium. Degradation increased with leaf age or culture time. Antibodies purified from leaf tissues and BY-2 cells were both functional. However, MS analysis of the N-glycosylation showed complex plant-type glycans to be the major type in the antibody purified from plants, whereas, oligomannosidic was the major glycosylation type in that purified from BY-2 cells. LO-BM2 was observed mainly in the endoplasmic reticulum of BY-2 cells while, in leaf cells, it was localized mostly to vesicles resembling prevacuolar compartments. These results and those from endoglycosidase H studies suggest that LO-BM2 is secreted from BY-2 cells more readily than from leaf cells where it accumulates in a post-Golgi compartment. More... »

PAGES

467-482

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-008-9240-1

    DOI

    http://dx.doi.org/10.1007/s11248-008-9240-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003098493

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19140023


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