Improved plant transformation vectors for fluorescent protein tagging View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-10

AUTHORS

Silin Zhong, Zhefeng Lin, Rupert G. Fray, Don Grierson

ABSTRACT

Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a series of 23 novel GATEWAY-compatible vectors based on pGreenII and pDH51 backbones with the latest fluorescent protein tags (Cerulean, EGFP and Venus) and the choice of three in planta selection markers. These vectors can be obtained from the Nottingham Arabidopsis Stock Centre (N9819-N9846) and should be a powerful tool box for transgenic research in plants. More... »

PAGES

985-989

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11248-008-9199-y

DOI

http://dx.doi.org/10.1007/s11248-008-9199-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037017928

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18594998


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