Cytokinin vectors mediate marker-free and backbone-free plant transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-10

AUTHORS

Craig M. Richael, Marina Kalyaeva, Robert C. Chretien, Hua Yan, Sathya Adimulam, Artesia Stivison, J. Troy Weeks, Caius M. Rommens

ABSTRACT

Conventional Agrobacterium-mediated transformation methods rely on complex and genotype-specific tissue culture media for selection, proliferation, and regeneration of genetically modified cells. Resulting transgenic plants may not only contain selectable marker genes but also carry fragments of the vector backbone. Here, we describe a new method for the production of transgenic plants that lack such foreign DNA. This method employs vectors containing the bacterial isopentenyltransferase (ipt) gene as backbone integration marker. Agrobacterium strains carrying the resulting ipt gene-containing "cytokinin" vectors were used to infect explants of various Solanaceous plant species as well as canola (Brassica napus). Upon transfer to hormone-free media, 1.8% to 9.9% of the infected explants produced shoots that contained a marker-free T-DNA while lacking the backbone integration marker. These frequencies often equal or exceed those for backbone-free conventional transformation. More... »

PAGES

905-917

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11248-008-9175-6

DOI

http://dx.doi.org/10.1007/s11248-008-9175-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034018763

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18320338


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