Development of an in planta method for transformation of alfalfa (Medicago sativa) View Full Text


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Article Info

DATE

2008-08

AUTHORS

J Troy Weeks, Jingsong Ye, Caius M Rommens

ABSTRACT

Conventional methods in transforming alfalfa (Medicago sativa) require multiple tissue culture manipulations that are time-consuming and expensive, while applicable only to a few highly regenerable genotypes. Here, we describe a simple in planta method that makes it possible to transform a commercial variety without employing selectable marker genes. Basically, young seedlings are cut at the apical node, cold-treated, and vigorously vortexed in an Agrobacterium suspension also containing sand. About 7% of treated seedlings produced progenies segregating for the T-DNA. The vortex-mediated seedling transformation method was applied to transform alfalfa with an all-native transfer DNA comprising a silencing construct for the caffeic acid o-methyltransferase (Comt) gene. Resulting intragenic plants accumulated reduced levels of the indigestible fiber component lignin that lowers forage quality. The absence of both selectable marker genes and other foreign genetic elements may expedite the governmental approval process for quality-enhanced alfalfa. More... »

PAGES

587-597

References to SciGraph publications

  • 2007-04. US courts thwart GM alfalfa and turf grass in NATURE BIOTECHNOLOGY
  • 2003-11. A framework for designing transgenic crops—science, safety and citizen's concerns in NATURE BIOTECHNOLOGY
  • 2001-02. Chemical-regulated, site-specific DNA excision in transgenic plants in NATURE BIOTECHNOLOGY
  • 2001-08. Transgenic radish (Raphanus sativus L. longipinnatus Bailey) by floral-dip method – plant development and surfactant are important in optimizing transformation efficiency in TRANSGENIC RESEARCH
  • 2006-06-01. <i>Arabidopsis thaliana</i> Floral Dip Transformation Method in AGROBACTERIUM PROTOCOLS
  • 2001-06. Germ-line transformation of Arabidopsis lasiocarpa in TRANSGENIC RESEARCH
  • 2006-06-01. Alfalfa (<i>Medicago sativa</i> L.) in AGROBACTERIUM PROTOCOLS
  • 2001-10. Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferases in TRANSGENIC RESEARCH
  • 2003-04. A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop in NATURE BIOTECHNOLOGY
  • 1997-09. SAAT: sonication-assisted Agrobacterium-mediated transformation in TRANSGENIC RESEARCH
  • 1992-02. Transactivation of Ds by Ac-transposase gene fusions in tobacco in MOLECULAR GENETICS AND GENOMICS
  • 2002-08. High Efficiency Transgene Segregation in Co-Transformed Maize Plants using an Agrobacterium Tumefaciens 2 T-DNA Binary System in TRANSGENIC RESEARCH
  • 1992-01. Somaclonal variation in winter wheat: frequency, occurrence and inheritance in EUPHYTICA
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-007-9132-9

    DOI

    http://dx.doi.org/10.1007/s11248-007-9132-9

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17851774


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