Improvement of Human lysozyme Expression in Transgenic Rice Grain by Combining Wheat (Triticum aestivum) puroindoline b and Rice (Oryza sativa) ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-10

AUTHORS

Kevin Hennegan, Daichang Yang, Diane Nguyen, Liying Wu, Jeff Goding, Jianmin Huang, Fengli Guo, Ning Huang, Simon C. Watkins

ABSTRACT

Heterologous protein expression levels in transgenic plants are of critical importance in the production of plant-made pharmaceuticals (PMPs). We studied a puroindoline b promoter and signal peptide (Tapur) driving human lysozyme expression in rice endosperm. The results demonstrated that human lysozyme expressed under the control of the Tapur cassette is seed-specific, readily extractable, active, and properly processed. Immuno-electron microscopy indicated that lysozyme expressed from this cassette is localized in protein bodies I and II in rice endosperm cells, demonstrating that this non-storage promoter and signal peptide can be used for targeting human lysozyme to rice protein bodies. We successfully employed a strategy to improve the expression of human lysozyme in transgenic rice grain by combining the Tapur cassette with our well established Gt1 expression system. The results demonstrated that when the two expression cassettes were combined, the expression level of human lysozyme increased from 5.24 +/- 0.34 mg(-1) g flour for the best single cassette line to 9.24 +/- 0.06 mg(-1) g flour in the best double cassette line, indicating an additive effect on expression of human lysozyme in rice grain. More... »

PAGES

583-592

References to SciGraph publications

  • 2000-06. Elevation of the provitamin A content of transgenic tomato plants in NATURE BIOTECHNOLOGY
  • 1998-09. Sorting of proteins to vacuoles in plant cells in PLANT MOLECULAR BIOLOGY
  • 2002-09. Expression of natural antimicrobial human lysozyme in rice grains in MOLECULAR BREEDING
  • 2000-03. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies in PLANT MOLECULAR BIOLOGY
  • 1997-08. Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification in MOLECULAR BREEDING
  • 2001-02. Expression of wheat puroindoline genes in transgenic rice enhances grain softness in NATURE BIOTECHNOLOGY
  • 2003-02. Expression and localization of human lysozyme in the endosperm of transgenic rice in PLANTA
  • 2002-02. Analysis of the rice endosperm-specific globulin promoter in transformed rice cells in PLANT CELL REPORTS
  • 2001-12. Field assessments of gene flow from transgenic to cultivated rice (Oryza sativa L.) using a herbicide resistance gene as tracer marker in THEORETICAL AND APPLIED GENETICS
  • 1998-09. Deposition of storage proteins in PLANT MOLECULAR BIOLOGY
  • 1999-04. Cloning of a wheat puroindoline gene promoter by IPCR and analysis of promoter regions required for tissue-specific expression in transgenic rice seeds in PLANT MOLECULAR BIOLOGY
  • 2000-02. Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants in MOLECULAR BREEDING
  • 2000-02. Expression of full-length bioactive antimicrobial human lactoferrin in potato plants in TRANSGENIC RESEARCH
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-004-6702-y

    DOI

    http://dx.doi.org/10.1007/s11248-004-6702-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012528307

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16245149


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Recombinant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Profiling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Glutens", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "In Vitro Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Muramidase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oryza", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Sorting Signals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tissue Distribution", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Triticum", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hennegan", 
            "givenName": "Kevin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Daichang", 
            "id": "sg:person.01136175674.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136175674.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nguyen", 
            "givenName": "Diane", 
            "id": "sg:person.01047130531.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047130531.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA", 
                "Arcadia Bioscience, 202 Cousteau Ct., 95616, Davis, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wu", 
            "givenName": "Liying", 
            "id": "sg:person.01230730044.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230730044.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Goding", 
            "givenName": "Jeff", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huang", 
            "givenName": "Jianmin", 
            "id": "sg:person.0757010643.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757010643.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Pittsburgh", 
              "id": "https://www.grid.ac/institutes/grid.21925.3d", 
              "name": [
                "Department of Cell Biology and Physiology, University of Pittsburgh, 15261, PA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guo", 
            "givenName": "Fengli", 
            "id": "sg:person.01006262567.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006262567.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huang", 
            "givenName": "Ning", 
            "id": "sg:person.01300502704.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300502704.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Pittsburgh", 
              "id": "https://www.grid.ac/institutes/grid.21925.3d", 
              "name": [
                "Department of Cell Biology and Physiology, University of Pittsburgh, 15261, PA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Watkins", 
            "givenName": "Simon C.", 
            "id": "sg:person.014011023502.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014011023502.91"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/84435", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000490996", 
              "https://doi.org/10.1038/84435"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/84435", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000490996", 
              "https://doi.org/10.1038/84435"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008977630179", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003922442", 
              "https://doi.org/10.1023/a:1008977630179"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-9452(02)00165-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005188956"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(99)01389-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008859318"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0006-3002(52)90045-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016042812"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0006-3002(52)90045-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016042812"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1009648532210", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018305891", 
              "https://doi.org/10.1023/a:1009648532210"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1006301519427", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019195519", 
              "https://doi.org/10.1023/a:1006301519427"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1105/tpc.7.7.945", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020002066"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.97.25.13512", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023326528"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001220100713", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027805331", 
              "https://doi.org/10.1007/s001220100713"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s001220100713", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027805331", 
              "https://doi.org/10.1007/s001220100713"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1020355511981", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028519364", 
              "https://doi.org/10.1023/a:1020355511981"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.201411298", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030999866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.030527497", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033144170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1467-7652.2003.00038.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034221632"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1006032627036", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034710676", 
              "https://doi.org/10.1023/a:1006032627036"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-9452(98)00121-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035403433"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2003.01636.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037655632"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1009676322162", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038951188", 
              "https://doi.org/10.1023/a:1009676322162"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1006194326804", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040809660", 
              "https://doi.org/10.1023/a:1006194326804"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-9452(01)00447-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043773491"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00425-002-0919-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047760803", 
              "https://doi.org/10.1007/s00425-002-0919-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-001-0406-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048483169", 
              "https://doi.org/10.1007/s00299-001-0406-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-001-0406-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048483169", 
              "https://doi.org/10.1007/s00299-001-0406-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1006020208380", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050613832", 
              "https://doi.org/10.1023/a:1006020208380"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1836901100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050644461"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/76523", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050784753", 
              "https://doi.org/10.1038/76523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/76523", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050784753", 
              "https://doi.org/10.1038/76523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(93)90576-c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052790323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi.2001.14.10.1255", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060080314"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2135/cropsci2002.0278", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069027450"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/3870049", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1070467989"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2135/cropsci2002.2780", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090703518"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2005-10", 
        "datePublishedReg": "2005-10-01", 
        "description": "Heterologous protein expression levels in transgenic plants are of critical importance in the production of plant-made pharmaceuticals (PMPs). We studied a puroindoline b promoter and signal peptide (Tapur) driving human lysozyme expression in rice endosperm. The results demonstrated that human lysozyme expressed under the control of the Tapur cassette is seed-specific, readily extractable, active, and properly processed. Immuno-electron microscopy indicated that lysozyme expressed from this cassette is localized in protein bodies I and II in rice endosperm cells, demonstrating that this non-storage promoter and signal peptide can be used for targeting human lysozyme to rice protein bodies. We successfully employed a strategy to improve the expression of human lysozyme in transgenic rice grain by combining the Tapur cassette with our well established Gt1 expression system. The results demonstrated that when the two expression cassettes were combined, the expression level of human lysozyme increased from 5.24 +/- 0.34 mg(-1) g flour for the best single cassette line to 9.24 +/- 0.06 mg(-1) g flour in the best double cassette line, indicating an additive effect on expression of human lysozyme in rice grain.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s11248-004-6702-y", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1102526", 
            "issn": [
              "0962-8819", 
              "1573-9368"
            ], 
            "name": "Transgenic Research", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "14"
          }
        ], 
        "name": "Improvement of Human lysozyme Expression in Transgenic Rice Grain by Combining Wheat (Triticum aestivum) puroindoline b and Rice (Oryza sativa) Gt1 Promoters and Signal Peptides", 
        "pagination": "583-592", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "3bb0269183d0b65039fb9316edc766bd2b06b333b41a57c0f476e159f84526ed"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16245149"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9209120"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11248-004-6702-y"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1012528307"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11248-004-6702-y", 
          "https://app.dimensions.ai/details/publication/pub.1012528307"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:20", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87078_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs11248-004-6702-y"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11248-004-6702-y'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11248-004-6702-y'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11248-004-6702-y'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11248-004-6702-y'


     

    This table displays all metadata directly associated to this object as RDF triples.

    313 TRIPLES      21 PREDICATES      77 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11248-004-6702-y schema:about N11599c01f5e24caeb730d4b256cd6f0b
    2 N1a32e910f8e24ad49cdeeed03046d8b0
    3 N1ba4bba36c30493a954e7e573a603ef4
    4 N257d5b618e6b4c2f884691746afb1c5c
    5 N2866682c58f14d49b69c52354a42d13a
    6 N31d22e668e1d4d2e84f83aa47b584601
    7 N331df31946de46708901fff8264f2560
    8 N49fabade71dc48a491dd54e215e0ed3b
    9 N4c36224be348410c91154b32f562cd65
    10 N57dddbf4cd20420c8f5bbf651cec3abe
    11 N5a531ee157fd483a928b10c79cf9641e
    12 N68620affb3fb4008ab974adb933a8c77
    13 N881cbd39b5cb414c902b0b797b8bed5d
    14 Nb0fe4b9172e54e3fb8a788ab667e63ac
    15 Nc098a9846ca54702bc4a8d31898718f5
    16 Ne640779d6e6a4d81bc8d21734d767c60
    17 Nf2af36d6cf904c0ab0aa4543389ad8e7
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author Ne889cead3487463cba6ef4f1d983d871
    21 schema:citation sg:pub.10.1007/bf02712670
    22 sg:pub.10.1007/s001220100713
    23 sg:pub.10.1007/s00299-001-0406-z
    24 sg:pub.10.1007/s00425-002-0919-x
    25 sg:pub.10.1023/a:1006020208380
    26 sg:pub.10.1023/a:1006032627036
    27 sg:pub.10.1023/a:1006194326804
    28 sg:pub.10.1023/a:1006301519427
    29 sg:pub.10.1023/a:1008977630179
    30 sg:pub.10.1023/a:1009648532210
    31 sg:pub.10.1023/a:1009676322162
    32 sg:pub.10.1023/a:1020355511981
    33 sg:pub.10.1038/76523
    34 sg:pub.10.1038/84435
    35 https://doi.org/10.1016/0006-3002(52)90045-0
    36 https://doi.org/10.1016/0092-8674(93)90576-c
    37 https://doi.org/10.1016/s0168-9452(01)00447-2
    38 https://doi.org/10.1016/s0168-9452(02)00165-6
    39 https://doi.org/10.1016/s0168-9452(98)00121-6
    40 https://doi.org/10.1016/s1360-1385(99)01389-8
    41 https://doi.org/10.1046/j.1365-313x.2003.01636.x
    42 https://doi.org/10.1046/j.1467-7652.2003.00038.x
    43 https://doi.org/10.1073/pnas.030527497
    44 https://doi.org/10.1073/pnas.1836901100
    45 https://doi.org/10.1073/pnas.201411298
    46 https://doi.org/10.1073/pnas.97.25.13512
    47 https://doi.org/10.1094/mpmi.2001.14.10.1255
    48 https://doi.org/10.1105/tpc.7.7.945
    49 https://doi.org/10.2135/cropsci2002.0278
    50 https://doi.org/10.2135/cropsci2002.2780
    51 https://doi.org/10.2307/3870049
    52 schema:datePublished 2005-10
    53 schema:datePublishedReg 2005-10-01
    54 schema:description Heterologous protein expression levels in transgenic plants are of critical importance in the production of plant-made pharmaceuticals (PMPs). We studied a puroindoline b promoter and signal peptide (Tapur) driving human lysozyme expression in rice endosperm. The results demonstrated that human lysozyme expressed under the control of the Tapur cassette is seed-specific, readily extractable, active, and properly processed. Immuno-electron microscopy indicated that lysozyme expressed from this cassette is localized in protein bodies I and II in rice endosperm cells, demonstrating that this non-storage promoter and signal peptide can be used for targeting human lysozyme to rice protein bodies. We successfully employed a strategy to improve the expression of human lysozyme in transgenic rice grain by combining the Tapur cassette with our well established Gt1 expression system. The results demonstrated that when the two expression cassettes were combined, the expression level of human lysozyme increased from 5.24 +/- 0.34 mg(-1) g flour for the best single cassette line to 9.24 +/- 0.06 mg(-1) g flour in the best double cassette line, indicating an additive effect on expression of human lysozyme in rice grain.
    55 schema:genre research_article
    56 schema:inLanguage en
    57 schema:isAccessibleForFree false
    58 schema:isPartOf N0ec3be1d0c2140549a899815277fd93a
    59 Nd41152587011423e9c960f1766cf68b8
    60 sg:journal.1102526
    61 schema:name Improvement of Human lysozyme Expression in Transgenic Rice Grain by Combining Wheat (Triticum aestivum) puroindoline b and Rice (Oryza sativa) Gt1 Promoters and Signal Peptides
    62 schema:pagination 583-592
    63 schema:productId N0c53d464f61a49e89122579d6ba5bd11
    64 N0e9d4f95145c49249394dd360a5a86bb
    65 N60e2c13480b04dab9c00bb7b6d0eee47
    66 Na463b9d7d80f4b2aaf3554da34e69f33
    67 Nf8e025180eec48ef8c9f8f607e672ea0
    68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012528307
    69 https://doi.org/10.1007/s11248-004-6702-y
    70 schema:sdDatePublished 2019-04-11T12:20
    71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    72 schema:sdPublisher N8506e7f6f87849b1aca00ee586930ab9
    73 schema:url http://link.springer.com/10.1007%2Fs11248-004-6702-y
    74 sgo:license sg:explorer/license/
    75 sgo:sdDataset articles
    76 rdf:type schema:ScholarlyArticle
    77 N07be7c7d52cd4d0494430b9d31d3b8ae rdf:first sg:person.01136175674.75
    78 rdf:rest N0b023c7dc04f4d909e3f4983924fad2a
    79 N0b023c7dc04f4d909e3f4983924fad2a rdf:first sg:person.01047130531.26
    80 rdf:rest N4c48ca80660049a28fa5475ecd04770c
    81 N0c53d464f61a49e89122579d6ba5bd11 schema:name pubmed_id
    82 schema:value 16245149
    83 rdf:type schema:PropertyValue
    84 N0e9d4f95145c49249394dd360a5a86bb schema:name doi
    85 schema:value 10.1007/s11248-004-6702-y
    86 rdf:type schema:PropertyValue
    87 N0ec3be1d0c2140549a899815277fd93a schema:volumeNumber 14
    88 rdf:type schema:PublicationVolume
    89 N11599c01f5e24caeb730d4b256cd6f0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Promoter Regions, Genetic
    91 rdf:type schema:DefinedTerm
    92 N1478b9de988d40f09de26b523fbe2012 schema:name Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    93 rdf:type schema:Organization
    94 N1747ac7a340842e292efa5bb66f37ec6 schema:name Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    95 rdf:type schema:Organization
    96 N1a32e910f8e24ad49cdeeed03046d8b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Muramidase
    98 rdf:type schema:DefinedTerm
    99 N1b46c22f54db4f19b49dfe7e72da5e10 rdf:first sg:person.0757010643.55
    100 rdf:rest N9cccfd00ef854e81a8dbb4aba925d062
    101 N1ba4bba36c30493a954e7e573a603ef4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Plant Proteins
    103 rdf:type schema:DefinedTerm
    104 N1d04960daea1425da81fe00659957681 rdf:first Nc8d19c6a89fd43c584e7557c435dfd26
    105 rdf:rest N1b46c22f54db4f19b49dfe7e72da5e10
    106 N257d5b618e6b4c2f884691746afb1c5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Tissue Distribution
    108 rdf:type schema:DefinedTerm
    109 N2866682c58f14d49b69c52354a42d13a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Plants, Genetically Modified
    111 rdf:type schema:DefinedTerm
    112 N31d22e668e1d4d2e84f83aa47b584601 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name DNA, Recombinant
    114 rdf:type schema:DefinedTerm
    115 N331df31946de46708901fff8264f2560 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Humans
    117 rdf:type schema:DefinedTerm
    118 N49fabade71dc48a491dd54e215e0ed3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Plasmids
    120 rdf:type schema:DefinedTerm
    121 N4c36224be348410c91154b32f562cd65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Triticum
    123 rdf:type schema:DefinedTerm
    124 N4c48ca80660049a28fa5475ecd04770c rdf:first sg:person.01230730044.05
    125 rdf:rest N1d04960daea1425da81fe00659957681
    126 N57dddbf4cd20420c8f5bbf651cec3abe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Base Sequence
    128 rdf:type schema:DefinedTerm
    129 N5a531ee157fd483a928b10c79cf9641e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Gene Expression
    131 rdf:type schema:DefinedTerm
    132 N60e2c13480b04dab9c00bb7b6d0eee47 schema:name dimensions_id
    133 schema:value pub.1012528307
    134 rdf:type schema:PropertyValue
    135 N68620affb3fb4008ab974adb933a8c77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Gene Expression Profiling
    137 rdf:type schema:DefinedTerm
    138 N8506e7f6f87849b1aca00ee586930ab9 schema:name Springer Nature - SN SciGraph project
    139 rdf:type schema:Organization
    140 N881cbd39b5cb414c902b0b797b8bed5d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Recombinant Proteins
    142 rdf:type schema:DefinedTerm
    143 N960abd5083f84cb2976b370b5fbc78fd rdf:first sg:person.014011023502.91
    144 rdf:rest rdf:nil
    145 N987f7c7221524502a440390491eb7a12 schema:name Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    146 rdf:type schema:Organization
    147 N9cccfd00ef854e81a8dbb4aba925d062 rdf:first sg:person.01006262567.63
    148 rdf:rest Nda547ffdf39a4ae097fe3e64ddab25d3
    149 Na463b9d7d80f4b2aaf3554da34e69f33 schema:name nlm_unique_id
    150 schema:value 9209120
    151 rdf:type schema:PropertyValue
    152 Nb0fe4b9172e54e3fb8a788ab667e63ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Oryza
    154 rdf:type schema:DefinedTerm
    155 Nc098a9846ca54702bc4a8d31898718f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name In Vitro Techniques
    157 rdf:type schema:DefinedTerm
    158 Nc8d19c6a89fd43c584e7557c435dfd26 schema:affiliation Nfab5c28dc615482b9066f6c768c43930
    159 schema:familyName Goding
    160 schema:givenName Jeff
    161 rdf:type schema:Person
    162 Ncd0a415c9f6640018995a15a465309a1 schema:name Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    163 rdf:type schema:Organization
    164 Nd41152587011423e9c960f1766cf68b8 schema:issueNumber 5
    165 rdf:type schema:PublicationIssue
    166 Nd8945ee532fd4e7b8cef7cb1d842075f schema:name Arcadia Bioscience, 202 Cousteau Ct., 95616, Davis, CA, USA
    167 Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    168 rdf:type schema:Organization
    169 Nda547ffdf39a4ae097fe3e64ddab25d3 rdf:first sg:person.01300502704.66
    170 rdf:rest N960abd5083f84cb2976b370b5fbc78fd
    171 Ndb1f86f8353d42aea6d1feee8261d517 schema:affiliation Nfe3d99166a2b4241a83a91239d41ac78
    172 schema:familyName Hennegan
    173 schema:givenName Kevin
    174 rdf:type schema:Person
    175 Ne640779d6e6a4d81bc8d21734d767c60 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    176 schema:name Protein Sorting Signals
    177 rdf:type schema:DefinedTerm
    178 Ne889cead3487463cba6ef4f1d983d871 rdf:first Ndb1f86f8353d42aea6d1feee8261d517
    179 rdf:rest N07be7c7d52cd4d0494430b9d31d3b8ae
    180 Nf2af36d6cf904c0ab0aa4543389ad8e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    181 schema:name Glutens
    182 rdf:type schema:DefinedTerm
    183 Nf8e025180eec48ef8c9f8f607e672ea0 schema:name readcube_id
    184 schema:value 3bb0269183d0b65039fb9316edc766bd2b06b333b41a57c0f476e159f84526ed
    185 rdf:type schema:PropertyValue
    186 Nfab5c28dc615482b9066f6c768c43930 schema:name Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    187 rdf:type schema:Organization
    188 Nfe3d99166a2b4241a83a91239d41ac78 schema:name Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
    189 rdf:type schema:Organization
    190 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    191 schema:name Biological Sciences
    192 rdf:type schema:DefinedTerm
    193 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    194 schema:name Biochemistry and Cell Biology
    195 rdf:type schema:DefinedTerm
    196 sg:journal.1102526 schema:issn 0962-8819
    197 1573-9368
    198 schema:name Transgenic Research
    199 rdf:type schema:Periodical
    200 sg:person.01006262567.63 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
    201 schema:familyName Guo
    202 schema:givenName Fengli
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006262567.63
    204 rdf:type schema:Person
    205 sg:person.01047130531.26 schema:affiliation N1478b9de988d40f09de26b523fbe2012
    206 schema:familyName Nguyen
    207 schema:givenName Diane
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047130531.26
    209 rdf:type schema:Person
    210 sg:person.01136175674.75 schema:affiliation N1747ac7a340842e292efa5bb66f37ec6
    211 schema:familyName Yang
    212 schema:givenName Daichang
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136175674.75
    214 rdf:type schema:Person
    215 sg:person.01230730044.05 schema:affiliation Nd8945ee532fd4e7b8cef7cb1d842075f
    216 schema:familyName Wu
    217 schema:givenName Liying
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230730044.05
    219 rdf:type schema:Person
    220 sg:person.01300502704.66 schema:affiliation N987f7c7221524502a440390491eb7a12
    221 schema:familyName Huang
    222 schema:givenName Ning
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300502704.66
    224 rdf:type schema:Person
    225 sg:person.014011023502.91 schema:affiliation https://www.grid.ac/institutes/grid.21925.3d
    226 schema:familyName Watkins
    227 schema:givenName Simon C.
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014011023502.91
    229 rdf:type schema:Person
    230 sg:person.0757010643.55 schema:affiliation Ncd0a415c9f6640018995a15a465309a1
    231 schema:familyName Huang
    232 schema:givenName Jianmin
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757010643.55
    234 rdf:type schema:Person
    235 sg:pub.10.1007/bf02712670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003131267
    236 https://doi.org/10.1007/bf02712670
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1007/s001220100713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027805331
    239 https://doi.org/10.1007/s001220100713
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1007/s00299-001-0406-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1048483169
    242 https://doi.org/10.1007/s00299-001-0406-z
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1007/s00425-002-0919-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047760803
    245 https://doi.org/10.1007/s00425-002-0919-x
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1023/a:1006020208380 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050613832
    248 https://doi.org/10.1023/a:1006020208380
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1023/a:1006032627036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034710676
    251 https://doi.org/10.1023/a:1006032627036
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1023/a:1006194326804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040809660
    254 https://doi.org/10.1023/a:1006194326804
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1023/a:1006301519427 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019195519
    257 https://doi.org/10.1023/a:1006301519427
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1023/a:1008977630179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003922442
    260 https://doi.org/10.1023/a:1008977630179
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1023/a:1009648532210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018305891
    263 https://doi.org/10.1023/a:1009648532210
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1023/a:1009676322162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038951188
    266 https://doi.org/10.1023/a:1009676322162
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1023/a:1020355511981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028519364
    269 https://doi.org/10.1023/a:1020355511981
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/76523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050784753
    272 https://doi.org/10.1038/76523
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/84435 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000490996
    275 https://doi.org/10.1038/84435
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1016/0006-3002(52)90045-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016042812
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1016/0092-8674(93)90576-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1052790323
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1016/s0168-9452(01)00447-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043773491
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1016/s0168-9452(02)00165-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005188956
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1016/s0168-9452(98)00121-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035403433
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1016/s1360-1385(99)01389-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008859318
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1046/j.1365-313x.2003.01636.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037655632
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1046/j.1467-7652.2003.00038.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034221632
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1073/pnas.030527497 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033144170
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1073/pnas.1836901100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050644461
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1073/pnas.201411298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030999866
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1073/pnas.97.25.13512 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023326528
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1094/mpmi.2001.14.10.1255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060080314
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1105/tpc.7.7.945 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020002066
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.2135/cropsci2002.0278 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069027450
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.2135/cropsci2002.2780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090703518
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.2307/3870049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467989
    310 rdf:type schema:CreativeWork
    311 https://www.grid.ac/institutes/grid.21925.3d schema:alternateName University of Pittsburgh
    312 schema:name Department of Cell Biology and Physiology, University of Pittsburgh, 15261, PA, USA
    313 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...