The effect of additional virulence genes on transformation efficiency, transgene integration and expression in rice plants using the pGreen/pSoup dual ... View Full Text


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Article Info

DATE

2004-12

AUTHORS

Philippe Vain, Alison Harvey, Barbara Worland, Shona Ross, John W. Snape, David Lonsdale

ABSTRACT

We assessed the effect of four different virulence (vir) gene combinations on plant transformation efficiency and transgene behaviour in rice using the pGreen/pSoup dual binary vector system. Transformation experiments were conducted using a pGreen vector containing the bar and gusA expression units with, or without, the virG542, virGN54D, virGwt or the virG/B/C genes added to the backbone. Additonal vir gene(s) significantly altered plant transformation efficiency and the integration of vector backbone sequences. However, no differences in transgene copy number, percentage of expressing lines and expression levels could be detected. Addition of virGwt was the most beneficial, doubling the overall performance of the pGreen/pSoup vector system based on transformation frequency, absence of backbone sequence integration and expression of unselected transgenes. In 39 of the plant lines, the additional vir genes were integrated into the rice genome. The contribution of ‘super dual binary’ pGreen/pSoup vectors to the development of efficient rice transformation systems and to the production of plants free of selectable marker genes are discussed. More... »

PAGES

593-603

References to SciGraph publications

  • 2002-09. Transposon-mediated generation of T-DNA- and marker-free rice plants expressing a Bt endotoxin gene in MOLECULAR BREEDING
  • 2000-04. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation in PLANT MOLECULAR BIOLOGY
  • 2004-05-15. A large-scale study of rice plants transformed with different T-DNAs provides new insights into locus composition and T-DNA linkage configurations in THEORETICAL AND APPLIED GENETICS
  • 2001-02. High-efficiency gene transfer to recalcitrant plants by Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 2002-11-26. Additional virulence genes and sonication enhance Agrobacterium tumefaciens-mediated loblolly pine transformation in PLANT CELL REPORTS
  • 2000-02. Evidence of multiple complex patterns of T-DNA integration into the rice genome in THEORETICAL AND APPLIED GENETICS
  • 2000-11. Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation in THEORETICAL AND APPLIED GENETICS
  • 1999-02. High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda) in PLANT MOLECULAR BIOLOGY
  • 1996-06. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens in NATURE BIOTECHNOLOGY
  • 2003-04-03. Transgene behaviour in populations of rice plants transformed using a new dual binary vector system: pGreen/pSoup in THEORETICAL AND APPLIED GENETICS
  • 1992-12. Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens in PLANT MOLECULAR BIOLOGY
  • 2001-09. Characterization of rice transformed via an Agrobacterium-mediated inflorescence approach in MOLECULAR BREEDING
  • 2003-04-03. Highly efficient production and characterization of T-DNA plants for rice (Oryza sativa L.) functional genomics in THEORETICAL AND APPLIED GENETICS
  • 1990-10. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542 in PLANT CELL REPORTS
  • 2002-08-22. Transgene behaviour across two generations in a large random population of transgenic rice plants produced by particle bombardment in THEORETICAL AND APPLIED GENETICS
  • 2000-10. T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation in MOLECULAR BREEDING
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    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-004-2808-5

    DOI

    http://dx.doi.org/10.1007/s11248-004-2808-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004588142

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15672840


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