Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors View Full Text


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Article Info

DATE

2004-10

AUTHORS

Shihshieh Huang, Larry Gilbertson, Tom Adams, Kathleen Malloy, Emily Reisenbigler, Darren Birr, Mark Snyder, Qiang Zhang, Michael Luethy

ABSTRACT

By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant genome. This allows the recovery of marker-free transgenic plants through genetic segregation in the next generation. In this study, we have found that by repositioning the selectable marker gene in the backbone and leaving only the GOI in the T-DNA region, a regular two-border binary plasmid was able to generate marker-free transgenic maize plants more efficiently than a conventional single binary plasmid with multiple T-DNA borders. These results also provide evidence that both the right and left borders can initiate and terminate T-strands. Such non-canonical initiation and termination of T-strands may be the basis for the elevated frequencies of cotransformation and unlinked insertions. More... »

PAGES

451-461

References to SciGraph publications

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  • 2003-07. Transgene behaviour in populations of rice plants transformed using a new dual binary vector system: pGreen/pSoup in THEORETICAL AND APPLIED GENETICS
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  • 1987-12. Sequence context of the T-DNA border repeat element determines its relative activity during T-DNA transfer to plant cells in MOLECULAR GENETICS AND GENOMICS
  • 1991-09. Two T-DNA's co-transformed intoBrassica napus by a doubleAgrobacterium tumefaciens infection are mainly integrated at the same locus in THEORETICAL AND APPLIED GENETICS
  • 1983-11. Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955 in PLANT MOLECULAR BIOLOGY
  • 2002-06. Excision of selectable marker genes from transgenic plants in NATURE BIOTECHNOLOGY
  • 2001-04. Efficient Co-Transformation of Nicotiana Tabacum by two Independent T-DNAs, the Effect of T-DNA Size and Implications for Genetic Separation in TRANSGENIC RESEARCH
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11248-004-1453-3

    DOI

    http://dx.doi.org/10.1007/s11248-004-1453-3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006664602

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15587269


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