Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-10

AUTHORS

Shihshieh Huang, Larry Gilbertson, Tom Adams, Kathleen Malloy, Emily Reisenbigler, Darren Birr, Mark Snyder, Qiang Zhang, Michael Luethy

ABSTRACT

By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant genome. This allows the recovery of marker-free transgenic plants through genetic segregation in the next generation. In this study, we have found that by repositioning the selectable marker gene in the backbone and leaving only the GOI in the T-DNA region, a regular two-border binary plasmid was able to generate marker-free transgenic maize plants more efficiently than a conventional single binary plasmid with multiple T-DNA borders. These results also provide evidence that both the right and left borders can initiate and terminate T-strands. Such non-canonical initiation and termination of T-strands may be the basis for the elevated frequencies of cotransformation and unlinked insertions. More... »

PAGES

451-461

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11248-004-1453-3

DOI

http://dx.doi.org/10.1007/s11248-004-1453-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006664602

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15587269


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