Unveiling the future reservoir of anti-cancer molecule—Combretastatin A4 from callus and cell aggregate suspension culture of flame creeper (Combretum microphyllum): ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-10-21

AUTHORS

Harshita Pandey, Pallavi Pandey, Sailendra Singh, Arvind Singh Negi, Suchitra Banerjee

ABSTRACT

Combretastatin A4 (CA4), a natural stilbenoid molecule, has currently gained noticeable prominence for holding outstanding anti-angiogenic and selective vascular-targeting competency combined with renowned potential to prevent tumour metastasis. Efficacious preclinical insights against several types of cancer revealed better druggability potentials of specialized derivative(s) of this molecule. Considering such therapeutic significance and scanty natural availability, the competence of callus and compact cell aggregates (CCA)—suspension cultures of Combretum microphyllum had been explored revealing their untouched potentials as effective production alternatives for CA4. The CCA-suspension culture accumulated the highest amount of the CA4 (2.29 ± 0.04 mg/g DW) at 12 weeks cultivation period, which was 2.26 fold higher over that in 3 years old in-vivo plant leaves (1.01 ± 0.05 mg/g DW). In comparison, the calli (grown with TDZ) showed 1.89 fold lesser yield of CA4 (1.21 ± 0.05 mg/g DW) than the CCA-suspension culture. Exudation of CA4 in CCA-suspension cultures was evident throughout culture duration with the highest level (70.03%) at the maximum production phase. Compared to untreated control CCA-suspension culture, methyl-jasmonate (MeJ) elicitation first time ascertained 2.26 fold improvement of CA4 yield with noticeable stimulation of exudation. Observably, the longer duration (48hrs) and the higher dose (200 µm) of MeJ treatment proved optimum for the maximum enhancement of CA4 yield in CCA-suspension cultures. Present findings unveiled an unprecedented avenue towards fulfilling the rising commercial requirement of CA4 for future anti-cancer drug development processes through strategic implementation of in-vitro cultures options in C. microphyllum including MeJ elicitation and exudation. More... »

PAGES

681-691

References to SciGraph publications

  • 2017-11-09. Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2001-01. Compact callus cluster suspension cultures of Catharanthus roseus with enhanced indole alkaloid biosynthesis in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1996-02. Taxol production in suspension cultures of Taxus baccata in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2017-06-09. Micropropagation of Combretum ovalifolium Roxb.: a medicinally important plant in RENDICONTI LINCEI. SCIENZE FISICHE E NATURALI
  • 1998-10. Thidiazuron: A potent regulator ofin vitro plant morphogenesis in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2012-10-09. Cellular aggregation is a key parameter associated with long term variability in paclitaxel accumulation in Taxus suspension cultures in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2011-10-11. Exudation: an expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures in PLANT CELL REPORTS
  • 2016-06-18. Meeting the challenge of stevioside production in the hairy roots of Stevia rebaudiana by probing the underlying process in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2019-01-17. Ocimum basilicum suspension culture as resource for bioactive triterpenoids: yield enrichment by elicitation and bioreactor cultivation in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2013-12-28. Thidiazuron-Induced Changes in Biomass Parameters, Total Phenolic Content, and Antioxidant Activity in Callus Cultures of Artemisia absinthium L. in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2018-03-24. Thidiazuron as an Elicitor in the Production of Secondary Metabolite in THIDIAZURON: FROM UREA DERIVATIVE TO PLANT GROWTH REGULATOR
  • 2001-06. Effects of stress factors, bioregulators, and synthetic precursors on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2012-04-06. In vitro propagation and secondary product production by Merwilla plumbea (Lindl.) Speta in PLANT GROWTH REGULATION
  • 2018-10-06. Influence of thidiazuron on callus induction and crocin production in corm and style explants of Crocus sativus L. in ACTA PHYSIOLOGIAE PLANTARUM
  • 2011-02-04. Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2017-09-06. Isolation and characterization of the compounds responsible for the antimutagenic activity of Combretum microphyllum (Combretaceae) leaf extracts in BMC COMPLEMENTARY MEDICINE AND THERAPIES
  • 2018-03-24. TDZ Induced Diverse In Vitro Responses in Some Economically Important Plants in THIDIAZURON: FROM UREA DERIVATIVE TO PLANT GROWTH REGULATOR
  • 2003-05. Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2014-03-16. Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2018-07-24. Exploring Medicinal Plant Legacy for Drug Discovery in Post-genomic Era in PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, INDIA SECTION B: BIOLOGICAL SCIENCES
  • 2016-01-13. Differential Effects of Thidiazuron on Production of Anticancer Phenolic Compounds in Callus Cultures of Fagonia indica in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1989-02. Isolation and structure of the strong cell growth and tubulin inhibitor combretastatin A-4 in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2015-04-11. The influence of TDZ concentrations on in vitro growth and production of secondary metabolites by the shoot and callus culture of Lallemantia iberica in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2014-10-12. Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation in PROTOPLASMA
  • 2003-06-17. A simple method for enhancing paclitaxel release from Taxus canadensis cell suspension cultures utilizing cell wall digesting enzymes in PLANT CELL REPORTS
  • 2016-04-05. Chemical elicitors versus secondary metabolite production in vitro using plant cell, tissue and organ cultures: recent trends and a sky eye view appraisal in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2017. Recent Advancements in the Clinical Evaluation of Plant-Derived Anticancer Compounds in ANTICANCER PLANTS: CLINICAL TRIALS AND NANOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11240-020-01951-x

    DOI

    http://dx.doi.org/10.1007/s11240-020-01951-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1131954085


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Agricultural and Veterinary Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Crop and Pasture Production", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pandey", 
            "givenName": "Harshita", 
            "id": "sg:person.0673014242.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673014242.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pandey", 
            "givenName": "Pallavi", 
            "id": "sg:person.01026741264.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026741264.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Singh", 
            "givenName": "Sailendra", 
            "id": "sg:person.0677772762.58", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677772762.58"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medicinal Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Medicinal Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Negi", 
            "givenName": "Arvind Singh", 
            "id": "sg:person.01163477424.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163477424.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India", 
              "id": "http://www.grid.ac/institutes/grid.417631.6", 
              "name": [
                "Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Banerjee", 
            "givenName": "Suchitra", 
            "id": "sg:person.01243703443.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243703443.05"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf02822732", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031895499", 
              "https://doi.org/10.1007/bf02822732"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12906-017-1935-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091486786", 
              "https://doi.org/10.1186/s12906-017-1935-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12010-016-1978-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053414320", 
              "https://doi.org/10.1007/s12010-016-1978-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-015-0769-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022015601", 
              "https://doi.org/10.1007/s11240-015-0769-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1022806420209", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031276377", 
              "https://doi.org/10.1023/a:1022806420209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10725-012-9682-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031469790", 
              "https://doi.org/10.1007/s10725-012-9682-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01954881", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019036279", 
              "https://doi.org/10.1007/bf01954881"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-012-0237-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035336242", 
              "https://doi.org/10.1007/s11240-012-0237-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11627-001-0013-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022037016", 
              "https://doi.org/10.1007/s11627-001-0013-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00048185", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010201977", 
              "https://doi.org/10.1007/bf00048185"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-017-1332-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092589510", 
              "https://doi.org/10.1007/s11240-017-1332-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002530000568", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037880546", 
              "https://doi.org/10.1007/s002530000568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00709-014-0711-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045750196", 
              "https://doi.org/10.1007/s00709-014-0711-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-981-10-8216-0_8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105290254", 
              "https://doi.org/10.1007/978-981-10-8216-0_8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-018-01552-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1111499337", 
              "https://doi.org/10.1007/s11240-018-01552-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-003-0575-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024966098", 
              "https://doi.org/10.1007/s00299-003-0575-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s40011-018-1013-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105804859", 
              "https://doi.org/10.1007/s40011-018-1013-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-011-9919-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014974061", 
              "https://doi.org/10.1007/s11240-011-9919-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12210-017-0625-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085933534", 
              "https://doi.org/10.1007/s12210-017-0625-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-981-10-8004-3_17", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101719379", 
              "https://doi.org/10.1007/978-981-10-8004-3_17"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-014-0467-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037338629", 
              "https://doi.org/10.1007/s11240-014-0467-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12010-013-0663-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038933392", 
              "https://doi.org/10.1007/s12010-013-0663-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-016-1020-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019473095", 
              "https://doi.org/10.1007/s11240-016-1020-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-981-10-8004-3_27", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101719390", 
              "https://doi.org/10.1007/978-981-10-8004-3_27"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11738-018-2760-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1107436803", 
              "https://doi.org/10.1007/s11738-018-2760-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00299-011-1165-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029444030", 
              "https://doi.org/10.1007/s00299-011-1165-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11240-016-0985-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011961349", 
              "https://doi.org/10.1007/s11240-016-0985-6"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2020-10-21", 
        "datePublishedReg": "2020-10-21", 
        "description": "Combretastatin A4 (CA4), a natural stilbenoid molecule, has currently gained noticeable prominence for holding outstanding anti-angiogenic and selective vascular-targeting competency combined with renowned potential to prevent tumour metastasis. Efficacious preclinical insights against several types of cancer revealed better druggability potentials of specialized derivative(s) of this molecule. Considering such therapeutic significance and scanty natural availability, the competence of callus and compact cell aggregates (CCA)\u2014suspension cultures of Combretum microphyllum had been explored revealing their untouched potentials as effective production alternatives for CA4. The CCA-suspension culture accumulated the highest amount of the CA4 (2.29\u2009\u00b1\u20090.04\u00a0mg/g DW) at 12\u00a0weeks cultivation period, which was 2.26 fold higher over that in 3\u00a0years old in-vivo plant leaves (1.01\u2009\u00b1\u20090.05\u00a0mg/g DW). In comparison, the calli (grown with TDZ) showed 1.89 fold lesser yield of CA4 (1.21\u2009\u00b1\u20090.05\u00a0mg/g DW) than the CCA-suspension culture. Exudation of CA4 in CCA-suspension cultures was evident throughout culture duration with the highest level (70.03%) at the maximum production phase. Compared to untreated control CCA-suspension culture, methyl-jasmonate (MeJ) elicitation first time ascertained 2.26 fold improvement of CA4 yield with noticeable stimulation of exudation. Observably, the longer duration (48hrs) and the higher dose (200\u00a0\u00b5m) of MeJ treatment proved optimum for the maximum enhancement of CA4 yield in CCA-suspension cultures. Present findings unveiled an unprecedented avenue towards fulfilling the rising commercial requirement of CA4 for future anti-cancer drug development processes through strategic implementation of in-vitro cultures options in C. microphyllum including MeJ elicitation and exudation.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s11240-020-01951-x", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1123614", 
            "issn": [
              "0167-6857", 
              "1573-5044"
            ], 
            "name": "Plant Cell, Tissue and Organ Culture (PCTOC)", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "143"
          }
        ], 
        "keywords": [
          "combretastatin A4", 
          "types of cancer", 
          "high dose", 
          "preclinical insights", 
          "therapeutic significance", 
          "tumor metastasis", 
          "drug development process", 
          "longer duration", 
          "present findings", 
          "druggability potential", 
          "noticeable prominence", 
          "duration", 
          "exudation", 
          "high levels", 
          "metastasis", 
          "cancer", 
          "dose", 
          "A4", 
          "stimulation", 
          "culture duration", 
          "treatment", 
          "cell aggregates", 
          "culture", 
          "noticeable stimulation", 
          "compact cell aggregates", 
          "weeks cultivation period", 
          "years", 
          "options", 
          "high amounts", 
          "findings", 
          "potential", 
          "period", 
          "levels", 
          "significance", 
          "study", 
          "improvement", 
          "first time", 
          "molecules", 
          "elicitation study", 
          "suspension cultures", 
          "types", 
          "callus", 
          "alternative", 
          "time", 
          "MeJ treatment", 
          "elicitation", 
          "competencies", 
          "comparison", 
          "avenues", 
          "availability", 
          "competence", 
          "growth", 
          "prominence", 
          "insights", 
          "amount", 
          "fold improvement", 
          "maximum enhancement", 
          "enhancement", 
          "strategic implementation", 
          "unprecedented avenues", 
          "phase", 
          "future reservoir", 
          "implementation", 
          "reservoir", 
          "aggregates", 
          "microphyllum", 
          "cultivation period", 
          "requirements", 
          "development process", 
          "process", 
          "natural availability", 
          "leaves", 
          "creeper", 
          "plant leaves", 
          "less yield", 
          "yield", 
          "C. microphyllum", 
          "production phase", 
          "production alternatives", 
          "commercial requirements"
        ], 
        "name": "Unveiling the future reservoir of anti-cancer molecule\u2014Combretastatin A4 from callus and cell aggregate suspension culture of flame creeper (Combretum microphyllum): growth, exudation and elicitation studies", 
        "pagination": "681-691", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1131954085"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11240-020-01951-x"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11240-020-01951-x", 
          "https://app.dimensions.ai/details/publication/pub.1131954085"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-05-20T07:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_831.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s11240-020-01951-x"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11240-020-01951-x'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11240-020-01951-x'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11240-020-01951-x'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11240-020-01951-x'


     

    This table displays all metadata directly associated to this object as RDF triples.

    284 TRIPLES      22 PREDICATES      134 URIs      97 LITERALS      6 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11240-020-01951-x schema:about anzsrc-for:06
    2 anzsrc-for:0607
    3 anzsrc-for:07
    4 anzsrc-for:0703
    5 schema:author Na5b8e90861944416bd1dd3ee762e9391
    6 schema:citation sg:pub.10.1007/978-981-10-8004-3_17
    7 sg:pub.10.1007/978-981-10-8004-3_27
    8 sg:pub.10.1007/978-981-10-8216-0_8
    9 sg:pub.10.1007/bf00048185
    10 sg:pub.10.1007/bf01954881
    11 sg:pub.10.1007/bf02822732
    12 sg:pub.10.1007/s002530000568
    13 sg:pub.10.1007/s00299-003-0575-z
    14 sg:pub.10.1007/s00299-011-1165-0
    15 sg:pub.10.1007/s00709-014-0711-3
    16 sg:pub.10.1007/s10725-012-9682-6
    17 sg:pub.10.1007/s11240-011-9919-5
    18 sg:pub.10.1007/s11240-012-0237-3
    19 sg:pub.10.1007/s11240-014-0467-7
    20 sg:pub.10.1007/s11240-015-0769-4
    21 sg:pub.10.1007/s11240-016-0985-6
    22 sg:pub.10.1007/s11240-016-1020-7
    23 sg:pub.10.1007/s11240-017-1332-2
    24 sg:pub.10.1007/s11240-018-01552-9
    25 sg:pub.10.1007/s11627-001-0013-2
    26 sg:pub.10.1007/s11738-018-2760-2
    27 sg:pub.10.1007/s12010-013-0663-7
    28 sg:pub.10.1007/s12010-016-1978-y
    29 sg:pub.10.1007/s12210-017-0625-z
    30 sg:pub.10.1007/s40011-018-1013-x
    31 sg:pub.10.1023/a:1022806420209
    32 sg:pub.10.1186/s12906-017-1935-5
    33 schema:datePublished 2020-10-21
    34 schema:datePublishedReg 2020-10-21
    35 schema:description Combretastatin A4 (CA4), a natural stilbenoid molecule, has currently gained noticeable prominence for holding outstanding anti-angiogenic and selective vascular-targeting competency combined with renowned potential to prevent tumour metastasis. Efficacious preclinical insights against several types of cancer revealed better druggability potentials of specialized derivative(s) of this molecule. Considering such therapeutic significance and scanty natural availability, the competence of callus and compact cell aggregates (CCA)—suspension cultures of Combretum microphyllum had been explored revealing their untouched potentials as effective production alternatives for CA4. The CCA-suspension culture accumulated the highest amount of the CA4 (2.29 ± 0.04 mg/g DW) at 12 weeks cultivation period, which was 2.26 fold higher over that in 3 years old in-vivo plant leaves (1.01 ± 0.05 mg/g DW). In comparison, the calli (grown with TDZ) showed 1.89 fold lesser yield of CA4 (1.21 ± 0.05 mg/g DW) than the CCA-suspension culture. Exudation of CA4 in CCA-suspension cultures was evident throughout culture duration with the highest level (70.03%) at the maximum production phase. Compared to untreated control CCA-suspension culture, methyl-jasmonate (MeJ) elicitation first time ascertained 2.26 fold improvement of CA4 yield with noticeable stimulation of exudation. Observably, the longer duration (48hrs) and the higher dose (200 µm) of MeJ treatment proved optimum for the maximum enhancement of CA4 yield in CCA-suspension cultures. Present findings unveiled an unprecedented avenue towards fulfilling the rising commercial requirement of CA4 for future anti-cancer drug development processes through strategic implementation of in-vitro cultures options in C. microphyllum including MeJ elicitation and exudation.
    36 schema:genre article
    37 schema:inLanguage en
    38 schema:isAccessibleForFree false
    39 schema:isPartOf N1dd3475752d546c9af56b4edc630861d
    40 N5c0e0bfc493b4fecb6dbaf9632f13e19
    41 sg:journal.1123614
    42 schema:keywords A4
    43 C. microphyllum
    44 MeJ treatment
    45 aggregates
    46 alternative
    47 amount
    48 availability
    49 avenues
    50 callus
    51 cancer
    52 cell aggregates
    53 combretastatin A4
    54 commercial requirements
    55 compact cell aggregates
    56 comparison
    57 competence
    58 competencies
    59 creeper
    60 cultivation period
    61 culture
    62 culture duration
    63 development process
    64 dose
    65 drug development process
    66 druggability potential
    67 duration
    68 elicitation
    69 elicitation study
    70 enhancement
    71 exudation
    72 findings
    73 first time
    74 fold improvement
    75 future reservoir
    76 growth
    77 high amounts
    78 high dose
    79 high levels
    80 implementation
    81 improvement
    82 insights
    83 leaves
    84 less yield
    85 levels
    86 longer duration
    87 maximum enhancement
    88 metastasis
    89 microphyllum
    90 molecules
    91 natural availability
    92 noticeable prominence
    93 noticeable stimulation
    94 options
    95 period
    96 phase
    97 plant leaves
    98 potential
    99 preclinical insights
    100 present findings
    101 process
    102 production alternatives
    103 production phase
    104 prominence
    105 requirements
    106 reservoir
    107 significance
    108 stimulation
    109 strategic implementation
    110 study
    111 suspension cultures
    112 therapeutic significance
    113 time
    114 treatment
    115 tumor metastasis
    116 types
    117 types of cancer
    118 unprecedented avenues
    119 weeks cultivation period
    120 years
    121 yield
    122 schema:name Unveiling the future reservoir of anti-cancer molecule—Combretastatin A4 from callus and cell aggregate suspension culture of flame creeper (Combretum microphyllum): growth, exudation and elicitation studies
    123 schema:pagination 681-691
    124 schema:productId N4d61cbc020f14f7db6b06d16ef134052
    125 Nb642875f05054923ab534bd138207425
    126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1131954085
    127 https://doi.org/10.1007/s11240-020-01951-x
    128 schema:sdDatePublished 2022-05-20T07:36
    129 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    130 schema:sdPublisher N9f51f0f7ed18436c942248f2d5efc361
    131 schema:url https://doi.org/10.1007/s11240-020-01951-x
    132 sgo:license sg:explorer/license/
    133 sgo:sdDataset articles
    134 rdf:type schema:ScholarlyArticle
    135 N1dd3475752d546c9af56b4edc630861d schema:volumeNumber 143
    136 rdf:type schema:PublicationVolume
    137 N2c6412b2114d45a88b84d438843e9d06 rdf:first sg:person.01163477424.63
    138 rdf:rest Nb6d797b2cc59433da37b148c6638df38
    139 N2fd124f2b582496ca25904393c801cf6 rdf:first sg:person.01026741264.28
    140 rdf:rest Nc9db7b97f9b64956a808b4443b708497
    141 N4d61cbc020f14f7db6b06d16ef134052 schema:name dimensions_id
    142 schema:value pub.1131954085
    143 rdf:type schema:PropertyValue
    144 N5c0e0bfc493b4fecb6dbaf9632f13e19 schema:issueNumber 3
    145 rdf:type schema:PublicationIssue
    146 N9f51f0f7ed18436c942248f2d5efc361 schema:name Springer Nature - SN SciGraph project
    147 rdf:type schema:Organization
    148 Na5b8e90861944416bd1dd3ee762e9391 rdf:first sg:person.0673014242.37
    149 rdf:rest N2fd124f2b582496ca25904393c801cf6
    150 Nb642875f05054923ab534bd138207425 schema:name doi
    151 schema:value 10.1007/s11240-020-01951-x
    152 rdf:type schema:PropertyValue
    153 Nb6d797b2cc59433da37b148c6638df38 rdf:first sg:person.01243703443.05
    154 rdf:rest rdf:nil
    155 Nc9db7b97f9b64956a808b4443b708497 rdf:first sg:person.0677772762.58
    156 rdf:rest N2c6412b2114d45a88b84d438843e9d06
    157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Biological Sciences
    159 rdf:type schema:DefinedTerm
    160 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    161 schema:name Plant Biology
    162 rdf:type schema:DefinedTerm
    163 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
    164 schema:name Agricultural and Veterinary Sciences
    165 rdf:type schema:DefinedTerm
    166 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
    167 schema:name Crop and Pasture Production
    168 rdf:type schema:DefinedTerm
    169 sg:journal.1123614 schema:issn 0167-6857
    170 1573-5044
    171 schema:name Plant Cell, Tissue and Organ Culture (PCTOC)
    172 schema:publisher Springer Nature
    173 rdf:type schema:Periodical
    174 sg:person.01026741264.28 schema:affiliation grid-institutes:grid.417631.6
    175 schema:familyName Pandey
    176 schema:givenName Pallavi
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026741264.28
    178 rdf:type schema:Person
    179 sg:person.01163477424.63 schema:affiliation grid-institutes:grid.417631.6
    180 schema:familyName Negi
    181 schema:givenName Arvind Singh
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163477424.63
    183 rdf:type schema:Person
    184 sg:person.01243703443.05 schema:affiliation grid-institutes:grid.417631.6
    185 schema:familyName Banerjee
    186 schema:givenName Suchitra
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243703443.05
    188 rdf:type schema:Person
    189 sg:person.0673014242.37 schema:affiliation grid-institutes:grid.417631.6
    190 schema:familyName Pandey
    191 schema:givenName Harshita
    192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673014242.37
    193 rdf:type schema:Person
    194 sg:person.0677772762.58 schema:affiliation grid-institutes:grid.417631.6
    195 schema:familyName Singh
    196 schema:givenName Sailendra
    197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677772762.58
    198 rdf:type schema:Person
    199 sg:pub.10.1007/978-981-10-8004-3_17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101719379
    200 https://doi.org/10.1007/978-981-10-8004-3_17
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1007/978-981-10-8004-3_27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101719390
    203 https://doi.org/10.1007/978-981-10-8004-3_27
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1007/978-981-10-8216-0_8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105290254
    206 https://doi.org/10.1007/978-981-10-8216-0_8
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1007/bf00048185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010201977
    209 https://doi.org/10.1007/bf00048185
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1007/bf01954881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019036279
    212 https://doi.org/10.1007/bf01954881
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1007/bf02822732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031895499
    215 https://doi.org/10.1007/bf02822732
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1007/s002530000568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037880546
    218 https://doi.org/10.1007/s002530000568
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1007/s00299-003-0575-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1024966098
    221 https://doi.org/10.1007/s00299-003-0575-z
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1007/s00299-011-1165-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029444030
    224 https://doi.org/10.1007/s00299-011-1165-0
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1007/s00709-014-0711-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045750196
    227 https://doi.org/10.1007/s00709-014-0711-3
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1007/s10725-012-9682-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031469790
    230 https://doi.org/10.1007/s10725-012-9682-6
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1007/s11240-011-9919-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014974061
    233 https://doi.org/10.1007/s11240-011-9919-5
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1007/s11240-012-0237-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035336242
    236 https://doi.org/10.1007/s11240-012-0237-3
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1007/s11240-014-0467-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037338629
    239 https://doi.org/10.1007/s11240-014-0467-7
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1007/s11240-015-0769-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022015601
    242 https://doi.org/10.1007/s11240-015-0769-4
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1007/s11240-016-0985-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011961349
    245 https://doi.org/10.1007/s11240-016-0985-6
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1007/s11240-016-1020-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019473095
    248 https://doi.org/10.1007/s11240-016-1020-7
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1007/s11240-017-1332-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092589510
    251 https://doi.org/10.1007/s11240-017-1332-2
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1007/s11240-018-01552-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111499337
    254 https://doi.org/10.1007/s11240-018-01552-9
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1007/s11627-001-0013-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022037016
    257 https://doi.org/10.1007/s11627-001-0013-2
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1007/s11738-018-2760-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107436803
    260 https://doi.org/10.1007/s11738-018-2760-2
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1007/s12010-013-0663-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038933392
    263 https://doi.org/10.1007/s12010-013-0663-7
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1007/s12010-016-1978-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1053414320
    266 https://doi.org/10.1007/s12010-016-1978-y
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1007/s12210-017-0625-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1085933534
    269 https://doi.org/10.1007/s12210-017-0625-z
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1007/s40011-018-1013-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1105804859
    272 https://doi.org/10.1007/s40011-018-1013-x
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1023/a:1022806420209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031276377
    275 https://doi.org/10.1023/a:1022806420209
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1186/s12906-017-1935-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091486786
    278 https://doi.org/10.1186/s12906-017-1935-5
    279 rdf:type schema:CreativeWork
    280 grid-institutes:grid.417631.6 schema:alternateName Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India
    281 Medicinal Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India
    282 schema:name Department of Plant Biotechnology, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India
    283 Medicinal Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), 226015, Lucknow, Uttar Pradesh, India
    284 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...