Protocol for bonediol production in Bonellia macrocarpa hairy root culture View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-03-12

AUTHORS

Luis Alberto Ruiz-Ramírez, Gregorio del Carmen Godoy-Hernández, Peggy Elizabeth Álvarez-Gutiérrez, Víctor Manuel Ruíz-Valdiviezo, María Celina Lujan-Hidalgo, Elidé Avilés-Berzunza, Federico Antonio Gutiérrez-Miceli

ABSTRACT

The aim of this protocol was for Bonellia macrocarpa hairy root establishment and bonediol production in transformed root cultures. Cotyledons, hypocotyls and roots were used as explants for hairy root induction. Fluorescence microscopy was used for detected tandem dimer of the tomato protein (TDT). A PCR analysis for rolC was used to confirm the presence of these genes. Identification and quantification of bonediol was made for HPLC. Hypocotyls showed greater transformation efficiency (25%). Fluorescence microscopy and PCR analysis confirm genetic transformation. Bonedio production was higher in hairy roots than in complete plants. More... »

PAGES

177-181

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11240-018-1403-z

DOI

http://dx.doi.org/10.1007/s11240-018-1403-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1101511287


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