Meeting the challenge of stevioside production in the hairy roots of Stevia rebaudiana by probing the underlying process View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-06-18

AUTHORS

Harshita Pandey, Pallavi Pandey, Shiv Shanker Pandey, Sailendra Singh, Suchitra Banerjee

ABSTRACT

Agrobacterium rhizogenes mediated “hairy root cultures” of the renowned bio-sweetener producing plant—Stevia rebaudiana, were generated to explore their yet undetected biosynthetic potentials concerning the low calorie diterpene glycoside—stevioside. Four stable rhizoclones were studied under light and dark conditions showing better growth under dark. Two of these rhizoclones revealed substantially higher photosynthetic pigment accumulation under light. Evidently, capitalization on the available inter-clonal variability first time showed the stevioside synthesizing exclusivity in the SRA4 rhizoclone under light condition, while the rest failed. The dualities of the glycoside synthesizing power amongst the two photosynthetically active rhizoclones were resolved through quantitative RT-PCR analysis of UGT85C2 gene showing positive expression in the stevioside producing rhizoclone. These findings elucidated the decisive role of UGT85C2 in combination with photosynthetic proficiency of the studied hairy root cultures in regulating the biosynthetic pathway of S. rebaudiana. More... »

PAGES

511-521

References to SciGraph publications

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  • 2014-01-23. Effect of Salts (NaCl and Na2CO3) on Callus and Suspension Culture of Stevia rebaudiana for Steviol glycoside Production in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2015-08-28. Advances in Boerhaavia diffusa hairy root technology: a valuable pursuit for identifying strain sensitivity and up-scaling factors to refine metabolite yield and bioactivity potentials in PROTOPLASMA
  • 2011-10-11. Exudation: an expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures in PLANT CELL REPORTS
  • 2015-11-23. Enhanced trans-resveratrol production in genetically transformed root cultures of Peanut (Arachis hypogaea L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2014-06-04. Stevia rebaudiana Bertoni: An alternative Sugar Replacer and Its Application in Food Industry in FOOD ENGINEERING REVIEWS
  • 2011-02-13. Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2008-09-11. Hairy root culture of Plumbago indica as a potential source for plumbagin in BIOLOGIA PLANTARUM
  • 2014-10-12. Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation in PROTOPLASMA
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    http://scigraph.springernature.com/pub.10.1007/s11240-016-1020-7

    DOI

    http://dx.doi.org/10.1007/s11240-016-1020-7

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    47 combination
    48 conditions
    49 culture
    50 dark
    51 dark conditions
    52 decisive role
    53 diterpenes
    54 duality
    55 exclusivity
    56 expression
    57 findings
    58 first time
    59 genes
    60 growth
    61 hairy root cultures
    62 hairy roots
    63 light
    64 light conditions
    65 pathway
    66 photosynthetic pigment accumulation
    67 pigment accumulation
    68 plant Stevia rebaudiana
    69 positive expression
    70 potential
    71 power
    72 process
    73 production
    74 proficiency
    75 quantitative RT-PCR analysis
    76 rebaudiana
    77 rest
    78 rhizoclones
    79 rhizogenes
    80 role
    81 root cultures
    82 roots
    83 stevioside production
    84 time
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