Anther culture induces transposable element movement in potato View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-08-12

AUTHORS

Nan Lu, Bingyu Zhao, Andy Pereira, Vladimir Shulaev, Richard E. Veilleux

ABSTRACT

Transposon tagging is a powerful technology for studying gene function; however, somatic transposition prior to gametogenesis may result in multiple derivatives bearing the same insertion. In order to attempt to increase germinal transposition, we synthesized two novel transposon-tagging constructs. We placed the transposase gene under control of either of two microspore-specific promoters identified in Arabidopsis or Antirrhinum within the Ds element that also harbored kanamycin resistance. Both transposase and NPTII gene sequences were optimized in silico based on codon usage bias data in dicot species. Agrobacterium-mediated transformation was used to insert the constructs into diploid potato. The transgenic plants were subjected to both anther culture and outcrossing to wild type diploid potato to observe the opportunity for transposition in either gametophytic process. Green fluorescent protein screening was not efficient but kanamycin selection was able to eliminate 94 % of wild type progenies. Transposants were recovered following anther culture of two transgenic lines whereas no transposants were found among more than 250 T1 seedlings. Anther culture provided a unique venue for the observation of the influence of gametophye specific promoters on transposition in potato. More... »

PAGES

361-366

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11240-014-0576-3

DOI

http://dx.doi.org/10.1007/s11240-014-0576-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032319827


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA", 
          "id": "http://www.grid.ac/institutes/grid.438526.e", 
          "name": [
            "Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lu", 
        "givenName": "Nan", 
        "id": "sg:person.01011304627.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011304627.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA", 
          "id": "http://www.grid.ac/institutes/grid.438526.e", 
          "name": [
            "Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhao", 
        "givenName": "Bingyu", 
        "id": "sg:person.01362446505.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362446505.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Departments of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA", 
          "id": "http://www.grid.ac/institutes/grid.411017.2", 
          "name": [
            "Departments of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pereira", 
        "givenName": "Andy", 
        "id": "sg:person.01344430566.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344430566.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biological Sciences, University of North Texas, 76203, Denton, TX, USA", 
          "id": "http://www.grid.ac/institutes/grid.266869.5", 
          "name": [
            "Department of Biological Sciences, University of North Texas, 76203, Denton, TX, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shulaev", 
        "givenName": "Vladimir", 
        "id": "sg:person.011005623147.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005623147.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA", 
          "id": "http://www.grid.ac/institutes/grid.438526.e", 
          "name": [
            "Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Veilleux", 
        "givenName": "Richard E.", 
        "id": "sg:person.01236120527.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236120527.06"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1023/a:1006455130098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014682200", 
          "https://doi.org/10.1023/a:1006455130098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020375354", 
          "https://doi.org/10.1038/nature01218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-014-1610-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003575811", 
          "https://doi.org/10.1007/s00299-014-1610-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01214", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030621616", 
          "https://doi.org/10.1038/nature01214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00046092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047720531", 
          "https://doi.org/10.1007/bf00046092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00386991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031483970", 
          "https://doi.org/10.1007/bf00386991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-005-0193-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041705665", 
          "https://doi.org/10.1007/s00425-005-0193-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002525491", 
          "https://doi.org/10.1038/nature10158"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-08-12", 
    "datePublishedReg": "2014-08-12", 
    "description": "Transposon tagging is a powerful technology for studying gene function; however, somatic transposition prior to gametogenesis may result in multiple derivatives bearing the same insertion. In order to attempt to increase germinal transposition, we synthesized two novel transposon-tagging constructs. We placed the transposase gene under control of either of two microspore-specific promoters identified in Arabidopsis or Antirrhinum within the Ds element that also harbored kanamycin resistance. Both transposase and NPTII gene sequences were optimized in silico based on codon usage bias data in dicot species. Agrobacterium-mediated transformation was used to insert the constructs into diploid potato. The transgenic plants were subjected to both anther culture and outcrossing to wild type diploid potato to observe the opportunity for transposition in either gametophytic process. Green fluorescent protein screening was not efficient but kanamycin selection was able to eliminate 94\u00a0% of wild type progenies. Transposants were recovered following anther culture of two transgenic lines whereas no transposants were found among more than 250 T1 seedlings. Anther culture provided a unique venue for the observation of the influence of gametophye specific promoters on transposition in potato.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s11240-014-0576-3", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1123614", 
        "issn": [
          "0167-6857", 
          "1573-5044"
        ], 
        "name": "Plant Cell, Tissue and Organ Culture (PCTOC)", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "120"
      }
    ], 
    "keywords": [
      "anther culture", 
      "diploid potatoes", 
      "Agrobacterium-mediated transformation", 
      "microspore-specific promoters", 
      "transposable element movement", 
      "transgenic plants", 
      "dicot species", 
      "T1 seedlings", 
      "gene function", 
      "somatic transposition", 
      "gene sequences", 
      "kanamycin selection", 
      "transgenic lines", 
      "transposase gene", 
      "specific promoters", 
      "kanamycin resistance", 
      "type progeny", 
      "Ds element", 
      "same insertion", 
      "germinal transposition", 
      "protein screening", 
      "promoter", 
      "transposants", 
      "powerful technology", 
      "potato", 
      "element movement", 
      "Arabidopsis", 
      "Antirrhinum", 
      "transposon", 
      "gametogenesis", 
      "transposase", 
      "seedlings", 
      "genes", 
      "transposition", 
      "plants", 
      "progeny", 
      "species", 
      "culture", 
      "silico", 
      "sequence", 
      "constructs", 
      "multiple derivatives", 
      "insertion", 
      "selection", 
      "lines", 
      "function", 
      "resistance", 
      "unique venue", 
      "screening", 
      "elements", 
      "transformation", 
      "process", 
      "control", 
      "observations", 
      "movement", 
      "data", 
      "opportunities", 
      "derivatives", 
      "influence", 
      "order", 
      "technology", 
      "venues", 
      "bias data", 
      "novel transposon-tagging constructs", 
      "transposon-tagging constructs", 
      "NPTII gene sequences", 
      "codon usage bias data", 
      "usage bias data", 
      "wild type diploid potato", 
      "type diploid potato", 
      "gametophytic process", 
      "Green fluorescent protein screening", 
      "fluorescent protein screening", 
      "wild type progenies", 
      "gametophye specific promoters"
    ], 
    "name": "Anther culture induces transposable element movement in potato", 
    "pagination": "361-366", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1032319827"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11240-014-0576-3"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11240-014-0576-3", 
      "https://app.dimensions.ai/details/publication/pub.1032319827"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_645.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s11240-014-0576-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11240-014-0576-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11240-014-0576-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11240-014-0576-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11240-014-0576-3'


 

This table displays all metadata directly associated to this object as RDF triples.

199 TRIPLES      22 PREDICATES      108 URIs      92 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11240-014-0576-3 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nc5de327d82444335b9fff1eb461563f1
4 schema:citation sg:pub.10.1007/bf00046092
5 sg:pub.10.1007/bf00386991
6 sg:pub.10.1007/s00299-014-1610-y
7 sg:pub.10.1007/s00425-005-0193-9
8 sg:pub.10.1023/a:1006455130098
9 sg:pub.10.1038/nature01214
10 sg:pub.10.1038/nature01218
11 sg:pub.10.1038/nature10158
12 schema:datePublished 2014-08-12
13 schema:datePublishedReg 2014-08-12
14 schema:description Transposon tagging is a powerful technology for studying gene function; however, somatic transposition prior to gametogenesis may result in multiple derivatives bearing the same insertion. In order to attempt to increase germinal transposition, we synthesized two novel transposon-tagging constructs. We placed the transposase gene under control of either of two microspore-specific promoters identified in Arabidopsis or Antirrhinum within the Ds element that also harbored kanamycin resistance. Both transposase and NPTII gene sequences were optimized in silico based on codon usage bias data in dicot species. Agrobacterium-mediated transformation was used to insert the constructs into diploid potato. The transgenic plants were subjected to both anther culture and outcrossing to wild type diploid potato to observe the opportunity for transposition in either gametophytic process. Green fluorescent protein screening was not efficient but kanamycin selection was able to eliminate 94 % of wild type progenies. Transposants were recovered following anther culture of two transgenic lines whereas no transposants were found among more than 250 T1 seedlings. Anther culture provided a unique venue for the observation of the influence of gametophye specific promoters on transposition in potato.
15 schema:genre article
16 schema:inLanguage en
17 schema:isAccessibleForFree false
18 schema:isPartOf N7a6b7b45f5964b209da188e04f1c0b29
19 Nb72ebe53c804428ba629878fd8ab6adf
20 sg:journal.1123614
21 schema:keywords Agrobacterium-mediated transformation
22 Antirrhinum
23 Arabidopsis
24 Ds element
25 Green fluorescent protein screening
26 NPTII gene sequences
27 T1 seedlings
28 anther culture
29 bias data
30 codon usage bias data
31 constructs
32 control
33 culture
34 data
35 derivatives
36 dicot species
37 diploid potatoes
38 element movement
39 elements
40 fluorescent protein screening
41 function
42 gametogenesis
43 gametophye specific promoters
44 gametophytic process
45 gene function
46 gene sequences
47 genes
48 germinal transposition
49 influence
50 insertion
51 kanamycin resistance
52 kanamycin selection
53 lines
54 microspore-specific promoters
55 movement
56 multiple derivatives
57 novel transposon-tagging constructs
58 observations
59 opportunities
60 order
61 plants
62 potato
63 powerful technology
64 process
65 progeny
66 promoter
67 protein screening
68 resistance
69 same insertion
70 screening
71 seedlings
72 selection
73 sequence
74 silico
75 somatic transposition
76 species
77 specific promoters
78 technology
79 transformation
80 transgenic lines
81 transgenic plants
82 transposable element movement
83 transposants
84 transposase
85 transposase gene
86 transposition
87 transposon
88 transposon-tagging constructs
89 type diploid potato
90 type progeny
91 unique venue
92 usage bias data
93 venues
94 wild type diploid potato
95 wild type progenies
96 schema:name Anther culture induces transposable element movement in potato
97 schema:pagination 361-366
98 schema:productId N6d8e43dc2e3c45e6bc798dcfb24b3703
99 N8c52b934d4864810b9a58c2b72b2bff0
100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032319827
101 https://doi.org/10.1007/s11240-014-0576-3
102 schema:sdDatePublished 2021-12-01T19:32
103 schema:sdLicense https://scigraph.springernature.com/explorer/license/
104 schema:sdPublisher Ne5aeeba921d04128909e4b8e1189f6fe
105 schema:url https://doi.org/10.1007/s11240-014-0576-3
106 sgo:license sg:explorer/license/
107 sgo:sdDataset articles
108 rdf:type schema:ScholarlyArticle
109 N0736dc3718e84b4e9459e5bf11bc8968 rdf:first sg:person.01362446505.23
110 rdf:rest N4bb4ba707ecf45a78059ac206bdd77a8
111 N4bb4ba707ecf45a78059ac206bdd77a8 rdf:first sg:person.01344430566.76
112 rdf:rest Nd786ba7684cf423585289c52ec5e0a70
113 N6d8e43dc2e3c45e6bc798dcfb24b3703 schema:name doi
114 schema:value 10.1007/s11240-014-0576-3
115 rdf:type schema:PropertyValue
116 N7a6b7b45f5964b209da188e04f1c0b29 schema:issueNumber 1
117 rdf:type schema:PublicationIssue
118 N8c52b934d4864810b9a58c2b72b2bff0 schema:name dimensions_id
119 schema:value pub.1032319827
120 rdf:type schema:PropertyValue
121 Nb72ebe53c804428ba629878fd8ab6adf schema:volumeNumber 120
122 rdf:type schema:PublicationVolume
123 Nc5c9ea39b69d4c429930adfe070d5e0e rdf:first sg:person.01236120527.06
124 rdf:rest rdf:nil
125 Nc5de327d82444335b9fff1eb461563f1 rdf:first sg:person.01011304627.85
126 rdf:rest N0736dc3718e84b4e9459e5bf11bc8968
127 Nd786ba7684cf423585289c52ec5e0a70 rdf:first sg:person.011005623147.69
128 rdf:rest Nc5c9ea39b69d4c429930adfe070d5e0e
129 Ne5aeeba921d04128909e4b8e1189f6fe schema:name Springer Nature - SN SciGraph project
130 rdf:type schema:Organization
131 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
132 schema:name Biological Sciences
133 rdf:type schema:DefinedTerm
134 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
135 schema:name Genetics
136 rdf:type schema:DefinedTerm
137 sg:journal.1123614 schema:issn 0167-6857
138 1573-5044
139 schema:name Plant Cell, Tissue and Organ Culture (PCTOC)
140 schema:publisher Springer Nature
141 rdf:type schema:Periodical
142 sg:person.01011304627.85 schema:affiliation grid-institutes:grid.438526.e
143 schema:familyName Lu
144 schema:givenName Nan
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011304627.85
146 rdf:type schema:Person
147 sg:person.011005623147.69 schema:affiliation grid-institutes:grid.266869.5
148 schema:familyName Shulaev
149 schema:givenName Vladimir
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005623147.69
151 rdf:type schema:Person
152 sg:person.01236120527.06 schema:affiliation grid-institutes:grid.438526.e
153 schema:familyName Veilleux
154 schema:givenName Richard E.
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236120527.06
156 rdf:type schema:Person
157 sg:person.01344430566.76 schema:affiliation grid-institutes:grid.411017.2
158 schema:familyName Pereira
159 schema:givenName Andy
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344430566.76
161 rdf:type schema:Person
162 sg:person.01362446505.23 schema:affiliation grid-institutes:grid.438526.e
163 schema:familyName Zhao
164 schema:givenName Bingyu
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362446505.23
166 rdf:type schema:Person
167 sg:pub.10.1007/bf00046092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047720531
168 https://doi.org/10.1007/bf00046092
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/bf00386991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031483970
171 https://doi.org/10.1007/bf00386991
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s00299-014-1610-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1003575811
174 https://doi.org/10.1007/s00299-014-1610-y
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s00425-005-0193-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041705665
177 https://doi.org/10.1007/s00425-005-0193-9
178 rdf:type schema:CreativeWork
179 sg:pub.10.1023/a:1006455130098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014682200
180 https://doi.org/10.1023/a:1006455130098
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nature01214 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030621616
183 https://doi.org/10.1038/nature01214
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/nature01218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020375354
186 https://doi.org/10.1038/nature01218
187 rdf:type schema:CreativeWork
188 sg:pub.10.1038/nature10158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002525491
189 https://doi.org/10.1038/nature10158
190 rdf:type schema:CreativeWork
191 grid-institutes:grid.266869.5 schema:alternateName Department of Biological Sciences, University of North Texas, 76203, Denton, TX, USA
192 schema:name Department of Biological Sciences, University of North Texas, 76203, Denton, TX, USA
193 rdf:type schema:Organization
194 grid-institutes:grid.411017.2 schema:alternateName Departments of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA
195 schema:name Departments of Crop, Soil and Environmental Sciences, University of Arkansas, 72701, Fayetteville, AR, USA
196 rdf:type schema:Organization
197 grid-institutes:grid.438526.e schema:alternateName Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA
198 schema:name Department of Horticulture, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA
199 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...