Pyramiding of Bt cry1Ia8 and cry1Ba3 genes into cabbage (Brassica oleracea L. var. capitata) confers effective control against diamondback moth View Full Text


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Article Info

DATE

2013-08-23

AUTHORS

Dengxia Yi, Lei Cui, Li Wang, Yumei Liu, Mu Zhuang, Yangyong Zhang, Jie Zhang, Zhihong Lang, Zhenxian Zhang, Zhiyuan Fang, Limei Yang

ABSTRACT

A binary vector carrying Bacillus thuringiensiscry1Ia8 and cry1Ba3 genes was introduced into an inbred line of white cabbage by Agrobacterium tumefaciens-mediated transformation for control of diamondback moth (DBM, Plutella xylostella), and 14 kanamycin-resistant plantlets were obtained. Presence and expression of the cry1Ia8 and cry1Ba3 genes in five transformed plants were confirmed by polymerase chain reaction (PCR), Southern blot, reverse transcription-polymerase chain reaction (RT-PCR), and Western blot analyses. Insect bioassays showed that these transgenic plants were able to effectively control both susceptible and Cry1Ac-resistant DBM larvae as compared to non-transformed counterparts. Ten homozygous insect-resistant cabbage lines were obtained in the T2 generation through self-pollination, and molecular methods and insect bioassays. After natural infestation under greenhouse and field conditions, the pyramided lines exhibited excellent efficacy against DBM. Furthermore, data from field trials indicated that there were no significant differences in most agronomic traits between most the homozygous lines and the original variety. These transgenic lines may allow field study of resistance management strategies involving gene pyramiding and serve as novel insect-resistant resources in cabbage breeding. More... »

PAGES

419-428

References to SciGraph publications

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  • 2002-06-14. Broccoli plants with pyramided cry1Ac and cry1C Bt genes control diamondback moths resistant to Cry1A and Cry1C proteins in THEORETICAL AND APPLIED GENETICS
  • 2000-07. Development of transgenic cabbage (Brassica oleracea var. Capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2003-11-09. Transgenic plants expressing two Bacillus thuringiensis toxins delay insect resistance evolution in NATURE BIOTECHNOLOGY
  • 1993-08-20. Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence in BIOMEMBRANE PROTOCOLS
  • 2010-12-15. Transgenic Brassica napus L. lines carrying a two gene construct demonstrate enhanced resistance against Plutella xylostella and Sclerotinia sclerotiorum in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2000-10. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin in NATURE BIOTECHNOLOGY
  • 1995-12-01. Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata) in PLANT CELL REPORTS
  • 2008. How Governmental Regulation Can Help or Hinder the Integration of Bt Crops within IPM Programs in INTEGRATION OF INSECT-RESISTANT GENETICALLY MODIFIED CROPS WITHIN IPM PROGRAMS
  • 2007-11-08. Sequential transformation to pyramid two Bt genes in vegetable Indian mustard (Brassica juncea L.) and its potential for control of diamondback moth larvae in PLANT CELL REPORTS
  • 2005-01-01. Insect resistance management in GM crops: past, present and future in NATURE BIOTECHNOLOGY
  • 2012-10-05. Comparative Persistence of Thiacloprid in Bt-Transgenic Cabbage (Brassica oleracea cv. capitata) vis-à-vis Non-Transgenic Crop and its Decontamination in BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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