Ontology type: schema:ScholarlyArticle
2005-10
AUTHORSMasoud Tohidfar, Mojtaba Mohammadi, Behzad Ghareyazie
ABSTRACTCotton (Gossypium hirsutum L., var. Coker 312) hypocotyl explants were transformed with three strains of Agrobacterium tumefaciens, LBA4404, EHA101 and C58, each harboring the recombinant binary vector pBI121 containing the chi gene insert and neomycin phosphotransferase (nptII) gene, as selectable marker. Inoculated tissue sections were placed onto cotton co-cultivation medium. Transformed calli were selected on MS medium containing 50 mg l−1 kanamycin and 200 mg l−1 cepotaxime. Putative calli were subsequently regenerated into cotton plantlets expressing both the kanamycin resistance gene and βglucuronidase (gus) as a reporter gene. Polymerase chain reaction was used to confirm the integration of chi and nptII transgenes in the T1 plants genome. Integration of chi gene into the genome of putative transgenic was further confirmed by Southern blot analysis. ‘Western’ immunoblot analysis of leaves isolated from T0 transformants and progeny plants (T1) revealed the presence of an immunoreactive band with MW of approximately 31 kDa in transgenic cotton lines using anti-chitinase-I polyclonal anti-serum. Untransformed control and one transgenic line did not show such an immunoreactive band. Chitinase specific activity in leaf tissues of transgenic lines was several folds greater than that of untransformed cotton. Crude leaf extracts from transgenic lines showed in vitro inhibitory activity against Verticillium dahliae.Transgenic plants currently growing in a greenhouse and will be bioassayed for improved resistance against V. dahlia the causal against of verticilliosis in cotton. More... »
PAGES83-96
http://scigraph.springernature.com/pub.10.1007/s11240-004-6155-2
DOIhttp://dx.doi.org/10.1007/s11240-004-6155-2
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1018263499
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Plant Biology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Agricultural Biotechnology, Research Institute of Iran (ABRII), M\u00e2hdasht Road, P.O. Box v, Karaj, Iran",
"id": "http://www.grid.ac/institutes/grid.417749.8",
"name": [
"Agricultural Biotechnology, Research Institute of Iran (ABRII), M\u00e2hdasht Road, P.O. Box v, Karaj, Iran"
],
"type": "Organization"
},
"familyName": "Tohidfar",
"givenName": "Masoud",
"id": "sg:person.01126177703.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126177703.58"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Plant Pathology Entomology College of Agriculture, University of Tehran, 31587-11167, Karaj, Iran",
"id": "http://www.grid.ac/institutes/grid.46072.37",
"name": [
"Department of Plant Pathology Entomology College of Agriculture, University of Tehran, 31587-11167, Karaj, Iran"
],
"type": "Organization"
},
"familyName": "Mohammadi",
"givenName": "Mojtaba",
"id": "sg:person.012672616142.28",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012672616142.28"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Agricultural Biotechnology, Research Institute of Iran (ABRII), M\u00e2hdasht Road, P.O. Box v, Karaj, Iran",
"id": "http://www.grid.ac/institutes/grid.417749.8",
"name": [
"Agricultural Biotechnology, Research Institute of Iran (ABRII), M\u00e2hdasht Road, P.O. Box v, Karaj, Iran"
],
"type": "Organization"
},
"familyName": "Ghareyazie",
"givenName": "Behzad",
"id": "sg:person.0754425644.60",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754425644.60"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s001220051248",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019011249",
"https://doi.org/10.1007/s001220051248"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00394536",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024658349",
"https://doi.org/10.1007/bf00394536"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002990050759",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029499433",
"https://doi.org/10.1007/s002990050759"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0387-263",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032761222",
"https://doi.org/10.1038/nbt0387-263"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00016148",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039226496",
"https://doi.org/10.1007/bf00016148"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00232216",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044260010",
"https://doi.org/10.1007/bf00232216"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00033739",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041928350",
"https://doi.org/10.1007/bf00033739"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0795-686",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018917840",
"https://doi.org/10.1038/nbt0795-686"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s001220051065",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051959117",
"https://doi.org/10.1007/s001220051065"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00232459",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047168538",
"https://doi.org/10.1007/bf00232459"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s002999900174",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029266056",
"https://doi.org/10.1007/s002999900174"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02772162",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031012052",
"https://doi.org/10.1007/bf02772162"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/227680a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010419937",
"https://doi.org/10.1038/227680a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf01092762",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010799304",
"https://doi.org/10.1007/bf01092762"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.jim.2900447",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000094333",
"https://doi.org/10.1038/sj.jim.2900447"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/324365a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013176934",
"https://doi.org/10.1038/324365a0"
],
"type": "CreativeWork"
}
],
"datePublished": "2005-10",
"datePublishedReg": "2005-10-01",
"description": "Cotton (Gossypium hirsutum L., var. Coker 312) hypocotyl explants were transformed with three strains of Agrobacterium tumefaciens, LBA4404, EHA101 and C58, each harboring the recombinant binary vector pBI121 containing the chi gene insert and neomycin phosphotransferase (nptII) gene, as selectable marker. Inoculated tissue sections were placed onto cotton co-cultivation medium. Transformed calli were selected on MS medium containing 50\u00a0mg l\u22121 kanamycin and 200\u00a0mg l\u22121 cepotaxime. Putative calli were subsequently regenerated into cotton plantlets expressing both the kanamycin resistance gene and \u03b2glucuronidase (gus) as a reporter gene. Polymerase chain reaction was used to confirm the integration of chi and nptII transgenes in the T1 plants genome. Integration of chi gene into the genome of putative transgenic was further confirmed by Southern blot analysis. \u2018Western\u2019 immunoblot analysis of leaves isolated from T0 transformants and progeny plants (T1) revealed the presence of an immunoreactive band with MW of approximately 31\u00a0kDa in transgenic cotton lines using anti-chitinase-I polyclonal anti-serum. Untransformed control and one transgenic line did not show such an immunoreactive band. Chitinase specific activity in leaf tissues of transgenic lines was several folds greater than that of untransformed cotton. Crude leaf extracts from transgenic lines showed in vitro inhibitory activity against Verticillium dahliae.Transgenic plants currently growing in a greenhouse and will be bioassayed for improved resistance against V. dahlia the causal against of verticilliosis in cotton.",
"genre": "article",
"id": "sg:pub.10.1007/s11240-004-6155-2",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1123614",
"issn": [
"0167-6857",
"1573-5044"
],
"name": "Plant Cell, Tissue and Organ Culture (PCTOC)",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "83"
}
],
"keywords": [
"transgenic lines",
"recombinant binary vector",
"transgenic cotton lines",
"bean chitinase gene",
"Agrobacterium-mediated transformation",
"kanamycin resistance gene",
"Southern blot analysis",
"co-cultivation medium",
"transgenic plants",
"T1 plants",
"progeny plants",
"immunoreactive bands",
"cotton plantlets",
"putative transgenics",
"chitinase specific activity",
"untransformed controls",
"chitinase gene",
"CHI gene",
"cotton lines",
"Agrobacterium tumefaciens",
"leaf tissue",
"selectable marker",
"reporter gene",
"Verticillium dahliae",
"nptII transgene",
"V. dahlia",
"hypocotyl explants",
"T0 transformants",
"resistance genes",
"binary vector",
"genes",
"MS medium",
"gene insert",
"immunoblot analysis",
"crude leaf extracts",
"blot analysis",
"plants",
"callus",
"cotton",
"specific activity",
"improved resistance",
"polymerase chain reaction",
"genome",
"tumefaciens",
"LBA4404",
"EHA101",
"transformants",
"dahliae",
"chain reaction",
"plantlets",
"leaf extract",
"leaves",
"C58",
"transgene",
"transgenic",
"lines",
"greenhouse",
"inhibitory activity",
"dahlia",
"explants",
"tissue sections",
"activity",
"band",
"kanamycin",
"strains",
"folds",
"neomycin",
"inserts",
"markers",
"tissue",
"integration",
"vector",
"extract",
"medium",
"analysis",
"resistance",
"mW",
"presence",
"causal",
"control",
"transformation",
"reaction",
"Chi",
"sections"
],
"name": "Agrobacterium -mediated transformation of cotton (Gossypium hirsutum) using a heterologous bean chitinase gene",
"pagination": "83-96",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1018263499"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11240-004-6155-2"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11240-004-6155-2",
"https://app.dimensions.ai/details/publication/pub.1018263499"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:55",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_406.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s11240-004-6155-2"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11240-004-6155-2'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11240-004-6155-2'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11240-004-6155-2'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11240-004-6155-2'
This table displays all metadata directly associated to this object as RDF triples.
222 TRIPLES
21 PREDICATES
125 URIs
101 LITERALS
6 BLANK NODES