Immobilization of chymotrypsin on silver nanoparticles View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-03

AUTHORS

O. A. Pisarev, A. V. Titova, L. N. Borovikova, A. I. Kipper, T. M. Voroshilova, E. F. Panarin

ABSTRACT

The method of immobilization of chymotrypsin based on the reduction reaction of silver nitrate with sodium borohydride in an aqueous medium in the presence of the enzyme was developed. Chymotrypsin was simultaneously a stabilizer of silver nanoparticles in solution. The dimensional characteristics, as well as proteolytic and bactericidal activity of the synthesized nanocomposites were studied. It is shown that the hybrid organic-inorganic nanocomposites based on chymotrypsin and silver nanoparticles are able to perform an increased proteolytic activity in comparison with the native enzyme in the pH range corresponding to wound surface, and suppress pathogenic flora of various microorganisms. More... »

PAGES

790-793

Journal

TITLE

Russian Chemical Bulletin

ISSUE

3

VOLUME

65

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11172-016-1375-2

DOI

http://dx.doi.org/10.1007/s11172-016-1375-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024030979


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