The PshX subunit of the photochemical reaction center from Heliobacterium modesticaldum acts as a low-energy antenna View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2021-09-04

AUTHORS

Gregory S. Orf, Christopher J. Gisriel, Jesse Granstrom, Patricia L. Baker, Kevin E. Redding

ABSTRACT

The anoxygenic phototrophic bacterium Heliobacterium modesticaldum contains a photochemical reaction center protein complex (called the HbRC) consisting of a homodimer of the PshA polypeptide and two copies of a newly discovered polypeptide called PshX, which is a single transmembrane helix that binds two bacteriochlorophyll g molecules. To assess the function of PshX, we produced a ∆pshX strain of Hbt. modesticaldum by leveraging the endogenous Hbt. modesticaldum Type I-A CRISPR-Cas system to aid in mutant selection. We optimized this system by separating the homologous recombination and CRISPR-based selection steps into two plasmid transformations, allowing for markerless gene replacement. Fluorescence and low-temperature absorbance of the purified HbRC from the wild-type and ∆pshX strains showed that the bacteriochlorophylls bound by PshX have the lowest site energies in the entire HbRC. This indicates that PshX acts as a low-energy antenna subunit, participating in entropy-assisted uphill energy transfer toward the P800 special bacteriochlorophyll g pair. We further discuss the role that PshX may play in stability of the HbRC, its conservation in other heliobacterial species, and the evolutionary pressure to produce and maintain single-TMH subunits in similar locations in other reaction centers. More... »

PAGES

11-30

References to SciGraph publications

  • 2018-03-30. Evolution of photosynthetic reaction centers: insights from the structure of the heliobacterial reaction center in PHOTOSYNTHESIS RESEARCH
  • 2012-03-02. Purification of the photosynthetic reaction center from Heliobacterium modesticaldum in PHOTOSYNTHESIS RESEARCH
  • 2006-11-22. Silver staining of proteins in polyacrylamide gels in NATURE PROTOCOLS
  • 2017-10-31. Computational Analysis of Protein Tunnels and Channels in PROTEIN ENGINEERING
  • 2019-08-02. Red-shifted light-harvesting system of freshwater eukaryotic alga Trachydiscus minutus (Eustigmatophyta, Stramenopila) in PHOTOSYNTHESIS RESEARCH
  • 2018-07-23. Photosynthesis supported by a chlorophyll f-dependent, entropy-driven uphill energy transfer in Halomicronema hongdechloris cells adapted to far-red light in PHOTOSYNTHESIS RESEARCH
  • 2018-03-12. Light-driven quinone reduction in heliobacterial membranes in PHOTOSYNTHESIS RESEARCH
  • 1995-04. Heliobacterium modesticaldum, sp. nov., a thermophilic heliobacterium of hot springs and volcanic soils in ARCHIVES OF MICROBIOLOGY
  • 2014-12-16. A fresh look at the evolution and diversification of photochemical reaction centers in PHOTOSYNTHESIS RESEARCH
  • 2013-06-30. Temporal and spectral characterization of the photosynthetic reaction center from Heliobacterium modesticaldum in PHOTOSYNTHESIS RESEARCH
  • 2010-01-22. Taxonomy, phylogeny, and ecology of the heliobacteria in PHOTOSYNTHESIS RESEARCH
  • 2010-02-04. Insights into heliobacterial photosynthesis and physiology from the genome of Heliobacterium modesticaldum in PHOTOSYNTHESIS RESEARCH
  • 2006-01-01. The Long Wavelength Chlorophylls of Photosystem I in PHOTOSYSTEM I
  • 2020-01-13. Structural basis for the adaptation and function of chlorophyll f in photosystem I in NATURE COMMUNICATIONS
  • 2001-06. Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution in NATURE
  • 2006-05-12. Tricine–SDS-PAGE in NATURE PROTOCOLS
  • 2008-01-30. Protein production and purification in NATURE METHODS
  • 2016-05-09. Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium in SCIENTIFIC REPORTS
  • 2019-09-21. Expression and purification of affinity-tagged variants of the photochemical reaction center from Heliobacterium modesticaldum in PHOTOSYNTHESIS RESEARCH
  • 2021-05-14. Excitonic structure and charge separation in the heliobacterial reaction center probed by multispectral multidimensional spectroscopy in NATURE COMMUNICATIONS
  • 2017-05-25. Characterization of a newly isolated freshwater Eustigmatophyte alga capable of utilizing far-red light as its sole light source in PHOTOSYNTHESIS RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11120-021-00871-x

    DOI

    http://dx.doi.org/10.1007/s11120-021-00871-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1140869462

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34480322


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteriochlorophylls", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Clostridiales", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Infectious Disease Research, Abbott Diagnostics Division, 60064, Abbott Park, IL, USA", 
              "id": "http://www.grid.ac/institutes/grid.417574.4", 
              "name": [
                "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA", 
                "Infectious Disease Research, Abbott Diagnostics Division, 60064, Abbott Park, IL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Orf", 
            "givenName": "Gregory S.", 
            "id": "sg:person.0630653007.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630653007.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Chemistry, Yale University, 06520, New Haven, CT, USA", 
              "id": "http://www.grid.ac/institutes/grid.47100.32", 
              "name": [
                "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA", 
                "Department of Chemistry, Yale University, 06520, New Haven, CT, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gisriel", 
            "givenName": "Christopher  J.", 
            "id": "sg:person.01102443555.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102443555.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA", 
              "id": "http://www.grid.ac/institutes/grid.215654.1", 
              "name": [
                "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Granstrom", 
            "givenName": "Jesse", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA", 
              "id": "http://www.grid.ac/institutes/grid.215654.1", 
              "name": [
                "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Baker", 
            "givenName": "Patricia  L.", 
            "id": "sg:person.01042026314.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042026314.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA", 
              "id": "http://www.grid.ac/institutes/grid.215654.1", 
              "name": [
                "Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Redding", 
            "givenName": "Kevin E.", 
            "id": "sg:person.01272503314.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272503314.34"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s11120-017-0401-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085601724", 
              "https://doi.org/10.1007/s11120-017-0401-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-018-0556-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105775703", 
              "https://doi.org/10.1007/s11120-018-0556-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.f.202", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035202423", 
              "https://doi.org/10.1038/nmeth.f.202"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4939-7366-8_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092443730", 
              "https://doi.org/10.1007/978-1-4939-7366-8_3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-012-9726-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049636598", 
              "https://doi.org/10.1007/s11120-012-9726-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4020-4256-0_13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044688748", 
              "https://doi.org/10.1007/978-1-4020-4256-0_13"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2006.288", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053697239", 
              "https://doi.org/10.1038/nprot.2006.288"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-009-9516-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006689152", 
              "https://doi.org/10.1007/s11120-009-9516-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00393378", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021995899", 
              "https://doi.org/10.1007/bf00393378"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-018-0503-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101844443", 
              "https://doi.org/10.1007/s11120-018-0503-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-018-0496-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101509061", 
              "https://doi.org/10.1007/s11120-018-0496-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35082000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030227276", 
              "https://doi.org/10.1038/35082000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-019-00672-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1121175874", 
              "https://doi.org/10.1007/s11120-019-00672-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep25666", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051165506", 
              "https://doi.org/10.1038/srep25666"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-013-9871-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047180540", 
              "https://doi.org/10.1007/s11120-013-9871-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-014-0065-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015767636", 
              "https://doi.org/10.1007/s11120-014-0065-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-019-00662-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1120095953", 
              "https://doi.org/10.1007/s11120-019-00662-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2006.4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031570865", 
              "https://doi.org/10.1038/nprot.2006.4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-019-13898-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1124047941", 
              "https://doi.org/10.1038/s41467-019-13898-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-021-23060-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1138064085", 
              "https://doi.org/10.1038/s41467-021-23060-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11120-010-9529-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018996460", 
              "https://doi.org/10.1007/s11120-010-9529-9"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2021-09-04", 
        "datePublishedReg": "2021-09-04", 
        "description": "The anoxygenic phototrophic bacterium Heliobacterium modesticaldum contains a photochemical reaction center protein complex (called the HbRC) consisting of a homodimer of the PshA polypeptide and two copies of a newly discovered polypeptide called PshX, which is a single transmembrane helix that binds two bacteriochlorophyll g molecules. To assess the function of PshX, we produced a \u2206pshX strain of Hbt. modesticaldum by leveraging the endogenous Hbt. modesticaldum Type I-A CRISPR-Cas system to aid in mutant selection. We optimized this system by separating the homologous recombination and CRISPR-based selection steps into two plasmid transformations, allowing for markerless gene replacement. Fluorescence and low-temperature absorbance of the purified HbRC from the wild-type and \u2206pshX strains showed that the bacteriochlorophylls bound by PshX have the lowest site energies in the entire HbRC. This indicates that PshX acts as a low-energy antenna subunit, participating in entropy-assisted uphill energy transfer toward the P800 special bacteriochlorophyll g pair. We further discuss the role that PshX may play in stability of the HbRC, its conservation in other heliobacterial species, and the evolutionary pressure to produce and maintain single-TMH subunits in similar locations in other reaction centers.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s11120-021-00871-x", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.4322241", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1022986", 
            "issn": [
              "0166-8595", 
              "1573-5079"
            ], 
            "name": "Photosynthesis Research", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "151"
          }
        ], 
        "keywords": [
          "single transmembrane helix", 
          "reaction center protein complex", 
          "CRISPR-Cas systems", 
          "Heliobacterium modesticaldum", 
          "photochemical reaction center", 
          "transmembrane helices", 
          "reaction centers", 
          "protein complexes", 
          "low-temperature absorbance", 
          "markerless gene replacement", 
          "evolutionary pressure", 
          "homologous recombination", 
          "gene replacement", 
          "antenna subunits", 
          "mutant selection", 
          "subunits", 
          "plasmid transformation", 
          "bacteriochlorophyll", 
          "polypeptide", 
          "uphill energy transfer", 
          "similar locations", 
          "selection step", 
          "CRISPR", 
          "homodimer", 
          "type I", 
          "lowest site energy", 
          "species", 
          "strains", 
          "helix", 
          "conservation", 
          "copies", 
          "recombination", 
          "complexes", 
          "HbRC", 
          "fluorescence", 
          "molecules", 
          "energy transfer", 
          "role", 
          "selection", 
          "function", 
          "pairs", 
          "step", 
          "location", 
          "replacement", 
          "transfer", 
          "system", 
          "transformation", 
          "stability", 
          "absorbance", 
          "center", 
          "site energies", 
          "pressure", 
          "antenna", 
          "energy", 
          "HBTs"
        ], 
        "name": "The PshX subunit of the photochemical reaction center from Heliobacterium modesticaldum acts as a low-energy antenna", 
        "pagination": "11-30", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1140869462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11120-021-00871-x"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "34480322"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11120-021-00871-x", 
          "https://app.dimensions.ai/details/publication/pub.1140869462"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-06-01T22:22", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_894.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s11120-021-00871-x"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11120-021-00871-x'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11120-021-00871-x'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11120-021-00871-x'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11120-021-00871-x'


     

    This table displays all metadata directly associated to this object as RDF triples.

    246 TRIPLES      22 PREDICATES      104 URIs      75 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11120-021-00871-x schema:about N3f4e41996e0642b497a117d57a347eae
    2 N9b85645e72c74c25ba2c7f0ff721284e
    3 anzsrc-for:06
    4 anzsrc-for:0604
    5 schema:author Nc6a72e5035914037a649a379df6fcd08
    6 schema:citation sg:pub.10.1007/978-1-4020-4256-0_13
    7 sg:pub.10.1007/978-1-4939-7366-8_3
    8 sg:pub.10.1007/bf00393378
    9 sg:pub.10.1007/s11120-009-9516-1
    10 sg:pub.10.1007/s11120-010-9529-9
    11 sg:pub.10.1007/s11120-012-9726-9
    12 sg:pub.10.1007/s11120-013-9871-9
    13 sg:pub.10.1007/s11120-014-0065-x
    14 sg:pub.10.1007/s11120-017-0401-z
    15 sg:pub.10.1007/s11120-018-0496-x
    16 sg:pub.10.1007/s11120-018-0503-2
    17 sg:pub.10.1007/s11120-018-0556-2
    18 sg:pub.10.1007/s11120-019-00662-5
    19 sg:pub.10.1007/s11120-019-00672-3
    20 sg:pub.10.1038/35082000
    21 sg:pub.10.1038/nmeth.f.202
    22 sg:pub.10.1038/nprot.2006.288
    23 sg:pub.10.1038/nprot.2006.4
    24 sg:pub.10.1038/s41467-019-13898-5
    25 sg:pub.10.1038/s41467-021-23060-9
    26 sg:pub.10.1038/srep25666
    27 schema:datePublished 2021-09-04
    28 schema:datePublishedReg 2021-09-04
    29 schema:description The anoxygenic phototrophic bacterium Heliobacterium modesticaldum contains a photochemical reaction center protein complex (called the HbRC) consisting of a homodimer of the PshA polypeptide and two copies of a newly discovered polypeptide called PshX, which is a single transmembrane helix that binds two bacteriochlorophyll g molecules. To assess the function of PshX, we produced a ∆pshX strain of Hbt. modesticaldum by leveraging the endogenous Hbt. modesticaldum Type I-A CRISPR-Cas system to aid in mutant selection. We optimized this system by separating the homologous recombination and CRISPR-based selection steps into two plasmid transformations, allowing for markerless gene replacement. Fluorescence and low-temperature absorbance of the purified HbRC from the wild-type and ∆pshX strains showed that the bacteriochlorophylls bound by PshX have the lowest site energies in the entire HbRC. This indicates that PshX acts as a low-energy antenna subunit, participating in entropy-assisted uphill energy transfer toward the P800 special bacteriochlorophyll g pair. We further discuss the role that PshX may play in stability of the HbRC, its conservation in other heliobacterial species, and the evolutionary pressure to produce and maintain single-TMH subunits in similar locations in other reaction centers.
    30 schema:genre article
    31 schema:inLanguage en
    32 schema:isAccessibleForFree false
    33 schema:isPartOf N1fd3fb93c9684d9b8c3c7298e753cbc7
    34 N679baa4ca542404d938605dca206356e
    35 sg:journal.1022986
    36 schema:keywords CRISPR
    37 CRISPR-Cas systems
    38 HBTs
    39 HbRC
    40 Heliobacterium modesticaldum
    41 absorbance
    42 antenna
    43 antenna subunits
    44 bacteriochlorophyll
    45 center
    46 complexes
    47 conservation
    48 copies
    49 energy
    50 energy transfer
    51 evolutionary pressure
    52 fluorescence
    53 function
    54 gene replacement
    55 helix
    56 homodimer
    57 homologous recombination
    58 location
    59 low-temperature absorbance
    60 lowest site energy
    61 markerless gene replacement
    62 molecules
    63 mutant selection
    64 pairs
    65 photochemical reaction center
    66 plasmid transformation
    67 polypeptide
    68 pressure
    69 protein complexes
    70 reaction center protein complex
    71 reaction centers
    72 recombination
    73 replacement
    74 role
    75 selection
    76 selection step
    77 similar locations
    78 single transmembrane helix
    79 site energies
    80 species
    81 stability
    82 step
    83 strains
    84 subunits
    85 system
    86 transfer
    87 transformation
    88 transmembrane helices
    89 type I
    90 uphill energy transfer
    91 schema:name The PshX subunit of the photochemical reaction center from Heliobacterium modesticaldum acts as a low-energy antenna
    92 schema:pagination 11-30
    93 schema:productId N4e92dcde00ce4c338128de2730d583ea
    94 N8ac596e449754bf099d911f46809bde8
    95 Nd4d4370c5cfd4e1d81a6b85837e51504
    96 schema:sameAs https://app.dimensions.ai/details/publication/pub.1140869462
    97 https://doi.org/10.1007/s11120-021-00871-x
    98 schema:sdDatePublished 2022-06-01T22:22
    99 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    100 schema:sdPublisher Nf62ac14c0f064e78a0d5ed6dd2a75a78
    101 schema:url https://doi.org/10.1007/s11120-021-00871-x
    102 sgo:license sg:explorer/license/
    103 sgo:sdDataset articles
    104 rdf:type schema:ScholarlyArticle
    105 N00db893938a744a0857871680a502100 rdf:first sg:person.01042026314.63
    106 rdf:rest N45372ad5edd046578f1c031d13223185
    107 N0aa20ce269ed49edac0c092ed274b889 schema:affiliation grid-institutes:grid.215654.1
    108 schema:familyName Granstrom
    109 schema:givenName Jesse
    110 rdf:type schema:Person
    111 N1fd3fb93c9684d9b8c3c7298e753cbc7 schema:issueNumber 1
    112 rdf:type schema:PublicationIssue
    113 N3f4e41996e0642b497a117d57a347eae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Bacteriochlorophylls
    115 rdf:type schema:DefinedTerm
    116 N45372ad5edd046578f1c031d13223185 rdf:first sg:person.01272503314.34
    117 rdf:rest rdf:nil
    118 N4e92dcde00ce4c338128de2730d583ea schema:name dimensions_id
    119 schema:value pub.1140869462
    120 rdf:type schema:PropertyValue
    121 N679baa4ca542404d938605dca206356e schema:volumeNumber 151
    122 rdf:type schema:PublicationVolume
    123 N8ac596e449754bf099d911f46809bde8 schema:name doi
    124 schema:value 10.1007/s11120-021-00871-x
    125 rdf:type schema:PropertyValue
    126 N9b85645e72c74c25ba2c7f0ff721284e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Clostridiales
    128 rdf:type schema:DefinedTerm
    129 Nc1cc916f47aa459f8fcf7a00f40a98d9 rdf:first sg:person.01102443555.03
    130 rdf:rest Nc3562a68e04d48f7bd5faa7153e7eaa8
    131 Nc3562a68e04d48f7bd5faa7153e7eaa8 rdf:first N0aa20ce269ed49edac0c092ed274b889
    132 rdf:rest N00db893938a744a0857871680a502100
    133 Nc6a72e5035914037a649a379df6fcd08 rdf:first sg:person.0630653007.39
    134 rdf:rest Nc1cc916f47aa459f8fcf7a00f40a98d9
    135 Nd4d4370c5cfd4e1d81a6b85837e51504 schema:name pubmed_id
    136 schema:value 34480322
    137 rdf:type schema:PropertyValue
    138 Nf62ac14c0f064e78a0d5ed6dd2a75a78 schema:name Springer Nature - SN SciGraph project
    139 rdf:type schema:Organization
    140 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    141 schema:name Biological Sciences
    142 rdf:type schema:DefinedTerm
    143 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    144 schema:name Genetics
    145 rdf:type schema:DefinedTerm
    146 sg:grant.4322241 http://pending.schema.org/fundedItem sg:pub.10.1007/s11120-021-00871-x
    147 rdf:type schema:MonetaryGrant
    148 sg:journal.1022986 schema:issn 0166-8595
    149 1573-5079
    150 schema:name Photosynthesis Research
    151 schema:publisher Springer Nature
    152 rdf:type schema:Periodical
    153 sg:person.01042026314.63 schema:affiliation grid-institutes:grid.215654.1
    154 schema:familyName Baker
    155 schema:givenName Patricia L.
    156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042026314.63
    157 rdf:type schema:Person
    158 sg:person.01102443555.03 schema:affiliation grid-institutes:grid.47100.32
    159 schema:familyName Gisriel
    160 schema:givenName Christopher J.
    161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102443555.03
    162 rdf:type schema:Person
    163 sg:person.01272503314.34 schema:affiliation grid-institutes:grid.215654.1
    164 schema:familyName Redding
    165 schema:givenName Kevin E.
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01272503314.34
    167 rdf:type schema:Person
    168 sg:person.0630653007.39 schema:affiliation grid-institutes:grid.417574.4
    169 schema:familyName Orf
    170 schema:givenName Gregory S.
    171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0630653007.39
    172 rdf:type schema:Person
    173 sg:pub.10.1007/978-1-4020-4256-0_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044688748
    174 https://doi.org/10.1007/978-1-4020-4256-0_13
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1007/978-1-4939-7366-8_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092443730
    177 https://doi.org/10.1007/978-1-4939-7366-8_3
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1007/bf00393378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021995899
    180 https://doi.org/10.1007/bf00393378
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1007/s11120-009-9516-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006689152
    183 https://doi.org/10.1007/s11120-009-9516-1
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1007/s11120-010-9529-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018996460
    186 https://doi.org/10.1007/s11120-010-9529-9
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1007/s11120-012-9726-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049636598
    189 https://doi.org/10.1007/s11120-012-9726-9
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1007/s11120-013-9871-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047180540
    192 https://doi.org/10.1007/s11120-013-9871-9
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1007/s11120-014-0065-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015767636
    195 https://doi.org/10.1007/s11120-014-0065-x
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1007/s11120-017-0401-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1085601724
    198 https://doi.org/10.1007/s11120-017-0401-z
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1007/s11120-018-0496-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1101509061
    201 https://doi.org/10.1007/s11120-018-0496-x
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1007/s11120-018-0503-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101844443
    204 https://doi.org/10.1007/s11120-018-0503-2
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1007/s11120-018-0556-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105775703
    207 https://doi.org/10.1007/s11120-018-0556-2
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1007/s11120-019-00662-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1120095953
    210 https://doi.org/10.1007/s11120-019-00662-5
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1007/s11120-019-00672-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1121175874
    213 https://doi.org/10.1007/s11120-019-00672-3
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1038/35082000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030227276
    216 https://doi.org/10.1038/35082000
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1038/nmeth.f.202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035202423
    219 https://doi.org/10.1038/nmeth.f.202
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1038/nprot.2006.288 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053697239
    222 https://doi.org/10.1038/nprot.2006.288
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1038/nprot.2006.4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031570865
    225 https://doi.org/10.1038/nprot.2006.4
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1038/s41467-019-13898-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1124047941
    228 https://doi.org/10.1038/s41467-019-13898-5
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1038/s41467-021-23060-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1138064085
    231 https://doi.org/10.1038/s41467-021-23060-9
    232 rdf:type schema:CreativeWork
    233 sg:pub.10.1038/srep25666 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051165506
    234 https://doi.org/10.1038/srep25666
    235 rdf:type schema:CreativeWork
    236 grid-institutes:grid.215654.1 schema:alternateName Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA
    237 schema:name Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA
    238 rdf:type schema:Organization
    239 grid-institutes:grid.417574.4 schema:alternateName Infectious Disease Research, Abbott Diagnostics Division, 60064, Abbott Park, IL, USA
    240 schema:name Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA
    241 Infectious Disease Research, Abbott Diagnostics Division, 60064, Abbott Park, IL, USA
    242 rdf:type schema:Organization
    243 grid-institutes:grid.47100.32 schema:alternateName Department of Chemistry, Yale University, 06520, New Haven, CT, USA
    244 schema:name Center for Bioenergy and Photosynthesis, School of Molecular Sciences, Arizona State University, 85287, Tempe, AZ, USA
    245 Department of Chemistry, Yale University, 06520, New Haven, CT, USA
    246 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...