Challenges of metagenomics and single-cell genomics approaches for exploring cyanobacterial diversity View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-10

AUTHORS

Michelle Davison, Eric Hall, Richard Zare, Devaki Bhaya

ABSTRACT

Cyanobacteria have played a crucial role in the history of early earth and continue to be instrumental in shaping our planet, yet applications of cutting edge technology have not yet been widely used to explore cyanobacterial diversity. To provide adequate background, we briefly review current sequencing technologies and their innovative uses in genomics and metagenomics. Next, we focus on current cell capture technologies and the challenges of using them with cyanobacteria. We illustrate the utility in coupling breakthroughs in DNA amplification with cell capture platforms, with an example of microfluidic isolation and subsequent targeted amplicon sequencing from individual terrestrial thermophilic cyanobacteria. Single cells of thermophilic, unicellular Synechococcus sp. JA-2-3-B'a(2-13) (Syn OS-B') were sorted in a microfluidic device, lysed, and subjected to whole genome amplification by multiple displacement amplification. We amplified regions from specific CRISPR spacer arrays, which are known to be highly diverse, contain semi-palindromic repeats which form secondary structure, and can be difficult to amplify. Cell capture, lysis, and genome amplification on a microfluidic device have been optimized, setting a stage for further investigations of individual cyanobacterial cells isolated directly from natural populations. More... »

PAGES

135-146

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11120-014-0066-9

DOI

http://dx.doi.org/10.1007/s11120-014-0066-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040747483

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25515769


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Clustered Regularly Interspaced Short Palindromic Repeats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyanobacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Variation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lab-On-A-Chip Devices", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metagenomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Single-Cell Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Synechococcus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Plant Biology", 
          "id": "https://www.grid.ac/institutes/grid.418000.d", 
          "name": [
            "Department of Plant Biology, Carnegie Institution of Science, 260 Panama Street, 94305, Stanford, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Davison", 
        "givenName": "Michelle", 
        "id": "sg:person.01033002055.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033002055.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "SRI International", 
          "id": "https://www.grid.ac/institutes/grid.98913.3a", 
          "name": [
            "SRI International, 333 Ravenswood Ave, 94025, Menlo Park, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hall", 
        "givenName": "Eric", 
        "id": "sg:person.01163365447.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163365447.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Stanford University", 
          "id": "https://www.grid.ac/institutes/grid.168010.e", 
          "name": [
            "Department of Chemistry, Stanford University, 333 Campus Drive Mudd Building, Room 121, 94305-4401, Stanford, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zare", 
        "givenName": "Richard", 
        "id": "sg:person.010075246350.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010075246350.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Plant Biology", 
          "id": "https://www.grid.ac/institutes/grid.418000.d", 
          "name": [
            "Department of Plant Biology, Carnegie Institution of Science, 260 Panama Street, 94305, Stanford, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhaya", 
        "givenName": "Devaki", 
        "id": "sg:person.01332313235.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332313235.76"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/1-4020-3546-2_12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001300628", 
          "https://doi.org/10.1007/1-4020-3546-2_12"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq1074", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001911032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.01.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003281084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003346160", 
          "https://doi.org/10.1038/nmeth.2783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/genomea.00030-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003404005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12010-008-8348-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004251739", 
          "https://doi.org/10.1007/s12010-008-8348-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0014-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004408222", 
          "https://doi.org/10.1007/s00253-005-0014-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0014-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004408222", 
          "https://doi.org/10.1007/s00253-005-0014-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2013.44", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004512633", 
          "https://doi.org/10.1038/ismej.2013.44"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2012.89", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006043893", 
          "https://doi.org/10.1038/ismej.2012.89"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2012-13-5-r40", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006112329", 
          "https://doi.org/10.1186/gb-2012-13-5-r40"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fmicb.2013.00246", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006517315"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mib.2011.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006821772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00153-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007366196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0004169", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008413083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1556-4029.2006.00172.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008865208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.0030155", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009285468"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0016626", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009547268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/dnares/3.3.109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009801344"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6941.2011.01113.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010534755"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rstb.2006.1919", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011258373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-13-283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011693892", 
          "https://doi.org/10.1186/1471-2105-13-283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-2920.2008.01827.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012223695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00333-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012650057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.margen.2013.12.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012770552"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.01011-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012790943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-genet-110410-132430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013432136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac0340758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014090174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac0340758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014090174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0009773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014104972"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2013.243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015343058", 
          "https://doi.org/10.1038/ismej.2013.243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.68.8.3878-3885.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015572439"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2011.84", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016554900", 
          "https://doi.org/10.1038/ismej.2011.84"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2010.96", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017935221", 
          "https://doi.org/10.1038/ismej.2010.96"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-micro-092412-155713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018053531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cyto.a.22294", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018762505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00792-012-0499-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018875872", 
          "https://doi.org/10.1007/s00792-012-0499-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2010.12.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019496253"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibtech.2010.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020360936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1305867110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020787672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2012.79", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021240493", 
          "https://doi.org/10.1038/ismej.2012.79"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2007.05.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022368575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1217107110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022815476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-7-216", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023149012", 
          "https://doi.org/10.1186/1471-2164-7-216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2011-12-10-230", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023607198", 
          "https://doi.org/10.1186/gb-2011-12-10-230"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2002-3-2-reviews0003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023733744", 
          "https://doi.org/10.1186/gb-2002-3-2-reviews0003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2007.46", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023746914", 
          "https://doi.org/10.1038/ismej.2007.46"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro1939", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023772380", 
          "https://doi.org/10.1038/nrmicro1939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-7-27", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024020431", 
          "https://doi.org/10.1186/1475-2859-7-27"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0801389105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024087450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-bioeng-070909-105238", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024521988"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2011.03.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024819104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms2748", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025004824", 
          "https://doi.org/10.1038/ncomms2748"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/23.5.729", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026583754"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027127512", 
          "https://doi.org/10.1038/nature08284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027127512", 
          "https://doi.org/10.1038/nature08284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1120312109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027668405"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00248-011-9806-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028080479", 
          "https://doi.org/10.1007/s00248-011-9806-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1111712108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028304005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.06354-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029215287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature13459", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030016698", 
          "https://doi.org/10.1038/nature13459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2012.23", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030144325", 
          "https://doi.org/10.1038/ismej.2012.23"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2012.150", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030415053", 
          "https://doi.org/10.1038/ismej.2012.150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-genet-102209-163607", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030772154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0006864", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031074117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c2an15927a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031261737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2013.121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031497587", 
          "https://doi.org/10.1038/ismej.2013.121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2011.73", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031797589", 
          "https://doi.org/10.1038/ismej.2011.73"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-010-2524-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032011894", 
          "https://doi.org/10.1007/s00253-010-2524-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-010-2524-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032011894", 
          "https://doi.org/10.1007/s00253-010-2524-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2010.189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032499026", 
          "https://doi.org/10.1038/ismej.2010.189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35085554", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033061036", 
          "https://doi.org/10.1038/35085554"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35085554", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033061036", 
          "https://doi.org/10.1038/35085554"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11120-013-9890-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033100340", 
          "https://doi.org/10.1007/s11120-013-9890-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0026161", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034910963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1307701110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035436233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biotechadv.2012.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035717147"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.113.221937", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037501338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fmicb.2013.00323", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038570652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0018565", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038893956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13238-010-0043-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039221576", 
          "https://doi.org/10.1007/s13238-010-0043-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13238-010-0043-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039221576", 
          "https://doi.org/10.1007/s13238-010-0043-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-011-3601-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040537136", 
          "https://doi.org/10.1007/s00253-011-3601-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biophys.36.040306.132646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041418307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1522-2683(200210)23:20<3461::aid-elps3461>3.0.co;2-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042481502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2180-7-108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044368108", 
          "https://doi.org/10.1186/1471-2180-7-108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2014.03.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044439504"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1003987", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044628356"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02032-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045014899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-biochem-072911-172315", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046701792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046723721", 
          "https://doi.org/10.1038/nature00917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00917", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046723721", 
          "https://doi.org/10.1038/nature00917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.182.5.1191-1199.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047157035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/mec.12000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047476652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/1574-6976.12015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047563263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1462-2920.2000.00071.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049041560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1213963109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049160601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0005299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049237903"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.00035-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049259434"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1756-0500-2-48", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049943724", 
          "https://doi.org/10.1186/1756-0500-2-48"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-007-3855-3_6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050065499", 
          "https://doi.org/10.1007/978-94-007-3855-3_6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6941.2010.00835.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050326480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tplants.2007.03.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050342882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1011428107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050855390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ismej.2013.83", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050997910", 
          "https://doi.org/10.1038/ismej.2013.83"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.yexcr.2010.02.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051215291"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ece3.593", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051730389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.01610-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052037206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.288.5463.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053301497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2009.00183.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053444408"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.3259624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057926449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/ast.2011.0724", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059233356"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-148-2-453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060371005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1099128", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062449717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1248575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062469263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.274.5289.998", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062554765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.3547653", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062619951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7542800", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062647490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1258/jrsm.95.2.81", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064537454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077121103", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077158954", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077684611", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080731784", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081576153", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083027355", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-10", 
    "datePublishedReg": "2015-10-01", 
    "description": "Cyanobacteria have played a crucial role in the history of early earth and continue to be instrumental in shaping our planet, yet applications of cutting edge technology have not yet been widely used to explore cyanobacterial diversity. To provide adequate background, we briefly review current sequencing technologies and their innovative uses in genomics and metagenomics. Next, we focus on current cell capture technologies and the challenges of using them with cyanobacteria. We illustrate the utility in coupling breakthroughs in DNA amplification with cell capture platforms, with an example of microfluidic isolation and subsequent targeted amplicon sequencing from individual terrestrial thermophilic cyanobacteria. Single cells of thermophilic, unicellular Synechococcus sp. JA-2-3-B'a(2-13) (Syn OS-B') were sorted in a microfluidic device, lysed, and subjected to whole genome amplification by multiple displacement amplification. We amplified regions from specific CRISPR spacer arrays, which are known to be highly diverse, contain semi-palindromic repeats which form secondary structure, and can be difficult to amplify. Cell capture, lysis, and genome amplification on a microfluidic device have been optimized, setting a stage for further investigations of individual cyanobacterial cells isolated directly from natural populations. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11120-014-0066-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1022986", 
        "issn": [
          "0166-8595", 
          "1573-5079"
        ], 
        "name": "Photosynthesis Research", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "126"
      }
    ], 
    "name": "Challenges of metagenomics and single-cell genomics approaches for exploring cyanobacterial diversity", 
    "pagination": "135-146", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c21aa7ed6bbe149181cb242a730dc1d543eb19fcf8da2657069d1a4089c4a634"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25515769"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "100954728"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11120-014-0066-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1040747483"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11120-014-0066-9", 
      "https://app.dimensions.ai/details/publication/pub.1040747483"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000523.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11120-014-0066-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11120-014-0066-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11120-014-0066-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11120-014-0066-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11120-014-0066-9'


 

This table displays all metadata directly associated to this object as RDF triples.

518 TRIPLES      21 PREDICATES      156 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11120-014-0066-9 schema:about N0af5734437424a78b841a2b03c6c88e8
2 N2c5bb0652bf0417ea53a1f5379c9f278
3 N889c30c2f8fa4993b048b0442004ef34
4 N9fd6c336ae324bd69f2c2bb8940c676f
5 Naac6e327f476463aa30c25da4f1c9522
6 Nada50b4859244771b0b4faa1818db538
7 Naf631ed3e85346d2b6b0521b04237e8b
8 Ncf0f77836ae4468985db8f491aca77cc
9 Nfec8787de4954285b0661d22d8dbeb14
10 anzsrc-for:06
11 anzsrc-for:0604
12 schema:author N6aa2a67714b344deb775817cf1fe9670
13 schema:citation sg:pub.10.1007/1-4020-3546-2_12
14 sg:pub.10.1007/978-94-007-3855-3_6
15 sg:pub.10.1007/s00248-011-9806-y
16 sg:pub.10.1007/s00253-005-0014-x
17 sg:pub.10.1007/s00253-010-2524-4
18 sg:pub.10.1007/s00253-011-3601-z
19 sg:pub.10.1007/s00792-012-0499-z
20 sg:pub.10.1007/s11120-013-9890-6
21 sg:pub.10.1007/s12010-008-8348-3
22 sg:pub.10.1007/s13238-010-0043-9
23 sg:pub.10.1038/35085554
24 sg:pub.10.1038/ismej.2007.46
25 sg:pub.10.1038/ismej.2010.189
26 sg:pub.10.1038/ismej.2010.96
27 sg:pub.10.1038/ismej.2011.73
28 sg:pub.10.1038/ismej.2011.84
29 sg:pub.10.1038/ismej.2012.150
30 sg:pub.10.1038/ismej.2012.23
31 sg:pub.10.1038/ismej.2012.79
32 sg:pub.10.1038/ismej.2012.89
33 sg:pub.10.1038/ismej.2013.121
34 sg:pub.10.1038/ismej.2013.243
35 sg:pub.10.1038/ismej.2013.44
36 sg:pub.10.1038/ismej.2013.83
37 sg:pub.10.1038/nature00917
38 sg:pub.10.1038/nature08284
39 sg:pub.10.1038/nature13459
40 sg:pub.10.1038/ncomms2748
41 sg:pub.10.1038/nmeth.2783
42 sg:pub.10.1038/nrmicro1939
43 sg:pub.10.1186/1471-2105-13-283
44 sg:pub.10.1186/1471-2164-7-216
45 sg:pub.10.1186/1471-2180-7-108
46 sg:pub.10.1186/1475-2859-7-27
47 sg:pub.10.1186/1756-0500-2-48
48 sg:pub.10.1186/gb-2002-3-2-reviews0003
49 sg:pub.10.1186/gb-2011-12-10-230
50 sg:pub.10.1186/gb-2012-13-5-r40
51 https://app.dimensions.ai/details/publication/pub.1077121103
52 https://app.dimensions.ai/details/publication/pub.1077158954
53 https://app.dimensions.ai/details/publication/pub.1077684611
54 https://app.dimensions.ai/details/publication/pub.1080731784
55 https://app.dimensions.ai/details/publication/pub.1081576153
56 https://app.dimensions.ai/details/publication/pub.1083027355
57 https://doi.org/10.1002/1522-2683(200210)23:20<3461::aid-elps3461>3.0.co;2-8
58 https://doi.org/10.1002/cyto.a.22294
59 https://doi.org/10.1002/ece3.593
60 https://doi.org/10.1016/j.ab.2007.05.017
61 https://doi.org/10.1016/j.ab.2010.12.028
62 https://doi.org/10.1016/j.biotechadv.2012.05.001
63 https://doi.org/10.1016/j.cell.2011.01.030
64 https://doi.org/10.1016/j.margen.2013.12.006
65 https://doi.org/10.1016/j.mib.2011.07.024
66 https://doi.org/10.1016/j.molcel.2014.03.011
67 https://doi.org/10.1016/j.tibtech.2010.12.003
68 https://doi.org/10.1016/j.tim.2011.03.001
69 https://doi.org/10.1016/j.tplants.2007.03.011
70 https://doi.org/10.1016/j.yexcr.2010.02.036
71 https://doi.org/10.1021/ac0340758
72 https://doi.org/10.1039/c2an15927a
73 https://doi.org/10.1046/j.1462-2920.2000.00071.x
74 https://doi.org/10.1063/1.3259624
75 https://doi.org/10.1073/pnas.0801389105
76 https://doi.org/10.1073/pnas.1011428107
77 https://doi.org/10.1073/pnas.1111712108
78 https://doi.org/10.1073/pnas.1120312109
79 https://doi.org/10.1073/pnas.1213963109
80 https://doi.org/10.1073/pnas.1217107110
81 https://doi.org/10.1073/pnas.1305867110
82 https://doi.org/10.1073/pnas.1307701110
83 https://doi.org/10.1089/ast.2011.0724
84 https://doi.org/10.1093/dnares/3.3.109
85 https://doi.org/10.1093/nar/23.5.729
86 https://doi.org/10.1093/nar/gkq1074
87 https://doi.org/10.1098/rstb.2006.1919
88 https://doi.org/10.1099/00221287-148-2-453
89 https://doi.org/10.1104/pp.113.221937
90 https://doi.org/10.1111/1574-6976.12015
91 https://doi.org/10.1111/j.1462-2920.2008.01827.x
92 https://doi.org/10.1111/j.1556-4029.2006.00172.x
93 https://doi.org/10.1111/j.1574-6941.2010.00835.x
94 https://doi.org/10.1111/j.1574-6941.2011.01113.x
95 https://doi.org/10.1111/j.1574-6976.2009.00183.x
96 https://doi.org/10.1111/mec.12000
97 https://doi.org/10.1126/science.1099128
98 https://doi.org/10.1126/science.1248575
99 https://doi.org/10.1126/science.274.5289.998
100 https://doi.org/10.1126/science.288.5463.113
101 https://doi.org/10.1126/science.3547653
102 https://doi.org/10.1126/science.7542800
103 https://doi.org/10.1128/aem.00153-13
104 https://doi.org/10.1128/aem.02032-10
105 https://doi.org/10.1128/aem.06354-11
106 https://doi.org/10.1128/aem.68.8.3878-3885.2002
107 https://doi.org/10.1128/genomea.00030-12
108 https://doi.org/10.1128/jb.00333-12
109 https://doi.org/10.1128/jb.01011-08
110 https://doi.org/10.1128/jb.01610-09
111 https://doi.org/10.1128/jb.182.5.1191-1199.2000
112 https://doi.org/10.1128/mmbr.00035-08
113 https://doi.org/10.1146/annurev-biochem-072911-172315
114 https://doi.org/10.1146/annurev-bioeng-070909-105238
115 https://doi.org/10.1146/annurev-genet-102209-163607
116 https://doi.org/10.1146/annurev-genet-110410-132430
117 https://doi.org/10.1146/annurev-micro-092412-155713
118 https://doi.org/10.1146/annurev.biophys.36.040306.132646
119 https://doi.org/10.1258/jrsm.95.2.81
120 https://doi.org/10.1371/journal.pgen.0030155
121 https://doi.org/10.1371/journal.pgen.1003987
122 https://doi.org/10.1371/journal.pone.0004169
123 https://doi.org/10.1371/journal.pone.0005299
124 https://doi.org/10.1371/journal.pone.0006864
125 https://doi.org/10.1371/journal.pone.0009773
126 https://doi.org/10.1371/journal.pone.0016626
127 https://doi.org/10.1371/journal.pone.0018565
128 https://doi.org/10.1371/journal.pone.0026161
129 https://doi.org/10.3389/fmicb.2013.00246
130 https://doi.org/10.3389/fmicb.2013.00323
131 schema:datePublished 2015-10
132 schema:datePublishedReg 2015-10-01
133 schema:description Cyanobacteria have played a crucial role in the history of early earth and continue to be instrumental in shaping our planet, yet applications of cutting edge technology have not yet been widely used to explore cyanobacterial diversity. To provide adequate background, we briefly review current sequencing technologies and their innovative uses in genomics and metagenomics. Next, we focus on current cell capture technologies and the challenges of using them with cyanobacteria. We illustrate the utility in coupling breakthroughs in DNA amplification with cell capture platforms, with an example of microfluidic isolation and subsequent targeted amplicon sequencing from individual terrestrial thermophilic cyanobacteria. Single cells of thermophilic, unicellular Synechococcus sp. JA-2-3-B'a(2-13) (Syn OS-B') were sorted in a microfluidic device, lysed, and subjected to whole genome amplification by multiple displacement amplification. We amplified regions from specific CRISPR spacer arrays, which are known to be highly diverse, contain semi-palindromic repeats which form secondary structure, and can be difficult to amplify. Cell capture, lysis, and genome amplification on a microfluidic device have been optimized, setting a stage for further investigations of individual cyanobacterial cells isolated directly from natural populations.
134 schema:genre research_article
135 schema:inLanguage en
136 schema:isAccessibleForFree false
137 schema:isPartOf N60cf4d2835de4c59b052cef157ea7100
138 N8d881605d22949b9b8cf861d1d2318b1
139 sg:journal.1022986
140 schema:name Challenges of metagenomics and single-cell genomics approaches for exploring cyanobacterial diversity
141 schema:pagination 135-146
142 schema:productId N1990784540d344dca6c643150d60a301
143 N23435f8442a346ceb201d17bf0086254
144 N4ba22173016446989ae9e448e571e03f
145 N56cf298f24ae49a9ad83e7f53880b432
146 N7c09b1ce463e4ec99f5ab1b00b155c34
147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040747483
148 https://doi.org/10.1007/s11120-014-0066-9
149 schema:sdDatePublished 2019-04-10T14:12
150 schema:sdLicense https://scigraph.springernature.com/explorer/license/
151 schema:sdPublisher Nb019f54cb4c94ac4aeb70aebcae8cc95
152 schema:url http://link.springer.com/10.1007%2Fs11120-014-0066-9
153 sgo:license sg:explorer/license/
154 sgo:sdDataset articles
155 rdf:type schema:ScholarlyArticle
156 N0ac5071d24af4d3db1ea8ca1255b9ffe rdf:first sg:person.01332313235.76
157 rdf:rest rdf:nil
158 N0af5734437424a78b841a2b03c6c88e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Sequence Analysis, DNA
160 rdf:type schema:DefinedTerm
161 N1990784540d344dca6c643150d60a301 schema:name doi
162 schema:value 10.1007/s11120-014-0066-9
163 rdf:type schema:PropertyValue
164 N23435f8442a346ceb201d17bf0086254 schema:name nlm_unique_id
165 schema:value 100954728
166 rdf:type schema:PropertyValue
167 N2c5bb0652bf0417ea53a1f5379c9f278 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Genetic Variation
169 rdf:type schema:DefinedTerm
170 N4ba22173016446989ae9e448e571e03f schema:name readcube_id
171 schema:value c21aa7ed6bbe149181cb242a730dc1d543eb19fcf8da2657069d1a4089c4a634
172 rdf:type schema:PropertyValue
173 N56cf298f24ae49a9ad83e7f53880b432 schema:name pubmed_id
174 schema:value 25515769
175 rdf:type schema:PropertyValue
176 N60cf4d2835de4c59b052cef157ea7100 schema:issueNumber 1
177 rdf:type schema:PublicationIssue
178 N6aa2a67714b344deb775817cf1fe9670 rdf:first sg:person.01033002055.25
179 rdf:rest N95b80a9727054fa9b42c61aa755efae1
180 N7c09b1ce463e4ec99f5ab1b00b155c34 schema:name dimensions_id
181 schema:value pub.1040747483
182 rdf:type schema:PropertyValue
183 N889c30c2f8fa4993b048b0442004ef34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Metagenomics
185 rdf:type schema:DefinedTerm
186 N8d881605d22949b9b8cf861d1d2318b1 schema:volumeNumber 126
187 rdf:type schema:PublicationVolume
188 N95b80a9727054fa9b42c61aa755efae1 rdf:first sg:person.01163365447.54
189 rdf:rest Nee1ff6fd00754b97a7297eeb7d277c24
190 N9fd6c336ae324bd69f2c2bb8940c676f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name Lab-On-A-Chip Devices
192 rdf:type schema:DefinedTerm
193 Naac6e327f476463aa30c25da4f1c9522 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Single-Cell Analysis
195 rdf:type schema:DefinedTerm
196 Nada50b4859244771b0b4faa1818db538 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
197 schema:name Clustered Regularly Interspaced Short Palindromic Repeats
198 rdf:type schema:DefinedTerm
199 Naf631ed3e85346d2b6b0521b04237e8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Cyanobacteria
201 rdf:type schema:DefinedTerm
202 Nb019f54cb4c94ac4aeb70aebcae8cc95 schema:name Springer Nature - SN SciGraph project
203 rdf:type schema:Organization
204 Ncf0f77836ae4468985db8f491aca77cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
205 schema:name Synechococcus
206 rdf:type schema:DefinedTerm
207 Nee1ff6fd00754b97a7297eeb7d277c24 rdf:first sg:person.010075246350.09
208 rdf:rest N0ac5071d24af4d3db1ea8ca1255b9ffe
209 Nfec8787de4954285b0661d22d8dbeb14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Genomics
211 rdf:type schema:DefinedTerm
212 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
213 schema:name Biological Sciences
214 rdf:type schema:DefinedTerm
215 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
216 schema:name Genetics
217 rdf:type schema:DefinedTerm
218 sg:journal.1022986 schema:issn 0166-8595
219 1573-5079
220 schema:name Photosynthesis Research
221 rdf:type schema:Periodical
222 sg:person.010075246350.09 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
223 schema:familyName Zare
224 schema:givenName Richard
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010075246350.09
226 rdf:type schema:Person
227 sg:person.01033002055.25 schema:affiliation https://www.grid.ac/institutes/grid.418000.d
228 schema:familyName Davison
229 schema:givenName Michelle
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033002055.25
231 rdf:type schema:Person
232 sg:person.01163365447.54 schema:affiliation https://www.grid.ac/institutes/grid.98913.3a
233 schema:familyName Hall
234 schema:givenName Eric
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163365447.54
236 rdf:type schema:Person
237 sg:person.01332313235.76 schema:affiliation https://www.grid.ac/institutes/grid.418000.d
238 schema:familyName Bhaya
239 schema:givenName Devaki
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332313235.76
241 rdf:type schema:Person
242 sg:pub.10.1007/1-4020-3546-2_12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001300628
243 https://doi.org/10.1007/1-4020-3546-2_12
244 rdf:type schema:CreativeWork
245 sg:pub.10.1007/978-94-007-3855-3_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050065499
246 https://doi.org/10.1007/978-94-007-3855-3_6
247 rdf:type schema:CreativeWork
248 sg:pub.10.1007/s00248-011-9806-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1028080479
249 https://doi.org/10.1007/s00248-011-9806-y
250 rdf:type schema:CreativeWork
251 sg:pub.10.1007/s00253-005-0014-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004408222
252 https://doi.org/10.1007/s00253-005-0014-x
253 rdf:type schema:CreativeWork
254 sg:pub.10.1007/s00253-010-2524-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032011894
255 https://doi.org/10.1007/s00253-010-2524-4
256 rdf:type schema:CreativeWork
257 sg:pub.10.1007/s00253-011-3601-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1040537136
258 https://doi.org/10.1007/s00253-011-3601-z
259 rdf:type schema:CreativeWork
260 sg:pub.10.1007/s00792-012-0499-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1018875872
261 https://doi.org/10.1007/s00792-012-0499-z
262 rdf:type schema:CreativeWork
263 sg:pub.10.1007/s11120-013-9890-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033100340
264 https://doi.org/10.1007/s11120-013-9890-6
265 rdf:type schema:CreativeWork
266 sg:pub.10.1007/s12010-008-8348-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004251739
267 https://doi.org/10.1007/s12010-008-8348-3
268 rdf:type schema:CreativeWork
269 sg:pub.10.1007/s13238-010-0043-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039221576
270 https://doi.org/10.1007/s13238-010-0043-9
271 rdf:type schema:CreativeWork
272 sg:pub.10.1038/35085554 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033061036
273 https://doi.org/10.1038/35085554
274 rdf:type schema:CreativeWork
275 sg:pub.10.1038/ismej.2007.46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023746914
276 https://doi.org/10.1038/ismej.2007.46
277 rdf:type schema:CreativeWork
278 sg:pub.10.1038/ismej.2010.189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032499026
279 https://doi.org/10.1038/ismej.2010.189
280 rdf:type schema:CreativeWork
281 sg:pub.10.1038/ismej.2010.96 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017935221
282 https://doi.org/10.1038/ismej.2010.96
283 rdf:type schema:CreativeWork
284 sg:pub.10.1038/ismej.2011.73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031797589
285 https://doi.org/10.1038/ismej.2011.73
286 rdf:type schema:CreativeWork
287 sg:pub.10.1038/ismej.2011.84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016554900
288 https://doi.org/10.1038/ismej.2011.84
289 rdf:type schema:CreativeWork
290 sg:pub.10.1038/ismej.2012.150 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030415053
291 https://doi.org/10.1038/ismej.2012.150
292 rdf:type schema:CreativeWork
293 sg:pub.10.1038/ismej.2012.23 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030144325
294 https://doi.org/10.1038/ismej.2012.23
295 rdf:type schema:CreativeWork
296 sg:pub.10.1038/ismej.2012.79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021240493
297 https://doi.org/10.1038/ismej.2012.79
298 rdf:type schema:CreativeWork
299 sg:pub.10.1038/ismej.2012.89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006043893
300 https://doi.org/10.1038/ismej.2012.89
301 rdf:type schema:CreativeWork
302 sg:pub.10.1038/ismej.2013.121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031497587
303 https://doi.org/10.1038/ismej.2013.121
304 rdf:type schema:CreativeWork
305 sg:pub.10.1038/ismej.2013.243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015343058
306 https://doi.org/10.1038/ismej.2013.243
307 rdf:type schema:CreativeWork
308 sg:pub.10.1038/ismej.2013.44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004512633
309 https://doi.org/10.1038/ismej.2013.44
310 rdf:type schema:CreativeWork
311 sg:pub.10.1038/ismej.2013.83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050997910
312 https://doi.org/10.1038/ismej.2013.83
313 rdf:type schema:CreativeWork
314 sg:pub.10.1038/nature00917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046723721
315 https://doi.org/10.1038/nature00917
316 rdf:type schema:CreativeWork
317 sg:pub.10.1038/nature08284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027127512
318 https://doi.org/10.1038/nature08284
319 rdf:type schema:CreativeWork
320 sg:pub.10.1038/nature13459 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030016698
321 https://doi.org/10.1038/nature13459
322 rdf:type schema:CreativeWork
323 sg:pub.10.1038/ncomms2748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025004824
324 https://doi.org/10.1038/ncomms2748
325 rdf:type schema:CreativeWork
326 sg:pub.10.1038/nmeth.2783 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003346160
327 https://doi.org/10.1038/nmeth.2783
328 rdf:type schema:CreativeWork
329 sg:pub.10.1038/nrmicro1939 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023772380
330 https://doi.org/10.1038/nrmicro1939
331 rdf:type schema:CreativeWork
332 sg:pub.10.1186/1471-2105-13-283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011693892
333 https://doi.org/10.1186/1471-2105-13-283
334 rdf:type schema:CreativeWork
335 sg:pub.10.1186/1471-2164-7-216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023149012
336 https://doi.org/10.1186/1471-2164-7-216
337 rdf:type schema:CreativeWork
338 sg:pub.10.1186/1471-2180-7-108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044368108
339 https://doi.org/10.1186/1471-2180-7-108
340 rdf:type schema:CreativeWork
341 sg:pub.10.1186/1475-2859-7-27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024020431
342 https://doi.org/10.1186/1475-2859-7-27
343 rdf:type schema:CreativeWork
344 sg:pub.10.1186/1756-0500-2-48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049943724
345 https://doi.org/10.1186/1756-0500-2-48
346 rdf:type schema:CreativeWork
347 sg:pub.10.1186/gb-2002-3-2-reviews0003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023733744
348 https://doi.org/10.1186/gb-2002-3-2-reviews0003
349 rdf:type schema:CreativeWork
350 sg:pub.10.1186/gb-2011-12-10-230 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023607198
351 https://doi.org/10.1186/gb-2011-12-10-230
352 rdf:type schema:CreativeWork
353 sg:pub.10.1186/gb-2012-13-5-r40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006112329
354 https://doi.org/10.1186/gb-2012-13-5-r40
355 rdf:type schema:CreativeWork
356 https://app.dimensions.ai/details/publication/pub.1077121103 schema:CreativeWork
357 https://app.dimensions.ai/details/publication/pub.1077158954 schema:CreativeWork
358 https://app.dimensions.ai/details/publication/pub.1077684611 schema:CreativeWork
359 https://app.dimensions.ai/details/publication/pub.1080731784 schema:CreativeWork
360 https://app.dimensions.ai/details/publication/pub.1081576153 schema:CreativeWork
361 https://app.dimensions.ai/details/publication/pub.1083027355 schema:CreativeWork
362 https://doi.org/10.1002/1522-2683(200210)23:20<3461::aid-elps3461>3.0.co;2-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042481502
363 rdf:type schema:CreativeWork
364 https://doi.org/10.1002/cyto.a.22294 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018762505
365 rdf:type schema:CreativeWork
366 https://doi.org/10.1002/ece3.593 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051730389
367 rdf:type schema:CreativeWork
368 https://doi.org/10.1016/j.ab.2007.05.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022368575
369 rdf:type schema:CreativeWork
370 https://doi.org/10.1016/j.ab.2010.12.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019496253
371 rdf:type schema:CreativeWork
372 https://doi.org/10.1016/j.biotechadv.2012.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035717147
373 rdf:type schema:CreativeWork
374 https://doi.org/10.1016/j.cell.2011.01.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003281084
375 rdf:type schema:CreativeWork
376 https://doi.org/10.1016/j.margen.2013.12.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012770552
377 rdf:type schema:CreativeWork
378 https://doi.org/10.1016/j.mib.2011.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006821772
379 rdf:type schema:CreativeWork
380 https://doi.org/10.1016/j.molcel.2014.03.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044439504
381 rdf:type schema:CreativeWork
382 https://doi.org/10.1016/j.tibtech.2010.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020360936
383 rdf:type schema:CreativeWork
384 https://doi.org/10.1016/j.tim.2011.03.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024819104
385 rdf:type schema:CreativeWork
386 https://doi.org/10.1016/j.tplants.2007.03.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050342882
387 rdf:type schema:CreativeWork
388 https://doi.org/10.1016/j.yexcr.2010.02.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051215291
389 rdf:type schema:CreativeWork
390 https://doi.org/10.1021/ac0340758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014090174
391 rdf:type schema:CreativeWork
392 https://doi.org/10.1039/c2an15927a schema:sameAs https://app.dimensions.ai/details/publication/pub.1031261737
393 rdf:type schema:CreativeWork
394 https://doi.org/10.1046/j.1462-2920.2000.00071.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049041560
395 rdf:type schema:CreativeWork
396 https://doi.org/10.1063/1.3259624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057926449
397 rdf:type schema:CreativeWork
398 https://doi.org/10.1073/pnas.0801389105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024087450
399 rdf:type schema:CreativeWork
400 https://doi.org/10.1073/pnas.1011428107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050855390
401 rdf:type schema:CreativeWork
402 https://doi.org/10.1073/pnas.1111712108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028304005
403 rdf:type schema:CreativeWork
404 https://doi.org/10.1073/pnas.1120312109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027668405
405 rdf:type schema:CreativeWork
406 https://doi.org/10.1073/pnas.1213963109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049160601
407 rdf:type schema:CreativeWork
408 https://doi.org/10.1073/pnas.1217107110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022815476
409 rdf:type schema:CreativeWork
410 https://doi.org/10.1073/pnas.1305867110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020787672
411 rdf:type schema:CreativeWork
412 https://doi.org/10.1073/pnas.1307701110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035436233
413 rdf:type schema:CreativeWork
414 https://doi.org/10.1089/ast.2011.0724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059233356
415 rdf:type schema:CreativeWork
416 https://doi.org/10.1093/dnares/3.3.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009801344
417 rdf:type schema:CreativeWork
418 https://doi.org/10.1093/nar/23.5.729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026583754
419 rdf:type schema:CreativeWork
420 https://doi.org/10.1093/nar/gkq1074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001911032
421 rdf:type schema:CreativeWork
422 https://doi.org/10.1098/rstb.2006.1919 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011258373
423 rdf:type schema:CreativeWork
424 https://doi.org/10.1099/00221287-148-2-453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060371005
425 rdf:type schema:CreativeWork
426 https://doi.org/10.1104/pp.113.221937 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037501338
427 rdf:type schema:CreativeWork
428 https://doi.org/10.1111/1574-6976.12015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047563263
429 rdf:type schema:CreativeWork
430 https://doi.org/10.1111/j.1462-2920.2008.01827.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012223695
431 rdf:type schema:CreativeWork
432 https://doi.org/10.1111/j.1556-4029.2006.00172.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008865208
433 rdf:type schema:CreativeWork
434 https://doi.org/10.1111/j.1574-6941.2010.00835.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1050326480
435 rdf:type schema:CreativeWork
436 https://doi.org/10.1111/j.1574-6941.2011.01113.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010534755
437 rdf:type schema:CreativeWork
438 https://doi.org/10.1111/j.1574-6976.2009.00183.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053444408
439 rdf:type schema:CreativeWork
440 https://doi.org/10.1111/mec.12000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047476652
441 rdf:type schema:CreativeWork
442 https://doi.org/10.1126/science.1099128 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062449717
443 rdf:type schema:CreativeWork
444 https://doi.org/10.1126/science.1248575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062469263
445 rdf:type schema:CreativeWork
446 https://doi.org/10.1126/science.274.5289.998 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062554765
447 rdf:type schema:CreativeWork
448 https://doi.org/10.1126/science.288.5463.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053301497
449 rdf:type schema:CreativeWork
450 https://doi.org/10.1126/science.3547653 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062619951
451 rdf:type schema:CreativeWork
452 https://doi.org/10.1126/science.7542800 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062647490
453 rdf:type schema:CreativeWork
454 https://doi.org/10.1128/aem.00153-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007366196
455 rdf:type schema:CreativeWork
456 https://doi.org/10.1128/aem.02032-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045014899
457 rdf:type schema:CreativeWork
458 https://doi.org/10.1128/aem.06354-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029215287
459 rdf:type schema:CreativeWork
460 https://doi.org/10.1128/aem.68.8.3878-3885.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015572439
461 rdf:type schema:CreativeWork
462 https://doi.org/10.1128/genomea.00030-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003404005
463 rdf:type schema:CreativeWork
464 https://doi.org/10.1128/jb.00333-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012650057
465 rdf:type schema:CreativeWork
466 https://doi.org/10.1128/jb.01011-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012790943
467 rdf:type schema:CreativeWork
468 https://doi.org/10.1128/jb.01610-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052037206
469 rdf:type schema:CreativeWork
470 https://doi.org/10.1128/jb.182.5.1191-1199.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047157035
471 rdf:type schema:CreativeWork
472 https://doi.org/10.1128/mmbr.00035-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049259434
473 rdf:type schema:CreativeWork
474 https://doi.org/10.1146/annurev-biochem-072911-172315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046701792
475 rdf:type schema:CreativeWork
476 https://doi.org/10.1146/annurev-bioeng-070909-105238 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024521988
477 rdf:type schema:CreativeWork
478 https://doi.org/10.1146/annurev-genet-102209-163607 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030772154
479 rdf:type schema:CreativeWork
480 https://doi.org/10.1146/annurev-genet-110410-132430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013432136
481 rdf:type schema:CreativeWork
482 https://doi.org/10.1146/annurev-micro-092412-155713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018053531
483 rdf:type schema:CreativeWork
484 https://doi.org/10.1146/annurev.biophys.36.040306.132646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041418307
485 rdf:type schema:CreativeWork
486 https://doi.org/10.1258/jrsm.95.2.81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064537454
487 rdf:type schema:CreativeWork
488 https://doi.org/10.1371/journal.pgen.0030155 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009285468
489 rdf:type schema:CreativeWork
490 https://doi.org/10.1371/journal.pgen.1003987 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044628356
491 rdf:type schema:CreativeWork
492 https://doi.org/10.1371/journal.pone.0004169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008413083
493 rdf:type schema:CreativeWork
494 https://doi.org/10.1371/journal.pone.0005299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049237903
495 rdf:type schema:CreativeWork
496 https://doi.org/10.1371/journal.pone.0006864 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031074117
497 rdf:type schema:CreativeWork
498 https://doi.org/10.1371/journal.pone.0009773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014104972
499 rdf:type schema:CreativeWork
500 https://doi.org/10.1371/journal.pone.0016626 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009547268
501 rdf:type schema:CreativeWork
502 https://doi.org/10.1371/journal.pone.0018565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038893956
503 rdf:type schema:CreativeWork
504 https://doi.org/10.1371/journal.pone.0026161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034910963
505 rdf:type schema:CreativeWork
506 https://doi.org/10.3389/fmicb.2013.00246 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006517315
507 rdf:type schema:CreativeWork
508 https://doi.org/10.3389/fmicb.2013.00323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038570652
509 rdf:type schema:CreativeWork
510 https://www.grid.ac/institutes/grid.168010.e schema:alternateName Stanford University
511 schema:name Department of Chemistry, Stanford University, 333 Campus Drive Mudd Building, Room 121, 94305-4401, Stanford, CA, USA
512 rdf:type schema:Organization
513 https://www.grid.ac/institutes/grid.418000.d schema:alternateName Department of Plant Biology
514 schema:name Department of Plant Biology, Carnegie Institution of Science, 260 Panama Street, 94305, Stanford, CA, USA
515 rdf:type schema:Organization
516 https://www.grid.ac/institutes/grid.98913.3a schema:alternateName SRI International
517 schema:name SRI International, 333 Ravenswood Ave, 94025, Menlo Park, CA, USA
518 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...