Transgenic Tea Over-expressing Solanum tuberosum Endo-1,3-beta-d-glucanase Gene Conferred Resistance Against Blister Blight Disease View Full Text


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Article Info

DATE

2018-01-13

AUTHORS

H. Ranjit Singh, Pranita Hazarika, Niraj Agarwala, Neelakshi Bhattacharyya, Prasenjit Bhagawati, Bornali Gohain, Tirthankar Bandyopadhyay, Raju Bharalee, Sushmita Gupta, Manab Deka, Sudripta Das

ABSTRACT

Tea (Camellia sinensis [L.] O. Kuntze) plant, one of the most important plantation crops in the world, is infected by a fungus called Exobasidium vexans leading to dreaded blister blight disease. The disease may result in crop losses up to 35% which directly affect the tea industry. Solanum tuberosum endo-1,3-beta-d-glucanase was cloned into tea genome via Agrobacterium-mediated transformation. The transformation event initially gave 32 kanamycin-resistant plantlets, out of which PCR analysis confirmed only 10 plantlets about the integration of transgene in the plant genome. Real-time PCR study detected transgene expression in six transgenic plantlets. Upregulation of endogenous C. sinensis pathogenesis-related (PR) genes like PR3 (chitinase I) gene and PR5 (thaumatin-like protein) gene also occurred in transgenic plantlets. Detached leaf infection assay showed resistance to E. vexans in greenhouse-acclimated transgenic plantlets. An inhibitory activity against E. vexans was noticed on the detached leaves of transgenic plantlets compared to control. Transgenic plantlets showed resistance to inoculated fungal pathogen by the formation of hypersensitivity reaction area unlike the formation of fungal lesion on control plantlet. Thus, it can be inferred that constitutive expression of the potato endo-1,3-beta-d-glucanase gene can be a strategy to produce blister blight-resistant tea. More... »

PAGES

107-122

References to SciGraph publications

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  • 1996-09. Constitutive expression of an inducible β-1,3-glucanase in alfalfa reduces disease severity caused by the oomycete pathogenPhytophthora megasperma f. spmedicaginis, but does not reduce disease severity of chitin-containing fungi in TRANSGENIC RESEARCH
  • 2010-10-24. Transformation of sweet orange [Citrus sinensis (L.) Osbeck] with pthA-nls for acquiring resistance to citrus canker disease in PLANT MOLECULAR BIOLOGY
  • 2012-02-15. Genetic engineering of crop plants for fungal resistance: role of antifungal genes in BIOTECHNOLOGY LETTERS
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