Expression of Withania somnifera Steroidal Glucosyltransferase gene Enhances Withanolide Content in Hairy Roots View Full Text


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Article Info

DATE

2015-10-28

AUTHORS

Vibha Pandey, Rakesh Srivastava, Nehal Akhtar, Jyoti Mishra, Pratibha Mishra, Praveen C. Verma

ABSTRACT

In Withania somnifera, sterol molecules of immense medicinal value are diversified by means of glycosylation. Identifying sterol glycosyltransferases provides an imperative insight of diverse sterol modifications, thereby helping to comprehend the underlying plant mechanisms. In the present study, one of the W. somnifera sterol glycosyltransferase-4 (Ws-Sgtl4) gene was transformed into the W. somnifera leaf explant through Agrobacterium rhizogene. Transformed W. Somnifera Ws-Sgtl4 leaf explants were subjected to hairy root induction and analyzed for biomass accumulation. The analysis of Ws-Sgtl4 gene expression was performed at different time exposures with the application of salicylic acid and methyl jasmonate. The elicitation of W. somnifera hairy root expressing the Ws-Sgtl4 gene was also evaluated for the enhancement if any, in the total withanolide yield as well as the withanolides-A contents. The results suggested that Ws-Sgtl4 gene expression enhanced the production of total withanolide yield and withanolides-A in the hairy root culture of W. somnifera in the response to the elicitors. More... »

PAGES

681-689

References to SciGraph publications

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  • 2009-09-08. Glycyrrhizin production by in vitro cultured Glycyrrhiza glabra elicited by methyl Jasmonate and salicylic acid in RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
  • 2012-06-29. Sterol glycosyltransferases-identification of members of gene family and their role in stress in Withania somnifera in MOLECULAR BIOLOGY REPORTS
  • 2010-09-25. Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation in BIOTECHNOLOGY LETTERS
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  • 2014-12-18. Expression of Rabies Glycoprotein and Ricin Toxin B Chain (RGP–RTB) Fusion Protein in Tomato Hairy Roots: A Step Towards Oral Vaccination for Rabies in MOLECULAR BIOTECHNOLOGY
  • 2012-06-26. Withanolide biosynthesis recruits both mevalonate and DOXP pathways of isoprenogenesis in Ashwagandha Withania somnifera L. (Dunal) in PLANT CELL REPORTS
  • 2009-04-19. Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2013-08-22. Withaferin A: a proteasomal inhibitor promotes healing after injury and exerts anabolic effect on osteoporotic bone in CELL DEATH & DISEASE
  • 2007-01-11. Stimulation of saponin production in Panax ginseng hairy roots by two oligosaccharides from Paris polyphylla var. yunnanensis in BIOTECHNOLOGY LETTERS
  • 2015-04-16. Comparative interactions of withanolides and sterols with two members of sterol glycosyltransferases from Withania somnifera in BMC BIOINFORMATICS
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