Simultaneous Overexpression of Citrate Synthase and Phosphoenolpyruvate Carboxylase in Leaves Augments Citrate Exclusion and Al Resistance in Transgenic Tobacco View Full Text


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Article Info

DATE

2012-01-25

AUTHORS

Qifeng Wang, Qiong Yi, Qingquan Hu, Yue Zhao, Hongjuan Nian, Kunzhi Li, Yongxiong Yu, Katsura Izui, Limei Chen

ABSTRACT

Phosphoenolpyruvate carboxylase (PEPC) and citrate synthase (CS) are two key enzymes in organic acid synthesis metabolism. In the present study, a cytoplasmic form of CS from tobacco and a mutant (with reduced sensitivity to organic acid inhibition) PEPC from Synechococcus vulcanus were overexpressed simultaneously using a light-inducible promoter in tobacco leaves. The analysis for enzyme activity showed that CS and PEPC enzyme activities were increased by 235% to 257% and 218% to 236% in the selected cs and pepc (double-gene) overexpression lines, respectively, compared with those in the wild-type plants (WT). The measurement for the relative root elongation rate of the tobacco plants exposed to 30 μM aluminum (Al) indicated that Al tolerance in the double-gene overexpression lines was stronger than that of the transgenic cs or pepc lines and WT plants. The 13C-NMR analysis with NaH13CO3 showed that overexpression of CS and PEPC in the transgenic tobacco successfully constructed a new citrate synthesis pathway. Under the conditions with Al stress, the amount of citrate secreted from the double-transgenic tobacco roots was the largest among the tested plants. When grown on sandy soil supplied with a nutritional solution containing 500 μM Al, the growth of the double-transgenic tobacco was better than that of the transgenic cs or pepc tobacco and WT, and their root biomass was the highest among the tested plants. These results demonstrated that construction of a new citrate synthesis pathway by simultaneous overexpression of CS and PEPC in the cytoplasm of transgenic plant leaves could enhance Al resistance in plants. More... »

PAGES

992-1005

References to SciGraph publications

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  • 2010-05-25. Overexpression of malate dehydrogenase in transgenic tobacco leaves: enhanced malate synthesis and augmented Al-resistance in ACTA PHYSIOLOGIAE PLANTARUM
  • 2010-09-01. Ovexpression of a Vacuolar H+-ATPase c Subunit Gene Mediates Physiological Changes Leading to Enhanced Salt Tolerance in Transgenic Tobacco in PLANT MOLECULAR BIOLOGY REPORTER
  • 2008-02-28. Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa in PLANT CELL REPORTS
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  • 1991. Identifying plants adaptable to low pH conditions in PLANT-SOIL INTERACTIONS AT LOW PH
  • 2011-10-08. Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics in PLANT MOLECULAR BIOLOGY REPORTER
  • 2010-11-04. Overexpression of Petunia SOC1-like Gene FBP21 in Tobacco Promotes Flowering Without Decreasing Flower or Fruit Quantity in PLANT MOLECULAR BIOLOGY REPORTER
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