Ontology type: schema:ScholarlyArticle
2011-03-30
AUTHORSXiaoxia Jin, Zhiwei Qin, Tao Wu, Xiuyan Zhou
ABSTRACTEthylene is one of the most important hormones for flowering of cucumber (Cucumis sativus L.). The molecular interaction between cucumber and ethylene was investigated by construction and characterization of a suppression subtractive hybridization cDNA library from stem apex of ethrel-treated cucumber. To screen differentially expressed genes, dot blotting was used with cDNA probes prepared from mRNAs isolated from control and ethrel-treated samples. Acquired positive clones were subjected to sequence and homology search analysis. In total, 103 expressed sequence tags (ESTs) were acquired after screening; among these, 73 showed significant sequence similarity to known genes, 26 had unknown functions, and four clones did not match any known sequences. Gene ontology (GO) analysis revealed that ESTs with known functions were involved in photosynthesis, metabolism, protein synthesis, cellular communication and transport, signal transduction, and transcriptional regulation. The expression levels and overall profiles in female and male buds of 11 ESTs were confirmed by reverse transcription polymerase chain reaction (RT–PCR) and three of them were detected by real-time quantitative PCR (qRT–PCR) in the whole plant. One of these was an F-box protein, EBF1 (EIN3 BINDING F-BOX1), which might play a positive role in female expression of cucumber. More... »
PAGES875-884
http://scigraph.springernature.com/pub.10.1007/s11105-011-0304-7
DOIhttp://dx.doi.org/10.1007/s11105-011-0304-7
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1047314642
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China",
"id": "http://www.grid.ac/institutes/grid.412243.2",
"name": [
"Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China"
],
"type": "Organization"
},
"familyName": "Jin",
"givenName": "Xiaoxia",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China",
"id": "http://www.grid.ac/institutes/grid.412243.2",
"name": [
"Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China"
],
"type": "Organization"
},
"familyName": "Qin",
"givenName": "Zhiwei",
"id": "sg:person.01316036127.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316036127.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China",
"id": "http://www.grid.ac/institutes/grid.412243.2",
"name": [
"Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China"
],
"type": "Organization"
},
"familyName": "Wu",
"givenName": "Tao",
"id": "sg:person.0620073165.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0620073165.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China",
"id": "http://www.grid.ac/institutes/grid.412243.2",
"name": [
"Department of Horticulture, Northeast Agricultural University, 150030, Harbin, China"
],
"type": "Organization"
},
"familyName": "Zhou",
"givenName": "Xiuyan",
"id": "sg:person.016427000027.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016427000027.08"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s11105-010-0206-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009879346",
"https://doi.org/10.1007/s11105-010-0206-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrg1601",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016764611",
"https://doi.org/10.1038/nrg1601"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-008-0023-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044759639",
"https://doi.org/10.1007/s11105-008-0023-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature05960",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027567406",
"https://doi.org/10.1038/nature05960"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature07289",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000012565",
"https://doi.org/10.1038/nature07289"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-010-0222-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007666274",
"https://doi.org/10.1007/s11105-010-0222-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00497-003-0183-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045559602",
"https://doi.org/10.1007/s00497-003-0183-7"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02174037",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025957428",
"https://doi.org/10.1007/bf02174037"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1005896711321",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026021832",
"https://doi.org/10.1023/a:1005896711321"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-009-0105-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006608327",
"https://doi.org/10.1007/s11105-009-0105-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-008-0081-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014502355",
"https://doi.org/10.1007/s11105-008-0081-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1005967106993",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038994535",
"https://doi.org/10.1023/a:1005967106993"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00425-004-1342-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000076248",
"https://doi.org/10.1007/s00425-004-1342-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00425-002-0953-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1075267222",
"https://doi.org/10.1007/s00425-002-0953-8"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature08498",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052355281",
"https://doi.org/10.1038/nature08498"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00122-004-1793-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013436662",
"https://doi.org/10.1007/s00122-004-1793-7"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature06132",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027953315",
"https://doi.org/10.1038/nature06132"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-010-0249-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052137589",
"https://doi.org/10.1007/s11105-010-0249-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-010-0229-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036562076",
"https://doi.org/10.1007/s11105-010-0229-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11105-009-0103-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000059796",
"https://doi.org/10.1007/s11105-009-0103-6"
],
"type": "CreativeWork"
}
],
"datePublished": "2011-03-30",
"datePublishedReg": "2011-03-30",
"description": "Ethylene is one of the most important hormones for flowering of cucumber (Cucumis sativus L.). The molecular interaction between cucumber and ethylene was investigated by construction and characterization of a suppression subtractive hybridization cDNA library from stem apex of ethrel-treated cucumber. To screen differentially expressed genes, dot blotting was used with cDNA probes prepared from mRNAs isolated from control and ethrel-treated samples. Acquired positive clones were subjected to sequence and homology search analysis. In total, 103 expressed sequence tags (ESTs) were acquired after screening; among these, 73 showed significant sequence similarity to known genes, 26 had unknown functions, and four clones did not match any known sequences. Gene ontology (GO) analysis revealed that ESTs with known functions were involved in photosynthesis, metabolism, protein synthesis, cellular communication and transport, signal transduction, and transcriptional regulation. The expression levels and overall profiles in female and male buds of 11 ESTs were confirmed by reverse transcription polymerase chain reaction (RT\u2013PCR) and three of them were detected by real-time quantitative PCR (qRT\u2013PCR) in the whole plant. One of these was an F-box protein, EBF1 (EIN3 BINDING F-BOX1), which might play a positive role in female expression of cucumber.",
"genre": "article",
"id": "sg:pub.10.1007/s11105-011-0304-7",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1124107",
"issn": [
"0735-9640",
"1556-469X"
],
"name": "Plant Molecular Biology Reporter",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "29"
}
],
"keywords": [
"suppression subtractive hybridization cDNA library",
"ethylene-responsive genes",
"F-box proteins",
"significant sequence similarity",
"suppression subtractive hybridization",
"Gene ontology analysis",
"homology search analysis",
"transcriptional regulation",
"sequence tags",
"sequence similarity",
"ontology analysis",
"subtractive hybridization",
"signal transduction",
"real-time quantitative PCR",
"shoot apex",
"cDNA library",
"male buds",
"known sequences",
"unknown function",
"stem apex",
"whole plant",
"positive clones",
"protein synthesis",
"cDNA probe",
"female expression",
"genes",
"cucumber",
"search analysis",
"expression levels",
"quantitative PCR",
"molecular interactions",
"ESTs",
"dot blotting",
"cellular communication",
"important hormone",
"reverse transcription-polymerase chain reaction",
"clones",
"transcription-polymerase chain reaction",
"Ethrel",
"polymerase chain reaction",
"sequence",
"positive role",
"photosynthesis",
"EBF1",
"transduction",
"chain reaction",
"plants",
"protein",
"buds",
"mRNA",
"hybridization",
"regulation",
"blotting",
"expression",
"metabolism",
"apex",
"ethylene",
"PCR",
"tags",
"function",
"screen",
"similarity",
"identification",
"overall profile",
"library",
"role",
"probe",
"characterization",
"interaction",
"hormone",
"transport",
"analysis",
"synthesis",
"levels",
"profile",
"control",
"total",
"samples",
"reaction",
"construction",
"communication"
],
"name": "Identification of Ethylene-Responsive Genes in Ethrel-Treated Shoot Apices of Cucumber by Suppression Subtractive Hybridization",
"pagination": "875-884",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1047314642"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11105-011-0304-7"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11105-011-0304-7",
"https://app.dimensions.ai/details/publication/pub.1047314642"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:58",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_543.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s11105-011-0304-7"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11105-011-0304-7'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11105-011-0304-7'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11105-011-0304-7'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11105-011-0304-7'
This table displays all metadata directly associated to this object as RDF triples.
238 TRIPLES
21 PREDICATES
125 URIs
97 LITERALS
6 BLANK NODES