The Arabidopsis E1 subunit of the 2-oxoglutarate dehydrogenase complex modulates plant growth and seed production View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-07-08

AUTHORS

Jorge A. Condori-Apfata, Willian Batista-Silva, David Barbosa Medeiros, Jonas Rafael Vargas, Luiz Mário Lopes Valente, Elmien Heyneke, Jorge Luis Pérez-Diaz, Alisdair R. Fernie, Wagner L. Araújo, Adriano Nunes-Nesi

ABSTRACT

Key messageIsoforms of 2-OGDH E1 subunit are not functionally redundant in plant growth and development of A. thaliana.AbstractThe tricarboxylic acid cycle enzyme 2-oxoglutarate dehydrogenase (2-OGDH) converts 2-oxoglutarate (2-OG) to succinyl-CoA concomitant with the reduction of NAD+. 2-OGDH has an essential role in plant metabolism, being both a limiting step during mitochondrial respiration as well as a key player in carbon–nitrogen interactions. In Arabidopsis thaliana two genes encode for E1 subunit of 2-OGDH but the physiological roles of each isoform remain unknown. Thus, in the present study we isolated Arabidopsis T-DNA insertion knockout mutant lines for each of the genes encoding the E1 subunit of 2-OGDH enzyme. All mutant plants exhibited substantial reduction in both respiration and CO2 assimilation rates. Furthermore, mutant lines exhibited reduced levels of chlorophylls and nitrate, increased levels of sucrose, malate and fumarate and minor changes in total protein and starch levels in leaves. Despite the similar metabolic phenotypes for the two E1 isoforms the reduction in the expression of each gene culminated in different responses in terms of plant growth and seed production indicating distinct roles for each isoform. Collectively, our results demonstrated the importance of the E1 subunit of 2-OGDH in both autotrophic and heterotrophic tissues and suggest that the two E1 isoforms are not functionally redundant in terms of plant growth in A. thaliana. More... »

PAGES

183-202

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11103-019-00900-3

DOI

http://dx.doi.org/10.1007/s11103-019-00900-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1117831838

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31286324


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46 dehydrogenase
47 development
48 different responses
49 distinct roles
50 enzyme
51 essential role
52 expression
53 genes
54 growth
55 heterotrophic tissues
56 importance
57 interaction
58 isoforms
59 key players
60 knockout mutant lines
61 leaves
62 levels
63 levels of sucrose
64 lines
65 metabolic phenotype
66 metabolism
67 minor changes
68 mitochondrial respiration
69 modulate plant growth
70 mutant lines
71 mutant plants
72 nitrate
73 phenotype
74 physiological role
75 plant growth
76 plant metabolism
77 plants
78 players
79 present study
80 production
81 protein
82 rate
83 reduced levels
84 reduction
85 respiration
86 response
87 results
88 role
89 seed production
90 similar metabolic phenotypes
91 starch levels
92 step
93 study
94 substantial reduction
95 subunits
96 sucrose
97 terms
98 thaliana
99 tissue
100 total protein
101 tricarboxylic acid cycle enzymes
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