Transposon insertion in a cinnamyl alcohol dehydrogenase gene is responsible for a brown midrib1 mutation in maize View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-07-31

AUTHORS

Wei Chen, Nathan VanOpdorp, Dennis Fitzl, Jagdish Tewari, Peter Friedemann, Tom Greene, Steve Thompson, Siva Kumpatla, Peizhong Zheng

ABSTRACT

Maize brownmidrib1 (bm1) mutant plants have reduced lignin content and offer significant advantages when used in silage and biofuel applications. Cinnamyl alcohol dehydrogenase (CAD) catalyzes the conversion of hydroxycinnamyl aldehydes to monolignols, a key step in lignin biosynthesis. Maize CAD2 has been implicated as the underlying gene for bm1phenotypes since bm1 plants have reduced CAD activity and lower CAD2 transcript level. Here, we describe a Dow AgroSciences maize bm1 mutant (bm1-das1) that contains a 3,444-bp transposon insertion in the first intron of CAD2 gene. As a result of chimeric RNA splicing, cad2 mRNA from bm1-das1 contains a 409-bp insert between its 1st and 2nd exons. This insertion creates a premature stop codon and is predicted to result in a truncated protein of 48 amino acids (AA), compared to 367 AA for the wild type (WT) CAD2. We have also sequenced cad2 from the reference allele bm1-ref in 515D bm1 stock and showed that it contains a two-nucleotide (AC) insertion in the 3rd exon, which is predicted to result in a truncated protein of 147 AA. The levels of cad2 mRNA in the midribs of bm1-das1 and bm1-ref are reduced by 91 and 86 % respectively, leading to reductions in total lignin contents by 24 and 30 %. Taken together, our data show that mutations in maize CAD2 are responsible for maize bm1 phenotypes. Based on specific changes in bm1-das1 and bm1-ref, high throughput TaqMan and KBioscience’s allele specific PCR assays capable of differentiating mutant and WT alleles have been developed to accelerate bm1 molecular breeding. More... »

PAGES

289-297

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11103-012-9948-4

DOI

http://dx.doi.org/10.1007/s11103-012-9948-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041744020

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22847075


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alcohol Oxidoreductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alleles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Codon, Nonsense", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Frameshift Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Enzymologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lignin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Insertional", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Leaves", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Stems", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zea mays", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Wei", 
        "id": "sg:person.0663177655.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663177655.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "VanOpdorp", 
        "givenName": "Nathan", 
        "id": "sg:person.0731313055.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731313055.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fitzl", 
        "givenName": "Dennis", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tewari", 
        "givenName": "Jagdish", 
        "id": "sg:person.0653111673.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653111673.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Friedemann", 
        "givenName": "Peter", 
        "id": "sg:person.01113654655.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113654655.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pioneer Hi-Bred International, Inc., 7300 NW 62nd Av., 50131, Johnston, IA, USA", 
          "id": "http://www.grid.ac/institutes/grid.292487.2", 
          "name": [
            "Pioneer Hi-Bred International, Inc., 7300 NW 62nd Av., 50131, Johnston, IA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Greene", 
        "givenName": "Tom", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thompson", 
        "givenName": "Steve", 
        "id": "sg:person.01230103255.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230103255.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kumpatla", 
        "givenName": "Siva", 
        "id": "sg:person.01032130304.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032130304.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA", 
          "id": "http://www.grid.ac/institutes/grid.418574.b", 
          "name": [
            "Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zheng", 
        "givenName": "Peizhong", 
        "id": "sg:person.01344331655.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344331655.02"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00425-004-1385-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013337025", 
          "https://doi.org/10.1007/s00425-004-1385-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07992", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028276115", 
          "https://doi.org/10.1038/nature07992"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00202964", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007788229", 
          "https://doi.org/10.1007/bf00202964"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.hdy.6800078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002081344", 
          "https://doi.org/10.1038/sj.hdy.6800078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11103-006-9049-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016335131", 
          "https://doi.org/10.1007/s11103-006-9049-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-006-0468-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010040974", 
          "https://doi.org/10.1007/s00425-006-0468-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-0-387-77863-1_38", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031841575", 
          "https://doi.org/10.1007/978-0-387-77863-1_38"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12155-009-9041-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031210108", 
          "https://doi.org/10.1007/s12155-009-9041-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1009606422975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005545770", 
          "https://doi.org/10.1023/a:1009606422975"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-07-31", 
    "datePublishedReg": "2012-07-31", 
    "description": "Maize brownmidrib1 (bm1) mutant plants have reduced lignin content and offer significant advantages when used in silage and biofuel applications. Cinnamyl alcohol dehydrogenase (CAD) catalyzes the conversion of hydroxycinnamyl aldehydes to monolignols, a key step in lignin biosynthesis. Maize CAD2 has been implicated as the underlying gene for bm1phenotypes since bm1 plants have reduced CAD activity and lower CAD2 transcript level. Here, we describe a Dow AgroSciences maize bm1 mutant (bm1-das1) that contains a 3,444-bp transposon insertion in the first intron of CAD2 gene. As a result of chimeric RNA splicing, cad2 mRNA from bm1-das1 contains a 409-bp insert between its 1st and 2nd exons. This insertion creates a premature stop codon and is predicted to result in a truncated protein of 48 amino acids (AA), compared to 367 AA for the wild type (WT) CAD2. We have also sequenced cad2 from the reference allele bm1-ref in 515D bm1 stock and showed that it contains a two-nucleotide (AC) insertion in the 3rd exon, which is predicted to result in a truncated protein of 147 AA. The levels of cad2 mRNA in the midribs of bm1-das1 and bm1-ref are reduced by 91 and 86\u00a0% respectively, leading to reductions in total lignin contents by 24 and 30\u00a0%. Taken together, our data show that mutations in maize CAD2 are responsible for maize bm1 phenotypes. Based on specific changes in bm1-das1 and bm1-ref, high throughput TaqMan and KBioscience\u2019s allele specific PCR assays capable of differentiating mutant and WT alleles have been developed to accelerate bm1 molecular breeding.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s11103-012-9948-4", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "80"
      }
    ], 
    "keywords": [
      "cinnamyl alcohol dehydrogenase", 
      "allele-specific PCR assays", 
      "amino acids", 
      "transposon insertion", 
      "cinnamyl alcohol dehydrogenase gene", 
      "alcohol dehydrogenase gene", 
      "two-nucleotide insertion", 
      "mutant plants", 
      "molecular breeding", 
      "premature stop codon", 
      "lignin biosynthesis", 
      "CAD2 gene", 
      "RNA splicing", 
      "underlying genes", 
      "dehydrogenase gene", 
      "total lignin content", 
      "first intron", 
      "transcript levels", 
      "specific PCR assays", 
      "stop codon", 
      "CAD activity", 
      "WT allele", 
      "lignin content", 
      "CAD2", 
      "genes", 
      "high-throughput TaqMan", 
      "biofuel applications", 
      "alcohol dehydrogenase", 
      "exons", 
      "reference allele", 
      "plants", 
      "protein", 
      "PCR assays", 
      "mutations", 
      "alleles", 
      "mRNA", 
      "specific changes", 
      "introns", 
      "splicing", 
      "mutants", 
      "breeding", 
      "biosynthesis", 
      "codon", 
      "monolignols", 
      "insertion", 
      "midrib", 
      "key step", 
      "maize", 
      "phenotype", 
      "dehydrogenase", 
      "hydroxycinnamyl", 
      "assays", 
      "inserts", 
      "TaqMan", 
      "acid", 
      "levels", 
      "activity", 
      "content", 
      "stocks", 
      "changes", 
      "step", 
      "conversion", 
      "silage", 
      "data", 
      "results", 
      "reduction", 
      "significant advantages", 
      "advantages", 
      "applications"
    ], 
    "name": "Transposon insertion in a cinnamyl alcohol dehydrogenase gene is responsible for a brown midrib1 mutation in maize", 
    "pagination": "289-297", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1041744020"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11103-012-9948-4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22847075"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11103-012-9948-4", 
      "https://app.dimensions.ai/details/publication/pub.1041744020"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-06-01T22:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_582.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s11103-012-9948-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11103-012-9948-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11103-012-9948-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11103-012-9948-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11103-012-9948-4'


 

This table displays all metadata directly associated to this object as RDF triples.

304 TRIPLES      22 PREDICATES      124 URIs      107 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11103-012-9948-4 schema:about N0b71086c4dfa416b8aaf46a97312f39c
2 N158e9d398d0a46ba9865673c57273643
3 N2500428dc2c546fca92108357b2edcd0
4 N319daa280c8140d7a5d3bf0bedfd2d2b
5 N3d72e5122369488e962025342886f8d8
6 N5577520be311415e8a2951cbc0524e4e
7 N5f1fde5552034d43afa1457cf92b9023
8 N6d64470823cb4aff898937d45e7ec354
9 N6dea1861e3c44d4992afc63a57dfbfa1
10 N73f6cac6e9604312a2e743c3e5d44be0
11 N833d81815d8e4154855fe0c825bec171
12 N8b1ed064242f43bb94cfc1dfaaf87851
13 N9112162c6e014937b60d0f8aa06b0f4f
14 Nae634f1869b54b83810205427b0554d2
15 Nbda5b10b69c9457aaea4433719d06bf2
16 Nbe55506d412d4e7d89df91a833f2a229
17 Nc7e84e02eccc4388a841689c8a69bbcf
18 Nd46696ef86854231b1857cf5f512358c
19 Ndb90f21195414a5db1f730183df4a773
20 Neae46a148e8a4e2babada700c562d57a
21 anzsrc-for:06
22 anzsrc-for:0604
23 schema:author N15b05b178efd45b38afcfbfebe1d71f3
24 schema:citation sg:pub.10.1007/978-0-387-77863-1_38
25 sg:pub.10.1007/bf00202964
26 sg:pub.10.1007/s00425-004-1385-4
27 sg:pub.10.1007/s00425-006-0468-9
28 sg:pub.10.1007/s11103-006-9049-3
29 sg:pub.10.1007/s12155-009-9041-2
30 sg:pub.10.1023/a:1009606422975
31 sg:pub.10.1038/nature07992
32 sg:pub.10.1038/sj.hdy.6800078
33 schema:datePublished 2012-07-31
34 schema:datePublishedReg 2012-07-31
35 schema:description Maize brownmidrib1 (bm1) mutant plants have reduced lignin content and offer significant advantages when used in silage and biofuel applications. Cinnamyl alcohol dehydrogenase (CAD) catalyzes the conversion of hydroxycinnamyl aldehydes to monolignols, a key step in lignin biosynthesis. Maize CAD2 has been implicated as the underlying gene for bm1phenotypes since bm1 plants have reduced CAD activity and lower CAD2 transcript level. Here, we describe a Dow AgroSciences maize bm1 mutant (bm1-das1) that contains a 3,444-bp transposon insertion in the first intron of CAD2 gene. As a result of chimeric RNA splicing, cad2 mRNA from bm1-das1 contains a 409-bp insert between its 1st and 2nd exons. This insertion creates a premature stop codon and is predicted to result in a truncated protein of 48 amino acids (AA), compared to 367 AA for the wild type (WT) CAD2. We have also sequenced cad2 from the reference allele bm1-ref in 515D bm1 stock and showed that it contains a two-nucleotide (AC) insertion in the 3rd exon, which is predicted to result in a truncated protein of 147 AA. The levels of cad2 mRNA in the midribs of bm1-das1 and bm1-ref are reduced by 91 and 86 % respectively, leading to reductions in total lignin contents by 24 and 30 %. Taken together, our data show that mutations in maize CAD2 are responsible for maize bm1 phenotypes. Based on specific changes in bm1-das1 and bm1-ref, high throughput TaqMan and KBioscience’s allele specific PCR assays capable of differentiating mutant and WT alleles have been developed to accelerate bm1 molecular breeding.
36 schema:genre article
37 schema:inLanguage en
38 schema:isAccessibleForFree false
39 schema:isPartOf Nc48c4caa6dfb4148bebb85c1fdcd420d
40 Ne54a366038d54f85a00eb8e1b8b57f92
41 sg:journal.1101246
42 schema:keywords CAD activity
43 CAD2
44 CAD2 gene
45 PCR assays
46 RNA splicing
47 TaqMan
48 WT allele
49 acid
50 activity
51 advantages
52 alcohol dehydrogenase
53 alcohol dehydrogenase gene
54 allele-specific PCR assays
55 alleles
56 amino acids
57 applications
58 assays
59 biofuel applications
60 biosynthesis
61 breeding
62 changes
63 cinnamyl alcohol dehydrogenase
64 cinnamyl alcohol dehydrogenase gene
65 codon
66 content
67 conversion
68 data
69 dehydrogenase
70 dehydrogenase gene
71 exons
72 first intron
73 genes
74 high-throughput TaqMan
75 hydroxycinnamyl
76 insertion
77 inserts
78 introns
79 key step
80 levels
81 lignin biosynthesis
82 lignin content
83 mRNA
84 maize
85 midrib
86 molecular breeding
87 monolignols
88 mutant plants
89 mutants
90 mutations
91 phenotype
92 plants
93 premature stop codon
94 protein
95 reduction
96 reference allele
97 results
98 significant advantages
99 silage
100 specific PCR assays
101 specific changes
102 splicing
103 step
104 stocks
105 stop codon
106 total lignin content
107 transcript levels
108 transposon insertion
109 two-nucleotide insertion
110 underlying genes
111 schema:name Transposon insertion in a cinnamyl alcohol dehydrogenase gene is responsible for a brown midrib1 mutation in maize
112 schema:pagination 289-297
113 schema:productId N00ee304d5dd54c05838887262a15d71b
114 N06a86775435f42b89ec5ea1b3e0dfe12
115 N14677834e15d4579a98424c00b8391b0
116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041744020
117 https://doi.org/10.1007/s11103-012-9948-4
118 schema:sdDatePublished 2022-06-01T22:10
119 schema:sdLicense https://scigraph.springernature.com/explorer/license/
120 schema:sdPublisher N8d3aaefdadc0486e86dfe1b6272f7dc9
121 schema:url https://doi.org/10.1007/s11103-012-9948-4
122 sgo:license sg:explorer/license/
123 sgo:sdDataset articles
124 rdf:type schema:ScholarlyArticle
125 N00ee304d5dd54c05838887262a15d71b schema:name dimensions_id
126 schema:value pub.1041744020
127 rdf:type schema:PropertyValue
128 N06a86775435f42b89ec5ea1b3e0dfe12 schema:name pubmed_id
129 schema:value 22847075
130 rdf:type schema:PropertyValue
131 N06f080e08634471faff94758fbd8f4e5 rdf:first sg:person.0653111673.57
132 rdf:rest N9314e97b8b9d4b9e9b6b8938ca15dc60
133 N09ac6ca7ec324e48b87d9e0419593d69 rdf:first sg:person.01344331655.02
134 rdf:rest rdf:nil
135 N0b71086c4dfa416b8aaf46a97312f39c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Molecular Sequence Data
137 rdf:type schema:DefinedTerm
138 N14677834e15d4579a98424c00b8391b0 schema:name doi
139 schema:value 10.1007/s11103-012-9948-4
140 rdf:type schema:PropertyValue
141 N158e9d398d0a46ba9865673c57273643 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Phenotype
143 rdf:type schema:DefinedTerm
144 N15b05b178efd45b38afcfbfebe1d71f3 rdf:first sg:person.0663177655.69
145 rdf:rest Nddefbfb958d943599fe8d7e3b8f4c18e
146 N2500428dc2c546fca92108357b2edcd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Lignin
148 rdf:type schema:DefinedTerm
149 N293de29a3fde4002866d0b40896a4212 schema:affiliation grid-institutes:grid.418574.b
150 schema:familyName Fitzl
151 schema:givenName Dennis
152 rdf:type schema:Person
153 N29646beae20f4203b60c19fe120f5b6b schema:affiliation grid-institutes:grid.292487.2
154 schema:familyName Greene
155 schema:givenName Tom
156 rdf:type schema:Person
157 N319daa280c8140d7a5d3bf0bedfd2d2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Plant Proteins
159 rdf:type schema:DefinedTerm
160 N3d72e5122369488e962025342886f8d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Cloning, Molecular
162 rdf:type schema:DefinedTerm
163 N42cd84b6c6b04f6f8b569f1f31740254 rdf:first sg:person.01230103255.51
164 rdf:rest Na880e8895b4d4ff0aba6f7deac842136
165 N5577520be311415e8a2951cbc0524e4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name RNA, Messenger
167 rdf:type schema:DefinedTerm
168 N5f1fde5552034d43afa1457cf92b9023 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Frameshift Mutation
170 rdf:type schema:DefinedTerm
171 N6d64470823cb4aff898937d45e7ec354 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name RNA, Plant
173 rdf:type schema:DefinedTerm
174 N6dea1861e3c44d4992afc63a57dfbfa1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Alleles
176 rdf:type schema:DefinedTerm
177 N73f6cac6e9604312a2e743c3e5d44be0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Zea mays
179 rdf:type schema:DefinedTerm
180 N833d81815d8e4154855fe0c825bec171 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Gene Expression Regulation, Enzymologic
182 rdf:type schema:DefinedTerm
183 N8b1ed064242f43bb94cfc1dfaaf87851 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Mutation
185 rdf:type schema:DefinedTerm
186 N8d3aaefdadc0486e86dfe1b6272f7dc9 schema:name Springer Nature - SN SciGraph project
187 rdf:type schema:Organization
188 N9112162c6e014937b60d0f8aa06b0f4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name DNA, Plant
190 rdf:type schema:DefinedTerm
191 N9314e97b8b9d4b9e9b6b8938ca15dc60 rdf:first sg:person.01113654655.20
192 rdf:rest Nd781082635444f47ad900e9ed54f63a8
193 Na880e8895b4d4ff0aba6f7deac842136 rdf:first sg:person.01032130304.58
194 rdf:rest N09ac6ca7ec324e48b87d9e0419593d69
195 Nae634f1869b54b83810205427b0554d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
196 schema:name Alcohol Oxidoreductases
197 rdf:type schema:DefinedTerm
198 Nbda5b10b69c9457aaea4433719d06bf2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Codon, Nonsense
200 rdf:type schema:DefinedTerm
201 Nbe55506d412d4e7d89df91a833f2a229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
202 schema:name Gene Expression Regulation, Plant
203 rdf:type schema:DefinedTerm
204 Nc48c4caa6dfb4148bebb85c1fdcd420d schema:volumeNumber 80
205 rdf:type schema:PublicationVolume
206 Nc7e84e02eccc4388a841689c8a69bbcf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name Mutagenesis, Insertional
208 rdf:type schema:DefinedTerm
209 Nceab5f1cb84942719b835c12a5c58177 rdf:first N293de29a3fde4002866d0b40896a4212
210 rdf:rest N06f080e08634471faff94758fbd8f4e5
211 Nd46696ef86854231b1857cf5f512358c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Base Sequence
213 rdf:type schema:DefinedTerm
214 Nd781082635444f47ad900e9ed54f63a8 rdf:first N29646beae20f4203b60c19fe120f5b6b
215 rdf:rest N42cd84b6c6b04f6f8b569f1f31740254
216 Ndb90f21195414a5db1f730183df4a773 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
217 schema:name Plant Stems
218 rdf:type schema:DefinedTerm
219 Nddefbfb958d943599fe8d7e3b8f4c18e rdf:first sg:person.0731313055.63
220 rdf:rest Nceab5f1cb84942719b835c12a5c58177
221 Ne54a366038d54f85a00eb8e1b8b57f92 schema:issueNumber 3
222 rdf:type schema:PublicationIssue
223 Neae46a148e8a4e2babada700c562d57a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
224 schema:name Plant Leaves
225 rdf:type schema:DefinedTerm
226 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
227 schema:name Biological Sciences
228 rdf:type schema:DefinedTerm
229 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
230 schema:name Genetics
231 rdf:type schema:DefinedTerm
232 sg:journal.1101246 schema:issn 0167-4412
233 1573-5028
234 schema:name Plant Molecular Biology
235 schema:publisher Springer Nature
236 rdf:type schema:Periodical
237 sg:person.01032130304.58 schema:affiliation grid-institutes:grid.418574.b
238 schema:familyName Kumpatla
239 schema:givenName Siva
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01032130304.58
241 rdf:type schema:Person
242 sg:person.01113654655.20 schema:affiliation grid-institutes:grid.418574.b
243 schema:familyName Friedemann
244 schema:givenName Peter
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113654655.20
246 rdf:type schema:Person
247 sg:person.01230103255.51 schema:affiliation grid-institutes:grid.418574.b
248 schema:familyName Thompson
249 schema:givenName Steve
250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230103255.51
251 rdf:type schema:Person
252 sg:person.01344331655.02 schema:affiliation grid-institutes:grid.418574.b
253 schema:familyName Zheng
254 schema:givenName Peizhong
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01344331655.02
256 rdf:type schema:Person
257 sg:person.0653111673.57 schema:affiliation grid-institutes:grid.418574.b
258 schema:familyName Tewari
259 schema:givenName Jagdish
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653111673.57
261 rdf:type schema:Person
262 sg:person.0663177655.69 schema:affiliation grid-institutes:grid.418574.b
263 schema:familyName Chen
264 schema:givenName Wei
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663177655.69
266 rdf:type schema:Person
267 sg:person.0731313055.63 schema:affiliation grid-institutes:grid.418574.b
268 schema:familyName VanOpdorp
269 schema:givenName Nathan
270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0731313055.63
271 rdf:type schema:Person
272 sg:pub.10.1007/978-0-387-77863-1_38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031841575
273 https://doi.org/10.1007/978-0-387-77863-1_38
274 rdf:type schema:CreativeWork
275 sg:pub.10.1007/bf00202964 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007788229
276 https://doi.org/10.1007/bf00202964
277 rdf:type schema:CreativeWork
278 sg:pub.10.1007/s00425-004-1385-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013337025
279 https://doi.org/10.1007/s00425-004-1385-4
280 rdf:type schema:CreativeWork
281 sg:pub.10.1007/s00425-006-0468-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010040974
282 https://doi.org/10.1007/s00425-006-0468-9
283 rdf:type schema:CreativeWork
284 sg:pub.10.1007/s11103-006-9049-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016335131
285 https://doi.org/10.1007/s11103-006-9049-3
286 rdf:type schema:CreativeWork
287 sg:pub.10.1007/s12155-009-9041-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031210108
288 https://doi.org/10.1007/s12155-009-9041-2
289 rdf:type schema:CreativeWork
290 sg:pub.10.1023/a:1009606422975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005545770
291 https://doi.org/10.1023/a:1009606422975
292 rdf:type schema:CreativeWork
293 sg:pub.10.1038/nature07992 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028276115
294 https://doi.org/10.1038/nature07992
295 rdf:type schema:CreativeWork
296 sg:pub.10.1038/sj.hdy.6800078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002081344
297 https://doi.org/10.1038/sj.hdy.6800078
298 rdf:type schema:CreativeWork
299 grid-institutes:grid.292487.2 schema:alternateName Pioneer Hi-Bred International, Inc., 7300 NW 62nd Av., 50131, Johnston, IA, USA
300 schema:name Pioneer Hi-Bred International, Inc., 7300 NW 62nd Av., 50131, Johnston, IA, USA
301 rdf:type schema:Organization
302 grid-institutes:grid.418574.b schema:alternateName Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA
303 schema:name Dow AgroSciences LLC, 9330 Zionsville Road, 46268, Indianapolis, IN, USA
304 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...