Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-02-21

AUTHORS

Mariel C. Gerrard Wheeler, Cintia L. Arias, Marcos A. Tronconi, Verónica G. Maurino, Carlos S. Andreo, María F. Drincovich

ABSTRACT

The Arabidopsis thaliana genome contains four NADP-malic enzymes genes (NADP-ME1-4). NADP-ME4 is localized to plastids whereas the other isoforms are cytosolic. NADP-ME2 and 4 are constitutively expressed, while NADP-ME1 is restricted to secondary roots and NADP-ME3 to trichomes and pollen. Although the four isoforms share remarkably high degree of identity (75–90%), recombinant NADP-ME1 through 4 show distinct kinetic properties, both in the forward (malate oxidative decarboxylation) and reverse (pyruvate reductive carboxylation) reactions. The four isoforms behave differently in terms of reversibility, with NADP-ME2 presenting the highest reverse catalytic efficiency. When analyzing the activity of each isoform in the presence of metabolic effectors, NADP-ME2 was the most highly regulated isoform, especially in its activation by certain effectors. Several metabolites modulate both the forward and reverse reactions, exhibiting dual effects in some cases. Therefore, pyruvate reductive carboxylation may be relevant in vivo, especially in some cellular compartments and conditions. In order to identify residues or segments of the NADP-ME primary structure that could be involved in the differences among the isoforms, NADP-ME2 mutants and deletions were analysed. The results obtained show that Arg115 is involved in fumarate activation, while the amino-terminal part is critical for aspartate and CoA activation, as well as for the reverse reaction. As a whole, these studies show that minimal changes in the primary structure are responsible for the different kinetic behaviour of each AtNADP-ME isoform. In this way, the co-expression of some isoforms in the same cellular compartment would not imply redundancy but represents specificity of function. More... »

PAGES

231-242

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11103-008-9313-9

DOI

http://dx.doi.org/10.1007/s11103-008-9313-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005250253

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18288573


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arabidopsis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arabidopsis Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Primers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fumarates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Isoenzymes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malate Dehydrogenase (NADP+)", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Deletion", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina", 
          "id": "http://www.grid.ac/institutes/grid.506344.0", 
          "name": [
            "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gerrard Wheeler", 
        "givenName": "Mariel C.", 
        "id": "sg:person.01150375457.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150375457.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina", 
          "id": "http://www.grid.ac/institutes/grid.506344.0", 
          "name": [
            "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arias", 
        "givenName": "Cintia L.", 
        "id": "sg:person.01276451741.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276451741.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina", 
          "id": "http://www.grid.ac/institutes/grid.506344.0", 
          "name": [
            "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tronconi", 
        "givenName": "Marcos A.", 
        "id": "sg:person.01311160011.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311160011.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Botanisches Institut, Universit\u00e4t zu K\u00f6ln, Gyrhofstr. 15, 50931, Cologne, Germany", 
          "id": "http://www.grid.ac/institutes/grid.6190.e", 
          "name": [
            "Botanisches Institut, Universit\u00e4t zu K\u00f6ln, Gyrhofstr. 15, 50931, Cologne, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maurino", 
        "givenName": "Ver\u00f3nica G.", 
        "id": "sg:person.01226066653.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226066653.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina", 
          "id": "http://www.grid.ac/institutes/grid.506344.0", 
          "name": [
            "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Andreo", 
        "givenName": "Carlos S.", 
        "id": "sg:person.0671355734.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671355734.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina", 
          "id": "http://www.grid.ac/institutes/grid.506344.0", 
          "name": [
            "Centro de Estudios Fotosint\u00e9ticos y Bioqu\u00edmicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Drincovich", 
        "givenName": "Mar\u00eda F.", 
        "id": "sg:person.0657424310.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657424310.33"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00029456", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037889370", 
          "https://doi.org/10.1007/bf00029456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11103-004-0472-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025393897", 
          "https://doi.org/10.1007/s11103-004-0472-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-005-0044-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020878851", 
          "https://doi.org/10.1007/s00425-005-0044-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004250000345", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005919681", 
          "https://doi.org/10.1007/s004250000345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/73378", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015464026", 
          "https://doi.org/10.1038/73378"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2008-02-21", 
    "datePublishedReg": "2008-02-21", 
    "description": "The Arabidopsis thaliana genome contains four NADP-malic enzymes genes (NADP-ME1-4). NADP-ME4 is localized to plastids whereas the other isoforms are cytosolic. NADP-ME2 and 4 are constitutively expressed, while NADP-ME1 is restricted to secondary roots and NADP-ME3 to trichomes and pollen. Although the four isoforms share remarkably high degree of identity (75\u201390%), recombinant NADP-ME1 through 4 show distinct kinetic properties, both in the forward (malate oxidative decarboxylation) and reverse (pyruvate reductive carboxylation) reactions. The four isoforms behave differently in terms of reversibility, with NADP-ME2 presenting the highest reverse catalytic efficiency. When analyzing the activity of each isoform in the presence of metabolic effectors, NADP-ME2 was the most highly regulated isoform, especially in its activation by certain effectors. Several metabolites modulate both the forward and reverse reactions, exhibiting dual effects in some cases. Therefore, pyruvate reductive carboxylation may be relevant in\u00a0vivo, especially in some cellular compartments and conditions. In order to identify residues or segments of the NADP-ME primary structure that could be involved in the differences among the isoforms, NADP-ME2 mutants and deletions were analysed. The results obtained show that Arg115 is involved in fumarate activation, while the amino-terminal part is critical for aspartate and CoA activation, as well as for the reverse reaction. As a whole, these studies show that minimal changes in the primary structure are responsible for the different kinetic behaviour of each AtNADP-ME isoform. In this way, the co-expression of some isoforms in the same cellular compartment would not imply redundancy but represents specificity of function.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s11103-008-9313-9", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "67"
      }
    ], 
    "keywords": [
      "NADP-ME2", 
      "NADP-ME1", 
      "cellular compartments", 
      "primary structure", 
      "Arabidopsis thaliana genome", 
      "amino-terminal part", 
      "same cellular compartment", 
      "specificity of function", 
      "distinct kinetic properties", 
      "thaliana genome", 
      "NADP-ME3", 
      "NADP-ME4", 
      "isoforms share", 
      "fumarate activation", 
      "CoA activation", 
      "secondary roots", 
      "reductive carboxylation", 
      "certain effectors", 
      "enzyme isoforms", 
      "enzyme genes", 
      "metabolic effectors", 
      "isoforms", 
      "effectors", 
      "catalytic efficiency", 
      "kinetic properties", 
      "reverse reaction", 
      "activation", 
      "distinct properties", 
      "high degree", 
      "Arabidopsis", 
      "compartments", 
      "plastids", 
      "genome", 
      "trichomes", 
      "mutants", 
      "genes", 
      "Arg115", 
      "deletion", 
      "residues", 
      "carboxylation", 
      "pollen", 
      "dual effect", 
      "terms of reversibility", 
      "identity", 
      "roots", 
      "vivo", 
      "aspartate", 
      "metabolites", 
      "specificity", 
      "different kinetic behavior", 
      "structure", 
      "activity", 
      "function", 
      "kinetic behavior", 
      "redundancy", 
      "segments", 
      "presence", 
      "minimal changes", 
      "changes", 
      "reaction", 
      "conditions", 
      "degree", 
      "part", 
      "effect", 
      "study", 
      "differences", 
      "results", 
      "properties", 
      "whole", 
      "efficiency", 
      "reversibility", 
      "order", 
      "share", 
      "behavior", 
      "way", 
      "terms", 
      "cases", 
      "NADP-malic enzymes genes", 
      "recombinant NADP-ME1", 
      "show distinct kinetic properties", 
      "highest reverse catalytic efficiency", 
      "reverse catalytic efficiency", 
      "NADP-ME primary structure", 
      "NADP-ME2 mutants", 
      "AtNADP-ME isoform", 
      "NADP-malic enzyme isoforms"
    ], 
    "name": "Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties", 
    "pagination": "231-242", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005250253"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11103-008-9313-9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18288573"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11103-008-9313-9", 
      "https://app.dimensions.ai/details/publication/pub.1005250253"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_477.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s11103-008-9313-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11103-008-9313-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11103-008-9313-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11103-008-9313-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11103-008-9313-9'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      22 PREDICATES      130 URIs      116 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11103-008-9313-9 schema:about N0e2f25b714224a729a02662418d36008
2 N29d3b561f80f4d6ba3706ff18f448720
3 N2e49b7f9101a4175b80e4d89f2c8bcda
4 N45da172e49ca493b850a48ae56262b1d
5 N58186868158a488c98bea00e6bc25501
6 N6d53b7610a2c4ccc9adb9beb7eec158b
7 N9aeb203126354d8cb5304b91f3ba616a
8 Na33c78ca86aa41d29f5cf341cb5c49c9
9 Nbc2a040347714083b7d7c0716ab50e6b
10 Nd39308b964af4534944229b583786370
11 Nd3bf472197a9471691d27487c543d7b8
12 Ndb4dbd8aab824cdc9258a81cec17a2ca
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N215749bf18fe40288c3a20a7c22dc561
16 schema:citation sg:pub.10.1007/bf00029456
17 sg:pub.10.1007/s00425-005-0044-8
18 sg:pub.10.1007/s004250000345
19 sg:pub.10.1007/s11103-004-0472-z
20 sg:pub.10.1038/227680a0
21 sg:pub.10.1038/73378
22 schema:datePublished 2008-02-21
23 schema:datePublishedReg 2008-02-21
24 schema:description The Arabidopsis thaliana genome contains four NADP-malic enzymes genes (NADP-ME1-4). NADP-ME4 is localized to plastids whereas the other isoforms are cytosolic. NADP-ME2 and 4 are constitutively expressed, while NADP-ME1 is restricted to secondary roots and NADP-ME3 to trichomes and pollen. Although the four isoforms share remarkably high degree of identity (75–90%), recombinant NADP-ME1 through 4 show distinct kinetic properties, both in the forward (malate oxidative decarboxylation) and reverse (pyruvate reductive carboxylation) reactions. The four isoforms behave differently in terms of reversibility, with NADP-ME2 presenting the highest reverse catalytic efficiency. When analyzing the activity of each isoform in the presence of metabolic effectors, NADP-ME2 was the most highly regulated isoform, especially in its activation by certain effectors. Several metabolites modulate both the forward and reverse reactions, exhibiting dual effects in some cases. Therefore, pyruvate reductive carboxylation may be relevant in vivo, especially in some cellular compartments and conditions. In order to identify residues or segments of the NADP-ME primary structure that could be involved in the differences among the isoforms, NADP-ME2 mutants and deletions were analysed. The results obtained show that Arg115 is involved in fumarate activation, while the amino-terminal part is critical for aspartate and CoA activation, as well as for the reverse reaction. As a whole, these studies show that minimal changes in the primary structure are responsible for the different kinetic behaviour of each AtNADP-ME isoform. In this way, the co-expression of some isoforms in the same cellular compartment would not imply redundancy but represents specificity of function.
25 schema:genre article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N0419c8c4409e4bbba478ce4bb69ab52c
29 Nef222c820c2b49cfabf1a79642574e9a
30 sg:journal.1101246
31 schema:keywords Arabidopsis
32 Arabidopsis thaliana genome
33 Arg115
34 AtNADP-ME isoform
35 CoA activation
36 NADP-ME primary structure
37 NADP-ME1
38 NADP-ME2
39 NADP-ME2 mutants
40 NADP-ME3
41 NADP-ME4
42 NADP-malic enzyme isoforms
43 NADP-malic enzymes genes
44 activation
45 activity
46 amino-terminal part
47 aspartate
48 behavior
49 carboxylation
50 cases
51 catalytic efficiency
52 cellular compartments
53 certain effectors
54 changes
55 compartments
56 conditions
57 degree
58 deletion
59 differences
60 different kinetic behavior
61 distinct kinetic properties
62 distinct properties
63 dual effect
64 effect
65 effectors
66 efficiency
67 enzyme genes
68 enzyme isoforms
69 fumarate activation
70 function
71 genes
72 genome
73 high degree
74 highest reverse catalytic efficiency
75 identity
76 isoforms
77 isoforms share
78 kinetic behavior
79 kinetic properties
80 metabolic effectors
81 metabolites
82 minimal changes
83 mutants
84 order
85 part
86 plastids
87 pollen
88 presence
89 primary structure
90 properties
91 reaction
92 recombinant NADP-ME1
93 reductive carboxylation
94 redundancy
95 residues
96 results
97 reverse catalytic efficiency
98 reverse reaction
99 reversibility
100 roots
101 same cellular compartment
102 secondary roots
103 segments
104 share
105 show distinct kinetic properties
106 specificity
107 specificity of function
108 structure
109 study
110 terms
111 terms of reversibility
112 thaliana genome
113 trichomes
114 vivo
115 way
116 whole
117 schema:name Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties
118 schema:pagination 231-242
119 schema:productId N06834346146341efbf5a0e69a5ab7504
120 Na915c90af6bc40b0bcdf7dfbcc0364d6
121 Ndca85a4e27104188887cc0796e084882
122 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005250253
123 https://doi.org/10.1007/s11103-008-9313-9
124 schema:sdDatePublished 2022-01-01T18:18
125 schema:sdLicense https://scigraph.springernature.com/explorer/license/
126 schema:sdPublisher N2c4b7ee82a594a8497ded0563d8250d0
127 schema:url https://doi.org/10.1007/s11103-008-9313-9
128 sgo:license sg:explorer/license/
129 sgo:sdDataset articles
130 rdf:type schema:ScholarlyArticle
131 N0419c8c4409e4bbba478ce4bb69ab52c schema:issueNumber 3
132 rdf:type schema:PublicationIssue
133 N06834346146341efbf5a0e69a5ab7504 schema:name dimensions_id
134 schema:value pub.1005250253
135 rdf:type schema:PropertyValue
136 N0e2f25b714224a729a02662418d36008 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Binding Sites
138 rdf:type schema:DefinedTerm
139 N215749bf18fe40288c3a20a7c22dc561 rdf:first sg:person.01150375457.27
140 rdf:rest N297a7498863a4d4ab0ba487159f5a264
141 N297a7498863a4d4ab0ba487159f5a264 rdf:first sg:person.01276451741.05
142 rdf:rest N6e02d500f23640f0bd1b6fb880aedbdb
143 N29d3b561f80f4d6ba3706ff18f448720 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Molecular Sequence Data
145 rdf:type schema:DefinedTerm
146 N2c4b7ee82a594a8497ded0563d8250d0 schema:name Springer Nature - SN SciGraph project
147 rdf:type schema:Organization
148 N2e49b7f9101a4175b80e4d89f2c8bcda schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name DNA Primers
150 rdf:type schema:DefinedTerm
151 N45da172e49ca493b850a48ae56262b1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Mutagenesis
153 rdf:type schema:DefinedTerm
154 N4df13368edbd4c81b25172eeb9382da1 rdf:first sg:person.01226066653.72
155 rdf:rest N7a2758c987574ca9a25ee2b4b9b8fd31
156 N58186868158a488c98bea00e6bc25501 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Sequence Deletion
158 rdf:type schema:DefinedTerm
159 N6d53b7610a2c4ccc9adb9beb7eec158b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Amino Acid Sequence
161 rdf:type schema:DefinedTerm
162 N6e02d500f23640f0bd1b6fb880aedbdb rdf:first sg:person.01311160011.61
163 rdf:rest N4df13368edbd4c81b25172eeb9382da1
164 N7a2758c987574ca9a25ee2b4b9b8fd31 rdf:first sg:person.0671355734.95
165 rdf:rest Ne5bcd9b0d1974ecea4e87fdeea8395b8
166 N9aeb203126354d8cb5304b91f3ba616a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Kinetics
168 rdf:type schema:DefinedTerm
169 Na33c78ca86aa41d29f5cf341cb5c49c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Arabidopsis Proteins
171 rdf:type schema:DefinedTerm
172 Na915c90af6bc40b0bcdf7dfbcc0364d6 schema:name doi
173 schema:value 10.1007/s11103-008-9313-9
174 rdf:type schema:PropertyValue
175 Nbc2a040347714083b7d7c0716ab50e6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Isoenzymes
177 rdf:type schema:DefinedTerm
178 Nd39308b964af4534944229b583786370 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Arabidopsis
180 rdf:type schema:DefinedTerm
181 Nd3bf472197a9471691d27487c543d7b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Malate Dehydrogenase (NADP+)
183 rdf:type schema:DefinedTerm
184 Ndb4dbd8aab824cdc9258a81cec17a2ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Fumarates
186 rdf:type schema:DefinedTerm
187 Ndca85a4e27104188887cc0796e084882 schema:name pubmed_id
188 schema:value 18288573
189 rdf:type schema:PropertyValue
190 Ne5bcd9b0d1974ecea4e87fdeea8395b8 rdf:first sg:person.0657424310.33
191 rdf:rest rdf:nil
192 Nef222c820c2b49cfabf1a79642574e9a schema:volumeNumber 67
193 rdf:type schema:PublicationVolume
194 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
195 schema:name Biological Sciences
196 rdf:type schema:DefinedTerm
197 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
198 schema:name Biochemistry and Cell Biology
199 rdf:type schema:DefinedTerm
200 sg:journal.1101246 schema:issn 0167-4412
201 1573-5028
202 schema:name Plant Molecular Biology
203 schema:publisher Springer Nature
204 rdf:type schema:Periodical
205 sg:person.01150375457.27 schema:affiliation grid-institutes:grid.506344.0
206 schema:familyName Gerrard Wheeler
207 schema:givenName Mariel C.
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150375457.27
209 rdf:type schema:Person
210 sg:person.01226066653.72 schema:affiliation grid-institutes:grid.6190.e
211 schema:familyName Maurino
212 schema:givenName Verónica G.
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01226066653.72
214 rdf:type schema:Person
215 sg:person.01276451741.05 schema:affiliation grid-institutes:grid.506344.0
216 schema:familyName Arias
217 schema:givenName Cintia L.
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276451741.05
219 rdf:type schema:Person
220 sg:person.01311160011.61 schema:affiliation grid-institutes:grid.506344.0
221 schema:familyName Tronconi
222 schema:givenName Marcos A.
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311160011.61
224 rdf:type schema:Person
225 sg:person.0657424310.33 schema:affiliation grid-institutes:grid.506344.0
226 schema:familyName Drincovich
227 schema:givenName María F.
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657424310.33
229 rdf:type schema:Person
230 sg:person.0671355734.95 schema:affiliation grid-institutes:grid.506344.0
231 schema:familyName Andreo
232 schema:givenName Carlos S.
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671355734.95
234 rdf:type schema:Person
235 sg:pub.10.1007/bf00029456 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037889370
236 https://doi.org/10.1007/bf00029456
237 rdf:type schema:CreativeWork
238 sg:pub.10.1007/s00425-005-0044-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020878851
239 https://doi.org/10.1007/s00425-005-0044-8
240 rdf:type schema:CreativeWork
241 sg:pub.10.1007/s004250000345 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005919681
242 https://doi.org/10.1007/s004250000345
243 rdf:type schema:CreativeWork
244 sg:pub.10.1007/s11103-004-0472-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1025393897
245 https://doi.org/10.1007/s11103-004-0472-z
246 rdf:type schema:CreativeWork
247 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
248 https://doi.org/10.1038/227680a0
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/73378 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015464026
251 https://doi.org/10.1038/73378
252 rdf:type schema:CreativeWork
253 grid-institutes:grid.506344.0 schema:alternateName Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina
254 schema:name Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina
255 rdf:type schema:Organization
256 grid-institutes:grid.6190.e schema:alternateName Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, 50931, Cologne, Germany
257 schema:name Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, 50931, Cologne, Germany
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...