Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-07

AUTHORS

Donald Livingstone, Vadim Beilinson, Marina Kalyaeva, Monica A. Schmidt, Eliot M. Herman, Niels C. Nielsen

ABSTRACT

A mutant Bowman-Birk gene was created that encoded an inactive high-sulfur product. It was used to transform soybean line Asgrow 3237. Transformants bearing the mutant gene were identified by GUS expression, PCR analysis, and Southern analysis. The amount of steady state mRNA from the mutant gene in the transformed plants showed that the gene was highly expressed, but the amount of message from the unmodified Bowman-Birk gene did not change detectably. Proteins synthesized at the direction of the mutant Bowman-Birk gene accumulated in seeds of the transformed plants, and there was a marked decrease in the ability of extracts prepared from these seeds to inhibit trypsin and chymotrypsin despite the presence of Kunitz trypsin inhibitor. The more prevalent mRNA from the mutant gene was considered to out-compete message from the native genes to decrease the amount of active Bowman-Birk inhibitor. More... »

PAGES

397-408

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11103-007-9163-x

DOI

http://dx.doi.org/10.1007/s11103-007-9163-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003017702

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17429741


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Seeds", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Alignment", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Soybeans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trypsin Inhibitor, Bowman-Birk Soybean", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trypsin Inhibitor, Kunitz Soybean", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trypsin Inhibitors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Purdue University", 
          "id": "https://www.grid.ac/institutes/grid.169077.e", 
          "name": [
            "Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Livingstone", 
        "givenName": "Donald", 
        "id": "sg:person.01370756605.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370756605.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Purdue University", 
          "id": "https://www.grid.ac/institutes/grid.169077.e", 
          "name": [
            "Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA", 
            "Athenix Corporation, 2202 Ellis Rd, 27703, Durham, NC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beilinson", 
        "givenName": "Vadim", 
        "id": "sg:person.0670015020.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670015020.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Simplot (United States)", 
          "id": "https://www.grid.ac/institutes/grid.451298.3", 
          "name": [
            "Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA", 
            "J.R. Simplot Company, 999 Main St., Suite 1300, 83702, Boise, ID, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kalyaeva", 
        "givenName": "Marina", 
        "id": "sg:person.0610577775.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610577775.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Donald Danforth Plant Science Center", 
          "id": "https://www.grid.ac/institutes/grid.34424.35", 
          "name": [
            "Agricultural Research Service, United States Department of Agriculture, St. Louis, USA", 
            "Donald Danforth Plant Science Center, 975 Warson Rd, 63132, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schmidt", 
        "givenName": "Monica A.", 
        "id": "sg:person.01077053611.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077053611.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Donald Danforth Plant Science Center", 
          "id": "https://www.grid.ac/institutes/grid.34424.35", 
          "name": [
            "Agricultural Research Service, United States Department of Agriculture, St. Louis, USA", 
            "Donald Danforth Plant Science Center, 975 Warson Rd, 63132, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Herman", 
        "givenName": "Eliot M.", 
        "id": "sg:person.01356203765.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01356203765.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "North Carolina State University", 
          "id": "https://www.grid.ac/institutes/grid.40803.3f", 
          "name": [
            "Agricultural Research Service, United States Department of Agriculture, West Lafayette, USA", 
            "Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA", 
            "Department of Crop Science, North Carolina State University, 27695, Raleigh, NC, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nielsen", 
        "givenName": "Niels C.", 
        "id": "sg:person.01030217545.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030217545.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1104/pp.74.3.576", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001599135"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2795(77)90392-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009535433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2795(77)90392-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009535433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.80.7.1897", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011917250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/0007166031000085463", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018361618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1985.tb02155.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019116996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1985.tb02155.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019116996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.94.16.8393", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019659434"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.000653", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020010270"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.81.3.802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023050999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.1.11.1079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030259842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/313810a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035513087", 
          "https://doi.org/10.1038/313810a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02663049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035873596", 
          "https://doi.org/10.1007/bf02663049"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.80.2.561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037221519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4757-0022-0_1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039643970", 
          "https://doi.org/10.1007/978-1-4757-0022-0_1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.104.056614", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039980918"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.8.5.873", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041586080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bi.49.070180.003113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042085921"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0888-915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043462937", 
          "https://doi.org/10.1038/nbt0888-915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pmic.200500503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043915885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/pmic.200500503", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043915885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jsfa.2740330707", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045472089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00260489", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047369882", 
          "https://doi.org/10.1007/bf00260489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00260489", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047369882", 
          "https://doi.org/10.1007/bf00260489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1071/pp9780641", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050813146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(92)90157-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050848607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(92)90157-k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050848607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0021859699007443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054075133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00651a021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055184075"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00837a032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055190247"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf00078a028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055895442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf971086r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055930076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf971086r", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055930076"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.236.4806.1299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062534239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2135/cropsci1986.0011183x002600030058x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069020883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2135/cropsci2001.1757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069026973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3870289", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070468202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2527/jas1988.6671686x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070902689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-002-0922-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075248697", 
          "https://doi.org/10.1007/s00425-002-0922-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1987.tb02538.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079934527", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081560015", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-07", 
    "datePublishedReg": "2007-07-01", 
    "description": "A mutant Bowman-Birk gene was created that encoded an inactive high-sulfur product. It was used to transform soybean line Asgrow 3237. Transformants bearing the mutant gene were identified by GUS expression, PCR analysis, and Southern analysis. The amount of steady state mRNA from the mutant gene in the transformed plants showed that the gene was highly expressed, but the amount of message from the unmodified Bowman-Birk gene did not change detectably. Proteins synthesized at the direction of the mutant Bowman-Birk gene accumulated in seeds of the transformed plants, and there was a marked decrease in the ability of extracts prepared from these seeds to inhibit trypsin and chymotrypsin despite the presence of Kunitz trypsin inhibitor. The more prevalent mRNA from the mutant gene was considered to out-compete message from the native genes to decrease the amount of active Bowman-Birk inhibitor.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11103-007-9163-x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "64"
      }
    ], 
    "name": "Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed", 
    "pagination": "397-408", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0e5f4ba9ffd24d80e2ef8f5af74b701c0cb57c47a8c3025048615456379d780f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17429741"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9106343"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11103-007-9163-x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1003017702"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11103-007-9163-x", 
      "https://app.dimensions.ai/details/publication/pub.1003017702"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000365_0000000365/records_71704_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11103-007-9163-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11103-007-9163-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11103-007-9163-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11103-007-9163-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11103-007-9163-x'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      78 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11103-007-9163-x schema:about N23688b31b5574ccf96590d291b76e5ab
2 N32933755088c48fbabefcb74b5303630
3 N3581978786504afcb3ab20b9b367daed
4 N36042ed9c25a4a5cac706df35bcc13ff
5 N47a0ca0fc7e94c2eb6ca6711953ae135
6 N663b4a80ba914207b1e6bc3e7c6a2a08
7 N75f90b6fcb7e4742b6748035d3ad51bd
8 Nac60b165ff1f40aaa879edab735ac1a6
9 Ncd3a45bc59b2447fbfd4cd61d3a6c86a
10 Nd29a7fce21454ce19ba5207f478ec72e
11 Nf3209ca8792b4c7ea5cf09111fc77b00
12 anzsrc-for:06
13 anzsrc-for:0604
14 schema:author Nd36cf1634347465988bb3ab8d417e050
15 schema:citation sg:pub.10.1007/978-1-4757-0022-0_1
16 sg:pub.10.1007/bf00260489
17 sg:pub.10.1007/bf02663049
18 sg:pub.10.1007/s00425-002-0922-2
19 sg:pub.10.1038/313810a0
20 sg:pub.10.1038/nbt0888-915
21 https://app.dimensions.ai/details/publication/pub.1079934527
22 https://app.dimensions.ai/details/publication/pub.1081560015
23 https://doi.org/10.1002/j.1460-2075.1987.tb02538.x
24 https://doi.org/10.1002/jsfa.2740330707
25 https://doi.org/10.1002/pmic.200500503
26 https://doi.org/10.1016/0003-2697(76)90527-3
27 https://doi.org/10.1016/0005-2795(77)90392-0
28 https://doi.org/10.1016/0378-1119(92)90157-k
29 https://doi.org/10.1017/s0021859699007443
30 https://doi.org/10.1021/bi00651a021
31 https://doi.org/10.1021/bi00837a032
32 https://doi.org/10.1021/jf00078a028
33 https://doi.org/10.1021/jf971086r
34 https://doi.org/10.1071/pp9780641
35 https://doi.org/10.1073/pnas.80.7.1897
36 https://doi.org/10.1073/pnas.94.16.8393
37 https://doi.org/10.1080/0007166031000085463
38 https://doi.org/10.1104/pp.000653
39 https://doi.org/10.1104/pp.104.056614
40 https://doi.org/10.1104/pp.74.3.576
41 https://doi.org/10.1104/pp.80.2.561
42 https://doi.org/10.1104/pp.81.3.802
43 https://doi.org/10.1105/tpc.1.11.1079
44 https://doi.org/10.1105/tpc.8.5.873
45 https://doi.org/10.1111/j.1399-3011.1985.tb02155.x
46 https://doi.org/10.1126/science.236.4806.1299
47 https://doi.org/10.1146/annurev.bi.49.070180.003113
48 https://doi.org/10.2135/cropsci1986.0011183x002600030058x
49 https://doi.org/10.2135/cropsci2001.1757
50 https://doi.org/10.2307/3869025
51 https://doi.org/10.2307/3870289
52 https://doi.org/10.2527/jas1988.6671686x
53 schema:datePublished 2007-07
54 schema:datePublishedReg 2007-07-01
55 schema:description A mutant Bowman-Birk gene was created that encoded an inactive high-sulfur product. It was used to transform soybean line Asgrow 3237. Transformants bearing the mutant gene were identified by GUS expression, PCR analysis, and Southern analysis. The amount of steady state mRNA from the mutant gene in the transformed plants showed that the gene was highly expressed, but the amount of message from the unmodified Bowman-Birk gene did not change detectably. Proteins synthesized at the direction of the mutant Bowman-Birk gene accumulated in seeds of the transformed plants, and there was a marked decrease in the ability of extracts prepared from these seeds to inhibit trypsin and chymotrypsin despite the presence of Kunitz trypsin inhibitor. The more prevalent mRNA from the mutant gene was considered to out-compete message from the native genes to decrease the amount of active Bowman-Birk inhibitor.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree false
59 schema:isPartOf N4da16f1d0404478f82aea0faa462e3a7
60 N644418d8c0884040a861fd0d7d4152e4
61 sg:journal.1101246
62 schema:name Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed
63 schema:pagination 397-408
64 schema:productId N883b2dd36eb04e7cb99b09589c2f9d24
65 N893f4385555f4a5fbfc1d839347fdcc9
66 Nb312ec9ce31e4cd4af40afeb2beed5c5
67 Nb9da7059db104b3c9b91a56e1aa30ffc
68 Ndcaf04fc711548b180412928d444475c
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003017702
70 https://doi.org/10.1007/s11103-007-9163-x
71 schema:sdDatePublished 2019-04-11T13:00
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher N5c56ec31fd13440283b6f153fc15fa85
74 schema:url http://link.springer.com/10.1007%2Fs11103-007-9163-x
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N124e180ea2a5463fa1813be04dc02e9c rdf:first sg:person.0610577775.27
79 rdf:rest N16c508c494304de893ca202b5bb75561
80 N16c508c494304de893ca202b5bb75561 rdf:first sg:person.01077053611.77
81 rdf:rest Nac2878b0e90044f19d3c3fb535d96c22
82 N23688b31b5574ccf96590d291b76e5ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Seeds
84 rdf:type schema:DefinedTerm
85 N32933755088c48fbabefcb74b5303630 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Soybeans
87 rdf:type schema:DefinedTerm
88 N3581978786504afcb3ab20b9b367daed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Amino Acid Sequence
90 rdf:type schema:DefinedTerm
91 N36042ed9c25a4a5cac706df35bcc13ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Base Sequence
93 rdf:type schema:DefinedTerm
94 N3af496d47ab6479496155b544a24c1d3 rdf:first sg:person.01030217545.28
95 rdf:rest rdf:nil
96 N47a0ca0fc7e94c2eb6ca6711953ae135 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name RNA, Messenger
98 rdf:type schema:DefinedTerm
99 N4da16f1d0404478f82aea0faa462e3a7 schema:issueNumber 4
100 rdf:type schema:PublicationIssue
101 N5c56ec31fd13440283b6f153fc15fa85 schema:name Springer Nature - SN SciGraph project
102 rdf:type schema:Organization
103 N644418d8c0884040a861fd0d7d4152e4 schema:volumeNumber 64
104 rdf:type schema:PublicationVolume
105 N663b4a80ba914207b1e6bc3e7c6a2a08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Trypsin Inhibitors
107 rdf:type schema:DefinedTerm
108 N66d5b6eabdb54852b6a3d326760b9734 rdf:first sg:person.0670015020.99
109 rdf:rest N124e180ea2a5463fa1813be04dc02e9c
110 N75f90b6fcb7e4742b6748035d3ad51bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Molecular Sequence Data
112 rdf:type schema:DefinedTerm
113 N883b2dd36eb04e7cb99b09589c2f9d24 schema:name pubmed_id
114 schema:value 17429741
115 rdf:type schema:PropertyValue
116 N893f4385555f4a5fbfc1d839347fdcc9 schema:name doi
117 schema:value 10.1007/s11103-007-9163-x
118 rdf:type schema:PropertyValue
119 Nac2878b0e90044f19d3c3fb535d96c22 rdf:first sg:person.01356203765.61
120 rdf:rest N3af496d47ab6479496155b544a24c1d3
121 Nac60b165ff1f40aaa879edab735ac1a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Trypsin Inhibitor, Kunitz Soybean
123 rdf:type schema:DefinedTerm
124 Nb312ec9ce31e4cd4af40afeb2beed5c5 schema:name readcube_id
125 schema:value 0e5f4ba9ffd24d80e2ef8f5af74b701c0cb57c47a8c3025048615456379d780f
126 rdf:type schema:PropertyValue
127 Nb9da7059db104b3c9b91a56e1aa30ffc schema:name nlm_unique_id
128 schema:value 9106343
129 rdf:type schema:PropertyValue
130 Ncd3a45bc59b2447fbfd4cd61d3a6c86a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Mutation
132 rdf:type schema:DefinedTerm
133 Nd29a7fce21454ce19ba5207f478ec72e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Sequence Alignment
135 rdf:type schema:DefinedTerm
136 Nd36cf1634347465988bb3ab8d417e050 rdf:first sg:person.01370756605.05
137 rdf:rest N66d5b6eabdb54852b6a3d326760b9734
138 Ndcaf04fc711548b180412928d444475c schema:name dimensions_id
139 schema:value pub.1003017702
140 rdf:type schema:PropertyValue
141 Nf3209ca8792b4c7ea5cf09111fc77b00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Trypsin Inhibitor, Bowman-Birk Soybean
143 rdf:type schema:DefinedTerm
144 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
145 schema:name Biological Sciences
146 rdf:type schema:DefinedTerm
147 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
148 schema:name Genetics
149 rdf:type schema:DefinedTerm
150 sg:journal.1101246 schema:issn 0167-4412
151 1573-5028
152 schema:name Plant Molecular Biology
153 rdf:type schema:Periodical
154 sg:person.01030217545.28 schema:affiliation https://www.grid.ac/institutes/grid.40803.3f
155 schema:familyName Nielsen
156 schema:givenName Niels C.
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01030217545.28
158 rdf:type schema:Person
159 sg:person.01077053611.77 schema:affiliation https://www.grid.ac/institutes/grid.34424.35
160 schema:familyName Schmidt
161 schema:givenName Monica A.
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077053611.77
163 rdf:type schema:Person
164 sg:person.01356203765.61 schema:affiliation https://www.grid.ac/institutes/grid.34424.35
165 schema:familyName Herman
166 schema:givenName Eliot M.
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01356203765.61
168 rdf:type schema:Person
169 sg:person.01370756605.05 schema:affiliation https://www.grid.ac/institutes/grid.169077.e
170 schema:familyName Livingstone
171 schema:givenName Donald
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01370756605.05
173 rdf:type schema:Person
174 sg:person.0610577775.27 schema:affiliation https://www.grid.ac/institutes/grid.451298.3
175 schema:familyName Kalyaeva
176 schema:givenName Marina
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610577775.27
178 rdf:type schema:Person
179 sg:person.0670015020.99 schema:affiliation https://www.grid.ac/institutes/grid.169077.e
180 schema:familyName Beilinson
181 schema:givenName Vadim
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670015020.99
183 rdf:type schema:Person
184 sg:pub.10.1007/978-1-4757-0022-0_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039643970
185 https://doi.org/10.1007/978-1-4757-0022-0_1
186 rdf:type schema:CreativeWork
187 sg:pub.10.1007/bf00260489 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047369882
188 https://doi.org/10.1007/bf00260489
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/bf02663049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035873596
191 https://doi.org/10.1007/bf02663049
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/s00425-002-0922-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075248697
194 https://doi.org/10.1007/s00425-002-0922-2
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/313810a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035513087
197 https://doi.org/10.1038/313810a0
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/nbt0888-915 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043462937
200 https://doi.org/10.1038/nbt0888-915
201 rdf:type schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1079934527 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1081560015 schema:CreativeWork
204 https://doi.org/10.1002/j.1460-2075.1987.tb02538.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183944
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1002/jsfa.2740330707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045472089
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1002/pmic.200500503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043915885
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0005-2795(77)90392-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009535433
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0378-1119(92)90157-k schema:sameAs https://app.dimensions.ai/details/publication/pub.1050848607
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1017/s0021859699007443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054075133
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1021/bi00651a021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055184075
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1021/bi00837a032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055190247
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1021/jf00078a028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055895442
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1021/jf971086r schema:sameAs https://app.dimensions.ai/details/publication/pub.1055930076
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1071/pp9780641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050813146
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1073/pnas.80.7.1897 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011917250
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.94.16.8393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019659434
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1080/0007166031000085463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018361618
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1104/pp.000653 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020010270
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1104/pp.104.056614 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039980918
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1104/pp.74.3.576 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001599135
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1104/pp.80.2.561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037221519
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1104/pp.81.3.802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023050999
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1105/tpc.1.11.1079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030259842
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1105/tpc.8.5.873 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041586080
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1111/j.1399-3011.1985.tb02155.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019116996
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1126/science.236.4806.1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062534239
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1146/annurev.bi.49.070180.003113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042085921
253 rdf:type schema:CreativeWork
254 https://doi.org/10.2135/cropsci1986.0011183x002600030058x schema:sameAs https://app.dimensions.ai/details/publication/pub.1069020883
255 rdf:type schema:CreativeWork
256 https://doi.org/10.2135/cropsci2001.1757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069026973
257 rdf:type schema:CreativeWork
258 https://doi.org/10.2307/3869025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467113
259 rdf:type schema:CreativeWork
260 https://doi.org/10.2307/3870289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070468202
261 rdf:type schema:CreativeWork
262 https://doi.org/10.2527/jas1988.6671686x schema:sameAs https://app.dimensions.ai/details/publication/pub.1070902689
263 rdf:type schema:CreativeWork
264 https://www.grid.ac/institutes/grid.169077.e schema:alternateName Purdue University
265 schema:name Athenix Corporation, 2202 Ellis Rd, 27703, Durham, NC, USA
266 Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA
267 Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA
268 rdf:type schema:Organization
269 https://www.grid.ac/institutes/grid.34424.35 schema:alternateName Donald Danforth Plant Science Center
270 schema:name Agricultural Research Service, United States Department of Agriculture, St. Louis, USA
271 Donald Danforth Plant Science Center, 975 Warson Rd, 63132, St. Louis, MO, USA
272 rdf:type schema:Organization
273 https://www.grid.ac/institutes/grid.40803.3f schema:alternateName North Carolina State University
274 schema:name Agricultural Research Service, United States Department of Agriculture, West Lafayette, USA
275 Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA
276 Department of Crop Science, North Carolina State University, 27695, Raleigh, NC, USA
277 rdf:type schema:Organization
278 https://www.grid.ac/institutes/grid.451298.3 schema:alternateName Simplot (United States)
279 schema:name Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA
280 J.R. Simplot Company, 999 Main St., Suite 1300, 83702, Boise, ID, USA
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...