Identification of a Nicotiana plumbaginifolia Plasma Membrane H+-ATPase Gene Expressed in the Pollen Tube View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-08

AUTHORS

Benoit Lefebvre, Miguel Arango, Mohammed Oufattole, Jérôme Crouzet, Bénédicte Purnelle, Marc Boutry

ABSTRACT

In Nicotiana plumbaginifolia, plasma membrane H(+)-ATPases (PMAs) are encoded by a gene family of nine members. Here, we report on the characterization of a new isogene, NpPMA5 (belonging to subfamily IV), and the determination of its expression pattern using the beta-glucuronidase (gusA) reporter gene. pNpPMA5-gusA was expressed in cotyledons, in vascular tissues of the stem (mainly in nodal zones), and in the flower and fruit. In the flower, high expression was found in the pollen tube after in vitro or in vivo germination. Northern blotting analysis confirmed that NpPMA5 was expressed in the pollen tube contrary to NpPMA2 (subfamily I) or NpPMA4 (subfamily II), two genes highly expressed in other tissues. The subcellular localization of PM H(+)-ATPase in the pollen tube was analyzed by immunocytodecoration. As expected, this enzyme was localized to the plasma membrane. However, neither the tip nor the base of the pollen tube was labeled, showing an asymmetrical distribution of this enzyme. This observation supports the hypothesis that the PM H(+)-ATPase is involved in creating the pH gradient that is observed along the pollen tube and is implicated in cell elongation. Compared to other plant PM H(+)-ATPases, the C-terminal region of NpPMA5 is shorter by 26 amino acid residues and is modified in the last 6 residues, due to a sequence rearrangement, which was also found in the orthologous gene of Nicotiana glutinosa, a Nicotiana species distant from N. plumbaginifolia and Petunia hybrida and Lycopersicon esculentum, other Solanacae species. This modification alters part of the PM H(+)-ATPase regulatory domain and raises the question whether this isoform is still regulated. More... »

PAGES

775-787

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11103-005-7875-3

DOI

http://dx.doi.org/10.1007/s11103-005-7875-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019943935

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16240173


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flowers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Enzymologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organ Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phylogeny", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proton-Translocating ATPases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Alignment", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Catholique de Louvain", 
          "id": "https://www.grid.ac/institutes/grid.7942.8", 
          "name": [
            "Unit\u00e9 de biochimie physiologique, Institut des sciences de la vie, Universit\u00e9 catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lefebvre", 
        "givenName": "Benoit", 
        "id": "sg:person.01002711234.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002711234.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Catholique de Louvain", 
          "id": "https://www.grid.ac/institutes/grid.7942.8", 
          "name": [
            "Unit\u00e9 de biochimie physiologique, Institut des sciences de la vie, Universit\u00e9 catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arango", 
        "givenName": "Miguel", 
        "id": "sg:person.01312225336.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312225336.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Catholique de Louvain", 
          "id": "https://www.grid.ac/institutes/grid.7942.8", 
          "name": [
            "Unit\u00e9 de biochimie physiologique, Institut des sciences de la vie, Universit\u00e9 catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oufattole", 
        "givenName": "Mohammed", 
        "id": "sg:person.014327766374.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014327766374.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Catholique de Louvain", 
          "id": "https://www.grid.ac/institutes/grid.7942.8", 
          "name": [
            "Unit\u00e9 de biochimie physiologique, Institut des sciences de la vie, Universit\u00e9 catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crouzet", 
        "givenName": "J\u00e9r\u00f4me", 
        "id": "sg:person.01312331560.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312331560.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Catholique de Louvain", 
          "id": "https://www.grid.ac/institutes/grid.7942.8", 
          "name": [
            "Unit\u00e9 de biochimie physiologique, Institut des sciences de la vie, Universit\u00e9 catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Purnelle", 
        "givenName": "B\u00e9n\u00e9dicte", 
        "id": "sg:person.0613371706.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613371706.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universit\u00e9 Catholique de Louvain", 
          "id": "https://www.grid.ac/institutes/grid.7942.8", 
          "name": [
            "Unit\u00e9 de biochimie physiologique, Institut des sciences de la vie, Universit\u00e9 catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boutry", 
        "givenName": "Marc", 
        "id": "sg:person.01156422660.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156422660.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.83.8.2571", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003239205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.144.3.483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003295722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004250000483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004296282", 
          "https://doi.org/10.1007/s004250000483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1998.00266.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005680180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m909690199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007851132"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.1992.00405.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014805777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1521-1878(200101)23:1<86::aid-bies1011>3.0.co;2-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015201217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.275.14.9919", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018753606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1469-8137.2003.00847.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021312912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.119.2.627", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022425493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.arplant.52.1.817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022638611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/newbio244029a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022855491", 
          "https://doi.org/10.1038/newbio244029a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1999.00495.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025450997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.7.10.1655", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026274677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.5.2.159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028081083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.51.36774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028336787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1399-3054.1996.980209.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028344864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.40.23828", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028377432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.118.2.551", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028710071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m007740200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031674398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01379280", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034751089", 
          "https://doi.org/10.1007/bf01379280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01379280", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034751089", 
          "https://doi.org/10.1007/bf01379280"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.104.032326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035355727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.104.032326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035355727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.6.10.1375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038065127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/22.22.4673", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042438223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01280244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043430337", 
          "https://doi.org/10.1007/bf01280244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01280244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043430337", 
          "https://doi.org/10.1007/bf01280244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.022277", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046541791"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.103.021923", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047302005"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0079-6603(03)01014-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047433941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s004250050672", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049493213", 
          "https://doi.org/10.1007/s004250050672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.1991.00121.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052134694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi982482l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055216920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi982482l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055216920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869582", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3869975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2307/3870027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070467970"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-002-0856-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075215532", 
          "https://doi.org/10.1007/s00425-002-0856-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077620783", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079425218", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00936.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082990741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083105209", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083132473", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-08", 
    "datePublishedReg": "2005-08-01", 
    "description": "In Nicotiana plumbaginifolia, plasma membrane H(+)-ATPases (PMAs) are encoded by a gene family of nine members. Here, we report on the characterization of a new isogene, NpPMA5 (belonging to subfamily IV), and the determination of its expression pattern using the beta-glucuronidase (gusA) reporter gene. pNpPMA5-gusA was expressed in cotyledons, in vascular tissues of the stem (mainly in nodal zones), and in the flower and fruit. In the flower, high expression was found in the pollen tube after in vitro or in vivo germination. Northern blotting analysis confirmed that NpPMA5 was expressed in the pollen tube contrary to NpPMA2 (subfamily I) or NpPMA4 (subfamily II), two genes highly expressed in other tissues. The subcellular localization of PM H(+)-ATPase in the pollen tube was analyzed by immunocytodecoration. As expected, this enzyme was localized to the plasma membrane. However, neither the tip nor the base of the pollen tube was labeled, showing an asymmetrical distribution of this enzyme. This observation supports the hypothesis that the PM H(+)-ATPase is involved in creating the pH gradient that is observed along the pollen tube and is implicated in cell elongation. Compared to other plant PM H(+)-ATPases, the C-terminal region of NpPMA5 is shorter by 26 amino acid residues and is modified in the last 6 residues, due to a sequence rearrangement, which was also found in the orthologous gene of Nicotiana glutinosa, a Nicotiana species distant from N. plumbaginifolia and Petunia hybrida and Lycopersicon esculentum, other Solanacae species. This modification alters part of the PM H(+)-ATPase regulatory domain and raises the question whether this isoform is still regulated.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11103-005-7875-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "58"
      }
    ], 
    "name": "Identification of a Nicotiana plumbaginifolia Plasma Membrane H+-ATPase Gene Expressed in the Pollen Tube", 
    "pagination": "775-787", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5f06361cfba52c10fb41ce7908881cab44aebd3b1b85c13e317cecef8380f932"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16240173"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9106343"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11103-005-7875-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1019943935"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11103-005-7875-3", 
      "https://app.dimensions.ai/details/publication/pub.1019943935"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs11103-005-7875-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11103-005-7875-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11103-005-7875-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11103-005-7875-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11103-005-7875-3'


 

This table displays all metadata directly associated to this object as RDF triples.

274 TRIPLES      21 PREDICATES      81 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11103-005-7875-3 schema:about N04dae0616f6047dd8edcd29db36ebb25
2 N0e28cddf61a548cdb338322b665da4d4
3 N1d9a4272373b497baf303a42d6a6f7bb
4 N3397bb9b8097482fbc7431ddb40d5a3c
5 N3c10ac9af0bd407c91894c3f397f2436
6 N60a12a8cf9884c95871cac8cf2ebbc92
7 N67e2e48765ad4e37b2276145b7fca727
8 N722163a3df984818b25f0c2cb356e4d3
9 N81994691034b4f59b118a4c646697bb0
10 N97a71565426e4d66ad94d9563b143c30
11 Nd8193c76f78e4461afa8515ed58143a3
12 Ne80e833e2a434f338d7883ed22d48375
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author Ne26a2f46f8ba4b72bd207d67b7828045
16 schema:citation sg:pub.10.1007/bf01280244
17 sg:pub.10.1007/bf01379280
18 sg:pub.10.1007/s00425-002-0856-8
19 sg:pub.10.1007/s004250000483
20 sg:pub.10.1007/s004250050672
21 sg:pub.10.1038/newbio244029a0
22 https://app.dimensions.ai/details/publication/pub.1077620783
23 https://app.dimensions.ai/details/publication/pub.1079425218
24 https://app.dimensions.ai/details/publication/pub.1083105209
25 https://app.dimensions.ai/details/publication/pub.1083132473
26 https://doi.org/10.1002/1521-1878(200101)23:1<86::aid-bies1011>3.0.co;2-d
27 https://doi.org/10.1002/j.1460-2075.1996.tb00936.x
28 https://doi.org/10.1016/s0079-6603(03)01014-6
29 https://doi.org/10.1021/bi982482l
30 https://doi.org/10.1034/j.1399-3054.1996.980209.x
31 https://doi.org/10.1046/j.1365-313x.1998.00266.x
32 https://doi.org/10.1046/j.1365-313x.1999.00495.x
33 https://doi.org/10.1046/j.1469-8137.2003.00847.x
34 https://doi.org/10.1073/pnas.83.8.2571
35 https://doi.org/10.1074/jbc.270.40.23828
36 https://doi.org/10.1074/jbc.274.51.36774
37 https://doi.org/10.1074/jbc.275.14.9919
38 https://doi.org/10.1074/jbc.m007740200
39 https://doi.org/10.1074/jbc.m909690199
40 https://doi.org/10.1083/jcb.144.3.483
41 https://doi.org/10.1093/nar/22.22.4673
42 https://doi.org/10.1104/pp.103.021923
43 https://doi.org/10.1104/pp.118.2.551
44 https://doi.org/10.1104/pp.119.2.627
45 https://doi.org/10.1105/tpc.022277
46 https://doi.org/10.1105/tpc.5.2.159
47 https://doi.org/10.1105/tpc.6.10.1375
48 https://doi.org/10.1105/tpc.7.10.1655
49 https://doi.org/10.1111/j.1365-313x.1991.00121.x
50 https://doi.org/10.1111/j.1365-313x.1992.00405.x
51 https://doi.org/10.1146/annurev.arplant.52.1.817
52 https://doi.org/10.1534/genetics.104.032326
53 https://doi.org/10.2307/3869582
54 https://doi.org/10.2307/3869975
55 https://doi.org/10.2307/3870027
56 schema:datePublished 2005-08
57 schema:datePublishedReg 2005-08-01
58 schema:description In Nicotiana plumbaginifolia, plasma membrane H(+)-ATPases (PMAs) are encoded by a gene family of nine members. Here, we report on the characterization of a new isogene, NpPMA5 (belonging to subfamily IV), and the determination of its expression pattern using the beta-glucuronidase (gusA) reporter gene. pNpPMA5-gusA was expressed in cotyledons, in vascular tissues of the stem (mainly in nodal zones), and in the flower and fruit. In the flower, high expression was found in the pollen tube after in vitro or in vivo germination. Northern blotting analysis confirmed that NpPMA5 was expressed in the pollen tube contrary to NpPMA2 (subfamily I) or NpPMA4 (subfamily II), two genes highly expressed in other tissues. The subcellular localization of PM H(+)-ATPase in the pollen tube was analyzed by immunocytodecoration. As expected, this enzyme was localized to the plasma membrane. However, neither the tip nor the base of the pollen tube was labeled, showing an asymmetrical distribution of this enzyme. This observation supports the hypothesis that the PM H(+)-ATPase is involved in creating the pH gradient that is observed along the pollen tube and is implicated in cell elongation. Compared to other plant PM H(+)-ATPases, the C-terminal region of NpPMA5 is shorter by 26 amino acid residues and is modified in the last 6 residues, due to a sequence rearrangement, which was also found in the orthologous gene of Nicotiana glutinosa, a Nicotiana species distant from N. plumbaginifolia and Petunia hybrida and Lycopersicon esculentum, other Solanacae species. This modification alters part of the PM H(+)-ATPase regulatory domain and raises the question whether this isoform is still regulated.
59 schema:genre research_article
60 schema:inLanguage en
61 schema:isAccessibleForFree false
62 schema:isPartOf N382e6726c95f450ab52ad10503e2b274
63 N8134aed74cbb441ea278c374b3fc12c7
64 sg:journal.1101246
65 schema:name Identification of a Nicotiana plumbaginifolia Plasma Membrane H+-ATPase Gene Expressed in the Pollen Tube
66 schema:pagination 775-787
67 schema:productId N38a34c720c6849dd96873c47f4c5c27e
68 N5563c9eb25374d5b8cad26a9bfaa630d
69 N6a4ebdc81eab4094ba1c5f875e584ed8
70 Nc9162563f7334d93b7b9f1ce255a75f1
71 Ne0e9885ce9b1418ea6cd99fa42e59e5c
72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019943935
73 https://doi.org/10.1007/s11103-005-7875-3
74 schema:sdDatePublished 2019-04-11T13:47
75 schema:sdLicense https://scigraph.springernature.com/explorer/license/
76 schema:sdPublisher N88624b2325ac47cd8afa3b8a894bbba2
77 schema:url https://link.springer.com/10.1007%2Fs11103-005-7875-3
78 sgo:license sg:explorer/license/
79 sgo:sdDataset articles
80 rdf:type schema:ScholarlyArticle
81 N00fa30f5fde240c794bcd8715260cbf1 rdf:first sg:person.01156422660.48
82 rdf:rest rdf:nil
83 N028a20706e6345faa6d9bcd802c8f669 rdf:first sg:person.01312331560.26
84 rdf:rest Nddabaf25b6c14e07893395c6ee7cd639
85 N04dae0616f6047dd8edcd29db36ebb25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Gene Expression Regulation, Enzymologic
87 rdf:type schema:DefinedTerm
88 N0e28cddf61a548cdb338322b665da4d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Sequence Alignment
90 rdf:type schema:DefinedTerm
91 N1d9a4272373b497baf303a42d6a6f7bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Organ Specificity
93 rdf:type schema:DefinedTerm
94 N3397bb9b8097482fbc7431ddb40d5a3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Molecular Sequence Data
96 rdf:type schema:DefinedTerm
97 N382e6726c95f450ab52ad10503e2b274 schema:volumeNumber 58
98 rdf:type schema:PublicationVolume
99 N38a34c720c6849dd96873c47f4c5c27e schema:name pubmed_id
100 schema:value 16240173
101 rdf:type schema:PropertyValue
102 N3c10ac9af0bd407c91894c3f397f2436 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Gene Expression Regulation, Plant
104 rdf:type schema:DefinedTerm
105 N5563c9eb25374d5b8cad26a9bfaa630d schema:name dimensions_id
106 schema:value pub.1019943935
107 rdf:type schema:PropertyValue
108 N60a12a8cf9884c95871cac8cf2ebbc92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Flowers
110 rdf:type schema:DefinedTerm
111 N67e2e48765ad4e37b2276145b7fca727 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Base Sequence
113 rdf:type schema:DefinedTerm
114 N6a4ebdc81eab4094ba1c5f875e584ed8 schema:name doi
115 schema:value 10.1007/s11103-005-7875-3
116 rdf:type schema:PropertyValue
117 N722163a3df984818b25f0c2cb356e4d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Cell Membrane
119 rdf:type schema:DefinedTerm
120 N8134aed74cbb441ea278c374b3fc12c7 schema:issueNumber 6
121 rdf:type schema:PublicationIssue
122 N81994691034b4f59b118a4c646697bb0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Proton-Translocating ATPases
124 rdf:type schema:DefinedTerm
125 N88624b2325ac47cd8afa3b8a894bbba2 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 N97a71565426e4d66ad94d9563b143c30 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Phylogeny
129 rdf:type schema:DefinedTerm
130 Nc614b547d0004850973a15abe8c47ed6 rdf:first sg:person.014327766374.38
131 rdf:rest N028a20706e6345faa6d9bcd802c8f669
132 Nc9162563f7334d93b7b9f1ce255a75f1 schema:name nlm_unique_id
133 schema:value 9106343
134 rdf:type schema:PropertyValue
135 Nd8193c76f78e4461afa8515ed58143a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Sequence Homology, Nucleic Acid
137 rdf:type schema:DefinedTerm
138 Nddabaf25b6c14e07893395c6ee7cd639 rdf:first sg:person.0613371706.57
139 rdf:rest N00fa30f5fde240c794bcd8715260cbf1
140 Ne0e9885ce9b1418ea6cd99fa42e59e5c schema:name readcube_id
141 schema:value 5f06361cfba52c10fb41ce7908881cab44aebd3b1b85c13e317cecef8380f932
142 rdf:type schema:PropertyValue
143 Ne26a2f46f8ba4b72bd207d67b7828045 rdf:first sg:person.01002711234.11
144 rdf:rest Ne2884f9a252142c9bc46ac6a1605995a
145 Ne2884f9a252142c9bc46ac6a1605995a rdf:first sg:person.01312225336.15
146 rdf:rest Nc614b547d0004850973a15abe8c47ed6
147 Ne80e833e2a434f338d7883ed22d48375 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Tobacco
149 rdf:type schema:DefinedTerm
150 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
151 schema:name Biological Sciences
152 rdf:type schema:DefinedTerm
153 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
154 schema:name Genetics
155 rdf:type schema:DefinedTerm
156 sg:journal.1101246 schema:issn 0167-4412
157 1573-5028
158 schema:name Plant Molecular Biology
159 rdf:type schema:Periodical
160 sg:person.01002711234.11 schema:affiliation https://www.grid.ac/institutes/grid.7942.8
161 schema:familyName Lefebvre
162 schema:givenName Benoit
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002711234.11
164 rdf:type schema:Person
165 sg:person.01156422660.48 schema:affiliation https://www.grid.ac/institutes/grid.7942.8
166 schema:familyName Boutry
167 schema:givenName Marc
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156422660.48
169 rdf:type schema:Person
170 sg:person.01312225336.15 schema:affiliation https://www.grid.ac/institutes/grid.7942.8
171 schema:familyName Arango
172 schema:givenName Miguel
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312225336.15
174 rdf:type schema:Person
175 sg:person.01312331560.26 schema:affiliation https://www.grid.ac/institutes/grid.7942.8
176 schema:familyName Crouzet
177 schema:givenName Jérôme
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312331560.26
179 rdf:type schema:Person
180 sg:person.014327766374.38 schema:affiliation https://www.grid.ac/institutes/grid.7942.8
181 schema:familyName Oufattole
182 schema:givenName Mohammed
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014327766374.38
184 rdf:type schema:Person
185 sg:person.0613371706.57 schema:affiliation https://www.grid.ac/institutes/grid.7942.8
186 schema:familyName Purnelle
187 schema:givenName Bénédicte
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613371706.57
189 rdf:type schema:Person
190 sg:pub.10.1007/bf01280244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043430337
191 https://doi.org/10.1007/bf01280244
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/bf01379280 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034751089
194 https://doi.org/10.1007/bf01379280
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/s00425-002-0856-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075215532
197 https://doi.org/10.1007/s00425-002-0856-8
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/s004250000483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004296282
200 https://doi.org/10.1007/s004250000483
201 rdf:type schema:CreativeWork
202 sg:pub.10.1007/s004250050672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049493213
203 https://doi.org/10.1007/s004250050672
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/newbio244029a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022855491
206 https://doi.org/10.1038/newbio244029a0
207 rdf:type schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1077620783 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1079425218 schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1083105209 schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1083132473 schema:CreativeWork
212 https://doi.org/10.1002/1521-1878(200101)23:1<86::aid-bies1011>3.0.co;2-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1015201217
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1002/j.1460-2075.1996.tb00936.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082990741
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/s0079-6603(03)01014-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047433941
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1021/bi982482l schema:sameAs https://app.dimensions.ai/details/publication/pub.1055216920
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1034/j.1399-3054.1996.980209.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028344864
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1046/j.1365-313x.1998.00266.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005680180
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1046/j.1365-313x.1999.00495.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1025450997
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1046/j.1469-8137.2003.00847.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021312912
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1073/pnas.83.8.2571 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003239205
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1074/jbc.270.40.23828 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028377432
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1074/jbc.274.51.36774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028336787
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1074/jbc.275.14.9919 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018753606
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1074/jbc.m007740200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031674398
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1074/jbc.m909690199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007851132
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1083/jcb.144.3.483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003295722
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1093/nar/22.22.4673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042438223
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1104/pp.103.021923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047302005
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1104/pp.118.2.551 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028710071
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1104/pp.119.2.627 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022425493
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1105/tpc.022277 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046541791
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1105/tpc.5.2.159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028081083
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1105/tpc.6.10.1375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038065127
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1105/tpc.7.10.1655 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026274677
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1111/j.1365-313x.1991.00121.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052134694
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1111/j.1365-313x.1992.00405.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014805777
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1146/annurev.arplant.52.1.817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022638611
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1534/genetics.104.032326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035355727
265 rdf:type schema:CreativeWork
266 https://doi.org/10.2307/3869582 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467580
267 rdf:type schema:CreativeWork
268 https://doi.org/10.2307/3869975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467923
269 rdf:type schema:CreativeWork
270 https://doi.org/10.2307/3870027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070467970
271 rdf:type schema:CreativeWork
272 https://www.grid.ac/institutes/grid.7942.8 schema:alternateName Université Catholique de Louvain
273 schema:name Unité de biochimie physiologique, Institut des sciences de la vie, Université catholique de Louvain, Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium
274 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...