Oligodendrocytes Do Not Export NAA-Derived Aspartate In Vitro View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-03

AUTHORS

Ana I. Amaral, Mussie Ghezu Hadera, Mark Kotter, Ursula Sonnewald

ABSTRACT

Oligodendroglial cells are known to de-acetylate the N-acetylaspartate (NAA) synthesized and released by neurons and use it for lipid synthesis. However, the role of NAA regarding their intermediary metabolism remains poorly understood. Two hypotheses were proposed regarding the fate of aspartate after being released by de-acetylation: (1) aspartate is metabolized in the mitochondria of oligodendrocyte lineage cells; (2) aspartate is released to the medium. We report here that aspartoacylase mRNA expression increases when primary rat oligodendrocyte progenitor cells (OPCs) differentiate into mature cells in culture. Moreover, characterising metabolic functions of acetyl coenzyme A and aspartate from NAA catabolism in mature oligodendrocyte cultures after 5 days using isotope-labelled glucose after 5-days of differentiation we found evidence of extensive NAA metabolism. Incubation with [1,6-13C]glucose followed by gas chromatography-mass spectrometry and high performance liquid chromatography analyses of cell extracts and media in the presence and absence of NAA established that the acetate moiety produced by hydrolysis of NAA does not enter mitochondrial metabolism in the form of acetyl coenzyme A. We also resolved the controversy concerning the possible release of aspartate to the medium: aspartate is not released to the medium by oligodendrocytes in amounts detectable by our methods. Therefore we propose that: aspartate released from NAA joins the cytosolic aspartate pool rapidly and takes part in the malate-aspartate shuttle, which transports reducing equivalents from glycolysis into the mitochondria for ATP production and enters the tricarboxylic acid cycle at a slow rate. More... »

PAGES

827-837

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11064-016-1985-y

DOI

http://dx.doi.org/10.1007/s11064-016-1985-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048781360

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27394419


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11064-016-1985-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11064-016-1985-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11064-016-1985-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11064-016-1985-y'


 

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297 Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), MTFS, PO Box 8905, 7491, Trondheim, Norway
298 rdf:type schema:Organization
299 https://www.grid.ac/institutes/grid.5335.0 schema:alternateName University of Cambridge
300 schema:name Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, Anne McLaren Laboratory and Department of Clinical Neurosciences, University of Cambridge, West Forvie Building, Robinson Way, CB2 0SZ, Cambridge, UK
301 rdf:type schema:Organization
 




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