Effects of a Propolis Extract on the Viability of and Levels of Cytoskeletal and Regulatory Proteins in Rat Brain Astrocytes: ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-08

AUTHORS

C. A. Agca, A. A. Tykhomyrov, G. Baydas, V. S. Nedzvetsky

ABSTRACT

A potential for the use of propolis in preventive and therapeutic purposes has been acknowledged, but little attention has been paid to estimation of possible propolis cytotoxicity with respect to astrocytes. We tried to estimate how a propolis ethanol extract (PEE) affects rat brain astrocytes in vitro and also to uncover crucial molecular targets of the PEE action. Primary astrocytes were exposed to PEE in doses of 10, 25, or 100 μg/ml for 24 h, and then the cell viability was monitored by MTT assay. Levels of glial fibrillary acidic protein (GFAP), transcriptional nuclear factor-κB (NF-κB), poly(ADPribose) polymerase (PARP), and angiostatins were measured using Western blot to identify the molecular mechanisms underlying cell responses to PEE. The PEE treatment exerted a dose-dependent cytotoxic effect on cultured astrocytes. The PEE modulated astrocyte signaling pathways through inducing the expression of NF-κB and PARP. At the same time, the PEE stimulated GFAP synthesis and fibrillogenesis, which was indicative for activation of astrocytes preceding their suppression. The PEE significantly increased the production of angiostatin isoforms by astrocytes, thus contributing to an antiangiogenic potential of these cells. In summary, our results indicated that exposure to the PEE exerts certain cytotoxic effects on astrocytes in a dose-dependent manner; these effects are realized through modulation of cytoskeleton rearrangements and pro-apoptotic signaling pathways. A widely available, safe, and inexpensive substance, propolis, and its components and derivatives may be used in the prevention and treatment of neuronal impairments, including malignant tumors and neurodegenerative disorders associated with excessive astrocytic activation. More... »

PAGES

261-271

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11062-017-9680-4

DOI

http://dx.doi.org/10.1007/s11062-017-9680-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1093096533


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Bing\u00f6l University", 
          "id": "https://www.grid.ac/institutes/grid.448543.a", 
          "name": [
            "Bing\u00f6l University, Bing\u00f6l, Turkey"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Agca", 
        "givenName": "C. A.", 
        "id": "sg:person.01307633263.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307633263.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tykhomyrov", 
        "givenName": "A. A.", 
        "id": "sg:person.011625744037.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011625744037.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Council of Higher Education", 
          "id": "https://www.grid.ac/institutes/grid.467929.7", 
          "name": [
            "Council of Higher Education, Ankara, Turkey"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baydas", 
        "givenName": "G.", 
        "id": "sg:person.0760776064.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760776064.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Bing\u00f6l University", 
          "id": "https://www.grid.ac/institutes/grid.448543.a", 
          "name": [
            "Bing\u00f6l University, Bing\u00f6l, Turkey", 
            "Oles\u2019 Honchar Dnipro National University, Dnipro, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nedzvetsky", 
        "givenName": "V. S.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.jep.2005.05.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001975493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jep.2005.05.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001975493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ptr.5605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002803433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jnc.13423", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003561894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.20480", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003795584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.20480", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003795584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/mmr.2016.4948", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008922060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/mmr.2016.4948", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008922060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1472-6882-9-45", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009920114", 
          "https://doi.org/10.1186/1472-6882-9-45"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11062-016-9568-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010336368", 
          "https://doi.org/10.1007/s11062-016-9568-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.2042-7158.2011.01331.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013086776"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jnc.12611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015442679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddt036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016159394"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2014.03.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017299042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1478-811x-8-31", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017427406", 
          "https://doi.org/10.1186/1478-811x-8-31"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibs.2009.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019867611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40478-016-0350-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021297844", 
          "https://doi.org/10.1186/s40478-016-0350-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40478-016-0350-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021297844", 
          "https://doi.org/10.1186/s40478-016-0350-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biopha.2016.06.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021361511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2009.07177.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022786421"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11064-016-1964-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024280031", 
          "https://doi.org/10.1007/s11064-016-1964-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11064-016-1964-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024280031", 
          "https://doi.org/10.1007/s11064-016-1964-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/molecules191219610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024394457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/157015911795596540", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024580607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm3376", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025478850", 
          "https://doi.org/10.1038/nrm3376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jphp.12628", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025543298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/onc.2015.210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025993546", 
          "https://doi.org/10.1038/onc.2015.210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.canlet.2006.01.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026964533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.fct.2010.09.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029865911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/ecam/neh078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030490300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2014/897361", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031256128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1046/j.1440-1711.1999.00823.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031731769", 
          "https://doi.org/10.1046/j.1440-1711.1999.00823.x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2012/176287", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034008169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0165588", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034106673"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/cshperspect.a001651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037119425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2013/964149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037590658"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1590/s1517-83822013005000062", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037879694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc3748", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038014944", 
          "https://doi.org/10.1038/nrc3748"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-230x(00)79001-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038853826"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12906-015-0677-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039408033", 
          "https://doi.org/10.1186/s12906-015-0677-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12906-015-0677-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039408033", 
          "https://doi.org/10.1186/s12906-015-0677-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2014/145342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042182504"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.phymed.2014.04.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044568427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn1824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045889344", 
          "https://doi.org/10.1038/nrn1824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn1824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045889344", 
          "https://doi.org/10.1038/nrn1824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.5552-09.2010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047332867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuron.2013.12.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051681631"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0308-8146(03)00216-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052180548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0308-8146(03)00216-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052180548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-2001-7-828", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067583662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18097/pbmc20156101041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1068571412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1567205011310030004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069188049"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2478/v10039-012-0042-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070852163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/etm.2016.3070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071503995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/etm.2016.3070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071503995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.23-10-04034.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1075290601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079959685", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/celldisc.2017.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083885240", 
          "https://doi.org/10.1038/celldisc.2017.1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.15407/ubj89.02.043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085268717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biopha.2017.06.072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090346772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jphp.12789", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090995345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/bpa.12537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091208114"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-08", 
    "datePublishedReg": "2017-08-01", 
    "description": "A potential for the use of propolis in preventive and therapeutic purposes has been acknowledged, but little attention has been paid to estimation of possible propolis cytotoxicity with respect to astrocytes. We tried to estimate how a propolis ethanol extract (PEE) affects rat brain astrocytes in vitro and also to uncover crucial molecular targets of the PEE action. Primary astrocytes were exposed to PEE in doses of 10, 25, or 100 \u03bcg/ml for 24 h, and then the cell viability was monitored by MTT assay. Levels of glial fibrillary acidic protein (GFAP), transcriptional nuclear factor-\u03baB (NF-\u03baB), poly(ADPribose) polymerase (PARP), and angiostatins were measured using Western blot to identify the molecular mechanisms underlying cell responses to PEE. The PEE treatment exerted a dose-dependent cytotoxic effect on cultured astrocytes. The PEE modulated astrocyte signaling pathways through inducing the expression of NF-\u03baB and PARP. At the same time, the PEE stimulated GFAP synthesis and fibrillogenesis, which was indicative for activation of astrocytes preceding their suppression. The PEE significantly increased the production of angiostatin isoforms by astrocytes, thus contributing to an antiangiogenic potential of these cells. In summary, our results indicated that exposure to the PEE exerts certain cytotoxic effects on astrocytes in a dose-dependent manner; these effects are realized through modulation of cytoskeleton rearrangements and pro-apoptotic signaling pathways. A widely available, safe, and inexpensive substance, propolis, and its components and derivatives may be used in the prevention and treatment of neuronal impairments, including malignant tumors and neurodegenerative disorders associated with excessive astrocytic activation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11062-017-9680-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1016854", 
        "issn": [
          "0090-2977", 
          "1573-9007"
        ], 
        "name": "Neurophysiology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "49"
      }
    ], 
    "name": "Effects of a Propolis Extract on the Viability of and Levels of Cytoskeletal and Regulatory Proteins in Rat Brain Astrocytes: an In Vitro Study", 
    "pagination": "261-271", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d77f0d160ff87310e48fa176fac824daec89ddc672567ab6ed323e62708e80eb"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11062-017-9680-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1093096533"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11062-017-9680-4", 
      "https://app.dimensions.ai/details/publication/pub.1093096533"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000484.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s11062-017-9680-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11062-017-9680-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11062-017-9680-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11062-017-9680-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11062-017-9680-4'


 

This table displays all metadata directly associated to this object as RDF triples.

261 TRIPLES      21 PREDICATES      81 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11062-017-9680-4 schema:about anzsrc-for:11
2 anzsrc-for:1109
3 schema:author N63c4693006ca402aa01f0c5f6d2fa2d6
4 schema:citation sg:pub.10.1007/s11062-016-9568-8
5 sg:pub.10.1007/s11064-016-1964-3
6 sg:pub.10.1038/celldisc.2017.1
7 sg:pub.10.1038/nrc3748
8 sg:pub.10.1038/nrm3376
9 sg:pub.10.1038/nrn1824
10 sg:pub.10.1038/onc.2015.210
11 sg:pub.10.1046/j.1440-1711.1999.00823.x
12 sg:pub.10.1186/1472-6882-9-45
13 sg:pub.10.1186/1478-811x-8-31
14 sg:pub.10.1186/s12906-015-0677-5
15 sg:pub.10.1186/s40478-016-0350-3
16 https://app.dimensions.ai/details/publication/pub.1079959685
17 https://doi.org/10.1002/jcb.20480
18 https://doi.org/10.1002/ptr.5605
19 https://doi.org/10.1016/0003-2697(76)90527-3
20 https://doi.org/10.1016/j.biopha.2016.06.013
21 https://doi.org/10.1016/j.biopha.2017.06.072
22 https://doi.org/10.1016/j.canlet.2006.01.016
23 https://doi.org/10.1016/j.cub.2014.03.034
24 https://doi.org/10.1016/j.fct.2010.09.034
25 https://doi.org/10.1016/j.jep.2005.05.004
26 https://doi.org/10.1016/j.neuron.2013.12.034
27 https://doi.org/10.1016/j.phymed.2014.04.006
28 https://doi.org/10.1016/j.tibs.2009.12.003
29 https://doi.org/10.1016/s0065-230x(00)79001-4
30 https://doi.org/10.1016/s0308-8146(03)00216-4
31 https://doi.org/10.1093/ecam/neh078
32 https://doi.org/10.1093/hmg/ddt036
33 https://doi.org/10.1101/cshperspect.a001651
34 https://doi.org/10.1111/bpa.12537
35 https://doi.org/10.1111/j.1742-4658.2009.07177.x
36 https://doi.org/10.1111/j.2042-7158.2011.01331.x
37 https://doi.org/10.1111/jnc.12611
38 https://doi.org/10.1111/jnc.13423
39 https://doi.org/10.1111/jphp.12628
40 https://doi.org/10.1111/jphp.12789
41 https://doi.org/10.1155/2012/176287
42 https://doi.org/10.1155/2013/964149
43 https://doi.org/10.1155/2014/145342
44 https://doi.org/10.1155/2014/897361
45 https://doi.org/10.1371/journal.pone.0165588
46 https://doi.org/10.1515/znc-2001-7-828
47 https://doi.org/10.1523/jneurosci.23-10-04034.2003
48 https://doi.org/10.1523/jneurosci.5552-09.2010
49 https://doi.org/10.15407/ubj89.02.043
50 https://doi.org/10.1590/s1517-83822013005000062
51 https://doi.org/10.18097/pbmc20156101041
52 https://doi.org/10.2174/1567205011310030004
53 https://doi.org/10.2174/157015911795596540
54 https://doi.org/10.2478/v10039-012-0042-6
55 https://doi.org/10.3390/molecules191219610
56 https://doi.org/10.3892/etm.2016.3070
57 https://doi.org/10.3892/mmr.2016.4948
58 schema:datePublished 2017-08
59 schema:datePublishedReg 2017-08-01
60 schema:description A potential for the use of propolis in preventive and therapeutic purposes has been acknowledged, but little attention has been paid to estimation of possible propolis cytotoxicity with respect to astrocytes. We tried to estimate how a propolis ethanol extract (PEE) affects rat brain astrocytes in vitro and also to uncover crucial molecular targets of the PEE action. Primary astrocytes were exposed to PEE in doses of 10, 25, or 100 μg/ml for 24 h, and then the cell viability was monitored by MTT assay. Levels of glial fibrillary acidic protein (GFAP), transcriptional nuclear factor-κB (NF-κB), poly(ADPribose) polymerase (PARP), and angiostatins were measured using Western blot to identify the molecular mechanisms underlying cell responses to PEE. The PEE treatment exerted a dose-dependent cytotoxic effect on cultured astrocytes. The PEE modulated astrocyte signaling pathways through inducing the expression of NF-κB and PARP. At the same time, the PEE stimulated GFAP synthesis and fibrillogenesis, which was indicative for activation of astrocytes preceding their suppression. The PEE significantly increased the production of angiostatin isoforms by astrocytes, thus contributing to an antiangiogenic potential of these cells. In summary, our results indicated that exposure to the PEE exerts certain cytotoxic effects on astrocytes in a dose-dependent manner; these effects are realized through modulation of cytoskeleton rearrangements and pro-apoptotic signaling pathways. A widely available, safe, and inexpensive substance, propolis, and its components and derivatives may be used in the prevention and treatment of neuronal impairments, including malignant tumors and neurodegenerative disorders associated with excessive astrocytic activation.
61 schema:genre research_article
62 schema:inLanguage en
63 schema:isAccessibleForFree false
64 schema:isPartOf Ncfaaa7ab6e9d4725bc42e313bbd3abcc
65 Nfa598de30b744816be9fe55b9545526b
66 sg:journal.1016854
67 schema:name Effects of a Propolis Extract on the Viability of and Levels of Cytoskeletal and Regulatory Proteins in Rat Brain Astrocytes: an In Vitro Study
68 schema:pagination 261-271
69 schema:productId N8ed5d83aee224c908baa4388f60b89ca
70 Nb502a51c58fb4fdf91e3f0098aa2f8d1
71 Nf624cd38fff94d538b6a7d53b582c09b
72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093096533
73 https://doi.org/10.1007/s11062-017-9680-4
74 schema:sdDatePublished 2019-04-11T01:01
75 schema:sdLicense https://scigraph.springernature.com/explorer/license/
76 schema:sdPublisher Nd6f4c0ea38614c3587cf4a5d125a7257
77 schema:url http://link.springer.com/10.1007/s11062-017-9680-4
78 sgo:license sg:explorer/license/
79 sgo:sdDataset articles
80 rdf:type schema:ScholarlyArticle
81 N06160d9f1bb649d49d4bf8746706452a rdf:first sg:person.011625744037.33
82 rdf:rest N51f0a1544d8046978215000722ff4c7e
83 N51f0a1544d8046978215000722ff4c7e rdf:first sg:person.0760776064.95
84 rdf:rest N645fc46665b545e983f09660f815cbce
85 N63c4693006ca402aa01f0c5f6d2fa2d6 rdf:first sg:person.01307633263.55
86 rdf:rest N06160d9f1bb649d49d4bf8746706452a
87 N645fc46665b545e983f09660f815cbce rdf:first Nbbfd307d812c4c95a50ec484e98e91d6
88 rdf:rest rdf:nil
89 N8ed5d83aee224c908baa4388f60b89ca schema:name readcube_id
90 schema:value d77f0d160ff87310e48fa176fac824daec89ddc672567ab6ed323e62708e80eb
91 rdf:type schema:PropertyValue
92 Nb502a51c58fb4fdf91e3f0098aa2f8d1 schema:name doi
93 schema:value 10.1007/s11062-017-9680-4
94 rdf:type schema:PropertyValue
95 Nbbfd307d812c4c95a50ec484e98e91d6 schema:affiliation https://www.grid.ac/institutes/grid.448543.a
96 schema:familyName Nedzvetsky
97 schema:givenName V. S.
98 rdf:type schema:Person
99 Ncfaaa7ab6e9d4725bc42e313bbd3abcc schema:issueNumber 4
100 rdf:type schema:PublicationIssue
101 Nd6f4c0ea38614c3587cf4a5d125a7257 schema:name Springer Nature - SN SciGraph project
102 rdf:type schema:Organization
103 Nf624cd38fff94d538b6a7d53b582c09b schema:name dimensions_id
104 schema:value pub.1093096533
105 rdf:type schema:PropertyValue
106 Nfa598de30b744816be9fe55b9545526b schema:volumeNumber 49
107 rdf:type schema:PublicationVolume
108 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
109 schema:name Medical and Health Sciences
110 rdf:type schema:DefinedTerm
111 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
112 schema:name Neurosciences
113 rdf:type schema:DefinedTerm
114 sg:journal.1016854 schema:issn 0090-2977
115 1573-9007
116 schema:name Neurophysiology
117 rdf:type schema:Periodical
118 sg:person.011625744037.33 schema:affiliation https://www.grid.ac/institutes/grid.419966.5
119 schema:familyName Tykhomyrov
120 schema:givenName A. A.
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011625744037.33
122 rdf:type schema:Person
123 sg:person.01307633263.55 schema:affiliation https://www.grid.ac/institutes/grid.448543.a
124 schema:familyName Agca
125 schema:givenName C. A.
126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307633263.55
127 rdf:type schema:Person
128 sg:person.0760776064.95 schema:affiliation https://www.grid.ac/institutes/grid.467929.7
129 schema:familyName Baydas
130 schema:givenName G.
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760776064.95
132 rdf:type schema:Person
133 sg:pub.10.1007/s11062-016-9568-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010336368
134 https://doi.org/10.1007/s11062-016-9568-8
135 rdf:type schema:CreativeWork
136 sg:pub.10.1007/s11064-016-1964-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024280031
137 https://doi.org/10.1007/s11064-016-1964-3
138 rdf:type schema:CreativeWork
139 sg:pub.10.1038/celldisc.2017.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083885240
140 https://doi.org/10.1038/celldisc.2017.1
141 rdf:type schema:CreativeWork
142 sg:pub.10.1038/nrc3748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038014944
143 https://doi.org/10.1038/nrc3748
144 rdf:type schema:CreativeWork
145 sg:pub.10.1038/nrm3376 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025478850
146 https://doi.org/10.1038/nrm3376
147 rdf:type schema:CreativeWork
148 sg:pub.10.1038/nrn1824 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045889344
149 https://doi.org/10.1038/nrn1824
150 rdf:type schema:CreativeWork
151 sg:pub.10.1038/onc.2015.210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025993546
152 https://doi.org/10.1038/onc.2015.210
153 rdf:type schema:CreativeWork
154 sg:pub.10.1046/j.1440-1711.1999.00823.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031731769
155 https://doi.org/10.1046/j.1440-1711.1999.00823.x
156 rdf:type schema:CreativeWork
157 sg:pub.10.1186/1472-6882-9-45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009920114
158 https://doi.org/10.1186/1472-6882-9-45
159 rdf:type schema:CreativeWork
160 sg:pub.10.1186/1478-811x-8-31 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017427406
161 https://doi.org/10.1186/1478-811x-8-31
162 rdf:type schema:CreativeWork
163 sg:pub.10.1186/s12906-015-0677-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039408033
164 https://doi.org/10.1186/s12906-015-0677-5
165 rdf:type schema:CreativeWork
166 sg:pub.10.1186/s40478-016-0350-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021297844
167 https://doi.org/10.1186/s40478-016-0350-3
168 rdf:type schema:CreativeWork
169 https://app.dimensions.ai/details/publication/pub.1079959685 schema:CreativeWork
170 https://doi.org/10.1002/jcb.20480 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003795584
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1002/ptr.5605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002803433
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/j.biopha.2016.06.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021361511
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.biopha.2017.06.072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090346772
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.canlet.2006.01.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026964533
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/j.cub.2014.03.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017299042
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/j.fct.2010.09.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029865911
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/j.jep.2005.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001975493
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.neuron.2013.12.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051681631
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.phymed.2014.04.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044568427
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.tibs.2009.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019867611
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/s0065-230x(00)79001-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038853826
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/s0308-8146(03)00216-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052180548
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1093/ecam/neh078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030490300
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1093/hmg/ddt036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016159394
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1101/cshperspect.a001651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037119425
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1111/bpa.12537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091208114
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1111/j.1742-4658.2009.07177.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022786421
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1111/j.2042-7158.2011.01331.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013086776
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1111/jnc.12611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015442679
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1111/jnc.13423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003561894
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1111/jphp.12628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025543298
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1111/jphp.12789 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090995345
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1155/2012/176287 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034008169
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1155/2013/964149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037590658
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1155/2014/145342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042182504
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1155/2014/897361 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031256128
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1371/journal.pone.0165588 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034106673
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1515/znc-2001-7-828 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067583662
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1523/jneurosci.23-10-04034.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075290601
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1523/jneurosci.5552-09.2010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047332867
233 rdf:type schema:CreativeWork
234 https://doi.org/10.15407/ubj89.02.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085268717
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1590/s1517-83822013005000062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037879694
237 rdf:type schema:CreativeWork
238 https://doi.org/10.18097/pbmc20156101041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1068571412
239 rdf:type schema:CreativeWork
240 https://doi.org/10.2174/1567205011310030004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069188049
241 rdf:type schema:CreativeWork
242 https://doi.org/10.2174/157015911795596540 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024580607
243 rdf:type schema:CreativeWork
244 https://doi.org/10.2478/v10039-012-0042-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070852163
245 rdf:type schema:CreativeWork
246 https://doi.org/10.3390/molecules191219610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024394457
247 rdf:type schema:CreativeWork
248 https://doi.org/10.3892/etm.2016.3070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071503995
249 rdf:type schema:CreativeWork
250 https://doi.org/10.3892/mmr.2016.4948 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008922060
251 rdf:type schema:CreativeWork
252 https://www.grid.ac/institutes/grid.419966.5 schema:alternateName Palladin Institute of Biochemistry
253 schema:name Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv, Ukraine
254 rdf:type schema:Organization
255 https://www.grid.ac/institutes/grid.448543.a schema:alternateName Bingöl University
256 schema:name Bingöl University, Bingöl, Turkey
257 Oles’ Honchar Dnipro National University, Dnipro, Ukraine
258 rdf:type schema:Organization
259 https://www.grid.ac/institutes/grid.467929.7 schema:alternateName Council of Higher Education
260 schema:name Council of Higher Education, Ankara, Turkey
261 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...