Effects of Levetiracetam on Aggregation and Fusion of Membranes of Synaptic Vesicles in a Cell-Free Exocytosis Model View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-04

AUTHORS

V. P. Gumenyuk, I. O. Trikash

ABSTRACT

We studied the effects of an antiepileptic drug, levetiracetam, on the processes of aggregation of synaptic vesicles (SVs) and their calcium-induced fusion with the target membranes in a cell-free model of neurosecretion. The size of SVs and their aggregates in the suspension was measured using laser-correlation spectroscopy. Fusion of the membrane structures was estimated quantitatively according to changes in the intensity of fluorescence self-quenching of the R18 probe (octadecylrhodamine B chloride). The action of levetiracetam resulted in intensification of clustering of SVs and decrease in the level of calcium-stimulated fusion of such vesicles both with the plasma membranes and with each other. Therefore, we obtained novel data on the effect of levetiracetam on regulation of the exocytosis process at stages of SV clustering and calcium-stimulated fusion of SVs with the target membranes. More... »

PAGES

115-119

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11062-014-9416-7

DOI

http://dx.doi.org/10.1007/s11062-014-9416-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035786485


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1116", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Physiology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gumenyuk", 
        "givenName": "V. P.", 
        "id": "sg:person.01137345775.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137345775.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.419966.5", 
          "name": [
            "Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Trikash", 
        "givenName": "I. O.", 
        "id": "sg:person.060040255.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.060040255.77"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s11062-005-0014-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022246737", 
          "https://doi.org/10.1007/s11062-005-0014-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11062-010-9118-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045012887", 
          "https://doi.org/10.1007/s11062-010-9118-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/362318a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047914698", 
          "https://doi.org/10.1038/362318a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-04", 
    "datePublishedReg": "2014-04-01", 
    "description": "We studied the effects of an antiepileptic drug, levetiracetam, on the processes of aggregation of synaptic vesicles (SVs) and their calcium-induced fusion with the target membranes in a cell-free model of neurosecretion. The size of SVs and their aggregates in the suspension was measured using laser-correlation spectroscopy. Fusion of the membrane structures was estimated quantitatively according to changes in the intensity of fluorescence self-quenching of the R18 probe (octadecylrhodamine B chloride). The action of levetiracetam resulted in intensification of clustering of SVs and decrease in the level of calcium-stimulated fusion of such vesicles both with the plasma membranes and with each other. Therefore, we obtained novel data on the effect of levetiracetam on regulation of the exocytosis process at stages of SV clustering and calcium-stimulated fusion of SVs with the target membranes.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s11062-014-9416-7", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1016854", 
        "issn": [
          "0090-2977", 
          "1573-9007"
        ], 
        "name": "Neurophysiology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "46"
      }
    ], 
    "keywords": [
      "synaptic vesicles", 
      "target membrane", 
      "size of SVs", 
      "fusion of membranes", 
      "plasma membrane", 
      "exocytosis process", 
      "calcium-induced fusion", 
      "such vesicles", 
      "membrane structure", 
      "action of levetiracetam", 
      "vesicles", 
      "cell-free model", 
      "process of aggregation", 
      "membrane", 
      "exocytosis model", 
      "intensity of fluorescence", 
      "fusion", 
      "novel data", 
      "regulation", 
      "aggregation", 
      "neurosecretion", 
      "fluorescence", 
      "probe", 
      "aggregates", 
      "stage", 
      "process", 
      "effect", 
      "levels", 
      "clustering", 
      "action", 
      "intensification", 
      "structure", 
      "changes", 
      "size", 
      "decrease", 
      "suspension", 
      "data", 
      "model", 
      "drugs", 
      "intensity", 
      "effect of levetiracetam", 
      "spectroscopy", 
      "antiepileptic drugs", 
      "levetiracetam", 
      "laser-correlation spectroscopy", 
      "R18 probe", 
      "intensification of clustering", 
      "calcium-stimulated fusion", 
      "stages of SV", 
      "Cell-Free Exocytosis Model"
    ], 
    "name": "Effects of Levetiracetam on Aggregation and Fusion of Membranes of Synaptic Vesicles in a Cell-Free Exocytosis Model", 
    "pagination": "115-119", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1035786485"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11062-014-9416-7"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11062-014-9416-7", 
      "https://app.dimensions.ai/details/publication/pub.1035786485"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:31", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_633.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s11062-014-9416-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11062-014-9416-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11062-014-9416-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11062-014-9416-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11062-014-9416-7'


 

This table displays all metadata directly associated to this object as RDF triples.

131 TRIPLES      22 PREDICATES      80 URIs      68 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11062-014-9416-7 schema:about anzsrc-for:11
2 anzsrc-for:1109
3 anzsrc-for:1116
4 schema:author N5212aa3945b947f9844f488feae309e3
5 schema:citation sg:pub.10.1007/s11062-005-0014-6
6 sg:pub.10.1007/s11062-010-9118-8
7 sg:pub.10.1038/362318a0
8 schema:datePublished 2014-04
9 schema:datePublishedReg 2014-04-01
10 schema:description We studied the effects of an antiepileptic drug, levetiracetam, on the processes of aggregation of synaptic vesicles (SVs) and their calcium-induced fusion with the target membranes in a cell-free model of neurosecretion. The size of SVs and their aggregates in the suspension was measured using laser-correlation spectroscopy. Fusion of the membrane structures was estimated quantitatively according to changes in the intensity of fluorescence self-quenching of the R18 probe (octadecylrhodamine B chloride). The action of levetiracetam resulted in intensification of clustering of SVs and decrease in the level of calcium-stimulated fusion of such vesicles both with the plasma membranes and with each other. Therefore, we obtained novel data on the effect of levetiracetam on regulation of the exocytosis process at stages of SV clustering and calcium-stimulated fusion of SVs with the target membranes.
11 schema:genre article
12 schema:inLanguage en
13 schema:isAccessibleForFree false
14 schema:isPartOf N0deee07de92347fd95bff3780ba99347
15 N46b499592c6f48978d94b3cec580f75e
16 sg:journal.1016854
17 schema:keywords Cell-Free Exocytosis Model
18 R18 probe
19 action
20 action of levetiracetam
21 aggregates
22 aggregation
23 antiepileptic drugs
24 calcium-induced fusion
25 calcium-stimulated fusion
26 cell-free model
27 changes
28 clustering
29 data
30 decrease
31 drugs
32 effect
33 effect of levetiracetam
34 exocytosis model
35 exocytosis process
36 fluorescence
37 fusion
38 fusion of membranes
39 intensification
40 intensification of clustering
41 intensity
42 intensity of fluorescence
43 laser-correlation spectroscopy
44 levels
45 levetiracetam
46 membrane
47 membrane structure
48 model
49 neurosecretion
50 novel data
51 plasma membrane
52 probe
53 process
54 process of aggregation
55 regulation
56 size
57 size of SVs
58 spectroscopy
59 stage
60 stages of SV
61 structure
62 such vesicles
63 suspension
64 synaptic vesicles
65 target membrane
66 vesicles
67 schema:name Effects of Levetiracetam on Aggregation and Fusion of Membranes of Synaptic Vesicles in a Cell-Free Exocytosis Model
68 schema:pagination 115-119
69 schema:productId N64f558e54c17462894d89ee4a0b02b0c
70 Nfcdabc53f11d4dee83baec6c47b1123e
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035786485
72 https://doi.org/10.1007/s11062-014-9416-7
73 schema:sdDatePublished 2021-12-01T19:31
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher Nde2c3f7d69594231b26fe6a98cc1ca0e
76 schema:url https://doi.org/10.1007/s11062-014-9416-7
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N0deee07de92347fd95bff3780ba99347 schema:issueNumber 2
81 rdf:type schema:PublicationIssue
82 N2de50044087b400b84c704f8c53f59f5 rdf:first sg:person.060040255.77
83 rdf:rest rdf:nil
84 N46b499592c6f48978d94b3cec580f75e schema:volumeNumber 46
85 rdf:type schema:PublicationVolume
86 N5212aa3945b947f9844f488feae309e3 rdf:first sg:person.01137345775.17
87 rdf:rest N2de50044087b400b84c704f8c53f59f5
88 N64f558e54c17462894d89ee4a0b02b0c schema:name dimensions_id
89 schema:value pub.1035786485
90 rdf:type schema:PropertyValue
91 Nde2c3f7d69594231b26fe6a98cc1ca0e schema:name Springer Nature - SN SciGraph project
92 rdf:type schema:Organization
93 Nfcdabc53f11d4dee83baec6c47b1123e schema:name doi
94 schema:value 10.1007/s11062-014-9416-7
95 rdf:type schema:PropertyValue
96 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
97 schema:name Medical and Health Sciences
98 rdf:type schema:DefinedTerm
99 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
100 schema:name Neurosciences
101 rdf:type schema:DefinedTerm
102 anzsrc-for:1116 schema:inDefinedTermSet anzsrc-for:
103 schema:name Medical Physiology
104 rdf:type schema:DefinedTerm
105 sg:journal.1016854 schema:issn 0090-2977
106 1573-9007
107 schema:name Neurophysiology
108 schema:publisher Springer Nature
109 rdf:type schema:Periodical
110 sg:person.01137345775.17 schema:affiliation grid-institutes:grid.419966.5
111 schema:familyName Gumenyuk
112 schema:givenName V. P.
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137345775.17
114 rdf:type schema:Person
115 sg:person.060040255.77 schema:affiliation grid-institutes:grid.419966.5
116 schema:familyName Trikash
117 schema:givenName I. O.
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.060040255.77
119 rdf:type schema:Person
120 sg:pub.10.1007/s11062-005-0014-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022246737
121 https://doi.org/10.1007/s11062-005-0014-6
122 rdf:type schema:CreativeWork
123 sg:pub.10.1007/s11062-010-9118-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045012887
124 https://doi.org/10.1007/s11062-010-9118-8
125 rdf:type schema:CreativeWork
126 sg:pub.10.1038/362318a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047914698
127 https://doi.org/10.1038/362318a0
128 rdf:type schema:CreativeWork
129 grid-institutes:grid.419966.5 schema:alternateName Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
130 schema:name Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine
131 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...