Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-01

AUTHORS

Camilla B. Mitchell, Bronte Black, Faith Sun, Wojciech Chrzanowski, Justin Cooper-White, Benois Maisonneuve, Brett Stringer, Bryan Day, Maté Biro, Geraldine M. O’Neill

ABSTRACT

INTRODUCTION: The brain is a very soft tissue. Glioblastoma (GBM) brain tumours are highly infiltrative into the surrounding healthy brain tissue and invasion mechanisms that have been defined using rigid substrates therefore may not apply to GBM dissemination. GBMs characteristically lose expression of the high molecular weight tropomyosins, a class of actin-associating proteins and essential regulators of the actin stress fibres and focal adhesions that underpin cell migration on rigid substrates. METHODS: Here, we investigated how loss of the high molecular weight tropomyosins affects GBM on soft matrices that recapitulate the biomechanical architecture of the brain. RESULTS: We find that Tpm 2.1 is down-regulated in GBM grown on soft substrates. We demonstrate that Tpm 2.1 depletion by siRNA induces cell spreading and elongation in soft 3D hydrogels, irrespective of matrix composition. Tpm 1.7, a second high molecular weight tropomyosin is also down-regulated when cells are cultured on soft brain-like surfaces and we show that effects of this isoform are matrix dependent, with Tpm 1.7 inducing cell rounding in 3D collagen gels. Finally, we show that the absence of Tpm 2.1 from primary patient-derived GBMs correlates with elongated, mesenchymal invasion. CONCLUSIONS: We propose that Tpm 2.1 down-regulation facilitates GBM colonisation of the soft brain environment. This specialisation of the GBM actin cytoskeleton organisation that is highly suited to the soft brain-like environment may provide novel therapeutic targets for arresting GBM invasion. More... »

PAGES

1-11

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11060-018-03049-z

DOI

http://dx.doi.org/10.1007/s11060-018-03049-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110487099

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30535593


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Peter MacCallum Cancer Centre", 
          "id": "https://www.grid.ac/institutes/grid.1055.1", 
          "name": [
            "Children\u2019s Cancer Research Unit, Kids Research Institute, The Children\u2019s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia", 
            "Division of Cancer Research, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mitchell", 
        "givenName": "Camilla B.", 
        "id": "sg:person.016420340705.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016420340705.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Children's Hospital at Westmead", 
          "id": "https://www.grid.ac/institutes/grid.413973.b", 
          "name": [
            "Children\u2019s Cancer Research Unit, Kids Research Institute, The Children\u2019s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Black", 
        "givenName": "Bronte", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Children's Hospital at Westmead", 
          "id": "https://www.grid.ac/institutes/grid.413973.b", 
          "name": [
            "Children\u2019s Cancer Research Unit, Kids Research Institute, The Children\u2019s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sun", 
        "givenName": "Faith", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sydney", 
          "id": "https://www.grid.ac/institutes/grid.1013.3", 
          "name": [
            "Faculty of Pharmacy, The University of Sydney, 2006, Sydney, NSW, Australia", 
            "The University of Sydney Nano Institute, 2006, Sydney, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chrzanowski", 
        "givenName": "Wojciech", 
        "id": "sg:person.0762577330.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762577330.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Queensland", 
          "id": "https://www.grid.ac/institutes/grid.1003.2", 
          "name": [
            "Tissue Engineering and Microfluidics Group, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, Brisbane, Australia", 
            "School of Chemical Engineering, The University of Queensland, 4072, Brisbane, Australia", 
            "CSIRO, Manufacturing Flagship, Biomedical Manufacturing Program, Clayton, VIC, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cooper-White", 
        "givenName": "Justin", 
        "id": "sg:person.01323561015.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323561015.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "CSIRO, Manufacturing Flagship, Biomedical Manufacturing Program, Clayton, VIC, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maisonneuve", 
        "givenName": "Benois", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "QIMR Berghofer Medical Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.1049.c", 
          "name": [
            "QIMR Berghofer Medical Research Institute, Herston, QLD, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stringer", 
        "givenName": "Brett", 
        "id": "sg:person.0613163612.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613163612.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "QIMR Berghofer Medical Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.1049.c", 
          "name": [
            "QIMR Berghofer Medical Research Institute, Herston, QLD, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Day", 
        "givenName": "Bryan", 
        "id": "sg:person.01363431606.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363431606.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sydney", 
          "id": "https://www.grid.ac/institutes/grid.1013.3", 
          "name": [
            "EMBL Australia Single Molecule Science Node, School of Medical Sciences, The University of New South Wales, Sydney, Australia", 
            "Centenary Institute, Sydney Medical School, The University of Sydney, Sydney, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Biro", 
        "givenName": "Mat\u00e9", 
        "id": "sg:person.01215446236.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215446236.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sydney", 
          "id": "https://www.grid.ac/institutes/grid.1013.3", 
          "name": [
            "Children\u2019s Cancer Research Unit, Kids Research Institute, The Children\u2019s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia", 
            "Discipline of Childhood and Adolescent Health, The University of Sydney, Sydney, NSW, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "O\u2019Neill", 
        "givenName": "Geraldine M.", 
        "id": "sg:person.01014067274.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014067274.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1529/biophysj.105.061994", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001888299"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/bioa.1.4.17897", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002411526"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.06.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002736945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.12688/f1000research.8800.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003820991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.12688/f1000research.8800.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003820991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/onc.2010.516", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004205372", 
          "https://doi.org/10.1038/onc.2010.516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01050417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005242822", 
          "https://doi.org/10.1007/bf01050417"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep23353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008806829", 
          "https://doi.org/10.1038/srep23353"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.1349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010130279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/cancers5020357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013948245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200209006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014602760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2016.03.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016974430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/0471143030.cb1016s47", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021547547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-12-4501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021976028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2009.05.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023870544"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2011.03.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027560502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.079145", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027644597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.153.4.881", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028398903"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-13-3426", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028588796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/19336918.2016.1183868", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031750686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6601201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035313899", 
          "https://doi.org/10.1038/sj.bjc.6601201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6601201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035313899", 
          "https://doi.org/10.1038/sj.bjc.6601201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ejcb.2005.11.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038149142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2005.08.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038845984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccr.2005.08.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038845984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb3277", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039841569", 
          "https://doi.org/10.1038/ncb3277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-4827(03)00256-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041402074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-4827(03)00256-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041402074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2012.0189", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041849079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-08-4859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043606962"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.106039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044820809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/glia.10174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047674055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2005.10.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048566204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2005.10.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048566204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.10.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048663939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0604460103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049654998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2008.09.043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052443110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/1367-2630/16/7/075002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059136490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00006123-199608000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060245941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2003.05.063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064203593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/cam.3.4.9468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072291955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.192930", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083854486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1535-7163.mct-16-0873", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085471804"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/glia.23206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091875999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fncel.2018.00025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101137806"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-01", 
    "datePublishedReg": "2019-01-01", 
    "description": "INTRODUCTION: The brain is a very soft tissue. Glioblastoma (GBM) brain tumours are highly infiltrative into the surrounding healthy brain tissue and invasion mechanisms that have been defined using rigid substrates therefore may not apply to GBM dissemination. GBMs characteristically lose expression of the high molecular weight tropomyosins, a class of actin-associating proteins and essential regulators of the actin stress fibres and focal adhesions that underpin cell migration on rigid substrates.\nMETHODS: Here, we investigated how loss of the high molecular weight tropomyosins affects GBM on soft matrices that recapitulate the biomechanical architecture of the brain.\nRESULTS: We find that Tpm 2.1 is down-regulated in GBM grown on soft substrates. We demonstrate that Tpm 2.1 depletion by siRNA induces cell spreading and elongation in soft 3D hydrogels, irrespective of matrix composition. Tpm 1.7, a second high molecular weight tropomyosin is also down-regulated when cells are cultured on soft brain-like surfaces and we show that effects of this isoform are matrix dependent, with Tpm 1.7 inducing cell rounding in 3D collagen gels. Finally, we show that the absence of Tpm 2.1 from primary patient-derived GBMs correlates with elongated, mesenchymal invasion.\nCONCLUSIONS: We propose that Tpm 2.1 down-regulation facilitates GBM colonisation of the soft brain environment. This specialisation of the GBM actin cytoskeleton organisation that is highly suited to the soft brain-like environment may provide novel therapeutic targets for arresting GBM invasion.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11060-018-03049-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1094205", 
        "issn": [
          "0167-594X", 
          "1573-7373"
        ], 
        "name": "Journal of Neuro-Oncology", 
        "type": "Periodical"
      }
    ], 
    "name": "Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments", 
    "pagination": "1-11", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "862b3a648b6be7c83d69f57760decbbbb5b3a256eecf3dca3cf000c92f7f640f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30535593"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8309335"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11060-018-03049-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1110487099"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11060-018-03049-z", 
      "https://app.dimensions.ai/details/publication/pub.1110487099"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T08:32", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000309_0000000309/records_106289_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs11060-018-03049-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11060-018-03049-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11060-018-03049-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11060-018-03049-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11060-018-03049-z'


 

This table displays all metadata directly associated to this object as RDF triples.

270 TRIPLES      21 PREDICATES      67 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11060-018-03049-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N626f961c9016491c8b70e42a2a38b27e
4 schema:citation sg:pub.10.1007/bf01050417
5 sg:pub.10.1038/ncb3277
6 sg:pub.10.1038/onc.2010.516
7 sg:pub.10.1038/sj.bjc.6601201
8 sg:pub.10.1038/srep23353
9 https://doi.org/10.1002/0471143030.cb1016s47
10 https://doi.org/10.1002/glia.10174
11 https://doi.org/10.1002/glia.23206
12 https://doi.org/10.1002/stem.1349
13 https://doi.org/10.1016/j.bbamcr.2005.10.008
14 https://doi.org/10.1016/j.ccr.2005.08.010
15 https://doi.org/10.1016/j.cell.2006.06.044
16 https://doi.org/10.1016/j.cell.2008.09.043
17 https://doi.org/10.1016/j.cell.2009.10.027
18 https://doi.org/10.1016/j.cub.2011.03.007
19 https://doi.org/10.1016/j.cub.2016.03.028
20 https://doi.org/10.1016/j.ejcb.2005.11.001
21 https://doi.org/10.1016/j.jsb.2009.05.005
22 https://doi.org/10.1016/s0014-4827(03)00256-8
23 https://doi.org/10.1073/pnas.0604460103
24 https://doi.org/10.1080/19336918.2016.1183868
25 https://doi.org/10.1083/jcb.153.4.881
26 https://doi.org/10.1083/jcb.200209006
27 https://doi.org/10.1088/1367-2630/16/7/075002
28 https://doi.org/10.1089/scd.2012.0189
29 https://doi.org/10.1097/00006123-199608000-00001
30 https://doi.org/10.1158/0008-5472.can-08-4859
31 https://doi.org/10.1158/0008-5472.can-12-4501
32 https://doi.org/10.1158/0008-5472.can-13-3426
33 https://doi.org/10.1158/1535-7163.mct-16-0873
34 https://doi.org/10.1200/jco.2003.05.063
35 https://doi.org/10.1242/dev.079145
36 https://doi.org/10.1242/dev.106039
37 https://doi.org/10.1242/jcs.192930
38 https://doi.org/10.12688/f1000research.8800.1
39 https://doi.org/10.1529/biophysj.105.061994
40 https://doi.org/10.3389/fncel.2018.00025
41 https://doi.org/10.3390/cancers5020357
42 https://doi.org/10.4161/bioa.1.4.17897
43 https://doi.org/10.4161/cam.3.4.9468
44 schema:datePublished 2019-01
45 schema:datePublishedReg 2019-01-01
46 schema:description INTRODUCTION: The brain is a very soft tissue. Glioblastoma (GBM) brain tumours are highly infiltrative into the surrounding healthy brain tissue and invasion mechanisms that have been defined using rigid substrates therefore may not apply to GBM dissemination. GBMs characteristically lose expression of the high molecular weight tropomyosins, a class of actin-associating proteins and essential regulators of the actin stress fibres and focal adhesions that underpin cell migration on rigid substrates. METHODS: Here, we investigated how loss of the high molecular weight tropomyosins affects GBM on soft matrices that recapitulate the biomechanical architecture of the brain. RESULTS: We find that Tpm 2.1 is down-regulated in GBM grown on soft substrates. We demonstrate that Tpm 2.1 depletion by siRNA induces cell spreading and elongation in soft 3D hydrogels, irrespective of matrix composition. Tpm 1.7, a second high molecular weight tropomyosin is also down-regulated when cells are cultured on soft brain-like surfaces and we show that effects of this isoform are matrix dependent, with Tpm 1.7 inducing cell rounding in 3D collagen gels. Finally, we show that the absence of Tpm 2.1 from primary patient-derived GBMs correlates with elongated, mesenchymal invasion. CONCLUSIONS: We propose that Tpm 2.1 down-regulation facilitates GBM colonisation of the soft brain environment. This specialisation of the GBM actin cytoskeleton organisation that is highly suited to the soft brain-like environment may provide novel therapeutic targets for arresting GBM invasion.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree false
50 schema:isPartOf sg:journal.1094205
51 schema:name Tropomyosin Tpm 2.1 loss induces glioblastoma spreading in soft brain-like environments
52 schema:pagination 1-11
53 schema:productId N19b3e5a202f34093ab15778d78a202e9
54 N229c24f846b346789c0a125bfa94d89d
55 N44908a08da3a4eafacd0e9a614f6ef36
56 N6e297433bc904b64a7ed72c5af0f62f1
57 N7cab50486026434c866b553ded6d41c8
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110487099
59 https://doi.org/10.1007/s11060-018-03049-z
60 schema:sdDatePublished 2019-04-11T08:32
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher N48ebc9ec6bb848e384feef5c0ed4d72c
63 schema:url https://link.springer.com/10.1007%2Fs11060-018-03049-z
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N168ee82dbb0a4fe1b6ef33249f04bc55 rdf:first N4d2d7f9dba5a491eb762c09e33a81572
68 rdf:rest Nc3971b6277ef4d3bbd35a4653d1cc2e6
69 N19b3e5a202f34093ab15778d78a202e9 schema:name dimensions_id
70 schema:value pub.1110487099
71 rdf:type schema:PropertyValue
72 N1bc8a428866949068f12a02828e4d7c4 schema:affiliation https://www.grid.ac/institutes/grid.413973.b
73 schema:familyName Sun
74 schema:givenName Faith
75 rdf:type schema:Person
76 N2049eaeb4c394129b905f1356eeb35f3 rdf:first Nfe86042ce5bf47b39a4632d4332107cf
77 rdf:rest N2a51f7c0e8dc4f9fbd0f0a2e86432604
78 N229c24f846b346789c0a125bfa94d89d schema:name readcube_id
79 schema:value 862b3a648b6be7c83d69f57760decbbbb5b3a256eecf3dca3cf000c92f7f640f
80 rdf:type schema:PropertyValue
81 N2a51f7c0e8dc4f9fbd0f0a2e86432604 rdf:first sg:person.0613163612.14
82 rdf:rest Nafac1da09e444cd093f6779238a11c09
83 N44908a08da3a4eafacd0e9a614f6ef36 schema:name doi
84 schema:value 10.1007/s11060-018-03049-z
85 rdf:type schema:PropertyValue
86 N48ebc9ec6bb848e384feef5c0ed4d72c schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N4d2d7f9dba5a491eb762c09e33a81572 schema:affiliation https://www.grid.ac/institutes/grid.413973.b
89 schema:familyName Black
90 schema:givenName Bronte
91 rdf:type schema:Person
92 N626f961c9016491c8b70e42a2a38b27e rdf:first sg:person.016420340705.00
93 rdf:rest N168ee82dbb0a4fe1b6ef33249f04bc55
94 N6e297433bc904b64a7ed72c5af0f62f1 schema:name nlm_unique_id
95 schema:value 8309335
96 rdf:type schema:PropertyValue
97 N77cc6ae463de413b919eb3a1b232d460 rdf:first sg:person.0762577330.44
98 rdf:rest N9b69fbd7a939435496b46f1ffd4037df
99 N7cab50486026434c866b553ded6d41c8 schema:name pubmed_id
100 schema:value 30535593
101 rdf:type schema:PropertyValue
102 N9b69fbd7a939435496b46f1ffd4037df rdf:first sg:person.01323561015.28
103 rdf:rest N2049eaeb4c394129b905f1356eeb35f3
104 Nafac1da09e444cd093f6779238a11c09 rdf:first sg:person.01363431606.16
105 rdf:rest Nbe07ba5cb19e4e44ba857eddadbf25a4
106 Nbe07ba5cb19e4e44ba857eddadbf25a4 rdf:first sg:person.01215446236.20
107 rdf:rest Ncae001be17334568858dfc7696214b3c
108 Nc3971b6277ef4d3bbd35a4653d1cc2e6 rdf:first N1bc8a428866949068f12a02828e4d7c4
109 rdf:rest N77cc6ae463de413b919eb3a1b232d460
110 Ncae001be17334568858dfc7696214b3c rdf:first sg:person.01014067274.14
111 rdf:rest rdf:nil
112 Nf08476359f3245afa9bf5ddf5c59b6b5 schema:name CSIRO, Manufacturing Flagship, Biomedical Manufacturing Program, Clayton, VIC, Australia
113 rdf:type schema:Organization
114 Nfe86042ce5bf47b39a4632d4332107cf schema:affiliation Nf08476359f3245afa9bf5ddf5c59b6b5
115 schema:familyName Maisonneuve
116 schema:givenName Benois
117 rdf:type schema:Person
118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biological Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biochemistry and Cell Biology
123 rdf:type schema:DefinedTerm
124 sg:journal.1094205 schema:issn 0167-594X
125 1573-7373
126 schema:name Journal of Neuro-Oncology
127 rdf:type schema:Periodical
128 sg:person.01014067274.14 schema:affiliation https://www.grid.ac/institutes/grid.1013.3
129 schema:familyName O’Neill
130 schema:givenName Geraldine M.
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014067274.14
132 rdf:type schema:Person
133 sg:person.01215446236.20 schema:affiliation https://www.grid.ac/institutes/grid.1013.3
134 schema:familyName Biro
135 schema:givenName Maté
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215446236.20
137 rdf:type schema:Person
138 sg:person.01323561015.28 schema:affiliation https://www.grid.ac/institutes/grid.1003.2
139 schema:familyName Cooper-White
140 schema:givenName Justin
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323561015.28
142 rdf:type schema:Person
143 sg:person.01363431606.16 schema:affiliation https://www.grid.ac/institutes/grid.1049.c
144 schema:familyName Day
145 schema:givenName Bryan
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363431606.16
147 rdf:type schema:Person
148 sg:person.016420340705.00 schema:affiliation https://www.grid.ac/institutes/grid.1055.1
149 schema:familyName Mitchell
150 schema:givenName Camilla B.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016420340705.00
152 rdf:type schema:Person
153 sg:person.0613163612.14 schema:affiliation https://www.grid.ac/institutes/grid.1049.c
154 schema:familyName Stringer
155 schema:givenName Brett
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613163612.14
157 rdf:type schema:Person
158 sg:person.0762577330.44 schema:affiliation https://www.grid.ac/institutes/grid.1013.3
159 schema:familyName Chrzanowski
160 schema:givenName Wojciech
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762577330.44
162 rdf:type schema:Person
163 sg:pub.10.1007/bf01050417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005242822
164 https://doi.org/10.1007/bf01050417
165 rdf:type schema:CreativeWork
166 sg:pub.10.1038/ncb3277 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039841569
167 https://doi.org/10.1038/ncb3277
168 rdf:type schema:CreativeWork
169 sg:pub.10.1038/onc.2010.516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004205372
170 https://doi.org/10.1038/onc.2010.516
171 rdf:type schema:CreativeWork
172 sg:pub.10.1038/sj.bjc.6601201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035313899
173 https://doi.org/10.1038/sj.bjc.6601201
174 rdf:type schema:CreativeWork
175 sg:pub.10.1038/srep23353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008806829
176 https://doi.org/10.1038/srep23353
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1002/0471143030.cb1016s47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021547547
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1002/glia.10174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047674055
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1002/glia.23206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091875999
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1002/stem.1349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010130279
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/j.bbamcr.2005.10.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048566204
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.ccr.2005.08.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038845984
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.cell.2006.06.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002736945
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.cell.2008.09.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052443110
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.cell.2009.10.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048663939
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.cub.2011.03.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027560502
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.cub.2016.03.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016974430
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.ejcb.2005.11.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038149142
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.jsb.2009.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023870544
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/s0014-4827(03)00256-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041402074
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1073/pnas.0604460103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049654998
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1080/19336918.2016.1183868 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031750686
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1083/jcb.153.4.881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028398903
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1083/jcb.200209006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014602760
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1088/1367-2630/16/7/075002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059136490
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1089/scd.2012.0189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041849079
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1097/00006123-199608000-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060245941
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1158/0008-5472.can-08-4859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043606962
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1158/0008-5472.can-12-4501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021976028
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1158/0008-5472.can-13-3426 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028588796
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1158/1535-7163.mct-16-0873 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085471804
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1200/jco.2003.05.063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064203593
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1242/dev.079145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027644597
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1242/dev.106039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044820809
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1242/jcs.192930 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083854486
235 rdf:type schema:CreativeWork
236 https://doi.org/10.12688/f1000research.8800.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003820991
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1529/biophysj.105.061994 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001888299
239 rdf:type schema:CreativeWork
240 https://doi.org/10.3389/fncel.2018.00025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101137806
241 rdf:type schema:CreativeWork
242 https://doi.org/10.3390/cancers5020357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013948245
243 rdf:type schema:CreativeWork
244 https://doi.org/10.4161/bioa.1.4.17897 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002411526
245 rdf:type schema:CreativeWork
246 https://doi.org/10.4161/cam.3.4.9468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072291955
247 rdf:type schema:CreativeWork
248 https://www.grid.ac/institutes/grid.1003.2 schema:alternateName University of Queensland
249 schema:name CSIRO, Manufacturing Flagship, Biomedical Manufacturing Program, Clayton, VIC, Australia
250 School of Chemical Engineering, The University of Queensland, 4072, Brisbane, Australia
251 Tissue Engineering and Microfluidics Group, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, 4072, Brisbane, Australia
252 rdf:type schema:Organization
253 https://www.grid.ac/institutes/grid.1013.3 schema:alternateName University of Sydney
254 schema:name Centenary Institute, Sydney Medical School, The University of Sydney, Sydney, Australia
255 Children’s Cancer Research Unit, Kids Research Institute, The Children’s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia
256 Discipline of Childhood and Adolescent Health, The University of Sydney, Sydney, NSW, Australia
257 EMBL Australia Single Molecule Science Node, School of Medical Sciences, The University of New South Wales, Sydney, Australia
258 Faculty of Pharmacy, The University of Sydney, 2006, Sydney, NSW, Australia
259 The University of Sydney Nano Institute, 2006, Sydney, NSW, Australia
260 rdf:type schema:Organization
261 https://www.grid.ac/institutes/grid.1049.c schema:alternateName QIMR Berghofer Medical Research Institute
262 schema:name QIMR Berghofer Medical Research Institute, Herston, QLD, Australia
263 rdf:type schema:Organization
264 https://www.grid.ac/institutes/grid.1055.1 schema:alternateName Peter MacCallum Cancer Centre
265 schema:name Children’s Cancer Research Unit, Kids Research Institute, The Children’s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia
266 Division of Cancer Research, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia
267 rdf:type schema:Organization
268 https://www.grid.ac/institutes/grid.413973.b schema:alternateName Children's Hospital at Westmead
269 schema:name Children’s Cancer Research Unit, Kids Research Institute, The Children’s Hospital at Westmead, Locked Bag 4001, 2145, Westmead, NSW, Australia
270 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...