The detection of HBV DNA with gold-coated iron oxide nanoparticle gene probes View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2007-07-17

AUTHORS

Dong Xi, XiaoPing Luo, QiangHua Lu, KaiLun Yao, ZuLi Liu, Qin Ning

ABSTRACT

Gold-coated iron oxide nanoparticle Hepatitis B virus (HBV) DNA probes were prepared, and their application for HBV DNA measurement was studied. Gold-coated iron oxide nanoparticles were prepared by the citrate reduction of tetra-chloroauric acid in the presence of iron oxide nanoparticles which were added as seeds. With a fluorescence-based method, the maximal surface coverage of hexaethiol 30-mer oligonucleotides and the maximal percentage of hybridization strands on gold-coated iron oxide nanoparticles were (120 ± 8) oligonucleotides per nanoparticle, and (14 ± 2%), respectively, which were comparable with those of (132 ± 10) and (22 ± 3%) in Au nanoparticle groups. Large network aggregates were formed when gold-coated iron oxide nanoparticle HBV DNA gene probe was applied to detect HBV DNA molecules as evidenced by transmission electron microscopy and the high specificity was verified by blot hybridization. Our results further suggested that detecting DNA with iron oxide nanoparticles and magnetic separator was feasible and might be an alternative effective method. More... »

PAGES

393-400

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11051-007-9263-1

DOI

http://dx.doi.org/10.1007/s11051-007-9263-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024668312

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/32214883


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