Regulation of vtg and VtgR in mud crab Scylla paramamosain by miR-34 View Full Text


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Article Info

DATE

2022-06-17

AUTHORS

Yinzhen Sheng, Jiaqian Liao, Ziping Zhang, Yuting Li, Xiwei Jia, Xianyuan Zeng, Yilei Wang

ABSTRACT

BackgroundVitellogenin (Vtg) is the precursor of major yolk protein and plays a crucial role in the maturation of oocytes and the production of eggs in oviparous animals. Vitellogenin receptor (VtgR) mediates the transport of Vtg explicitly to oocytes in the membrane. In a previous study, we found that miR-34 can regulate the expression of some eyestalk genes and affect reproduction in mud crab Scylla paramamosain, one of the most important economic crabs on the coasts of southern China.Methods and resultsIn this study, firstly, we found that miR-34 can target at 3’-UTR of Vtg and VtgR genes by using bioinformatic tools and predicted miR-34 might depress the expression of Vtg and VtgR. Secondly, the relative luciferase activity of HEK293T cells co-transfected with miRNA mimic and pmir-RB-REPORTTM-Vtg/VtgR-3’UTR was significantly lower than those of cells co-transfected with mimic NC and pmir-RB-REPORTTM-Vtg/VtgR-3’UTR. Finally, in vivo experiments showed that agomiR-34 could repress the expression of Vtg and VtgR genes, while Antigomir-34 could promote the expression of these two genes.ConclusionsThese results confirm our hypothesis and previous published results that miR-34 may indirectly regulate ovarian development by binding to the 3’-UTR of Vtg and VtgR genes and inhibiting their expression. More... »

PAGES

7367-7376

References to SciGraph publications

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  • 2013-09-05. Multiple vitellogenins and product yolk proteins in striped bass, Morone saxatilis: molecular characterization and processing during oocyte growth and maturation in FISH PHYSIOLOGY AND BIOCHEMISTRY
  • 2014-07-01. Global analysis of the ovarian microRNA transcriptome: implication for miR-2 and miR-133 regulation of oocyte meiosis in the Chinese mitten crab, Eriocheir sinensis (Crustacea:Decapoda) in BMC GENOMICS
  • 2003-03. Regulated portals of entry into the cell in NATURE
  • 2020-07-11. miR-9 and miR-263 Regulate the Key Genes of the ERK Pathway in the Ovary of Mud Crab Scylla paramamosain in MARINE BIOTECHNOLOGY
  • 2017-05-22. MicroRNA-275 and its target Vitellogenin-2 are crucial in ovary development and blood digestion of Haemaphysalis longicornis in PARASITES & VECTORS
  • 2017-08-28. Analysis of the miR-34 family functions in breast cancer reveals annotation error of miR-34b in SCIENTIFIC REPORTS
  • 2019-10-25. Localized expression and inhibition effect of miR-184 on blood digestion and oviposition in Haemaphysalis longicornis (Acari: Ixodidae) in PARASITES & VECTORS
  • 2010-11-16. Mechanisms and control of vitellogenesis in crustaceans in FISHERIES SCIENCE
  • 1997-09. Internalization and recycling of vitellogenin receptor in the mosquito oocyte in CELL AND TISSUE RESEARCH
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    http://scigraph.springernature.com/pub.10.1007/s11033-022-07530-x

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    http://dx.doi.org/10.1007/s11033-022-07530-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1148761080

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/35715603


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