Implantation associated divergence in neutrophil glucocorticoid and cysteine-X-cysteine receptor genes in cattle View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-06-04

AUTHORS

P. Manjari, Iqbal Hyder, A. K. Dang

ABSTRACT

The differential roles of Interferon-tau (IFNT) and Progesterone (P4) in triggering pro- and anti-inflammatory responses during peri-implantation period were assessed in cows. An ex vivo study was done by isolating neutrophils from the animals post artificial insemination (AI) at defined intervals and analyzing the expression changes of Glucocorticoid receptor-α (GRα) and Interleukin-8 receptor (CXCR1) genes. An in vitro study was also done isolating neutrophils from prepubertal heifers subjecting them to IFNT and Progesterone P4 supplementation separately. We observed that from day 14 post Artificial Insemination, there is a significant up-regulation of neutrophil GRα and CXCR1 in pregnant and non-pregnant cows respectively. The in vitro study showed that IFNT significantly up-regulates GRα whereas P4 significantly up-regulates CXCR1. The total leukocyte count and neutrophil count were also significantly higher in non-pregnant cows. We conclude that IFNT efficiently mediates neutrophil immunosuppression during peri-implantation period in cows by interacting with Interleukin 8 receptor and can also exert ligand independent actions on GRα.Graphical abstract More... »

PAGES

5493-5499

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11033-019-04865-w

DOI

http://dx.doi.org/10.1007/s11033-019-04865-w

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1116645123

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31165367


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