Ontology type: schema:ScholarlyArticle
2019-01-29
AUTHORSStefan Wagner, Judi McCracken, Sabine Bruszies, Ric Broadhurst, David N. Wells, Björn Oback, Jürgen Bode, Götz Laible
ABSTRACTEpisomal plasmids based on a scaffold/matrix attachment region (S/MAR) are extrachromosomal DNA entities that replicate once per cell cycle and are stably maintained in cells or tissue. We generated minicircles, episomal plasmids devoid of bacterial sequences, and show that they are stably transmitted in clonal primary bovine fibroblasts without selection pressure over more than two months. Total DNA, plasmid extraction and fluorescence in situ hybridization (FISH) analyses suggest that the minicircles remained episomal and were not integrated into the genome. Minicircles survived extended periods in serum-starved cells, which indicates that ongoing transcription in non-proliferating cells is not necessary for the maintenance of S/MAR-episomes. To test whether minicircles endure the process of somatic cell nuclear transfer (SCNT), we used cell-cycle synchronized, serum-starved, minicircle-containing cells. Analysis of cells outgrown from SCNT-derived blastocysts shows that the minicircles are maintained through SCNT and early embryonic development, which raises the prospect of using cell lines with episomal minicircles for the generation of transgenic animals. More... »
PAGES1-10
http://scigraph.springernature.com/pub.10.1007/s11033-019-04624-x
DOIhttp://dx.doi.org/10.1007/s11033-019-04624-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1111764491
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30694456
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Aberdeen",
"id": "https://www.grid.ac/institutes/grid.7107.1",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand",
"Rowett Institute, University of Aberdeen, AB25 2ZD, Aberdeen, UK"
],
"type": "Organization"
},
"familyName": "Wagner",
"givenName": "Stefan",
"type": "Person"
},
{
"affiliation": {
"alternateName": "AgResearch (New Zealand)",
"id": "https://www.grid.ac/institutes/grid.417738.e",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand"
],
"type": "Organization"
},
"familyName": "McCracken",
"givenName": "Judi",
"type": "Person"
},
{
"affiliation": {
"alternateName": "AgResearch (New Zealand)",
"id": "https://www.grid.ac/institutes/grid.417738.e",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand"
],
"type": "Organization"
},
"familyName": "Bruszies",
"givenName": "Sabine",
"type": "Person"
},
{
"affiliation": {
"alternateName": "AgResearch (New Zealand)",
"id": "https://www.grid.ac/institutes/grid.417738.e",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand"
],
"type": "Organization"
},
"familyName": "Broadhurst",
"givenName": "Ric",
"type": "Person"
},
{
"affiliation": {
"alternateName": "AgResearch (New Zealand)",
"id": "https://www.grid.ac/institutes/grid.417738.e",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand"
],
"type": "Organization"
},
"familyName": "Wells",
"givenName": "David N.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "AgResearch (New Zealand)",
"id": "https://www.grid.ac/institutes/grid.417738.e",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand"
],
"type": "Organization"
},
"familyName": "Oback",
"givenName": "Bj\u00f6rn",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Hannover Medical School",
"id": "https://www.grid.ac/institutes/grid.10423.34",
"name": [
"Hannover Medical School (MHH), Carl-Neuberg-Strasse 1, 30625, Hannover, Germany"
],
"type": "Organization"
},
"familyName": "Bode",
"givenName": "J\u00fcrgen",
"type": "Person"
},
{
"affiliation": {
"alternateName": "AgResearch (New Zealand)",
"id": "https://www.grid.ac/institutes/grid.417738.e",
"name": [
"AgResearch Limited, Ruakura Research Centre, 3240, Hamilton, New Zealand"
],
"type": "Organization"
},
"familyName": "Laible",
"givenName": "G\u00f6tz",
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1038/sj.mt.6300177",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000672225"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1038/sj.mt.6300234",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003366175"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0604938103",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004244324"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1080/08880010701773829",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005050171"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc1122",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010076917",
"https://doi.org/10.1038/nrc1122"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrc1122",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010076917",
"https://doi.org/10.1038/nrc1122"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1472-6750-10-20",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013115975",
"https://doi.org/10.1186/1472-6750-10-20"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/geno.1995.1274",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015192328"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0093-691x(02)01273-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016773080"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0093-691x(02)01273-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016773080"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302877",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018335408",
"https://doi.org/10.1038/sj.gt.3302877"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.gt.3302877",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018335408",
"https://doi.org/10.1038/sj.gt.3302877"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.88.22.10327",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020230892"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jmb.2005.11.073",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023439086"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jmb.2005.11.073",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023439086"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1024962308071",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024168115",
"https://doi.org/10.1023/a:1024962308071"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/b:mole.0000031363.35839.46",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025869581",
"https://doi.org/10.1023/b:mole.0000031363.35839.46"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/27.2.426",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026370844"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11427-016-0067-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028065889",
"https://doi.org/10.1007/s11427-016-0067-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11427-016-0067-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028065889",
"https://doi.org/10.1007/s11427-016-0067-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4614-3209-8_88",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029397506",
"https://doi.org/10.1007/978-1-4614-3209-8_88"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/gt.2008.113",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035183426",
"https://doi.org/10.1038/gt.2008.113"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0401355101",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036358425"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.00718-06",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036554661"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0035619",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036631520"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng0198-56",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039448670",
"https://doi.org/10.1038/ng0198-56"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkm570",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043387592"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt783",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044254589",
"https://doi.org/10.1038/nbt783"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt783",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044254589",
"https://doi.org/10.1038/nbt783"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jmb.2009.11.066",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047616121"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gku082",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048407717"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.gene.2015.01.032",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050129314"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/24.15.3118",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050571566"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/embo-reports/kvf070",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050705668"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/bs.adgen.2014.11.002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051261191"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(94)90439-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051424903"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0073138",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051532085"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/153623003321512111",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059214902"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/scd.2008.0255",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059309888"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1553545",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062490954"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1517/14712598.2011.582035",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1067588453"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1530/rep-12-0340",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1067717627"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.20506/rst.17.1.1099",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1068791619"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.20506/rst.17.1.1099",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1068791619"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2144/000113270",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069096091"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/138161212799315993",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069168031"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/138920311796391142",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069179413"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2527/jas.2006-486",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1070885813"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4314/njm.v17i3.37390",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072492292"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/hum.2017.025",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090390892"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-01-29",
"datePublishedReg": "2019-01-29",
"description": "Episomal plasmids based on a scaffold/matrix attachment region (S/MAR) are extrachromosomal DNA entities that replicate once per cell cycle and are stably maintained in cells or tissue. We generated minicircles, episomal plasmids devoid of bacterial sequences, and show that they are stably transmitted in clonal primary bovine fibroblasts without selection pressure over more than two months. Total DNA, plasmid extraction and fluorescence in situ hybridization (FISH) analyses suggest that the minicircles remained episomal and were not integrated into the genome. Minicircles survived extended periods in serum-starved cells, which indicates that ongoing transcription in non-proliferating cells is not necessary for the maintenance of S/MAR-episomes. To test whether minicircles endure the process of somatic cell nuclear transfer (SCNT), we used cell-cycle synchronized, serum-starved, minicircle-containing cells. Analysis of cells outgrown from SCNT-derived blastocysts shows that the minicircles are maintained through SCNT and early embryonic development, which raises the prospect of using cell lines with episomal minicircles for the generation of transgenic animals.",
"genre": "research_article",
"id": "sg:pub.10.1007/s11033-019-04624-x",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1018503",
"issn": [
"0301-4851",
"1573-4978"
],
"name": "Molecular Biology Reports",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "46"
}
],
"name": "Episomal minicircles persist in periods of transcriptional inactivity and can be transmitted through somatic cell nuclear transfer into bovine embryos",
"pagination": "1-10",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11033-019-04624-x"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"8150fe2b9a98af7b559ac74474ac7cb2208a50f5b3e77f75d7f7914db556a37d"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1111764491"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0403234"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30694456"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11033-019-04624-x",
"https://app.dimensions.ai/details/publication/pub.1111764491"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T09:25",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56191_00000006.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1007%2Fs11033-019-04624-x"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11033-019-04624-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11033-019-04624-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11033-019-04624-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11033-019-04624-x'
This table displays all metadata directly associated to this object as RDF triples.
256 TRIPLES
21 PREDICATES
71 URIs
20 LITERALS
9 BLANK NODES