Cloning of two cellobiohydrolase genes from Trichodermaviride and heterogenous expression in yeast Saccharomyces cerevisiae View Full Text


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Article Info

DATE

2009-08-11

AUTHORS

Jinzhu Song, Beidong Liu, Zhihua Liu, Qian Yang

ABSTRACT

Cellobiohydrolase genes cbhI and cbhII were isolated from Trichoderma viride AS3.3711 and T. viride CICC 13038, respectively, using RT-PCR technique. The cbhI gene from T. viride AS3.3711 contains 1,542 nucleotides and encodes a 514-amino acid protein with a molecular weight of approximately 53.96 kDa. The cbhII gene from T. viride CICC 13038 was 1,413 bp in length encoding 471 amino acid residues with a molecular weight of approximately 49.55 kDa. The CBHI protein showed high homology with enzymes belonging to glycoside hydrolase family 7 and CBHII is a member of Glycoside hydrolase family 6. CBHI and CBHII play a role in the conversion of cellulose to glucose by cutting the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. The two cellobiohydrolase (CBHI, CBHII) genes were successfully expressed in Saccharomyces cerevisiae H158. Maximal activities of transformants Sc-cbhI and Sc-cbhII were 0.03 and 0.089 units ml−1 under galactose induction, respectively. The optimal temperatures of the recombinant enzymes (CBHI, CBHII) were 60 and 70°C, respectively. The optimal pHs of recombinant enzymes CBHI and CBHII were at pH 5.8 and 5.0, respectively. More... »

PAGES

2135-2140

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URI

http://scigraph.springernature.com/pub.10.1007/s11033-009-9683-3

DOI

http://dx.doi.org/10.1007/s11033-009-9683-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022381330

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19669931


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