Frequency of a natural truncated allele of MdMLO19 in the germplasm of Malus domestica View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-01

AUTHORS

Stefano Pessina, Luisa Palmieri, Luca Bianco, Jennifer Gassmann, Eric van de Weg, Richard G. F. Visser, Pierluigi Magnago, Henk J. Schouten, Yuling Bai, R. Riccardo Velasco, Mickael Malnoy

ABSTRACT

Podosphaera leucotricha is the causal agent of powdery mildew (PM) in apple. To reduce the amount of fungicides required to control this pathogen, the development of resistant apple cultivars should become a priority. Resistance to PM was achieved in various crops by knocking out specific members of the MLO gene family that are responsible for PM susceptibility (S-genes). In apple, the knockdown of MdMLO19 resulted in PM resistance. However, since gene silencing technologies such as RNAi are perceived unfavorably in Europe, a different approach that exploits this type of resistance is needed. This work evaluates the presence of non-functional naturally occurring alleles of MdMLO19 in apple germplasm. The screening of the re-sequencing data of 63 apple individuals led to the identification of 627 single nucleotide polymorphisms (SNPs) in five MLO genes (MdMLO5, MdMLO7, MdMLO11, MdMLO18, and MdMLO19), 127 of which were located in exons. The T-1201 insertion of a single nucleotide in MdMLO19 caused the formation of an early stop codon, resulting in a truncated protein lacking 185 amino acids, including the calmodulin-binding domain. The presence of the insertion was evaluated in 115 individuals. It was heterozygous in 64 and homozygous in 25. Twelve of the 25 individuals carrying the insertion in homozygosity were susceptible to PM. After barley, pea, cucumber, and tomato, apple would be the fifth species for which a natural non-functional mlo allele has been found. More... »

PAGES

7

References to SciGraph publications

  • 2014-12. Characterization of the MLO gene family in Rosaceae and gene expression analysis in Malus domestica in BMC GENOMICS
  • 2010-12. Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley Mlo protein in BMC PLANT BIOLOGY
  • 2004-08. A barley cultivation-associated polymorphism conveys resistance to powdery mildew in NATURE
  • 1992-01. Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley in EUPHYTICA
  • 2006-06. Conserved requirement for a plant host cell protein in powdery mildew pathogenesis in NATURE GENETICS
  • 1977-05. Preponderance of synonymous changes as evidence for the neutral theory of molecular evolution in NATURE
  • 2014-09. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew in NATURE BIOTECHNOLOGY
  • 1996-06. The staden sequence analysis package in MOLECULAR BIOTECHNOLOGY
  • 2010-01. Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance in MOLECULAR BREEDING
  • 2002-03. Calmodulin interacts with MLO protein to regulate defence against mildew in barley in NATURE
  • 2011-11. Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and Genome Analyzer systems in GENOME BIOLOGY
  • 2015-12. A transposable element insertion in the susceptibility gene CsaMLO8 results in hypocotyl resistance to powdery mildew in cucumber in BMC PLANT BIOLOGY
  • 2016-12. Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine in HORTICULTURE RESEARCH
  • 2011-12. Genotyping of pedigreed apple breeding material with a genome-covering set of SSRs: trueness-to-type of cultivars and their parentages in MOLECULAR BREEDING
  • 2011-12. Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus in THEORETICAL AND APPLIED GENETICS
  • 2007. The Gap between Science and Perception: The Case of Plant Biotechnology in Europe in GREEN GENE TECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11032-016-0610-8

    DOI

    http://dx.doi.org/10.1007/s11032-016-0610-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006027732

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/28127253


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all\u2019Adige, Italy", 
                "Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pessina", 
            "givenName": "Stefano", 
            "id": "sg:person.01304157720.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304157720.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fondazione Edmund Mach", 
              "id": "https://www.grid.ac/institutes/grid.424414.3", 
              "name": [
                "Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all\u2019Adige, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Palmieri", 
            "givenName": "Luisa", 
            "id": "sg:person.01003133365.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003133365.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fondazione Edmund Mach", 
              "id": "https://www.grid.ac/institutes/grid.424414.3", 
              "name": [
                "Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all\u2019Adige, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bianco", 
            "givenName": "Luca", 
            "id": "sg:person.01065537006.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065537006.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Agroscope", 
              "id": "https://www.grid.ac/institutes/grid.417771.3", 
              "name": [
                "Agroscope, Institute for Plant Production Sciences IPS, Schloss 1, 8820, W\u00e4denswil, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gassmann", 
            "givenName": "Jennifer", 
            "id": "sg:person.0641712377.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641712377.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van de Weg", 
            "givenName": "Eric", 
            "id": "sg:person.0765542171.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765542171.21"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Visser", 
            "givenName": "Richard G. F.", 
            "id": "sg:person.07374343732.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07374343732.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fondazione Edmund Mach", 
              "id": "https://www.grid.ac/institutes/grid.424414.3", 
              "name": [
                "Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all\u2019Adige, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Magnago", 
            "givenName": "Pierluigi", 
            "id": "sg:person.01260524676.07", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260524676.07"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schouten", 
            "givenName": "Henk J.", 
            "id": "sg:person.01077167626.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077167626.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bai", 
            "givenName": "Yuling", 
            "id": "sg:person.0757673773.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757673773.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fondazione Edmund Mach", 
              "id": "https://www.grid.ac/institutes/grid.424414.3", 
              "name": [
                "Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all\u2019Adige, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Riccardo Velasco", 
            "givenName": "R.", 
            "id": "sg:person.0702777727.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702777727.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fondazione Edmund Mach", 
              "id": "https://www.grid.ac/institutes/grid.424414.3", 
              "name": [
                "Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all\u2019Adige, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Malnoy", 
            "givenName": "Mickael", 
            "id": "sg:person.01074410551.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074410551.54"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/267275a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004682902", 
              "https://doi.org/10.1038/267275a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/267275a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004682902", 
              "https://doi.org/10.1038/267275a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2164-15-618", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005240247", 
              "https://doi.org/10.1186/1471-2164-15-618"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/tpj.13145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007165598"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/pbi.12562", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007822755"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20040993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008624732"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20040993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008624732"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-294x.1999.00669.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010635305"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.010954", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013466245"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/416447a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017594446", 
              "https://doi.org/10.1038/416447a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/416447a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017594446", 
              "https://doi.org/10.1038/416447a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1071/fp08173", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018814896"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bst0330389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022302646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bst0330389", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022302646"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/10_2007_055", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023066887", 
              "https://doi.org/10.1007/10_2007_055"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1806", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023434143", 
              "https://doi.org/10.1038/ng1806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng1806", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023434143", 
              "https://doi.org/10.1038/ng1806"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0110377", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024647158"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2229-10-31", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024928758", 
              "https://doi.org/10.1186/1471-2229-10-31"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2011-12-11-r112", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027516867", 
              "https://doi.org/10.1186/gb-2011-12-11-r112"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/hortres.2016.16", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028684542", 
              "https://doi.org/10.1038/hortres.2016.16"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02900361", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032182823", 
              "https://doi.org/10.1007/bf02900361"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02900361", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032182823", 
              "https://doi.org/10.1007/bf02900361"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.sajb.2014.01.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033220892"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/nph.12889", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034474393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature02781", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034610863", 
              "https://doi.org/10.1038/nature02781"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature02781", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034610863", 
              "https://doi.org/10.1038/nature02781"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.phyto.46.081407.104740", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035435315"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2969", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037367582", 
              "https://doi.org/10.1038/nbt.2969"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12870-015-0635-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041470755", 
              "https://doi.org/10.1186/s12870-015-0635-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00023919", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043190604", 
              "https://doi.org/10.1007/bf00023919"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00023919", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043190604", 
              "https://doi.org/10.1007/bf00023919"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-011-1677-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045993344", 
              "https://doi.org/10.1007/s00122-011-1677-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-010-9502-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048160832", 
              "https://doi.org/10.1007/s11032-010-9502-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-009-9323-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051777686", 
              "https://doi.org/10.1007/s11032-009-9323-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11032-009-9323-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051777686", 
              "https://doi.org/10.1007/s11032-009-9323-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0070723", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051839388"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/aobpla/plv117", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052198767"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.plantsci.2009.12.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053578077"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi-21-1-0030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060078660"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/pdis.2000.84.11.1171", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060095965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/pdis.2000.84.11.1171", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060095965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/1938672", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069662568"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2017-01", 
        "datePublishedReg": "2017-01-01", 
        "description": "Podosphaera leucotricha is the causal agent of powdery mildew (PM) in apple. To reduce the amount of fungicides required to control this pathogen, the development of resistant apple cultivars should become a priority. Resistance to PM was achieved in various crops by knocking out specific members of the MLO gene family that are responsible for PM susceptibility (S-genes). In apple, the knockdown of MdMLO19 resulted in PM resistance. However, since gene silencing technologies such as RNAi are perceived unfavorably in Europe, a different approach that exploits this type of resistance is needed. This work evaluates the presence of non-functional naturally occurring alleles of MdMLO19 in apple germplasm. The screening of the re-sequencing data of 63 apple individuals led to the identification of 627 single nucleotide polymorphisms (SNPs) in five MLO genes (MdMLO5, MdMLO7, MdMLO11, MdMLO18, and MdMLO19), 127 of which were located in exons. The T-1201 insertion of a single nucleotide in MdMLO19 caused the formation of an early stop codon, resulting in a truncated protein lacking 185 amino acids, including the calmodulin-binding domain. The presence of the insertion was evaluated in 115 individuals. It was heterozygous in 64 and homozygous in 25. Twelve of the 25 individuals carrying the insertion in homozygosity were susceptible to PM. After barley, pea, cucumber, and tomato, apple would be the fifth species for which a natural non-functional mlo allele has been found.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s11032-016-0610-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.3789852", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1114043", 
            "issn": [
              "1380-3743", 
              "1572-9788"
            ], 
            "name": "Molecular Breeding", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "37"
          }
        ], 
        "name": "Frequency of a natural truncated allele of MdMLO19 in the germplasm of Malus domestica", 
        "pagination": "7", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "1cb67b902d49d14db987ebd46d27915082df0554b0aea280789875ad245ab748"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "28127253"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9506703"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11032-016-0610-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1006027732"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11032-016-0610-8", 
          "https://app.dimensions.ai/details/publication/pub.1006027732"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T10:01", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89819_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs11032-016-0610-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11032-016-0610-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11032-016-0610-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11032-016-0610-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11032-016-0610-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    263 TRIPLES      21 PREDICATES      62 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11032-016-0610-8 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author Nb8fd1a43e0e2479e88abdf841c08ba0f
    4 schema:citation sg:pub.10.1007/10_2007_055
    5 sg:pub.10.1007/bf00023919
    6 sg:pub.10.1007/bf02900361
    7 sg:pub.10.1007/s00122-011-1677-6
    8 sg:pub.10.1007/s11032-009-9323-6
    9 sg:pub.10.1007/s11032-010-9502-5
    10 sg:pub.10.1038/267275a0
    11 sg:pub.10.1038/416447a
    12 sg:pub.10.1038/hortres.2016.16
    13 sg:pub.10.1038/nature02781
    14 sg:pub.10.1038/nbt.2969
    15 sg:pub.10.1038/ng1806
    16 sg:pub.10.1186/1471-2164-15-618
    17 sg:pub.10.1186/1471-2229-10-31
    18 sg:pub.10.1186/gb-2011-12-11-r112
    19 sg:pub.10.1186/s12870-015-0635-x
    20 https://doi.org/10.1016/j.plantsci.2009.12.005
    21 https://doi.org/10.1016/j.sajb.2014.01.013
    22 https://doi.org/10.1042/bj20040993
    23 https://doi.org/10.1042/bst0330389
    24 https://doi.org/10.1046/j.1365-294x.1999.00669.x
    25 https://doi.org/10.1071/fp08173
    26 https://doi.org/10.1093/aobpla/plv117
    27 https://doi.org/10.1094/mpmi-21-1-0030
    28 https://doi.org/10.1094/pdis.2000.84.11.1171
    29 https://doi.org/10.1104/pp.010954
    30 https://doi.org/10.1111/nph.12889
    31 https://doi.org/10.1111/pbi.12562
    32 https://doi.org/10.1111/tpj.13145
    33 https://doi.org/10.1146/annurev.phyto.46.081407.104740
    34 https://doi.org/10.1371/journal.pone.0070723
    35 https://doi.org/10.1371/journal.pone.0110377
    36 https://doi.org/10.2307/1938672
    37 schema:datePublished 2017-01
    38 schema:datePublishedReg 2017-01-01
    39 schema:description <i>Podosphaera leucotricha</i> is the causal agent of powdery mildew (PM) in apple. To reduce the amount of fungicides required to control this pathogen, the development of resistant apple cultivars should become a priority. Resistance to PM was achieved in various crops by knocking out specific members of the <i>MLO</i> gene family that are responsible for PM susceptibility (S-genes). In apple, the knockdown of <i>MdMLO19</i> resulted in PM resistance. However, since gene silencing technologies such as RNAi are perceived unfavorably in Europe, a different approach that exploits this type of resistance is needed. This work evaluates the presence of non-functional naturally occurring alleles of <i>MdMLO19</i> in apple germplasm. The screening of the re-sequencing data of 63 apple individuals led to the identification of 627 single nucleotide polymorphisms (SNPs) in five <i>MLO</i> genes (<i>MdMLO5, MdMLO7, MdMLO11, MdMLO18</i>, and <i>MdMLO19</i>), 127 of which were located in exons. The T-1201 insertion of a single nucleotide in <i>MdMLO19</i> caused the formation of an early stop codon, resulting in a truncated protein lacking 185 amino acids, including the calmodulin-binding domain. The presence of the insertion was evaluated in 115 individuals. It was heterozygous in 64 and homozygous in 25. Twelve of the 25 individuals carrying the insertion in homozygosity were susceptible to PM. After barley, pea, cucumber, and tomato, apple would be the fifth species for which a natural non-functional <i>mlo</i> allele has been found.
    40 schema:genre research_article
    41 schema:inLanguage en
    42 schema:isAccessibleForFree true
    43 schema:isPartOf N92e8edc725e14c7da780b3723ceaec28
    44 Nd09eb7894f04464a9fc6375445a4a4ce
    45 sg:journal.1114043
    46 schema:name Frequency of a natural truncated allele of MdMLO19 in the germplasm of Malus domestica
    47 schema:pagination 7
    48 schema:productId N1708123db8dd4f309fd66d9113dac3da
    49 N5de6ec1e939c4ad385184854128b20b3
    50 Nb0eb2e87341040ad8e7deb1043d244d7
    51 Nc3c947be86064faab2c5011f3cab5f9f
    52 Nc6924467122d40c5bdaa192159929ad1
    53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006027732
    54 https://doi.org/10.1007/s11032-016-0610-8
    55 schema:sdDatePublished 2019-04-11T10:01
    56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    57 schema:sdPublisher Nc3114e59f33c40cba4c56ab81a65ee46
    58 schema:url https://link.springer.com/10.1007%2Fs11032-016-0610-8
    59 sgo:license sg:explorer/license/
    60 sgo:sdDataset articles
    61 rdf:type schema:ScholarlyArticle
    62 N1708123db8dd4f309fd66d9113dac3da schema:name nlm_unique_id
    63 schema:value 9506703
    64 rdf:type schema:PropertyValue
    65 N27a521684fc34db783dd03e0f3652319 rdf:first sg:person.01074410551.54
    66 rdf:rest rdf:nil
    67 N44a7a5eda3d74ec8960153be33d208da rdf:first sg:person.01260524676.07
    68 rdf:rest Ne08ebd5ef0a1408f894871d8284942d5
    69 N4f0f8609313d4378b0dbaa1d03fbed25 rdf:first sg:person.0765542171.21
    70 rdf:rest Na9422f23288e41dbb9b78302bbac8676
    71 N4fb5b19ac91b4873b7f2d163baadc05a rdf:first sg:person.0702777727.55
    72 rdf:rest N27a521684fc34db783dd03e0f3652319
    73 N5de6ec1e939c4ad385184854128b20b3 schema:name doi
    74 schema:value 10.1007/s11032-016-0610-8
    75 rdf:type schema:PropertyValue
    76 N80cb0eee208046a297282a9bd4edc155 rdf:first sg:person.0641712377.01
    77 rdf:rest N4f0f8609313d4378b0dbaa1d03fbed25
    78 N92e8edc725e14c7da780b3723ceaec28 schema:issueNumber 1
    79 rdf:type schema:PublicationIssue
    80 Na526ea5faea04283903b52133e3ac0a6 rdf:first sg:person.0757673773.09
    81 rdf:rest N4fb5b19ac91b4873b7f2d163baadc05a
    82 Na9422f23288e41dbb9b78302bbac8676 rdf:first sg:person.07374343732.28
    83 rdf:rest N44a7a5eda3d74ec8960153be33d208da
    84 Nb0eb2e87341040ad8e7deb1043d244d7 schema:name readcube_id
    85 schema:value 1cb67b902d49d14db987ebd46d27915082df0554b0aea280789875ad245ab748
    86 rdf:type schema:PropertyValue
    87 Nb8fd1a43e0e2479e88abdf841c08ba0f rdf:first sg:person.01304157720.31
    88 rdf:rest Nfc45ea93e06f4944b1164391e9db8090
    89 Nc3114e59f33c40cba4c56ab81a65ee46 schema:name Springer Nature - SN SciGraph project
    90 rdf:type schema:Organization
    91 Nc3c947be86064faab2c5011f3cab5f9f schema:name pubmed_id
    92 schema:value 28127253
    93 rdf:type schema:PropertyValue
    94 Nc6924467122d40c5bdaa192159929ad1 schema:name dimensions_id
    95 schema:value pub.1006027732
    96 rdf:type schema:PropertyValue
    97 Ncd8f17d745134fd693c8fc7bc3b905d5 rdf:first sg:person.01065537006.16
    98 rdf:rest N80cb0eee208046a297282a9bd4edc155
    99 Nd09eb7894f04464a9fc6375445a4a4ce schema:volumeNumber 37
    100 rdf:type schema:PublicationVolume
    101 Ne08ebd5ef0a1408f894871d8284942d5 rdf:first sg:person.01077167626.52
    102 rdf:rest Na526ea5faea04283903b52133e3ac0a6
    103 Nfc45ea93e06f4944b1164391e9db8090 rdf:first sg:person.01003133365.23
    104 rdf:rest Ncd8f17d745134fd693c8fc7bc3b905d5
    105 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    106 schema:name Biological Sciences
    107 rdf:type schema:DefinedTerm
    108 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    109 schema:name Genetics
    110 rdf:type schema:DefinedTerm
    111 sg:grant.3789852 http://pending.schema.org/fundedItem sg:pub.10.1007/s11032-016-0610-8
    112 rdf:type schema:MonetaryGrant
    113 sg:journal.1114043 schema:issn 1380-3743
    114 1572-9788
    115 schema:name Molecular Breeding
    116 rdf:type schema:Periodical
    117 sg:person.01003133365.23 schema:affiliation https://www.grid.ac/institutes/grid.424414.3
    118 schema:familyName Palmieri
    119 schema:givenName Luisa
    120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003133365.23
    121 rdf:type schema:Person
    122 sg:person.01065537006.16 schema:affiliation https://www.grid.ac/institutes/grid.424414.3
    123 schema:familyName Bianco
    124 schema:givenName Luca
    125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065537006.16
    126 rdf:type schema:Person
    127 sg:person.01074410551.54 schema:affiliation https://www.grid.ac/institutes/grid.424414.3
    128 schema:familyName Malnoy
    129 schema:givenName Mickael
    130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074410551.54
    131 rdf:type schema:Person
    132 sg:person.01077167626.52 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    133 schema:familyName Schouten
    134 schema:givenName Henk J.
    135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077167626.52
    136 rdf:type schema:Person
    137 sg:person.01260524676.07 schema:affiliation https://www.grid.ac/institutes/grid.424414.3
    138 schema:familyName Magnago
    139 schema:givenName Pierluigi
    140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260524676.07
    141 rdf:type schema:Person
    142 sg:person.01304157720.31 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    143 schema:familyName Pessina
    144 schema:givenName Stefano
    145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01304157720.31
    146 rdf:type schema:Person
    147 sg:person.0641712377.01 schema:affiliation https://www.grid.ac/institutes/grid.417771.3
    148 schema:familyName Gassmann
    149 schema:givenName Jennifer
    150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641712377.01
    151 rdf:type schema:Person
    152 sg:person.0702777727.55 schema:affiliation https://www.grid.ac/institutes/grid.424414.3
    153 schema:familyName Riccardo Velasco
    154 schema:givenName R.
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0702777727.55
    156 rdf:type schema:Person
    157 sg:person.07374343732.28 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    158 schema:familyName Visser
    159 schema:givenName Richard G. F.
    160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07374343732.28
    161 rdf:type schema:Person
    162 sg:person.0757673773.09 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    163 schema:familyName Bai
    164 schema:givenName Yuling
    165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757673773.09
    166 rdf:type schema:Person
    167 sg:person.0765542171.21 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    168 schema:familyName van de Weg
    169 schema:givenName Eric
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0765542171.21
    171 rdf:type schema:Person
    172 sg:pub.10.1007/10_2007_055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023066887
    173 https://doi.org/10.1007/10_2007_055
    174 rdf:type schema:CreativeWork
    175 sg:pub.10.1007/bf00023919 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043190604
    176 https://doi.org/10.1007/bf00023919
    177 rdf:type schema:CreativeWork
    178 sg:pub.10.1007/bf02900361 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032182823
    179 https://doi.org/10.1007/bf02900361
    180 rdf:type schema:CreativeWork
    181 sg:pub.10.1007/s00122-011-1677-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045993344
    182 https://doi.org/10.1007/s00122-011-1677-6
    183 rdf:type schema:CreativeWork
    184 sg:pub.10.1007/s11032-009-9323-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051777686
    185 https://doi.org/10.1007/s11032-009-9323-6
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1007/s11032-010-9502-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048160832
    188 https://doi.org/10.1007/s11032-010-9502-5
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/267275a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004682902
    191 https://doi.org/10.1038/267275a0
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1038/416447a schema:sameAs https://app.dimensions.ai/details/publication/pub.1017594446
    194 https://doi.org/10.1038/416447a
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1038/hortres.2016.16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028684542
    197 https://doi.org/10.1038/hortres.2016.16
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1038/nature02781 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034610863
    200 https://doi.org/10.1038/nature02781
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1038/nbt.2969 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037367582
    203 https://doi.org/10.1038/nbt.2969
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1038/ng1806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023434143
    206 https://doi.org/10.1038/ng1806
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1186/1471-2164-15-618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005240247
    209 https://doi.org/10.1186/1471-2164-15-618
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1186/1471-2229-10-31 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024928758
    212 https://doi.org/10.1186/1471-2229-10-31
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1186/gb-2011-12-11-r112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027516867
    215 https://doi.org/10.1186/gb-2011-12-11-r112
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1186/s12870-015-0635-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041470755
    218 https://doi.org/10.1186/s12870-015-0635-x
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/j.plantsci.2009.12.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053578077
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/j.sajb.2014.01.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033220892
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1042/bj20040993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008624732
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1042/bst0330389 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022302646
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1046/j.1365-294x.1999.00669.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010635305
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1071/fp08173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018814896
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1093/aobpla/plv117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052198767
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1094/mpmi-21-1-0030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060078660
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1094/pdis.2000.84.11.1171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060095965
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1104/pp.010954 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013466245
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1111/nph.12889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034474393
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1111/pbi.12562 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007822755
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1111/tpj.13145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007165598
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1146/annurev.phyto.46.081407.104740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035435315
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1371/journal.pone.0070723 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051839388
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1371/journal.pone.0110377 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024647158
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.2307/1938672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069662568
    253 rdf:type schema:CreativeWork
    254 https://www.grid.ac/institutes/grid.417771.3 schema:alternateName Agroscope
    255 schema:name Agroscope, Institute for Plant Production Sciences IPS, Schloss 1, 8820, Wädenswil, Switzerland
    256 rdf:type schema:Organization
    257 https://www.grid.ac/institutes/grid.424414.3 schema:alternateName Fondazione Edmund Mach
    258 schema:name Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all’Adige, Italy
    259 rdf:type schema:Organization
    260 https://www.grid.ac/institutes/grid.4818.5 schema:alternateName Wageningen University & Research
    261 schema:name Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, 38010, San Michele all’Adige, Italy
    262 Wageningen UR Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700, AJ Wageningen, the Netherlands
    263 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...