Particle bombardment and the genetic enhancement of crops: myths and realities View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-04

AUTHORS

Fredy Altpeter, Niranjan Baisakh, Roger Beachy, Ralph Bock, Teresa Capell, Paul Christou, Henry Daniell, Karabi Datta, Swapan Datta, Philip J. Dix, Claude Fauquet, Ning Huang, Ajay Kohli, Hans Mooibroek, Liz Nicholson, Thi Thanh Nguyen, Gregory Nugent, Krit Raemakers, Andrea Romano, David A. Somers, Eva Stoger, Nigel Taylor, Richard Visser

ABSTRACT

DNA transfer by particle bombardment makes use of physical processes to achieve the transformation of crop plants. There is no dependence on bacteria, so the limitations inherent in organisms such as Agrobacterium tumefaciens do not apply. The absence of biological constraints, at least until DNA has entered the plant cell, means that particle bombardment is a versatile and effective transformation method, not limited by cell type, species or genotype. There are no intrinsic vector requirements so transgenes of any size and arrangement can be introduced, and multiple gene cotransformation is straightforward. The perceived disadvantages of particle bombardment compared to Agrobacterium-mediated transformation, i.e. the tendency to generate large transgene arrays containing rearranged and broken transgene copies, are not borne out by the recent detailed structural analysis of transgene loci produced by each of the methods. There is also little evidence for major differences in the levels of transgene instability and silencing when these transformation methods are compared in agriculturally important cereals and legumes, and other non-model systems. Indeed, a major advantage of particle bombardment is that the delivered DNA can be manipulated to influence the quality and structure of the resultant transgene loci. This has been demonstrated in recently reported strategies that favor the recovery of transgenic plants containing intact, single-copy integration events, and demonstrating high-level transgene expression. At the current time, particle bombardment is the most efficient way to achieve plastid transformation in plants and is the only method so far used to achieve mitochondrial transformation. In this review, we discuss recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell. We also discuss some of the most important applications of this technology including the deployment of transgenic plants under field conditions. More... »

PAGES

305-327

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  • Journal

    TITLE

    Molecular Breeding

    ISSUE

    3

    VOLUME

    15

    Author Affiliations

  • Laboratory of Molecular Plant Physiology, Agronomy Department, University of Florida – IFAS, 2191 McCarty Hall, P.O. Box 110300, 32611-0300, Gainesville, FL, USA
  • Division of Plant Breeding, Genetics, and Biochemistry, International Rice Research Institute, DAPO Box 7777, Metro Manila, The Philippines
  • Danforth Plant Science Center, 975 North Warson Road, St. Louis, USA
  • Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476, Golm, Germany
  • Department de Produccio Vegetal i Ciencia Forestal, Universitat de Lleida, Av. Alcalde Rovira Roure, 191 E-25198, Lleida, Spain
  • Department of Molecular Biology and Microbiology, University of Central Florida, 32816-2364, Orlando, FL, USA
  • Institute of Bioengineering and Agroecology,Department of Biology, National University of Ireland Maynooth, Maynooth Co., Kildare, Ireland
  • International Laboratory for Tropical Agricultural Biotechnology (ILTAB), Danforth Plant Science Center, 975 North Warson Road, St. Louis, USA
  • Ventria Bioscience, 4110 North Freeway, 95834, Sacramento, CA, USA
  • Institute of Research on Environment and Sustainability (IRES), School of Biology, University of Newcastle upon Tyne, Devonshire Building, NE1 7RU, Newcastle upon Tyne, UK
  • Agrotechnology and Food Innovations B.V., Department of Bioconversion, Wageningen University and Research Centre, Bornsesteeg 59, 6708, PD Wageningen, The Netherlands
  • John Innes Centre, Norwich Research Park, NR4 7UH, Norwich, UK
  • Department of Primary Industries, Primary Industries Research Victoria, Plant Biotechnology Centre, La Trobe University, 3086, Bundoora, Victoria, Australia
  • Laboratory of Plant Breeding, Wageningen University and Research Centre, P.O.B. 386, 6700, AJ Wageningen, The Netherlands
  • Research Institute GROW, Department of Obstetrics and Gynaecology, University Hospital of Maastricht, P. Debyelaan 25, 6202 AZ, Maastricht, The Netherlands
  • Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota, USA
  • Institute for Molecular Biotechnology, Biology VII, RWTH Aachen, Worringerweg 1, 52074, Aachen, Germany
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11032-004-8001-y

    DOI

    http://dx.doi.org/10.1007/s11032-004-8001-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048042052


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    105 schema:description DNA transfer by particle bombardment makes use of physical processes to achieve the transformation of crop plants. There is no dependence on bacteria, so the limitations inherent in organisms such as Agrobacterium tumefaciens do not apply. The absence of biological constraints, at least until DNA has entered the plant cell, means that particle bombardment is a versatile and effective transformation method, not limited by cell type, species or genotype. There are no intrinsic vector requirements so transgenes of any size and arrangement can be introduced, and multiple gene cotransformation is straightforward. The perceived disadvantages of particle bombardment compared to Agrobacterium-mediated transformation, i.e. the tendency to generate large transgene arrays containing rearranged and broken transgene copies, are not borne out by the recent detailed structural analysis of transgene loci produced by each of the methods. There is also little evidence for major differences in the levels of transgene instability and silencing when these transformation methods are compared in agriculturally important cereals and legumes, and other non-model systems. Indeed, a major advantage of particle bombardment is that the delivered DNA can be manipulated to influence the quality and structure of the resultant transgene loci. This has been demonstrated in recently reported strategies that favor the recovery of transgenic plants containing intact, single-copy integration events, and demonstrating high-level transgene expression. At the current time, particle bombardment is the most efficient way to achieve plastid transformation in plants and is the only method so far used to achieve mitochondrial transformation. In this review, we discuss recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell. We also discuss some of the most important applications of this technology including the deployment of transgenic plants under field conditions.
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