Ontology type: schema:ScholarlyArticle
2019-05
AUTHORSStuart R. Green, Kenneth B. Storey
ABSTRACTCarbamoyl phosphate synthetase I (CPS1) represents an important regulatory enzyme of the urea cycle that mediates the ATP-driven reaction ligating ammonium, carbonate, and phosphate to form carbamoyl phosphate. The freeze-tolerant wood frog (Rana sylvatica or Lithobates sylvaticus) accumulates high concentrations of urea during bouts of freezing to detoxify any ammonia generated and to contribute as a cryoprotectant thereby helping to avoid freeze damage to cells. Purification of CPS1 to homogeneity from wood frog liver was performed in control and frozen wood frogs by a three-step chromatographic process. The affinity of CPS1 for its three substrates was tested in the purified control and freeze-exposed enzyme under a variety of conditions including the presence and absence of the natural cryoprotectants urea and glucose. The results demonstrated that affinity for ammonium was higher in the freeze-exposed CPS1 (1.26-fold) and that with the addition of 400 mM glucose it displayed higher affinity for ATP (1.30-fold) and the obligate activator N-acetylglutamate (1.24-fold). Denaturation studies demonstrated the freeze-exposed enzyme was less thermally stable than the control with an unfolding temperature approximately 1.5 °C lower (52.9 °C for frozen and 54.4 °C for control). The control form of CPS1 had a significantly higher degree of glutarylated lysine residues (1.42-fold increase) relative to the frozen. The results suggest that CPS1 activation and maintenance of urea cycle activity despite the hypometabolic conditions associated with freezing are important aspects in the metabolic survival strategies of the wood frog. More... »
PAGES29-39
http://scigraph.springernature.com/pub.10.1007/s11010-018-3468-8
DOIhttp://dx.doi.org/10.1007/s11010-018-3468-8
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1109816459
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30421312
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Carleton University",
"id": "https://www.grid.ac/institutes/grid.34428.39",
"name": [
"Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada"
],
"type": "Organization"
},
"familyName": "Green",
"givenName": "Stuart R.",
"id": "sg:person.07557157341.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07557157341.49"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Carleton University",
"id": "https://www.grid.ac/institutes/grid.34428.39",
"name": [
"Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada"
],
"type": "Organization"
},
"familyName": "Storey",
"givenName": "Kenneth B.",
"id": "sg:person.0601365426.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601365426.50"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf01075719",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001617051",
"https://doi.org/10.1007/bf01075719"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf01075719",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001617051",
"https://doi.org/10.1007/bf01075719"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1139/z87-315",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004630398"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00360-014-0824-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007695889",
"https://doi.org/10.1007/s00360-014-0824-1"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-9861(91)90214-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011868368"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jez.436",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013657193"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.tibs.2010.09.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016429245"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cbpb.2016.08.004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016462146"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3390/ijms12128406",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017632665"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1242/jeb.019695",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018647211"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1242/jeb.01859",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018797732"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3390/ijms130911643",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020091176"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mmbr.65.1.1-43.2001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020213941"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0117234",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020569994"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0117234",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020569994"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/cb3001793",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021289198"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1242/jeb.065961",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022801431"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7717/peerj.2436",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024337002"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(76)90527-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025529346"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1203/00006450-199004000-00020",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028174427",
"https://doi.org/10.1203/00006450-199004000-00020"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1670/0022-1511(2003)037[0390:thuawd]2.0.co;2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029510492"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.7717/peerj.558",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030395715"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0300-9629(96)00356-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030596548"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pone.0079169",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032970226"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cmet.2014.03.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038263394"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cmet.2014.03.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038263394"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/b978-012164730-8/50212-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039780519"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbabio.2010.02.011",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043478592"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jez.479",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043560532"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1139/z86-008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044273109"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ymgme.2011.07.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045066457"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ymgme.2011.07.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045066457"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbrc.2010.01.081",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045527779"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep16950",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046445592",
"https://doi.org/10.1038/srep16950"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jez.a.54",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047331151"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1242/jeb.089342",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048755871"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10930-016-9647-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049766460",
"https://doi.org/10.1007/s10930-016-9647-0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1207861",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062464832"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4161/org.5.3.9583",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072306875"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076636206",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1152/physrev.1988.68.1.27",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079657540"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082545110",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082794776",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1152/physrev.00016.2016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083735300"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.abb.2017.10.010",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092292624"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2575",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101032884",
"https://doi.org/10.1038/nchembio.2575"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2575",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101032884",
"https://doi.org/10.1038/nchembio.2575"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2575",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101032884",
"https://doi.org/10.1038/nchembio.2575"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nchembio.2575",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101032884",
"https://doi.org/10.1038/nchembio.2575"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1098/rspb.2018.0241",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103739716"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1098/rspb.2018.0241",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103739716"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1098/rspb.2018.0241",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103739716"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-05",
"datePublishedReg": "2019-05-01",
"description": "Carbamoyl phosphate synthetase I (CPS1) represents an important regulatory enzyme of the urea cycle that mediates the ATP-driven reaction ligating ammonium, carbonate, and phosphate to form carbamoyl phosphate. The freeze-tolerant wood frog (Rana sylvatica or Lithobates sylvaticus) accumulates high concentrations of urea during bouts of freezing to detoxify any ammonia generated and to contribute as a cryoprotectant thereby helping to avoid freeze damage to cells. Purification of CPS1 to homogeneity from wood frog liver was performed in control and frozen wood frogs by a three-step chromatographic process. The affinity of CPS1 for its three substrates was tested in the purified control and freeze-exposed enzyme under a variety of conditions including the presence and absence of the natural cryoprotectants urea and glucose. The results demonstrated that affinity for ammonium was higher in the freeze-exposed CPS1 (1.26-fold) and that with the addition of 400\u00a0mM glucose it displayed higher affinity for ATP (1.30-fold) and the obligate activator N-acetylglutamate (1.24-fold). Denaturation studies demonstrated the freeze-exposed enzyme was less thermally stable than the control with an unfolding temperature approximately 1.5\u00a0\u00b0C lower (52.9\u00a0\u00b0C for frozen and 54.4\u00a0\u00b0C for control). The control form of CPS1 had a significantly higher degree of glutarylated lysine residues (1.42-fold increase) relative to the frozen. The results suggest that CPS1 activation and maintenance of urea cycle activity despite the hypometabolic conditions associated with freezing are important aspects in the metabolic survival strategies of the wood frog.",
"genre": "research_article",
"id": "sg:pub.10.1007/s11010-018-3468-8",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1016672",
"issn": [
"0300-8177",
"1573-4919"
],
"name": "Molecular and Cellular Biochemistry",
"type": "Periodical"
},
{
"issueNumber": "1-2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "455"
}
],
"name": "Purification of carbamoyl phosphate synthetase 1 (CPS1) from wood frog (Rana sylvatica) liver and its regulation in response to ice-nucleation and subsequent whole-body freezing",
"pagination": "29-39",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11010-018-3468-8"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"1f974ab4e04250bd3690ed4abee11a625540e55fe64606a02709d908f9d57121"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1109816459"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0364456"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30421312"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11010-018-3468-8",
"https://app.dimensions.ai/details/publication/pub.1109816459"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T09:11",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56158_00000005.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1007%2Fs11010-018-3468-8"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11010-018-3468-8'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11010-018-3468-8'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11010-018-3468-8'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11010-018-3468-8'
This table displays all metadata directly associated to this object as RDF triples.
208 TRIPLES
21 PREDICATES
72 URIs
21 LITERALS
9 BLANK NODES