Molecular effect of human umbilical cord blood CD34-positive and CD34-negative stem cells and their conjugate in azoospermic mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-04

AUTHORS

Somia H. Abd Allah, Heba F. Pasha, Abeer A. Abdelrahman, Nehad F. Mazen

ABSTRACT

Currently, azoospermia is one of the most common diseases of male infertility. Stem cell research is the new hope for novel therapy with a higher degree of safety and lower cost. This study aimed to investigate the effect of umbilical cord blood-derived stem cells (" and mesenchymal "UCB-MSCs") and mono-cell layer implanted into the induced azoospermic mice testis. Stem cells were isolated from umbilical cord blood and CD34+ve cells were separated from negative one by Mini MACs column. At 5th week after single injection of busulfan, stained mesenchymal (CD34-ve), hematopoietic stem cells (CD34+ve) and their conjugate (mono-cell layer) were injected locally into testis. At the end of the study, MSCs group showed that mRNA levels of genes related to meiosis (Vasa, SCP3, and PgK2) were increased with significant decrease of FSH and LH levels, compared to control group. Histologically, most of the tubules restored normal architecture. In contrast, HSCs and mono-cell layer groups showed statically insignificant change of FSH, LH, and gene expression, compared to control group. Histologically, distorted seminiferous tubules, with reduction in sperm content, and interstitial mononuclear cellular infiltration were seen. There was significant increase in the optical density of PCNA immune reaction in MSCs group than azoospermia, HSCs, and mono-cell layer, while there was non-significant difference between MSCs and control group. The present study suggested that injection of MSCs into chemotherapeutic-induced azoospermia in mice improved testicular failure; histologically and functionally, by restoration of spermatogenic gene expression while HSC and mono-cell layer showed no effect on spermatogenesis added to that mono-cell layer may induce testicular tissue damage. More... »

PAGES

179-191

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11010-016-2928-2

DOI

http://dx.doi.org/10.1007/s11010-016-2928-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1074187356

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28120211


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD34", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Azoospermia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cord Blood Stem Cell Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fetal Blood", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Follicle Stimulating Hormone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Luteinizing Hormone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Meiosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Zagazig University", 
          "id": "https://www.grid.ac/institutes/grid.31451.32", 
          "name": [
            "Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, 2345, Zagazig, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Abd Allah", 
        "givenName": "Somia H.", 
        "id": "sg:person.016375745677.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016375745677.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zagazig University", 
          "id": "https://www.grid.ac/institutes/grid.31451.32", 
          "name": [
            "Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, 2345, Zagazig, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pasha", 
        "givenName": "Heba F.", 
        "id": "sg:person.01336514100.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336514100.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zagazig University", 
          "id": "https://www.grid.ac/institutes/grid.31451.32", 
          "name": [
            "Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, 2345, Zagazig, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Abdelrahman", 
        "givenName": "Abeer A.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Zagazig University", 
          "id": "https://www.grid.ac/institutes/grid.31451.32", 
          "name": [
            "Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mazen", 
        "givenName": "Nehad F.", 
        "id": "sg:person.01363737574.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363737574.56"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1046/j.1365-2141.2001.03044.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002689808"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mefs.2012.06.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003565885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004396358", 
          "https://doi.org/10.1038/nbt1047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004396358", 
          "https://doi.org/10.1038/nbt1047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2006.10.170", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005982888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2353/ajpath.2007.060543", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010046393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40001-014-0080-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010287226", 
          "https://doi.org/10.1186/s40001-014-0080-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40001-014-0080-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010287226", 
          "https://doi.org/10.1186/s40001-014-0080-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014439735", 
          "https://doi.org/10.1038/nature03206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014439735", 
          "https://doi.org/10.1038/nature03206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/10520290802521804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015224952"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0002-9378(64)90704-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021226216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.yexcr.2011.03.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022175365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4103/1008-682x.129209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028309498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2013/529589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029369683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.fertnstert.2008.09.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029886605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10616-014-9718-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032575778", 
          "https://doi.org/10.1007/s10616-014-9718-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0303-7207(99)00234-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032621393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0890-6238(01)00182-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033920155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0378-1119(98)00420-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035683045"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s000180050363", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038816052", 
          "https://doi.org/10.1007/s000180050363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2012.10.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039705233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrneurol.2010.35", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041744466", 
          "https://doi.org/10.1038/nrneurol.2010.35"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrneurol.2010.35", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041744466", 
          "https://doi.org/10.1038/nrneurol.2010.35"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biocel.2003.10.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042127018"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3700429", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045322386", 
          "https://doi.org/10.1038/labinvest.3700429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3700429", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045322386", 
          "https://doi.org/10.1038/labinvest.3700429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/labinvest.3700429", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045322386", 
          "https://doi.org/10.1038/labinvest.3700429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1939-4640.1997.tb01879.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045559597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/icb.2012.64", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047967149", 
          "https://doi.org/10.1038/icb.2012.64"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2012.07.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049944648"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2217/rme.10.72", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050558691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/ajcp/92.1.62", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053335789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/aja.2010.101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056439647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/aja.2010.101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056439647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/aja.2011.66", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056439961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/clo.2008.0004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059244982"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/humrep/dev301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059648655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1160/th11-08-0596", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063292839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/en.2006-0162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064247957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077415790", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077431102", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078881480", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082534224", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082889496", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-04", 
    "datePublishedReg": "2017-04-01", 
    "description": "Currently, azoospermia is one of the most common diseases of male infertility. Stem cell research is the new hope for novel therapy with a higher degree of safety and lower cost. This study aimed to investigate the effect of umbilical cord blood-derived stem cells (\" and mesenchymal \"UCB-MSCs\") and mono-cell layer implanted into the induced azoospermic mice testis. Stem cells were isolated from umbilical cord blood and CD34+ve cells were separated from negative one by Mini MACs column. At 5th week after single injection of busulfan, stained mesenchymal (CD34-ve), hematopoietic stem cells (CD34+ve) and their conjugate (mono-cell layer) were injected locally into testis. At the end of the study, MSCs group showed that mRNA levels of genes related to meiosis (Vasa, SCP3, and PgK2) were increased with significant decrease of FSH and LH levels, compared to control group. Histologically, most of the tubules restored normal architecture. In contrast, HSCs and mono-cell layer groups showed statically insignificant change of FSH, LH, and gene expression, compared to control group. Histologically, distorted seminiferous tubules, with reduction in sperm content, and interstitial mononuclear cellular infiltration were seen. There was significant increase in the optical density of PCNA immune reaction in MSCs group than azoospermia, HSCs, and mono-cell layer, while there was non-significant difference between MSCs and control group. The present study suggested that injection of MSCs into chemotherapeutic-induced azoospermia in mice improved testicular failure; histologically and functionally, by restoration of spermatogenic gene expression while HSC and mono-cell layer showed no effect on spermatogenesis added to that mono-cell layer may induce testicular tissue damage.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11010-016-2928-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1016672", 
        "issn": [
          "0300-8177", 
          "1573-4919"
        ], 
        "name": "Molecular and Cellular Biochemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "428"
      }
    ], 
    "name": "Molecular effect of human umbilical cord blood CD34-positive and CD34-negative stem cells and their conjugate in azoospermic mice", 
    "pagination": "179-191", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "088dd00f9bd082ac64c41db0c0c8dfb6d9bc072dbd4fa3acd042a31cda884ac4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28120211"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0364456"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11010-016-2928-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1074187356"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11010-016-2928-2", 
      "https://app.dimensions.ai/details/publication/pub.1074187356"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000347_0000000347/records_89790_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs11010-016-2928-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11010-016-2928-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11010-016-2928-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11010-016-2928-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11010-016-2928-2'


 

This table displays all metadata directly associated to this object as RDF triples.

259 TRIPLES      21 PREDICATES      80 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11010-016-2928-2 schema:about N350bffbb784a46bb832da157b62b9731
2 N63b7dee36de64103b17de6d9bb768c92
3 N73535e039ddf43de891016216d38bf30
4 N897f0024e62d4c6eb4fa9d500d893e03
5 N9609abf904ba4febadc87b569e1d8745
6 Na7b9291f7b61479391a9814f2106aff2
7 Nb07a7584dec44dcfab367243b9dbb40c
8 Nb3412eee988c42638028109db908a353
9 Nb4e7f949e759412ba0fddd6b83f52eb7
10 Nc410063e93cb4977a6cc00f191a13271
11 Nd3b02266848243c284556bd8a607c831
12 Ndbefd5c228e647f1a06faeb78676b592
13 Nff9549ea1c91467691194589798c53cb
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N724f3d34966146138061c145a009f05e
17 schema:citation sg:pub.10.1007/s000180050363
18 sg:pub.10.1007/s10616-014-9718-z
19 sg:pub.10.1038/icb.2012.64
20 sg:pub.10.1038/labinvest.3700429
21 sg:pub.10.1038/nature03206
22 sg:pub.10.1038/nbt1047
23 sg:pub.10.1038/nrneurol.2010.35
24 sg:pub.10.1186/s40001-014-0080-6
25 https://app.dimensions.ai/details/publication/pub.1077415790
26 https://app.dimensions.ai/details/publication/pub.1077431102
27 https://app.dimensions.ai/details/publication/pub.1078881480
28 https://app.dimensions.ai/details/publication/pub.1082534224
29 https://app.dimensions.ai/details/publication/pub.1082889496
30 https://doi.org/10.1002/j.1939-4640.1997.tb01879.x
31 https://doi.org/10.1016/0002-9378(64)90704-5
32 https://doi.org/10.1016/j.bbadis.2012.10.004
33 https://doi.org/10.1016/j.bbrc.2006.10.170
34 https://doi.org/10.1016/j.biocel.2003.10.015
35 https://doi.org/10.1016/j.celrep.2012.07.015
36 https://doi.org/10.1016/j.fertnstert.2008.09.036
37 https://doi.org/10.1016/j.mefs.2012.06.001
38 https://doi.org/10.1016/j.yexcr.2011.03.013
39 https://doi.org/10.1016/s0303-7207(99)00234-8
40 https://doi.org/10.1016/s0378-1119(98)00420-x
41 https://doi.org/10.1016/s0890-6238(01)00182-4
42 https://doi.org/10.1038/aja.2010.101
43 https://doi.org/10.1038/aja.2011.66
44 https://doi.org/10.1046/j.1365-2141.2001.03044.x
45 https://doi.org/10.1080/10520290802521804
46 https://doi.org/10.1089/clo.2008.0004
47 https://doi.org/10.1093/ajcp/92.1.62
48 https://doi.org/10.1093/humrep/dev301
49 https://doi.org/10.1155/2013/529589
50 https://doi.org/10.1160/th11-08-0596
51 https://doi.org/10.1210/en.2006-0162
52 https://doi.org/10.2217/rme.10.72
53 https://doi.org/10.2353/ajpath.2007.060543
54 https://doi.org/10.4103/1008-682x.129209
55 schema:datePublished 2017-04
56 schema:datePublishedReg 2017-04-01
57 schema:description Currently, azoospermia is one of the most common diseases of male infertility. Stem cell research is the new hope for novel therapy with a higher degree of safety and lower cost. This study aimed to investigate the effect of umbilical cord blood-derived stem cells (" and mesenchymal "UCB-MSCs") and mono-cell layer implanted into the induced azoospermic mice testis. Stem cells were isolated from umbilical cord blood and CD34+ve cells were separated from negative one by Mini MACs column. At 5th week after single injection of busulfan, stained mesenchymal (CD34-ve), hematopoietic stem cells (CD34+ve) and their conjugate (mono-cell layer) were injected locally into testis. At the end of the study, MSCs group showed that mRNA levels of genes related to meiosis (Vasa, SCP3, and PgK2) were increased with significant decrease of FSH and LH levels, compared to control group. Histologically, most of the tubules restored normal architecture. In contrast, HSCs and mono-cell layer groups showed statically insignificant change of FSH, LH, and gene expression, compared to control group. Histologically, distorted seminiferous tubules, with reduction in sperm content, and interstitial mononuclear cellular infiltration were seen. There was significant increase in the optical density of PCNA immune reaction in MSCs group than azoospermia, HSCs, and mono-cell layer, while there was non-significant difference between MSCs and control group. The present study suggested that injection of MSCs into chemotherapeutic-induced azoospermia in mice improved testicular failure; histologically and functionally, by restoration of spermatogenic gene expression while HSC and mono-cell layer showed no effect on spermatogenesis added to that mono-cell layer may induce testicular tissue damage.
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf N3785dd327811464999c700e5392219c0
62 N5aaf7043653d463bbf4c025b033da3be
63 sg:journal.1016672
64 schema:name Molecular effect of human umbilical cord blood CD34-positive and CD34-negative stem cells and their conjugate in azoospermic mice
65 schema:pagination 179-191
66 schema:productId N1fb5b1fc117d4b988a498e4343ed0b08
67 N380beba1453b4c0790411450d2613ada
68 N8b26b6dd55a045c6a908ba8d92049d86
69 N950573303559427a8f1e5499baab895f
70 Nd6e88cf5bf864e7facfa106df1b29862
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074187356
72 https://doi.org/10.1007/s11010-016-2928-2
73 schema:sdDatePublished 2019-04-11T09:52
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher Ne07f9e27236a42f7b830d6025a14b6b8
76 schema:url https://link.springer.com/10.1007%2Fs11010-016-2928-2
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N1fb5b1fc117d4b988a498e4343ed0b08 schema:name doi
81 schema:value 10.1007/s11010-016-2928-2
82 rdf:type schema:PropertyValue
83 N350bffbb784a46bb832da157b62b9731 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Gene Expression Regulation
85 rdf:type schema:DefinedTerm
86 N3785dd327811464999c700e5392219c0 schema:volumeNumber 428
87 rdf:type schema:PublicationVolume
88 N380beba1453b4c0790411450d2613ada schema:name dimensions_id
89 schema:value pub.1074187356
90 rdf:type schema:PropertyValue
91 N5aaf7043653d463bbf4c025b033da3be schema:issueNumber 1-2
92 rdf:type schema:PublicationIssue
93 N63b7dee36de64103b17de6d9bb768c92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Male
95 rdf:type schema:DefinedTerm
96 N6d1f1b378b2c4445aa62be711ad22a58 rdf:first Ne66aa0d4a4a845ce953885c76b398d11
97 rdf:rest N7c1e6f924e754b81bf989c13bdf1ba64
98 N724f3d34966146138061c145a009f05e rdf:first sg:person.016375745677.00
99 rdf:rest Nf668844d448f42e1a03aea3061e44d34
100 N73535e039ddf43de891016216d38bf30 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Follicle Stimulating Hormone
102 rdf:type schema:DefinedTerm
103 N7c1e6f924e754b81bf989c13bdf1ba64 rdf:first sg:person.01363737574.56
104 rdf:rest rdf:nil
105 N897f0024e62d4c6eb4fa9d500d893e03 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Disease Models, Animal
107 rdf:type schema:DefinedTerm
108 N8b26b6dd55a045c6a908ba8d92049d86 schema:name nlm_unique_id
109 schema:value 0364456
110 rdf:type schema:PropertyValue
111 N950573303559427a8f1e5499baab895f schema:name readcube_id
112 schema:value 088dd00f9bd082ac64c41db0c0c8dfb6d9bc072dbd4fa3acd042a31cda884ac4
113 rdf:type schema:PropertyValue
114 N9609abf904ba4febadc87b569e1d8745 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Azoospermia
116 rdf:type schema:DefinedTerm
117 Na7b9291f7b61479391a9814f2106aff2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Meiosis
119 rdf:type schema:DefinedTerm
120 Nb07a7584dec44dcfab367243b9dbb40c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Animals
122 rdf:type schema:DefinedTerm
123 Nb3412eee988c42638028109db908a353 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Luteinizing Hormone
125 rdf:type schema:DefinedTerm
126 Nb4e7f949e759412ba0fddd6b83f52eb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Humans
128 rdf:type schema:DefinedTerm
129 Nc410063e93cb4977a6cc00f191a13271 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Cord Blood Stem Cell Transplantation
131 rdf:type schema:DefinedTerm
132 Nd3b02266848243c284556bd8a607c831 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Fetal Blood
134 rdf:type schema:DefinedTerm
135 Nd6e88cf5bf864e7facfa106df1b29862 schema:name pubmed_id
136 schema:value 28120211
137 rdf:type schema:PropertyValue
138 Ndbefd5c228e647f1a06faeb78676b592 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Mice
140 rdf:type schema:DefinedTerm
141 Ne07f9e27236a42f7b830d6025a14b6b8 schema:name Springer Nature - SN SciGraph project
142 rdf:type schema:Organization
143 Ne66aa0d4a4a845ce953885c76b398d11 schema:affiliation https://www.grid.ac/institutes/grid.31451.32
144 schema:familyName Abdelrahman
145 schema:givenName Abeer A.
146 rdf:type schema:Person
147 Nf668844d448f42e1a03aea3061e44d34 rdf:first sg:person.01336514100.16
148 rdf:rest N6d1f1b378b2c4445aa62be711ad22a58
149 Nff9549ea1c91467691194589798c53cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Antigens, CD34
151 rdf:type schema:DefinedTerm
152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biological Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
156 schema:name Genetics
157 rdf:type schema:DefinedTerm
158 sg:journal.1016672 schema:issn 0300-8177
159 1573-4919
160 schema:name Molecular and Cellular Biochemistry
161 rdf:type schema:Periodical
162 sg:person.01336514100.16 schema:affiliation https://www.grid.ac/institutes/grid.31451.32
163 schema:familyName Pasha
164 schema:givenName Heba F.
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01336514100.16
166 rdf:type schema:Person
167 sg:person.01363737574.56 schema:affiliation https://www.grid.ac/institutes/grid.31451.32
168 schema:familyName Mazen
169 schema:givenName Nehad F.
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363737574.56
171 rdf:type schema:Person
172 sg:person.016375745677.00 schema:affiliation https://www.grid.ac/institutes/grid.31451.32
173 schema:familyName Abd Allah
174 schema:givenName Somia H.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016375745677.00
176 rdf:type schema:Person
177 sg:pub.10.1007/s000180050363 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038816052
178 https://doi.org/10.1007/s000180050363
179 rdf:type schema:CreativeWork
180 sg:pub.10.1007/s10616-014-9718-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1032575778
181 https://doi.org/10.1007/s10616-014-9718-z
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/icb.2012.64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047967149
184 https://doi.org/10.1038/icb.2012.64
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/labinvest.3700429 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045322386
187 https://doi.org/10.1038/labinvest.3700429
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/nature03206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014439735
190 https://doi.org/10.1038/nature03206
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nbt1047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004396358
193 https://doi.org/10.1038/nbt1047
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/nrneurol.2010.35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041744466
196 https://doi.org/10.1038/nrneurol.2010.35
197 rdf:type schema:CreativeWork
198 sg:pub.10.1186/s40001-014-0080-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010287226
199 https://doi.org/10.1186/s40001-014-0080-6
200 rdf:type schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1077415790 schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1077431102 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1078881480 schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1082534224 schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1082889496 schema:CreativeWork
206 https://doi.org/10.1002/j.1939-4640.1997.tb01879.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045559597
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0002-9378(64)90704-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021226216
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.bbadis.2012.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039705233
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.bbrc.2006.10.170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005982888
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/j.biocel.2003.10.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042127018
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/j.celrep.2012.07.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049944648
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.fertnstert.2008.09.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029886605
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.mefs.2012.06.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003565885
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.yexcr.2011.03.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022175365
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/s0303-7207(99)00234-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032621393
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/s0378-1119(98)00420-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035683045
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/s0890-6238(01)00182-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033920155
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1038/aja.2010.101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056439647
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1038/aja.2011.66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056439961
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1046/j.1365-2141.2001.03044.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002689808
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1080/10520290802521804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015224952
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1089/clo.2008.0004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059244982
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1093/ajcp/92.1.62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053335789
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1093/humrep/dev301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059648655
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1155/2013/529589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029369683
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1160/th11-08-0596 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063292839
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1210/en.2006-0162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064247957
249 rdf:type schema:CreativeWork
250 https://doi.org/10.2217/rme.10.72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050558691
251 rdf:type schema:CreativeWork
252 https://doi.org/10.2353/ajpath.2007.060543 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010046393
253 rdf:type schema:CreativeWork
254 https://doi.org/10.4103/1008-682x.129209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028309498
255 rdf:type schema:CreativeWork
256 https://www.grid.ac/institutes/grid.31451.32 schema:alternateName Zagazig University
257 schema:name Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
258 Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, 2345, Zagazig, Egypt
259 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...