Effect of the redox state on HIV-1 tat protein multimerization and cell internalization and trafficking View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-12

AUTHORS

Raffaella Pierleoni, Michele Menotta, Antonella Antonelli, Carla Sfara, Giordano Serafini, Sabrina Dominici, Maria Elena Laguardia, Annalisa Salis, Gianluca Damonte, Lucia Banci, Marco Porcu, Paolo Monini, Barbara Ensoli, Mauro Magnani

ABSTRACT

The redox state of the cysteine-rich region of the HIV Tat protein is known to play a crucial role in Tat biological activity. In this article, we show that Tat displays two alternative functional states depending on the presence of either one or three reduced sulphydryl groups in the cysteine-rich region, respectively. Using different approaches, a disulfide pattern has been defined for the Tat protein and a specific DTT-dependent breaking order of disulfide bonds highlighted. The Tat redox state deeply influences macrophage protein uptake. Immunoistochemistry analysis shows that the oxidized protein does not enter cells, whereas partially reduced protein reaches the cytosol and, to a limited extent, the nucleus. Finally electrophoretic analysis shows Tat high-molecular weight multi-aggregation, resulting in the loss of biological activity. This is due to strong electrostatic and metal-binding interactions, whereas Tat dimerization involves metal-binding interactions as well as disulfide bond formation. More... »

PAGES

105-118

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11010-010-0564-9

DOI

http://dx.doi.org/10.1007/s11010-010-0564-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017534425

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20721684


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, High Pressure Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cysteine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disulfides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endocytosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Products, tat", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macrophages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mass Spectrometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidation-Reduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Multimerization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Transport", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pierleoni", 
        "givenName": "Raffaella", 
        "id": "sg:person.01022453236.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022453236.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Menotta", 
        "givenName": "Michele", 
        "id": "sg:person.01125765675.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125765675.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Antonelli", 
        "givenName": "Antonella", 
        "id": "sg:person.01251766365.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251766365.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sfara", 
        "givenName": "Carla", 
        "id": "sg:person.01221623013.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221623013.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Serafini", 
        "givenName": "Giordano", 
        "id": "sg:person.01123742735.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123742735.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dominici", 
        "givenName": "Sabrina", 
        "id": "sg:person.01210363450.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210363450.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Diatheva srl, Via Piceno 137/F, 61032, Fano, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Laguardia", 
        "givenName": "Maria Elena", 
        "id": "sg:person.01265757041.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265757041.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Genoa", 
          "id": "https://www.grid.ac/institutes/grid.5606.5", 
          "name": [
            "Department of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 7, Genova, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Salis", 
        "givenName": "Annalisa", 
        "id": "sg:person.0732530052.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732530052.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Genoa", 
          "id": "https://www.grid.ac/institutes/grid.5606.5", 
          "name": [
            "Department of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 7, Genova, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Damonte", 
        "givenName": "Gianluca", 
        "id": "sg:person.01021526766.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021526766.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florence", 
          "id": "https://www.grid.ac/institutes/grid.8404.8", 
          "name": [
            "Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino Florence, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Banci", 
        "givenName": "Lucia", 
        "id": "sg:person.01242460216.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460216.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Florence", 
          "id": "https://www.grid.ac/institutes/grid.8404.8", 
          "name": [
            "Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino Florence, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Porcu", 
        "givenName": "Marco", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Centro Nazionale AIDS, Istituto Superiore di Sanit\u00e0, Viale Regina Elena, 299, 00161, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Monini", 
        "givenName": "Paolo", 
        "id": "sg:person.0626770720.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626770720.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Superiore di Sanit\u00e0", 
          "id": "https://www.grid.ac/institutes/grid.416651.1", 
          "name": [
            "Centro Nazionale AIDS, Istituto Superiore di Sanit\u00e0, Viale Regina Elena, 299, 00161, Rome, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ensoli", 
        "givenName": "Barbara", 
        "id": "sg:person.0764161775.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764161775.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Urbino", 
          "id": "https://www.grid.ac/institutes/grid.12711.34", 
          "name": [
            "Department of Molecular Sciences, University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Magnani", 
        "givenName": "Mauro", 
        "id": "sg:person.0752252542.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752252542.98"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/1097-0134(20001115)41:3<415::aid-prot130>3.0.co;2-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000807177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.4210102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000906630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0020333", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004041016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02253380", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008825393", 
          "https://doi.org/10.1007/bf02253380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02253380", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008825393", 
          "https://doi.org/10.1007/bf02253380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1742-4690-3-53", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012792981", 
          "https://doi.org/10.1186/1742-4690-3-53"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1996.1554", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013949272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/345084a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014935163", 
          "https://doi.org/10.1038/345084a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2009.01.090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015668983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0264-410x(00)00393-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016551422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m509899200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017257768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(91)90797-f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018828895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.79.1.175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021299738"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(00)00109-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023017055"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/320367a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024975907", 
          "https://doi.org/10.1038/320367a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/428909", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028529523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m510748200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030934923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.0711406", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032416900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/9488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035551657", 
          "https://doi.org/10.1038/9488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/9488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035551657", 
          "https://doi.org/10.1038/9488"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.17.7941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036554883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199712000-00006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037096984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002030-199712000-00006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037096984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ana.21292", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038064549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2005.01.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040199284"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkg519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040615905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040895886", 
          "https://doi.org/10.1038/nature09131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040895886", 
          "https://doi.org/10.1038/nature09131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.41.28837", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044231472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(72)25041-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047548445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0140-6736(90)90001-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047687737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0140-6736(90)90001-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047687737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.5.3157-3166.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048595361"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/qad.0b013e32831392d4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048879950"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/qad.0b013e32831392d4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048879950"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042560-199512000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050433277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042560-199512000-00003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050433277"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/qad.0b013e3280112cd1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052284168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/qad.0b013e3280112cd1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052284168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biocel.2004.02.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053535623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0270034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055196522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0270034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055196522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/104454902760330138", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059203768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2832944", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062564672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2990040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062580015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.168.1.197", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074964103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075533286", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076417101", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/aid.1990.6.1257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078210218"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078996369", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082666101", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082797902", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-12", 
    "datePublishedReg": "2010-12-01", 
    "description": "The redox state of the cysteine-rich region of the HIV Tat protein is known to play a crucial role in Tat biological activity. In this article, we show that Tat displays two alternative functional states depending on the presence of either one or three reduced sulphydryl groups in the cysteine-rich region, respectively. Using different approaches, a disulfide pattern has been defined for the Tat protein and a specific DTT-dependent breaking order of disulfide bonds highlighted. The Tat redox state deeply influences macrophage protein uptake. Immunoistochemistry analysis shows that the oxidized protein does not enter cells, whereas partially reduced protein reaches the cytosol and, to a limited extent, the nucleus. Finally electrophoretic analysis shows Tat high-molecular weight multi-aggregation, resulting in the loss of biological activity. This is due to strong electrostatic and metal-binding interactions, whereas Tat dimerization involves metal-binding interactions as well as disulfide bond formation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11010-010-0564-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1016672", 
        "issn": [
          "0300-8177", 
          "1573-4919"
        ], 
        "name": "Molecular and Cellular Biochemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "345"
      }
    ], 
    "name": "Effect of the redox state on HIV-1 tat protein multimerization and cell internalization and trafficking", 
    "pagination": "105-118", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "538a8eaf91d12953f4d5504779c9ffa7e12f2dd297c307dc668d10405034458a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20721684"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0364456"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11010-010-0564-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017534425"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11010-010-0564-9", 
      "https://app.dimensions.ai/details/publication/pub.1017534425"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000350_0000000350/records_77577_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11010-010-0564-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11010-010-0564-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11010-010-0564-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11010-010-0564-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11010-010-0564-9'


 

This table displays all metadata directly associated to this object as RDF triples.

356 TRIPLES      21 PREDICATES      86 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11010-010-0564-9 schema:about N1962555ae5f74c388f6ff7449bd00e90
2 N450cb414c37a4ebfa2dbc38857125f14
3 N4a97124af6f04fd49221d22b072b483e
4 N59fa41d6865f41e8a14d0910fc285355
5 N87c4f3774f3f4674b64063b0f8b7b0e8
6 N94c6aae270a24f49af367f4fac411814
7 N98d789c7db64405e8e4082af59459f1e
8 N9fde3b2ccfba4fc29163a214fc6b9f74
9 Nadc91ea2e4794c1ebabbbfc15a95eb5b
10 Nba5533283a2543058a63e6c2ea27f229
11 Nbe2dab2feb02406c965f0c8045e5883e
12 Nd8e62b4c036d44ccabb2450cc3001e21
13 Neb1581fdda1d4fe4b49347f7f68416da
14 anzsrc-for:06
15 anzsrc-for:0601
16 schema:author Nd3240b6194d948bb92c5f6eeced5db5d
17 schema:citation sg:pub.10.1007/bf02253380
18 sg:pub.10.1038/227680a0
19 sg:pub.10.1038/320367a0
20 sg:pub.10.1038/345084a0
21 sg:pub.10.1038/9488
22 sg:pub.10.1038/nature09131
23 sg:pub.10.1186/1742-4690-3-53
24 https://app.dimensions.ai/details/publication/pub.1075533286
25 https://app.dimensions.ai/details/publication/pub.1076417101
26 https://app.dimensions.ai/details/publication/pub.1078996369
27 https://app.dimensions.ai/details/publication/pub.1082666101
28 https://app.dimensions.ai/details/publication/pub.1082797902
29 https://doi.org/10.1002/1097-0134(20001115)41:3<415::aid-prot130>3.0.co;2-7
30 https://doi.org/10.1002/ana.21292
31 https://doi.org/10.1006/bbrc.1996.1554
32 https://doi.org/10.1016/0042-6822(91)90797-f
33 https://doi.org/10.1016/0140-6736(90)90001-l
34 https://doi.org/10.1016/j.biocel.2004.02.018
35 https://doi.org/10.1016/j.sbi.2005.01.002
36 https://doi.org/10.1016/j.vaccine.2009.01.090
37 https://doi.org/10.1016/s0076-6879(72)25041-8
38 https://doi.org/10.1016/s0264-410x(00)00393-5
39 https://doi.org/10.1016/s0955-0674(00)00109-5
40 https://doi.org/10.1021/bi0270034
41 https://doi.org/10.1073/pnas.79.1.175
42 https://doi.org/10.1073/pnas.90.17.7941
43 https://doi.org/10.1074/jbc.274.41.28837
44 https://doi.org/10.1074/jbc.m509899200
45 https://doi.org/10.1074/jbc.m510748200
46 https://doi.org/10.1086/428909
47 https://doi.org/10.1089/104454902760330138
48 https://doi.org/10.1089/aid.1990.6.1257
49 https://doi.org/10.1093/nar/gkg519
50 https://doi.org/10.1097/00002030-199712000-00006
51 https://doi.org/10.1097/00042560-199512000-00003
52 https://doi.org/10.1097/qad.0b013e3280112cd1
53 https://doi.org/10.1097/qad.0b013e32831392d4
54 https://doi.org/10.1110/ps.4210102
55 https://doi.org/10.1126/science.2832944
56 https://doi.org/10.1126/science.2990040
57 https://doi.org/10.1128/jvi.77.5.3157-3166.2003
58 https://doi.org/10.1371/journal.pbio.0020333
59 https://doi.org/10.4049/jimmunol.0711406
60 https://doi.org/10.4049/jimmunol.168.1.197
61 schema:datePublished 2010-12
62 schema:datePublishedReg 2010-12-01
63 schema:description The redox state of the cysteine-rich region of the HIV Tat protein is known to play a crucial role in Tat biological activity. In this article, we show that Tat displays two alternative functional states depending on the presence of either one or three reduced sulphydryl groups in the cysteine-rich region, respectively. Using different approaches, a disulfide pattern has been defined for the Tat protein and a specific DTT-dependent breaking order of disulfide bonds highlighted. The Tat redox state deeply influences macrophage protein uptake. Immunoistochemistry analysis shows that the oxidized protein does not enter cells, whereas partially reduced protein reaches the cytosol and, to a limited extent, the nucleus. Finally electrophoretic analysis shows Tat high-molecular weight multi-aggregation, resulting in the loss of biological activity. This is due to strong electrostatic and metal-binding interactions, whereas Tat dimerization involves metal-binding interactions as well as disulfide bond formation.
64 schema:genre research_article
65 schema:inLanguage en
66 schema:isAccessibleForFree false
67 schema:isPartOf N7f4a994a8917433b9bf4bb40118d1784
68 N8191eee70969459791393baeb5989888
69 sg:journal.1016672
70 schema:name Effect of the redox state on HIV-1 tat protein multimerization and cell internalization and trafficking
71 schema:pagination 105-118
72 schema:productId N0f238bced3c546efa21da0feeb61a622
73 N7f97a6f95e254aca8f82f33c0c1b0f77
74 Nb879f0bd44af4b019eb6a0cc8beb90dc
75 Nbac5c2c091d846fdb343c1d97d13be0a
76 Nf052d22dafc84dac9a597da465bb5c45
77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017534425
78 https://doi.org/10.1007/s11010-010-0564-9
79 schema:sdDatePublished 2019-04-11T10:50
80 schema:sdLicense https://scigraph.springernature.com/explorer/license/
81 schema:sdPublisher N02e95e0d99d64c88879e3f97116c85ae
82 schema:url http://link.springer.com/10.1007%2Fs11010-010-0564-9
83 sgo:license sg:explorer/license/
84 sgo:sdDataset articles
85 rdf:type schema:ScholarlyArticle
86 N02e95e0d99d64c88879e3f97116c85ae schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N093c23b9ef1b4dacac79e01a8221dc58 rdf:first sg:person.01123742735.46
89 rdf:rest N2b4f136797494022839c434632dc5dba
90 N0f238bced3c546efa21da0feeb61a622 schema:name doi
91 schema:value 10.1007/s11010-010-0564-9
92 rdf:type schema:PropertyValue
93 N14aee84506084f789c5671e7a85154c4 rdf:first sg:person.01265757041.44
94 rdf:rest N72cde11303754f509d7ea154c614cc27
95 N1962555ae5f74c388f6ff7449bd00e90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Disulfides
97 rdf:type schema:DefinedTerm
98 N1ec5cfb120ce4e64b8c4f7740df0b4bf rdf:first sg:person.0626770720.54
99 rdf:rest N4f17ae23c168456ca42b6fe088376934
100 N215ce791f8bf45b19c7e22bc902684a6 rdf:first sg:person.01242460216.00
101 rdf:rest N626c4f4a4cf04e1586d0fcc9c1c76a78
102 N2b4f136797494022839c434632dc5dba rdf:first sg:person.01210363450.49
103 rdf:rest N14aee84506084f789c5671e7a85154c4
104 N450cb414c37a4ebfa2dbc38857125f14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Humans
106 rdf:type schema:DefinedTerm
107 N4a97124af6f04fd49221d22b072b483e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Cells, Cultured
109 rdf:type schema:DefinedTerm
110 N4f17ae23c168456ca42b6fe088376934 rdf:first sg:person.0764161775.02
111 rdf:rest N64fda75eb9004e948fd4c19301602178
112 N59fa41d6865f41e8a14d0910fc285355 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Mass Spectrometry
114 rdf:type schema:DefinedTerm
115 N5aba12c1e869465bb7a2b6ea3f1c4065 rdf:first sg:person.01221623013.13
116 rdf:rest N093c23b9ef1b4dacac79e01a8221dc58
117 N61fbf34ec2ee4acf837cb65fb4d4cf6b rdf:first sg:person.01251766365.09
118 rdf:rest N5aba12c1e869465bb7a2b6ea3f1c4065
119 N626c4f4a4cf04e1586d0fcc9c1c76a78 rdf:first Ned5d26c64b694288a4223b8348555c27
120 rdf:rest N1ec5cfb120ce4e64b8c4f7740df0b4bf
121 N64fda75eb9004e948fd4c19301602178 rdf:first sg:person.0752252542.98
122 rdf:rest rdf:nil
123 N72cde11303754f509d7ea154c614cc27 rdf:first sg:person.0732530052.09
124 rdf:rest Nc73fbe0537bb44808338c5c8c91cd883
125 N7f4a994a8917433b9bf4bb40118d1784 schema:volumeNumber 345
126 rdf:type schema:PublicationVolume
127 N7f97a6f95e254aca8f82f33c0c1b0f77 schema:name pubmed_id
128 schema:value 20721684
129 rdf:type schema:PropertyValue
130 N8191eee70969459791393baeb5989888 schema:issueNumber 1-2
131 rdf:type schema:PublicationIssue
132 N87c4f3774f3f4674b64063b0f8b7b0e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Models, Molecular
134 rdf:type schema:DefinedTerm
135 N94c6aae270a24f49af367f4fac411814 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Endocytosis
137 rdf:type schema:DefinedTerm
138 N98d789c7db64405e8e4082af59459f1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Chromatography, High Pressure Liquid
140 rdf:type schema:DefinedTerm
141 N9fde3b2ccfba4fc29163a214fc6b9f74 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Cysteine
143 rdf:type schema:DefinedTerm
144 Nadc91ea2e4794c1ebabbbfc15a95eb5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Oxidation-Reduction
146 rdf:type schema:DefinedTerm
147 Nb879f0bd44af4b019eb6a0cc8beb90dc schema:name nlm_unique_id
148 schema:value 0364456
149 rdf:type schema:PropertyValue
150 Nb8fb5e2cfd1f4316a50d091cbeab914a rdf:first sg:person.01125765675.00
151 rdf:rest N61fbf34ec2ee4acf837cb65fb4d4cf6b
152 Nba5533283a2543058a63e6c2ea27f229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Protein Multimerization
154 rdf:type schema:DefinedTerm
155 Nbac5c2c091d846fdb343c1d97d13be0a schema:name dimensions_id
156 schema:value pub.1017534425
157 rdf:type schema:PropertyValue
158 Nbe2dab2feb02406c965f0c8045e5883e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Gene Products, tat
160 rdf:type schema:DefinedTerm
161 Nc73fbe0537bb44808338c5c8c91cd883 rdf:first sg:person.01021526766.53
162 rdf:rest N215ce791f8bf45b19c7e22bc902684a6
163 Nd3240b6194d948bb92c5f6eeced5db5d rdf:first sg:person.01022453236.20
164 rdf:rest Nb8fb5e2cfd1f4316a50d091cbeab914a
165 Nd8e62b4c036d44ccabb2450cc3001e21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Macrophages
167 rdf:type schema:DefinedTerm
168 Neb1581fdda1d4fe4b49347f7f68416da schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Protein Transport
170 rdf:type schema:DefinedTerm
171 Ned5d26c64b694288a4223b8348555c27 schema:affiliation https://www.grid.ac/institutes/grid.8404.8
172 schema:familyName Porcu
173 schema:givenName Marco
174 rdf:type schema:Person
175 Nf052d22dafc84dac9a597da465bb5c45 schema:name readcube_id
176 schema:value 538a8eaf91d12953f4d5504779c9ffa7e12f2dd297c307dc668d10405034458a
177 rdf:type schema:PropertyValue
178 Nf57d77d3da454c46bd77fd3eff9cc705 schema:name Diatheva srl, Via Piceno 137/F, 61032, Fano, Italy
179 rdf:type schema:Organization
180 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
181 schema:name Biological Sciences
182 rdf:type schema:DefinedTerm
183 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
184 schema:name Biochemistry and Cell Biology
185 rdf:type schema:DefinedTerm
186 sg:journal.1016672 schema:issn 0300-8177
187 1573-4919
188 schema:name Molecular and Cellular Biochemistry
189 rdf:type schema:Periodical
190 sg:person.01021526766.53 schema:affiliation https://www.grid.ac/institutes/grid.5606.5
191 schema:familyName Damonte
192 schema:givenName Gianluca
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021526766.53
194 rdf:type schema:Person
195 sg:person.01022453236.20 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
196 schema:familyName Pierleoni
197 schema:givenName Raffaella
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022453236.20
199 rdf:type schema:Person
200 sg:person.01123742735.46 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
201 schema:familyName Serafini
202 schema:givenName Giordano
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123742735.46
204 rdf:type schema:Person
205 sg:person.01125765675.00 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
206 schema:familyName Menotta
207 schema:givenName Michele
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125765675.00
209 rdf:type schema:Person
210 sg:person.01210363450.49 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
211 schema:familyName Dominici
212 schema:givenName Sabrina
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210363450.49
214 rdf:type schema:Person
215 sg:person.01221623013.13 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
216 schema:familyName Sfara
217 schema:givenName Carla
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221623013.13
219 rdf:type schema:Person
220 sg:person.01242460216.00 schema:affiliation https://www.grid.ac/institutes/grid.8404.8
221 schema:familyName Banci
222 schema:givenName Lucia
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460216.00
224 rdf:type schema:Person
225 sg:person.01251766365.09 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
226 schema:familyName Antonelli
227 schema:givenName Antonella
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01251766365.09
229 rdf:type schema:Person
230 sg:person.01265757041.44 schema:affiliation Nf57d77d3da454c46bd77fd3eff9cc705
231 schema:familyName Laguardia
232 schema:givenName Maria Elena
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265757041.44
234 rdf:type schema:Person
235 sg:person.0626770720.54 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
236 schema:familyName Monini
237 schema:givenName Paolo
238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626770720.54
239 rdf:type schema:Person
240 sg:person.0732530052.09 schema:affiliation https://www.grid.ac/institutes/grid.5606.5
241 schema:familyName Salis
242 schema:givenName Annalisa
243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732530052.09
244 rdf:type schema:Person
245 sg:person.0752252542.98 schema:affiliation https://www.grid.ac/institutes/grid.12711.34
246 schema:familyName Magnani
247 schema:givenName Mauro
248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752252542.98
249 rdf:type schema:Person
250 sg:person.0764161775.02 schema:affiliation https://www.grid.ac/institutes/grid.416651.1
251 schema:familyName Ensoli
252 schema:givenName Barbara
253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764161775.02
254 rdf:type schema:Person
255 sg:pub.10.1007/bf02253380 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008825393
256 https://doi.org/10.1007/bf02253380
257 rdf:type schema:CreativeWork
258 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
259 https://doi.org/10.1038/227680a0
260 rdf:type schema:CreativeWork
261 sg:pub.10.1038/320367a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024975907
262 https://doi.org/10.1038/320367a0
263 rdf:type schema:CreativeWork
264 sg:pub.10.1038/345084a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014935163
265 https://doi.org/10.1038/345084a0
266 rdf:type schema:CreativeWork
267 sg:pub.10.1038/9488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035551657
268 https://doi.org/10.1038/9488
269 rdf:type schema:CreativeWork
270 sg:pub.10.1038/nature09131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040895886
271 https://doi.org/10.1038/nature09131
272 rdf:type schema:CreativeWork
273 sg:pub.10.1186/1742-4690-3-53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012792981
274 https://doi.org/10.1186/1742-4690-3-53
275 rdf:type schema:CreativeWork
276 https://app.dimensions.ai/details/publication/pub.1075533286 schema:CreativeWork
277 https://app.dimensions.ai/details/publication/pub.1076417101 schema:CreativeWork
278 https://app.dimensions.ai/details/publication/pub.1078996369 schema:CreativeWork
279 https://app.dimensions.ai/details/publication/pub.1082666101 schema:CreativeWork
280 https://app.dimensions.ai/details/publication/pub.1082797902 schema:CreativeWork
281 https://doi.org/10.1002/1097-0134(20001115)41:3<415::aid-prot130>3.0.co;2-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000807177
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1002/ana.21292 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038064549
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1006/bbrc.1996.1554 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013949272
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1016/0042-6822(91)90797-f schema:sameAs https://app.dimensions.ai/details/publication/pub.1018828895
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1016/0140-6736(90)90001-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1047687737
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1016/j.biocel.2004.02.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053535623
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1016/j.sbi.2005.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040199284
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1016/j.vaccine.2009.01.090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015668983
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1016/s0076-6879(72)25041-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047548445
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1016/s0264-410x(00)00393-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016551422
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1016/s0955-0674(00)00109-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023017055
302 rdf:type schema:CreativeWork
303 https://doi.org/10.1021/bi0270034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055196522
304 rdf:type schema:CreativeWork
305 https://doi.org/10.1073/pnas.79.1.175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021299738
306 rdf:type schema:CreativeWork
307 https://doi.org/10.1073/pnas.90.17.7941 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036554883
308 rdf:type schema:CreativeWork
309 https://doi.org/10.1074/jbc.274.41.28837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044231472
310 rdf:type schema:CreativeWork
311 https://doi.org/10.1074/jbc.m509899200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017257768
312 rdf:type schema:CreativeWork
313 https://doi.org/10.1074/jbc.m510748200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030934923
314 rdf:type schema:CreativeWork
315 https://doi.org/10.1086/428909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028529523
316 rdf:type schema:CreativeWork
317 https://doi.org/10.1089/104454902760330138 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059203768
318 rdf:type schema:CreativeWork
319 https://doi.org/10.1089/aid.1990.6.1257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078210218
320 rdf:type schema:CreativeWork
321 https://doi.org/10.1093/nar/gkg519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040615905
322 rdf:type schema:CreativeWork
323 https://doi.org/10.1097/00002030-199712000-00006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037096984
324 rdf:type schema:CreativeWork
325 https://doi.org/10.1097/00042560-199512000-00003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050433277
326 rdf:type schema:CreativeWork
327 https://doi.org/10.1097/qad.0b013e3280112cd1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052284168
328 rdf:type schema:CreativeWork
329 https://doi.org/10.1097/qad.0b013e32831392d4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048879950
330 rdf:type schema:CreativeWork
331 https://doi.org/10.1110/ps.4210102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000906630
332 rdf:type schema:CreativeWork
333 https://doi.org/10.1126/science.2832944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062564672
334 rdf:type schema:CreativeWork
335 https://doi.org/10.1126/science.2990040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062580015
336 rdf:type schema:CreativeWork
337 https://doi.org/10.1128/jvi.77.5.3157-3166.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048595361
338 rdf:type schema:CreativeWork
339 https://doi.org/10.1371/journal.pbio.0020333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004041016
340 rdf:type schema:CreativeWork
341 https://doi.org/10.4049/jimmunol.0711406 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032416900
342 rdf:type schema:CreativeWork
343 https://doi.org/10.4049/jimmunol.168.1.197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074964103
344 rdf:type schema:CreativeWork
345 https://www.grid.ac/institutes/grid.12711.34 schema:alternateName University of Urbino
346 schema:name Department of Molecular Sciences, University of Urbino “Carlo Bo”, Via Saffi 2, 61029, Urbino, Italy
347 rdf:type schema:Organization
348 https://www.grid.ac/institutes/grid.416651.1 schema:alternateName Istituto Superiore di Sanità
349 schema:name Centro Nazionale AIDS, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy
350 rdf:type schema:Organization
351 https://www.grid.ac/institutes/grid.5606.5 schema:alternateName University of Genoa
352 schema:name Department of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genova, Viale Benedetto XV 7, Genova, Italy
353 rdf:type schema:Organization
354 https://www.grid.ac/institutes/grid.8404.8 schema:alternateName University of Florence
355 schema:name Centro Risonanze Magnetiche, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino Florence, Italy
356 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...