Non-enzymatic phosphorylation of bovine serum albumin by Cr(V) complexes: Role in Cr(VI)-induced phosphorylation and toxicity View Full Text


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Article Info

DATE

2005-07

AUTHORS

Chellappa Vasant, Sundararaj Sankaramanivel, Mahadevan Jana, Rama Rajaram, Thirumalachari Ramasami

ABSTRACT

Evidence for the non-enzymatic phosphorylation of bovine serum albumin (BSA) by sodium bis(2-ethyl-2-hydroxybutyrato)oxochromate(V), Na[CrVO(ehba)2], 1, sodium bis(2-hydroxy-2-methylbutyrato)oxochromate(V), Na[CrVO(hmba)2], 2 and potassium dichromate, K2Cr2O7, 3 in the presence of labeled adenosine-5'-triphosphate (ATP) under conditions of physiological pH is presented. Aggregation and extent of phosphorylation of BSA mediated by 1, 2 or 3 seems to increase with the concentration and time of incubation of the reaction mixture containing all the reactants. The [gamma-32P] label in ATP is incorporated into aggregates of BSA in the in vitro reaction of the protein with ATP in the presence of 1, 2 or 3. Phosphorylation of BSA by ATP in the absence of 1, 2 or 3 is negligible. Addition of EDTA reverses aggregation of protein and liberates partially the incorporated phosphate label. The stoichiometry of phosphorylation is found to be the highest and is equal to 12.25 mol PO4(3-)/mol BSA in the presence of 500 microM of 1, which decreases to 10.56 mol PO4(3-)/mol BSA after EDTA treatment. Resistance to the removal of phosphate label by EDTA increases with increase in time of incubation. Dialysis of phosphorylated BSA reverses the incorporated [gamma-(32)P] label only partially, indicating the formation of covalent links of phosphate groups to BSA. Evidence for the site of phosphorylation in the reaction mediated by 1, 2 or 3 being hydroxyl side groups of tyrosine and serine/threonine residues has been gained. Based on the results, a possibility that 1, 2 and 3 mimic the function of tyrosine and serine/threonine kinases has been invoked. More... »

PAGES

153-164

References to SciGraph publications

  • 1993-03. Comparison of the uptake and distribution of chromate in rats and mice in BIOLOGICAL TRACE ELEMENT RESEARCH
  • 2007-10-25. Metabolism of the carcinogen chromate by cellular constituents in INORGANIC ELEMENTS IN BIOCHEMISTRY
  • 2002-05. Molecular mechanisms of Cr(VI)-induced carcinogenesis in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 2004-04. Caspase-3: Its potential involvement in Cr(III)-induced apoptosis of lymphocytes in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11010-005-1206-5

    DOI

    http://dx.doi.org/10.1007/s11010-005-1206-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1021245440

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16335795


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