Ontology type: schema:ScholarlyArticle
2019-03
AUTHORSByoung Kwon Park, Avishekh Gautam, Sony Maharjan, Su In Lee, Younghee Lee, Hyung-Joo Kwon
ABSTRACTThe c-Myc protein is diversely involved in normal cellular function, and its deregulation has been implicated in several cancers. Therefore, c-Myc is a validated target for anti-cancer therapeutics. Here, we developed a monoclonal antibody specific to an epitope involved in the protein–protein interaction and DNA binding of c-Myc. We selected two candidate epitopes based on the antigenicity prediction and 3-D structure of c-Myc and successfully obtained a c-Myc-specific monoclonal IgG2a antibody clone. Based on the immunoprecipitation assay, the antibody recognized overexpressed c-Myc as well as endogenously expressed c-Myc in cell lines. The antibody co-immunoprecipitated Max showing that the antibody can recognize the c-Myc-Max heterodimer in solution. The results from the ELISA-based DNA binding assay revealed that treatment with the monoclonal antibody inhibits the binding activity of c-Myc. Therefore, we suggest that our anti-c-Myc monoclonal antibody can be used as a starting material for the development of an anti-cancer drug targeting c-Myc. More... »
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